Objective To investigate the relationship between serum growth hormone, C-peptide, blood lipids levels, blood glu-cose, hypertension and type 2 diabetes nephropathy. Methods 82 type 2 diabetes (including 40 without nephropathy and 42 with nephropa-thy) and 39 healthy objects were enrolled. BG,GH,C-P,HbAlc,TC,TG,LDL-C and HDL-C were detected. Results The results showed that the values of blood glucose in each time-phase and HbAlc in both type 2 diabetes groups were significantly higher than that in healthy objects. In type 2 diabetes nephropathy group, the serum levels HbAlc, FGH, TC, TG, LDL-C, systolic blood pressure and diastolic blood pressure were significantly higher than that without nephropathy and those healthy objects(P<0.01). C-P (Oh)and C-P (2h)in type 2 di-abetes nephropathy group was significantly lower than that without nephropathy and those healthy objects(P<0.01). In groups patients of type 2 diabetes nephropathy, the disease duration had significantly longer than patients without nephropathy (P<0.01). The results of cor-related analysis with Pearson showed that HbAlc was significantly positive correlated with FGH(r=0.912, P<0.01), HbAlc was signifi-cantly negatively correlated with C-P(0h)(r=-0.803, P<0.01)and HbAlc was significantly negatively correlated with C-P(2h)(r=-0.771, P<0.01). FGH was significantly positive correlated with TC (r=0.413, P<0.05). Conclusion These results suggested that type 2 diabetes nephropathy has persistent elevated levels of blood glucose and disorder of lipid metabolism. The elevated levels of FGH, hy-pertension and islet B cell secretion function deficiency are important risk factors for type 2 diabetes nephropathy.

Objective: To analyse the antinociceptive effect of red nucleus (RN) and its role in the antinociceptive effect of muscle spindle afferents. Methods: The single units of RN or wide dynamic range (WDR) neuron in the spinal cord dorsal horn were extracelluarly recorded. The effects of RN stimulation on nociceptive responses (C-fibers-evoked responses, C-responses) of WDR neurons were observed. The influence of muscle spindle afferents elicited by intravenous administration of succinylcholine (Sch) on the spontaneous discharge of RN neurons and on C-responses of WDR neurons were observed. The effect of muscle spindle afferents on C-responses of WDR neurons after unilateral lesions of RN was also observed. Results: Electrical stimulation of the RN produced a significantly inhibitory effect on the nociceptive responses of WDR neurons. RN neurons were excited by muscle spindle afferents. Muscle spindle afferents significantly inhibited C-response of WDR neurons and this inhibitory effect was reduced by lesions of RN. Conclusion: RN neurons have a significant antinociceptive effect and might be involved in the antinociceptive effects elicited by muscle spindle afferents.

Guidelines as Topic ; Head and Neck Neoplasms ; Humans

Objective To study the level changes and correlation of homocysteine (Hcy) ,high-sensitivity C-reactive protein(hs-CRP) ,cystatin C(Cys-C) and plasma fibrinogen(Fib) in the patients with acute cerebral infarction (ACI) .Methods The fasting blood samples were collected from 178 cases of ACI(ACI group) and 93 healthy individuals blood samples (control group) .The lev-els of serum Hcy ,hs-CRP and Cys-C were detected by the BECKMAN AU-680 fully automatic biochemical analyzer and plasma Fib was determined by the RAC-100 fully automatic coagulometer .Results The levels of serum Hcy ,hs-CRP ,Cys-C and plasma Fib in the ACI group were significantly increased compared with the control group (P<0 .05) .There was significantly positive correlation between Hcy with hs-CRP and Cys-C in the ACI group(r=0 .326 ,0 .361 ,P<0 .05) ,but there was no significant correlation be-tween Hcy and Fib ;there was significantly positive correlation between hs-CRP with Cys-C and Fib(r=0 .365 ,0 .421 ,P<0 .05);the same significant positive correlation also existed between Cys-C and Fib (r=0 .447 ,P<0 .05) .The positive rate of the joint de-tection of Hcy ,hs-CRP ,Cys-C and Fib was 93 .8 % ,which was obviously higher than that of the single indicator detection (P<0 .05) .Conclusion Cys-C ,hs-CRP ,Fib and Hcy participate in the occurrence and development process of ACI ,their joint detection has the important clinical significance for the prevention ,early diagnosis and treatment of ACI .

OBJECTIVE:To establish a HPLC method for the determination of 5-fluorouracil in human plasma METHODS:The plasma samples were extracted with ethyl acetate-isopropanol(85∶15,V/V)following precipitation of plasma proteins with ammonium sulfate The solvent was evaporated to dryness under a stream of nitrogen The dry extract was dissolved in mobile phase and then injected into the Diamonsil C18 column(150mm?4 6mm,5?m)and 0 01mol/L KH2 PO4(pH5 5) was taken as mobile phase The flow rate was 1 5ml/min,UV detection wavelength was 267nm The internal standard was 5-bromouracil RESULTS:The minimal detectable drug concentration in plasma was 0 025?g/ml The calibration curve was linear over a range from 0 3?g/ml to 10?g/ml The intra-day RSD≤5 0%(n=5) and the inter-day RSD≤11 5%(n=5) CONCLUSION:The method can be used for studying the pharmacokinetics and bioavailability of 5-fluorouracil

