OBJECTIVE:To evaluate the effect of bone cement prosthesis by retrieving literatures and clinical validations.METHODS:With the key words"total hip replacement,prosthesis,bone cement"in Chinese and English,articles were searched in VIP and Pubmed databases published between January 1998 and December 2009 Articles related to cemented and cementedless prosthesis were included Repetitive articles and Meta analysis were excluded.The questions were discussed,including classification of artificial joint replacement,development of cemented hip prosthesis materials,treatment effect and clinical validation of cemented hip prosthesis materials,anatomic features of calear femorale and operation of hip Joint replacement.In addition,55 cases undergoing total hip replacement with cemented prosthesis in Qiannan Prefecture People's Hospital were analyzed.RESULTS:Artificial joint replacement includes cemented and cementedless prosthesis.Cemented prosthesis is applicable for ostecoporotic and elderly patients.Cementedless prosthesis can be used for middle-aged or young patients.As the shear strengtn Of metal and bone cement surface is bad,novel bone cement modifies the prosthesis surface to enhance the shear strength Clinical results showed that during the follow up of 12 months to 6 years,there were 39 cases of excellent,13 of good and 3 of fair according to Harrisyg scores.Postoperative X-ray showed hip pain in 3 patients,prosthesis prolapse in 1,prosthesis loosening in1 and dislocation in 1.No infection or femoral fracture was detected.CONCLUSION:With development of various novel bone cement materials,the performance of bone cement is improving,and may become ideal bone substitutes.

Objective:To choose a best method of estimating the amount of blood loss after cesarean deliveryby comparing of three methods,to analyze the related factors of the amount of the blood loss more than 500ml after cesarean section.Methods:We collected the clinical data of the patients who were accepted cesarean delivery and treated in our hospital between January 2014 and December 2014.We estimate the amount of blood loss by different methods(including visually estimated,hematocrit (HCT) change and formula).Based on the formula method as the gold standard,we analyze the risk factors of the patients whose blood loss were more than 500 ml.Results:Among the 583 patients,using the method of visually estimated,there were 23 cases whose blood loss were more than 500 ml(3.95％),using the method of formula,there were 133 cases whose blood loss were more than 500 ml (22.81％),using the method of HCT change,there were 116 cases whose blood loss were more than 500 ml(19.90％).The incidence of patients whose blood loss were more than 500 ml between HCT change and formula method showed no significant difference(P ＞ 0.05),but the incidence by visually estimated method was less than the two other methods (P ＜ 0.05).We found the value of HCT change was a statistically significant positive correlation with the amount of blood loss more than 500ml.When the blood loss was more than 500 ml,the accuracy and sensitiveness of HCT method were significantly higher than those of visually estimated method(76.69％,87.93％ vs 12.78％,73.91％,respectively).Abnormal labor,uterine leiomyoma,placental factors,and macrosomia were risk factors of cases whose blood loss more than 500ml.Conclusions:HCT method is similar in accessing the blood loss after cesarean section with formula method,especially in the cases whose blood loss was more than 500 ml;HCT method is more convenient than formula method,and more accurate than visually estimated method.HCT method can be used as the standard for estimating blood loss initially,and formula method should be used as the method for estimating the blood loss accurately.Patients with macrosomia and abnormal labor are more likely to occur massive hemorrhage after cesarean section.

To introduce professor YAN De-xin's experience in diffrerentiating and treating Emeda with Heart Involved from qi and flood He thinks that pathogenesis of Emeda with Heart Involved is water overflowing due to yang deficiency,internal retention of blood stasis and turbidity.So this disease can be differentiated from these aspects including the disturbance of the functional activities of the lung,failure to transform body fluid;body fluid transformed by blood stasis,simultaneous disorders of both blood and body fluid;deficiency of pectoral qi,derangement of qi and blood.What's more,he proposes the application of drugs for warming yang and promoting blood circulation is very important.

The rapid advances over the past decades in molecular biology led to new ideas and methods to investigate the mechanism of depression, which enlarged the views of fields from neurological transmitters and related receptors to post-receptor signal transduction levels. It not only help to elucidate the pathogenesis of depression, but also offer beneficial evidence for development of new antidepressants. This paper reviews the development of molecular biological study in depression.

As an important way to maintain the internal environment of the human body, the renin-angiotensin system (RAS) plays an important role in the diabetic cognitive dysfunction, which may associate with insulin resistance, inflammatory reaction, oxidative stress and other mechanisms. Traditional Chinese Medicine can improve cognitive function of diabetes, in which RAS may play a role.

