Objective To investigate the predictive factors of acute pancreatitis for patients with gallstone.Methods One hundred and eighteen patients with gallstones who were admitted to Department of Gastroenterology,People's Hospital of Wuhan University were selected,the gallbladder size,number and size of gallstones,common bile duct stones were determined by imaging study.Then the patients were grouped according to the complication of acute pancreatitis.Results Sixty-one cases of acute pancreatitis occurred out of 118 patients.The gallbladder size of 74 patients was normal,and among them 49(66.2％) developed acute pancreatitis; the gallbladder size of 44 patients was increased or decreased,and among them 12(27.3％)developed AP.Thirty-one patients had a single gallstone,and 11 (35.5％) developed AP; while 87 patients had multiple gallstones,and 50 (57.5％) was complicated with AP.Among patients with AP,11 patients had a single gallstone,and the size of the gallstone in the 8 patients (72.7％) was ≥10 mm; while 50 patients had multiple gallstones,and the size of the gallstone in the 41 patients (82.0％) was ＜ 10 mm.Nineteen patients were complicated with common bile duct stones,and 17 (89.5％) developed AP; while 99 patients were not complicated with common bile duct stones,and 44 (44.4％) developed AP.The difference between the two groups was statistically significant (x2 =16.758,P =0.000 ; x2 =4.425,P =0.029 ; x2 =13.434,P =0.001 ; x2 =12.994,P =0.000).Conclusions Acute biliary pancreatitis is associated with gallstones.The size of gallbladder,size and number of gallstone,as well as the presence of common bile duct stones are related to the development of AP.

Objective To explore the feasibility of the hydrogel as a cell carrier for parathyroid cells.Methods Cryopreserved parathyroid cells were recovered and cultured until sufficient,then the cells were digested、centrifuged and pipetted to single-cell suspension.The chitosan solution,crosslinking agents and cell suspension were mixed to hydrogels by some ratio.After that,hydrogels covered by complete medium were incubated at 37 ℃ in constant temperature incubator.Meanwhile,the supernatant were collected for measuring the PTH level every other day and the viability and shape of cells in hydrogels was also evaluated by fluorescopy.Results Parathyroid cells were recovered and cultured successfully and the adherent cells were spindle-like.The hydrogel could be prepared at room temperature in less than 1 minutes.The fluorescent confocal microscopy showed that more than 90％ of the cells in the hydrogel kept viable after 24 hours and the proportion had no significant change after 3 days.Furthermore,PTH was detected in the supernatants.Conclusion The facilely prepared inexpensive hydrogel,which can maintain normal morphlology and function of cells,is of potential application to cell transplantation therapy for hypopatathyroidism.

Objective To investigate the correlation between the coagulation function and fibrinolytic activity with deep venous thrombosis (DVT) after traumatic fracture operation.Methods One hundred and thirty-four cases of traumatic fracture from April 2010 to May 2014 were divided into the DVT group(24 cases) and non-DVT group,and at the same time 110 healthy people were selected as the control group.The venous blood at different time points was collected for observing the levels change of prothrombin time (PT),activated partial thromboplastin time (APTT),fibrinogen (FIB),platelet aggregation rate (PAgT),dipolymer (D-D),thrombin antithrombin complex (TAT),plasminogen activity (PIg) by using appropriate methods.Results There was no difference in APTT and PT groups among the groups(P>0.05).Preoperative FIB,PAgT,D-D and TAT levels was the DVT group >non-DVT group > control group,while the level of PIg was the DVT group0.05).Conclusion The increase of D-D and TAT is closely related with DVT in traumatic fracture and,which is an independent risk factor.

Obesity is the most important risk factor of primary hypertension. Rapid increase of obesity-related hypertension has become a worldwide problem. But the specific mechanism of the condition remains to be fully understood. This paper reviews the latest literatures and tries to explain the mechanism of obesity-related hypertension from the following four aspects: hypoadiponectinemia, leptin and resistance of leptin, insulin and resistance of insulin and the activation of renin-angiotensin system(RAS).