OBJECTIVE:To establish the quality standard for Prostatitis capsules. METHODS:TLC method was performed to qualitatively identify Pseudostellaria heterophylla,Acortw tatarinowii,Sargentodoxa cuneata,Rubia cordifolia and Vaccaria segeta-lis in the preparation. HPLC method was adopted to determine the content of leonurine in the preparation. The determination was performed on Agilent Eclipse XDB-C18 column with mobile phase consisted of methanol-0.025 mol/L potassium dihydrogen phos-phate(pH adjusted to 2.5)(24 : 76,V/V)with phosphoric acidat the flow rate of 1.0 mL/min. The detection wavelength was set at 277 nm,and column temperature was 30 ℃. The sample size was 10 μL. RESULTS:The TLC spots of P. heterophylla,A. tatari-nowii,S. cuneate,R. cordifolia,and V. segetalis were clear and well-separated without interference from negative control. The lin-ear range of leonurine were 4.05-81.00 μg/mL(r=0.9999). RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The recovery was 98.47%-103.83%(RSD=2.04%,n=9). CONCLUSIONS:Established standard can be used for quali-ty control of Prostatitis capsules.

Drug Resistance, Neoplasm ; Epithelial-Mesenchymal Transition ; Humans ; MicroRNAs ; genetics ; metabolism ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasms ; drug therapy ; genetics ; metabolism ; pathology ; Neoplastic Stem Cells ; metabolism ; pathology

Objective To study the efficacy and toxicity of tetrakis(hydroxymethyl)phosphonium sulfate(THPS)in indoor air disinfection.Methods The orthogonal experiments were employed with three factors including concentration of THPS,temperature and time,and four levels including the concentrations of THPS,0.05%,0.1%,0.5%,1%,and the temperature,15℃,20℃,25℃,30℃,and the time,2 h,4 h,8 h,16 h.The germicidal effect of THPS with those factors was assessed.Using modified method,Daphnia magna straus(Cladocera crustacea),Brachydanio rerio,mice were randomly divided into 5 dose groups and a control respectively.For Daphnia magna straus(Cladocera crustacea),123.03 mg/L,134.90 mg/L,147.91 mg/L,162.18 mg/L and 177.83 mg/L,for Brachydanio rerio,42.66 mg/L,57.54 mg/L,77.62 mg/L,104.71 mg/L and 140.90 mg/L,for mice,183.60 mg/kg,293.75 mg/kg,470.00 mg/kg,752.00 mg/kg and 1 203.20 mg/kg.The data for 2th,4th and 7th day of treatment were collected for calculation of the 48h-EC50,the 96h-LC50 and the acute oral LD50 in Daphnia magna straus(Cladocera crustacea),Brachydanio rerio and mice.Results The maximal germicidal rate of THPS in indoor air was over 86%.At the concentration of 0.5% THPS,4 h reaction time,germicidal efficacy of THPS was optimal.The toxicity test indicated that the acute oral LD50 for mice was 492.72 mg/kg,the 48 h-EC50 for Daphnia magna straus(Cladocera crustacea)was 143.61 mg/L,the 96h-LC50 for Brachydanio rerio was 84.17mg/L.Conclusion The toxicity of THPS is low and can be used for indoor air disinfection with a good efficacy and the potential benefit for environment.

Objective This study was designed to evaluate the biomechanical properties in tendons across the joints when Palmar or dorsal stitches were used. Methods Twenty porcine tendons were transected and then repaired in zone Ⅱand divided into two groups,using modified Kessler by placing the stitches in volar or dorsal part of tendons.The repaired tendons were placed on a joint model with flexion of 60?. An instron tensile testing machine was used to investigate 2 mm gap formation force and ultimate tensile strength. Results 2 mm gap formation force and ultimate tensile strength were both higher when dorsal placement of stitches was used. Conclusions Dorsal placement of stitches was recommended for repairing the tendons across the joints because it can offer greater tensile strength.

Objective To study the preoperative self-efficacy of patients with lumbar disc herniation(LDH) and its influential factors. Methods Seventy-six patients with LDH underwent operation from June 2008 to March 2009 were enrolled in the current study. The patients were investigated with GSES,SAS,SDS and self-made general characteristics before operation. The data were analyzed with SPSS 11.5. Results The score of preoperative self-efficacy was 25. 84 ±6. 76. The course of disease, education level, patients' hobbits could affect self-efficacy (F = 15. 371,P<0.01;F = 18.078,P<0.01;t =2. 368,P<0. 05). Preoperative self-efficacy correlated negatively with the course of disease (r = - 0.541, P < 0. 01). And preoperative self-efficacy correlated with education level and hobbits(r = 0. 589, P < 0. 01; r = 0. 226, P < 0.05) . The score of preoperative dysphoria and depression was significantly higher than norm (P < 0. 01) . Preoperative self-efficacy correlated negatively with dysphoria and depression (r = -0. 494, P < 0.01; r = - 0. 443, P < 0. 01). Conclusions The score of preoperative self-efficacy with LDH is low; the score of preoperative dysphoria and depression is high. Preoperative self-efficacy correlated negatively with the course of disease. And preoperative self-efficacy correlated with education level and hobbits; Preoperative self-efficacy correlated negatively with preoperative dysphoria and depression.