Objective To explore the effect of osteopontin(OPN) as marker in colon cancer tissue and analyze the relationship between osteopontin and recurrence.Methods ELISA method was used to determine the serum levels of osteopontin in 58 patients with colon cancer(observation group) and 22 cases of normal subjects(control group);semi-quantity RT-PCR was used to detect the expression of osteopontin in tissues.The expression level of OPN mRNA in colon cancer tissue and serum OPN level in 23 recurrence cases and 35 non-recurrence cases one year after operation were observed.Results The OPN mRNA expression in colon cancer tissue and the serum osteopontin level in patients in observation group were higher than those in control group(P

With the development of genomics , bioinformation , engineering , computer science and other fields, it has witnessed explosive growth of molecular diagnostic technologies.More and more technologies are used in desease diagnosis , therapy and prevention , which have presented a huge opportunity and challenge for clinical laboratory.Each technology has corresponding field of application , so it is a crucial problem for clinical laboratory to select appropriate molecular diagnostic technology for personalized medicine.

Objective To investigate the diagnostic value of Lugol solution staining combined with nar-row band imaging (NBI)for esophageal cancer and precancerous lesions.Methods From December 201 3 to December 2014,1 87 patients who were with suspicious esophageal lesions and stained with Lugol solution under endoscopy were selected as control group,while from January 2015 to June 2015,124 patients who were with sus-picious esophageal lesions and examined with Lugol solution staining and NBI under endoscopy were selected as observation group.The sensitivity,specificity and accuracy between the two groups were compared and analyzed. Results One hundred and four cases (75.0%)were found among observation group,which was higher than 93 cases (55.6%)among control group (χ2 =12.07,P <0.01 ).The total detection rates of esophageal cancer and high grade intraepithelial neoplasia (HGN)were 29.0% and 17.7% in observation group respectively,which were higher than 1 8.2% and 6.4% in control group (χ2 =4.15,P =0.04;χ2 =8.54,P <0.01 ).The Kappa value in observation group was 0.55,which was higher than 0.36 in control group.The sensitivity and accuracy in observation group were 89.9% and 82.3% respectively,which were significantly higher than 71 .8% and 68.4% in control group (χ2 =9.79,P <0.01 ;χ2 =7.38,P <0.01 ).Conclusion The sensitivity and accuracy of endoscopic Lugol solution staining combined with NBI for esophageal cancer and precancerous lesions are higher than Lugol solution staining,and it is worthy to popularize the application of Lugol solution staining com-bined with NBI in diagnosing esophageal cancer and precancerous lesions.

Objective To compare the the curative effect of laparoscopy and open operation in surgical repair of gastric perforation.Methods The clinical data of 68 patients with gastric perforation were analyzed.According to different operation method,the patients were diviede into laparoscopic group and laparotomy group,34 cases in each group.The patients in the two groups were given laparoscopy or laparotomy,respectively.ResultsThe operation time between the two groups had no statistically significant difference (P＞0.05).The intraoperative blood loss,postoperative exhaust time,length of hospital stay in the laparoscopic group were (24.9±7.3)mL,(31.9±10.5)h,(7.4±2.9)d,respectively,which were significantly less than those in the laparotomy group,the differences were statistically significant (t=9.953,5.226,7.441,all P＜0.05).The incidence rate of postoperative complications of the laparotomy group was 32.35％,which was significantly higher than 8.82％ of the laparoscopic group,the difference was statistically significant(χ2=5.757,P=0.016).Conclusion The curative effect of laparoscopic downward gastric perforation repair is superior to laparotomy,patients have rapid recovery,less complications,it is worthy of clinical popularization and application.

BACKGROUND:The chronic wounds, also cal ed non-heading wounds, can seriously affect the quality of life of patients and has brought heavy burden to patients, as wel as health care professionals. celltherapy is a new method for promoting wound healing and plays an important role in the repair of chronic wounds.
OBJECTIVE:To summarize the progress of researches on the chronic wound healing mechanism and celltherapy, and to provide evidences for the clinical management of chronic wounds and relative basic researches.
METHODS:A computer search of CNKI database from 2005 to 2012, PubMed database from 1995 to 2012 and Foreign Medical Journal Ful-Text Service database from 2000 to 2012 was performed using“non-healing wounds, diabetic foot ulcer, wound healing, celltherapy”in Chinese and English as the key words to retrieve articles about chronic wound healing mechanism and the application of celltherapy. Total y, 42 articles meeting the inclusive criteria were included in result analysis.
RESULTS AND CONCLUSION:The wound healing is a complex biological process, involving multiple celltypes, extracellular matrix and cytokine factors. The delayed healing of refractory wound seriously affects the quality of life of patients and has brought heavy economic burden to patients. At present, many methods have been employed to promote wound repair, such as local hyperbaric oxygen therapy, surgical treatment, herbal Chinese medicine, application of various growth factors, celltherapy and gene therapy. celltherapy is noninvasive and those delivered cells can adapt to their environment, are able to release growth factors and cytokines, and more importantly, are able to deliver the growth factors for the wound healing process due to cellsignaling capabilities. Currently, cells used for the treatment of chronic wound cells mainly include bone marrow stem cells, bone marrow-derived mesenchymal stem cells, cord blood stem cells, peripheral blood stem cells, epidermal stem cells, skin-derived progenitor cells, adipose stem cells, fibroblasts and platelets.