In order to explore the significance of cardiomyocyte apoptosis in coronary heart disease (CHD), the TUNEL and transmission electron microscopic (TEM) techniques were performed. The results showed that the permillage of cardiomyocyte apoptosis in infarct area in patients of sudden death from CHD was significantly higher than that in control group (557.94? 144.10‰ VS 34.30?20.68‰, p

Objective To investigate the changes of actin (HHF35), serum albumin (A1), fibronectin (Fn) in cardiac cells and cardiac conducting sysytem (CCS) in order to probe into postmortem diagnosis of sudden manhood death syndrome (SMDS). Methods Immunohistochemical detections of actin, serum albumin and fibronectin (Fn) in the cardiac cells and CCS were performed by S-P method in cases with SMDS and cases died from coronary heart disease and non-cardiovascular diseases which were used as the control. Results Of the 22 cases with SMDS, 17 were found of actin-negative staining, 18 serum albumin-positive and 15 Fn-positive staining with evident pathological lesions in CCS in 8 cases. Conclusion It is suggested that SMDS is associated with early myocardial ischemia or infarction, which results in abnormality in actin, serum albumin and fibronectin (Fn) in the cardiac cells and CCS.

Objective To collect the information about the types and clinically-used materials for fixed partial dentures, and evaluate the current conditions and characteristics of fixed partial dentures in Shanghai. Methods Three municipal public hospitals and two private commercial dental labs in Shanghai were served as sample units. The data of fixed partial dentures from January 2005 to December 2007 were retrospectively analysed. The number, type and material of dentures of each year were observed, and the proportion of different types and materials of dentures were analysed. Results From 2005 to 2007, the total number of fixed partial dentures gradually increased year by year, and porcelain fused metal crowns accounted for the largest proportion. The application of noble crown in public hospitals increased annually, and the increase rates were 13.85% in 2005 to 2006 and 30.86% in 2006 to 2007, respectively, while the application of casting basic metal full crown decreased annually. Computer-aided design and computer-aided manufacture (CAD/CAM) fixed partial dentures (CAD/CAM crown) and implant dentures occupied a tiny percentage of total fixed partial dentures in public hospitals and privated dental labs, while both had a remarkable increase trend in number between 2005 and 2007 (227.78% and 53.01%, respectively for 2005 to 2006, and 120.34% and 27.41%, respectively for 2006 to 2007). Conclusion Fixed partial dentures are the most common prostheses for tooth defect, and are characterized by the growing application of advanced techniques and high-quality materials.

Animals ; Blood Pressure ; Heat-Shock Proteins ; metabolism ; Heat-Shock Response ; Hypotension ; metabolism ; Male ; Myocardium ; metabolism ; Nitric Oxide ; metabolism ; Rats ; Rats, Sprague-Dawley

Objective To investigate temporal expression of estrogen receptor (ER) in myocardium at non-infarct zone after acute myocardial infarction (AMI) in rats. Methods 120 healthy, male SD rats were randomly divided into three groups: control group, left anterior descending coronary artery (LAD) occlusion group and sham operation group with 8 rats in each group. The murine AMI-model was established by LAD ligation after anesthetization, and the rats in both LAD occlusion group and sham operation group were sacrificed at 2h, 4h, 8h, 12h, 1d, 2d, 4d, and 9d after occlusion. Myocardial samples were collected. H.E and ER immunohistochemical staining were performed. The results were quantitatively evaluated by image analysis system. The data were statistically analyzed with SPSS for Windows. Results ER expression in non-ischemic cardiomyocytes after LAD occlusion became more prominent with extension of LAD occlusion period. There was no significant difference within 12h after AMI, while ER expression was enhanced significantly 24h after ischemia. The enhancement were more evident from 4 to 9 days. Conclusion The results suggested that protective role may be initiated in response to acute ischemia in cardiac cells at non-infarct zone by increased ER expression after LAD occlusion in rats.

Aim To study the active constituents for the treatment of rheumatoid arthritis from the ethyl acetate extracts of the roots of Lasianthus acuminatissimus Merr. Methods Various chromatographic techniques were used to separate and purify the constituents. Their structures were established on the basis of 1D, 2D NMR and HRMS spectroscopic analyses and their preliminary evaluation of anti-inflammation effect on the release of β-glucuronidase was carried out. Results Eight compounds were isolated and identified as lasianthuslactone A ( 1 ) , codonolactone ( 2 ), 2, 5-dimethoxy-1,4-benzoquinone ( 3 ) ,uncargenin A (4) , nonadecyl alcohol (5) , 13-docosenoic acid (6) , tetracosanoic acid (7) and β-sitosterol (8). Compound 3 showed a significant inhibitory effect on release of β-glucuronidase rat polymorphous nuclear leukocytes activated by platelet activating factor (PAF). Conclusion Compound 1is a new one, the others were isolated from the plant for the first time and 3 is one of active antiinflammation compound in the plant.