Objective To observe the clinical effect and the incidence of neuralgia of acupoint injection with nerve growth factor onRamsay Hunt syndrome. Methods 65 patients with Ramsay Hunt syndrome were divided into treatment group (n=33) and control group(n=32). All the patients were treated with the anti-virus and glucocorticoid medicine, and were acupunctured at the similar points with electroacupuncturewith the disperse dense wave. The treatment group was injected with mouse nerve growth factor at the acupoints. 2 days offevery 5 times, 10 times as a course for 2 courses. The treatment effect and the incidence of sequelae neuralgia after 3 months were compared.Results The total effective rate of treatment group (93.9%) was significantly higher than control group (71.9%) (P<0.01). The incidenceof sequelae neuralgia of the treatment group (6.3%) was significantly lower than the control group (26.7%) (P<0.01). ConclusionAcupoint injection of mouse nerve growth factor can improve the efficacy and reduce the incidence rate of sequelae neuralgia in patientswith Ramsay Hunt syndrome.

Objective To study the application of waveforms in treatment of peripheral facial paralysis with electroacupuncture. Methods 66 patients with peripheral facial paralysis were divided into two groups: treatment group (34 cases) and control group(32 cases). All the patients were acupunctured at the similar points with electroacupuncture, while the disperse-dense wave and the discontinuous wave were used successively in the treatment group, and the disperse-dense wave was used alone in the control group. Results There were significant differences between the two groups in the total effective rate and the days for cure (P<0.01). Conclusion The effect of alternate disperse-dense wave and discontinuous wave is superior to that of disperse-dense wave alone in electroacupuncture treatment for peripheral facial paralysis.

Objective To explore the size changes of erythrocytes infected with Plasmodium malariae,so as to improve the basic?level experimenters’microscopy capabilities for P. malariae identification in thin blood smears. Methods The micro?scopic features of erythrocytes infected with P. malariae in thin peripheral blood smears were observed,and a microscope image processing software was used to measure and analyze the diameter changes of the erythrocytes infected with P. malariae. Re?sults The diameter of erythrocytes infected with P. malariae decreased significantly compared with that of the normal erythro?cytes. The three parameters in this study:the diameter of erythrocytes,the value of diameter variation,and the ratio of diameter variation varied at different developmental stages of P. malariae,and the differences were statistically significant(all P<0.01). The variances of the three parameters grouped by different cases or different developmental stages in different cases were ana?lyzed,all showing statistically significant differences(all P<0.01). Conclusion All the developmental stages of P. malariae will cause the decrease of the diameters of infected erythrocytes in peripheral blood smears,but the influence on the diameter of erythrocytes,value of diameter variation,and ratio of diameter variation varies at different developmental stages in different cases.

Objective To introduce the recent studies about the lymph node-targeted chemotherapy for gastric carcinoma. Methods The literatures on the lymph node-targeted chemotherapy for gastric carcinoma in recent years were collected and reviewed. Results The lymph node-targeted chemotherapy for gastric carcinoma was effective because it couled improve the drug concentrations in regional lymph node. Conclusion As a part of multiple treatments for gastric carcinoma, lymph node-targeted chemotherapy will be further developed.

Pesticides plays an important role in the history of agricultural development,but the health hazards and environmental pollution problems are attracting increasing attention. Methyl parathion is one of the most widely used pesticides,its threat to human health caused increased emphasis. This paper introduced the chronic toxicity of methyl parathion to various systems such as the nervous system,endocrine system,immune system and reproductive system.

Objective To investigate the effective regime for treating keloid to dispel clinical symtoms and reduce postoperative relapse. Methods Laser of carbon dioxide was applied to remove keloid, and then the wound was dressed with wet dressing of Danshen for 2～3 weeks. After the wound epithelized, the base and edge of the wound was injected with Danshen solution for 2～3 months, and the wound was dressed with silica gel per day for 6 months at the same time. The patients were followed up for 1～2 years. Results The curative rate, obviously effective rate and total effective rate were 60.47%, 27.91% and 88.37%, respevtively, in the twenty-four patients with forty-three pieces of keloid. Conclusion The combination therapy for keloid was simple, effective, without side effect and suitable for local keloid of smaller than 15cm 2 in area.

