ObjectiveTo compare the outcomes of standard large craniectomy with routin craniectomy in 65 patients with severe traumatic brain injury. Methods65 patients were randomly divided into standard large craniectomy group(41 cases) and routine craniectomy group (24 cases), and the effect was compared between the two groups. ResultsThe outcome showed 17 cases of good recovery,6 cases of moderate disability,5 cases of severe disability,2 cases of persistent vegetative status,l1 cases of death in the standard group and 5 eases of good recovery,3cases of moderate disability,4 cases of severe disability, 1 of persistent vegetative status, 12 cases of death in route group. ConclusionThe standard flesh wound big bone petal reduced pressure technique could enable the brain organization to obtain the full reduced pressure, effectively protect the brain function, after the distinct improvement heavy eranioeerebral damages, reduce the case fatality rate. But, this method also had certain shortcoming, must therefore grasp the surgery to refer to drafts.

Objective To evaluate the change in the expression of ATP-sensitive potassium (KATP) channels in spinal dorsal horn neurons in rats with chronic post-thoracotomy pain (CPTP). Methods Twenty male SD rats, weighing 250-350 g, were randomly divided into 2 groups ( n = 10 each): sham operation group and CPTP group. The animals were anesthetized with 10% chloral hydrate 300 mg/kg, tracheostomized and mechanically ventilated. At 1 d before operation, and at 1, 5, 10, 15, 20, 25 and 30 d after operation, paw withdrawal threshold to yon Frey filament stimulation was measured and the acetone test was performed. The responses of the hindpaw to the acetone test within 1 min were recorded. The rats were sacrificed at 30 d after operation, and the cervical and upper thoracic segments of the spinal cord were removed to detect the expression of KATP channels in spinal dorsal horn neurons by immuno-histochemistry. Results Compared with sham operation group, mechanical pain threshold was significantly decreased at 5-30 d after operation, the response frequency was significantly increased at 20-30 d after operstion, and the expression of KATP channels in spinal dorsal horn neurons in the thoracic segments was down-regulsted at 30 d after operation in CPTP group ( P ＜ 0.05). Conclusion The expression of KATP channels in spinal dorsal horn neurons is down-regulated in rots with CPTP,which may be involved in the pathophsiology mechanism of CPTP.

Objective To investigate the diagnostic value of the real-time four-dimensional ultrasound imaging examination in the diagnosis of fetal malformations.Methods Four-dimensional ultrasound prenatal audio and video materials,1 008 cases of 12～20 weeks pregnant were retrospectively analyzed,and the dignostic value for the fetal malformation Was obser red by following-up.Results Four-dimensional ultrasound detection of fetal malformations in 26 cases,27 cases of confirmed postpartum fetal malformations,the rate was 96.3%,1 case of missed diagnosis.missed diagnosis rate was 3.7%misdiagnosis rate was 0.Conclusion The dynamic continuous four-dimensional ultrasound could show the activities of the fetus in uterus,diagnosis of most of the morphological structure of fetal malformations,reduced birth defects in children.

It is reported that more than 95% microbe species non-cultured or un-cultured yet in now days by the traditional cultivation approach, which limits discovering novel bioactive compounds from microorganisms. ‘Metagenome’ is the genomes of the total microbiota found in nature. Metagenome libraries were constructed by directly extracting DNA from environmental sample and transforming to surrogate host. The libraries were screened for novel bioactive compounds or genes surrounding their synthase in different strategies of function-driven or sequence-driven. These have enormously amplified the space of microbial resource utilization and enhanced the opportunity of obtain novel bioactive compounds.

0.05).Conclusion IPAT sequence can gain super images in less time with more coils.IPAT sequence needs the distance between coil and checkpoint larger than 5 mm.MRI scan for vertebra just fit it.We think cutting scan time is a direct and effective method to decrease constructed defect.

