Two different diets(high cholesterol and low protein) were used to induce gallstone formation in guinea pigs. After the guinea pigs had been fed on lithogenie diets for 8 weeks, the rate of gallstone formation was 100% in both groups. However, when ascorbic acid was added in the diets, the rate of gallstone formation was reduced to 16.7% and 52,9% respectively. The mechanism of gallstone formation was explored through observing the effect of animals diet change on gallstone formation and the effect of ascorbic acid on gallstone prevention. At the same time, the micro-structure observation and the component analysis of gallstone, the change of ultrastructure of the liver and gallbladder, and the quantitative analysis of conjugated bile acids of biles were condueted.

Angiography ; Aortic Valve Stenosis ; Heart Valve Prosthesis ; Humans ; Tomography

Retinoblastoma (RB) is the most common intraocular malignant tumor in children. With advanced clinical technologies there are more and more methods available to treat retinoblastoma, and make it is possible to delivery individualized protocol combined traditional treatments with modern regimen to patients now. In order to improve the survival rate and the life quality of RB patients in China, it is very important to make a suitable system of standardized therapy based on results from developed countries and health policies of our own country.

Objective To study the extraction process of Jiejiu Gankang granules by orthogonal test.Methods With the ethanol extraction rates of the medical materials like ginseng,radix salviae miltiorrhizae,the water extraction rates of the medical materials like hawthorn,and Tanshinone ⅡA as the parameters,the extract conditions of Jiejiu Gankang granules was optimized by orthogonal design.Results The optimal preparation process was as follows:the mixture of medical materials like ginseng and Schisandra chinensis was refluxed twice with total 10 times of 70% alcohol,2.0 hours for each time,and then the mixture of medical materials like hawthorn extracted twice with total 10 times of boiling water,2.0 hours for each time.Conclusion The optimal preparation process is reasonable and with high extraction rate of active components.

OBJECTIVETo explore different regulatory effects of electroacupuncture (EA) at the same acupoint on the heart and the stomach.

METHODSEighty male SD rats were randomly divided into eight groups equally, i.e., the Neiguan (PC6) group, the Quchi (LI11) group, the Tianshu (ST25) group, the Danzhong (RN17) group, the Zusanli (ST36) group, the Sanyinjiao (SP6) group, the Xinshu (BL15) group, and the Weishu (BL21) group, 10 in each group. The regulatory effects of EA (2/15 Hz, 3 mA, 2 min) at different acupoints on the gastric motility (intragastric pressure) and the cardiac function (the left ventricular pressure) were observed. The mean values of effect of two-min pre-EA and after-EA were compared.

RESULTSCompared with the pre-EA in the same group, the left ventricular pressure decreased in the Neiguan (PC6) group, the Quchi (LI11) group, the Danzhong (RN17) group, the Sanyinjiao (SP6) group, and the Xinshu (BL15) group (P <0.05). The intragastric pressure increased in the Quchi (LI11) group, the Zusanli (ST36) group, the Sanyinjiao (SP6) group, and the Xinshu (BL15) group, while it decreased in the Tianshu (ST25) group, the Danzhong (RN17) group, and the Weishu (BL21) group (P <0.05). The difference of the left ventricular pressure was sequenced from high to low as Xinshu (BL15) >Danzhong (RN17) >Neiguan (PC6) >Sanyinjiao (SP6) >Quchi (LI11). The difference of the intragastric pressure was sequenced from high to low as Tianshu (ST25) >Weishu (BL21) > Xinshu (BL15) > Danzhong (RN17) > Zusanli (ST36) > Sanyinjiao (SP6) > Quchi (LI11).

CONCLUSIONSEA at the same acupoint (2/15 Hz, 3 mA) showed different regulatory effects on the heart and stomach. There existed some difference in the direction and size due to the acupoint location, meridians, dominant nerve segment or relating organs. A fixed effect direction to the specific target was also shown.

Acupuncture Points ; Animals ; Electroacupuncture ; Heart ; physiology ; Male ; Meridians ; Rats, Wistar ; Stomach ; physiology

Rats were immersed into boiling water for 15 seconds with scalded area about 23 cm~2 and decapitated at 0.5,1 and 6 hours after scalding. The changes of immunoreactive glueocorticoid receptor (GR_(IR)) and glucocorticoid receptor binding (GR_B) of hepatic cytosol after scalding were studied by both monoclonal antibody method and radioligand binding assay. The results showed that GR_(IR) and GR_B decreased markedly in parallel manner after scalding. Comparing with the controls (100%), the values of GRIR of 0.5,1 and 6 hours after scalding decreased to 64.76?13.320% (n=4),59.55?13.18% (n=5) and 42.64?4.24% (n=4)respectively; and relevent GR_B decreased to 50.24?9.71% (n=4), 51.11?11.31% (n=5) and 30.43?3.03% (n=4). The amplitude of decrease of GR_B was about 10% more than that of GR_(IR). Thus it might be concluded that the reduction of GR_B after stress was mainly due to the decrease of GR protein molecules but 10% of that was due to the change of GR binding activity. The mechanisms of these changes were discussed.

OBJECTIVE:To establish a HPLC method for the determination of 5-fluorouracil in human plasma METHODS:The plasma samples were extracted with ethyl acetate-isopropanol(85∶15,V/V)following precipitation of plasma proteins with ammonium sulfate The solvent was evaporated to dryness under a stream of nitrogen The dry extract was dissolved in mobile phase and then injected into the Diamonsil C18 column(150mm?4 6mm,5?m)and 0 01mol/L KH2 PO4(pH5 5) was taken as mobile phase The flow rate was 1 5ml/min,UV detection wavelength was 267nm The internal standard was 5-bromouracil RESULTS:The minimal detectable drug concentration in plasma was 0 025?g/ml The calibration curve was linear over a range from 0 3?g/ml to 10?g/ml The intra-day RSD≤5 0%(n=5) and the inter-day RSD≤11 5%(n=5) CONCLUSION:The method can be used for studying the pharmacokinetics and bioavailability of 5-fluorouracil

【Objective】To determine the content and dissolution of two imported levothyroxine sodium tablets.【Method】The content of levothyroxine sodium was assayed on a Hypersil BDS C18 column with a mobile phase consisting of methanol-0.85% phosphoric acid (60∶40) at a flow rate of 1.5 mL/min and detected at 225 nm.The study on dissolution was conducted according to the method Ⅱ of dissolution apparatus in USP(23).【Results】The average content of two formulations was 107.74% and 99.72%,respectively.The value of T50,Td and m of two tablets differed significantly (P<0.01).【Conclusion】There was a significant dissolution difference between two levothyroxine sodium tablets.

Described in this paper are the contents of book review and the success of book review achieved in Li-brary of Chinese Academy of Medical Sciences, such the expanded cooperative scope of book review, the extended medical fields involved in book review, the displayed academic value of book review in scientific research and teach-ing, the taken measures for spreading book review, and the forecasted future development direction of book review.

As the necessary depletion material in hospitals,sterile packing material shows category diversification when being selected.This paper briefly analyzes the functions and values of domestic common sterile packing material such as pure cotton wrap cloth,hard quality packing container,paper/ plastics packing bag and the crease wrapping paper.Reasonable suggestions are also put forward.