Treated uroschesis due to spinal cord injury in 32 cases with acupuncture therapy (acupuncture group) and compared with Neostigmine injection (control group). In acupucture group, 10 cases got clinical recovery, 17 efficacy and 5 inefficacy, with an effective rate of 84.4%; among 32 cases in control group, 3 cases got clinical recovery, 15 efficacy and 14 inefficacy, the effecrive rate was 56.2%.

Objective To investigate the correlations of serum markers of hepatitis B virus,HBV DNA load and liver function indexes[alanine aminotransferase(ALT)and aspartate transaminase(AST)]in the peripheral blood.Methods Clinical data of 483 patients who were diagnosed with chronic hepatitis B and treated between March 2014 and March 2016 in Tongji hospital were retrospectively analyzed.The serum markers of hepatitis B virus were quantitatively detected by chemiluminescent microparticle immunoassay.Serum HBV DNA load was measured by real-time fluorescence quantitative PCR,and ALT and AST by continuous ultraviolet monitoring.Results There was no correlation between the HBsAg content and HBV DNA load or the rates of abnormal ALT(>41 U/L)and abnormal AST(>35 U/L)(P>0.05).The HBeAg content was not correlated with HBV DNA load and the rates of abnormal ALT(P>0.05),but weakly with the rate of abnormal AST(r=0.21,P<0.01).Although the anti-HBe content was not correlated with the rate of abnormal ALT(P>0.05),it was weakly related to HBV DNA load and the rates of abnormal AST(r=0.16,P<0.05;r=0.19,P<0.01).The anti-HBc content had weak correlations with HBV DNA load,the rates of abnormal ALT and abnormal AST(r=0.25,P<0.01;r=0.29,P<0.01;r=0.29,P<0.01).The logarithm value of HBV DNA load was weakly positively correlated with ALT and AST(r=0.24;r=0.29).Conclusion Quantitative detection of both serum markers and the DNA of hepatitis B virus can complement each other,and when combined with detection of liver function indexes,it will help understand the damage of liver tissue.

Myoblast transplantation has been carried out for a certain period,but its efficiency in repairing inherited myopathy or severe muscular injury is not satisfactory.The improvements in myoblast culture,transplantation approach,recipient and cell preparation before transplantation,scaffold material and its selection,and body immunity can positively influence transplantation efficacy,and improve recipient function.In addition,these improvements influence the prospect of myoblast transplantation or cell-mediated gene therapy.

OBJECTIVE:To explore the treatment effect and complication prevention of various stent materials for intracranial arteriostenosis.METHODS:A computer-based online search of Science Direct and Ei database (1998-01/2009-10) was performed for English articles with the key words of "intracranial arteriostenosis,stent placement" In addition,CNKI and CBM database (1998-01/2009-10) were searched for related Chinese articles with the same key words in Chinese.Moreover,related works were manually searched.Studies regarding various stent materials for intracranial arteriostenosis were included,including basic and clinical experiments.RESULTS:Multicenter studies of worldwide show that stent implantation displays obvious advantages in treating carotid artery stenosis compared with carotid endarterectomy.Recently,with developing of balloon and stent compliance,as well as stent technology,and application of various novel materials and cerebral protection device,stent has become a safe and effective therapy for intracranial arteriostenosis.However,complications following stent implantation,such as restenosis,ischemic stroke,cerebral hemorrhage hypertransfusion syndrome,and cerebral angiospasm,limit its application.CONCLUSION:The safety and efficacy of intracranial stent implantation remains validation of multicenter and perspective studies.High incidence of restenosis following stent implantation requires further improvement.