Objective To analyze the use of serum pepsinogen(PG) and Helicobacter pylori(Hp)-IgG antibody in the screening of atrophic gastritis.Methods A total of 222 patients were divided into two groups according to the gastroscopy results:159 cases in observation group(atrophic gastritis) and 63 cases in control group(non-atrophic gastritis).Patients in observation group were divided into gastric antrum group(50cases),gastric body group (71 cases) and full stomach multifocal group (3 8 cases) according to shrinking parts.The serum PG Ⅰ and PG Ⅱ was determined by enzyme linked immunosorbent assay method,and PGR (PG Ⅰ / PG Ⅱ) was calculated.The serum Hp-IgG antibody was examined by qualitative analysis.Results The serum PG Ⅰ and PGR was (57.82 ± 23.15) μ g/L,4.41 ± 1.82 in observation group,which was lower than that in control group[(125.04 ± 29.36) μ g/L,10.10 ± 2.01],and there was significant difference (P ＜0.01).The positive rate of the serum Hp-IgG antibody was 86.16％(137/159) in observation group,which was higher than that in control group [53.97％ (34/63)],and there was significant difference (P ＜ 0.01).There was significant difference in the serum PG Ⅰ and PGR between gastric antrum group and gastric body group,full stomach multifocal group (P ＜ 0.01).There was no significant difference in the serum PG Ⅰ and PGR among different atrophy degree in gastric antrum group(P＞ 0.05),but there was significant difference in the serum PG Ⅰ and PGR among different atrophy degree in gastric body group and full stomach multifocal group (P ＜ 0.01).Conclusions The serum PG Ⅰ and PGR and Hp-IgG antibody is related to atrophic gastritis.They are helpful to the screening of atrophic gastritis.

Effect of saikosaponins on sleep [slow wave-stage Ⅰ (Ⅰ SWS), slow wave -stage Ⅱ (Ⅱ SWS)rapid eye movement, REM] were studied in cats. Electrophysiological recording of EOG,EEG, EMG and PGO were monitored by polygraph for six consecutive hours. Preparation of saikosaponins was injected intraperitoneally. Results showed that saikosaponins could prolong total sleeping time, Ⅱ SWS and REM time. The effect of saikosaponins is better than "zhushaanshen pill" by gastric gavage.

Objective:Endoscopic retrograde cholangiopancreatography (ERCP) and sphincterotomy (EST) are well accepted as the procedure of choice for diagnostic and therapeutic purposes of pancreaticobiliary disease. The aim of this study is to investigate the curative effect and complication of pancreaticobiliary disease managed by therapeutic ERCP. Methods: A total of 59 patients with common bile duct stone,acute obstructive suppurative cholangitis,malignant bile duct obstruction, acute or chronic pancreatitis,and sphincter of Oddi dysfunction. Each case was confirmed by ultrasonographic examination, diagnostic ERCP and blood amylase test. All patients underwent therapeutic ERCP such as EST, stone extraction and drainage. Results :Of the 59 patients, 16 patients with acute obstructive suppurative cholangitis and severe acute pancreatitis underwent urgent management of endoscopic intervention, stone extraction and endoscopic nasobiliary drainage. The remaining 43 patients underwent diagnostic ERCP,followed by therapeutic ERCP in cases of documented bile duct stone,sphincter of Oddi dysfunciton,or chronic pancreatitis. The success rate of endoscopic procedure was 100% ,and the complication rate was 6. 8% ,without mortality. Conclusion : In patients with pancreaticobiliary disease , therapeutic ERCP provides adequate management and is associated with low complication rates.

Objective:To investigate the effect and mechanism of rh-LIF on proliferation and differentiation of HL-60 cell line.Methods:HL-60 cells were treated with different dosage of rh-LIF for 3 to 6 days,cell cycle was analysed by flow cytometry,expression of P53 and P21 protein were observed by immunohistochemistry and P53 mRNA were assayed by in situ hybridization.Results:HL-60 cell line could differentiate into monocyte and macrophage cell line in the presence of rh-LIF.The proliferation of HL-60 cells were inhibited in the presence of rh-LIF,the cells stagnated at G 1 stage,while expression of P53,P21 protein and P53 mRNA were increased obviously.Conclusion:The effects of rh-LIF on the proliferation and differentiation of HL-60 cells may be associated with the increase of P53,P21 protein and P53mRNA.

Pterygium is a common ocular surface disorder that has high rate of prevalence and recurrence. Currently, its pathogenesis is still unclear. Scholars agree that it's relevant to joint action of many factors. With the development of molecular biology, genetic and molecular studies concerning the pterygium achieved a breakthrough progress. Now the development of study in the pathogenesis of pterygium is reviewed in this paper.