Chemotherapy -induced peripheral neuropathy ( CIPN ) is one of the most common adverse effects in chemotherapy .The mechanism of CIPN has always been attracting researchers′attention.Recently,the intimate relationship between nerve growth factor and CIPN is one of the most hot topics .NGF protects neurons from damage of chemotherapy drugs through inhibiting apoptotsis and other pathway to relieve the neurotoxicity . However ,there are still many problems in the clinical application of exogenous NGF .To improve the mechanism of NGF in the development and application approach of CIPN ,treatment of chemotherapy -induced peripheral neu-rotoxicity is of great significance .

Objective To investigate the protective effects of Xuebijing(XBJ)on the injury of vascular endothelial cells(VEC)induced by lipopolysaccharide (LPS),and to study the mechanisms of the production of nitric oxide (NO)and the expressions of inducible nitric oxide sytnhase (iNOS)and signal transduction under XBJ intervention condition.Methods The cultured VEC were divided into control group, LPS (1 mg · L-1 )group, LPS (1 mg·L-1)+XBJ(25 g·L-1)group,LPS(1 mg·L-1)+pyrrolidine dithiocarbamate(PDTC,20μmol·L-1) group;XBJ and PDTC were administrated 1 h before incubation of with LPS.Western blotting method was used to detect the expressions of iNOS and NF-κB p65 protein.The level of NO in the supernatant was measured by Griess reagent.Results Comparaed with control group,the NO level and the expression levels of iNOS protein and NF-κB p65 protein in VEC in LPS group were significantly increased(P<0.01).Compared with LPS group,the NO level and the expression levels of iNOS protein and NF-κB p65 protein in VEC in LPS+XBJ group were significantly decreased (P<0.05 or P<0.01);the NO level and the expression levels of NF-κB p65 protein and iNOS protein in VEC in LPS+PDTC group were significantly decreased(P<0.05 or P<0.01).There were no significant differences of the NO levels and the expression levels of iNOS protein between LPS+XBJ group and LPS +PDTC group (P>0.05),but the expression level of NF-κB p65 protein in LPS+PDTC group was lower than that in LPS+XBJ group(P<0.05).Conclusion XBJ can inhibit the production of NO and the expression of iNOS protein in VEC;its mechanism may be related to inhibiting the activation of NF-κB to control inflammation.

OBJECTIVE:To investigate the anti-angiogenic activity of ray cartilage glycosaminoglycans(RCG).METHODS:Primary culture of human umbilical vein endothelial cells(HUVEC)was performed,and MTT was adopted to evaluate the effects of RCG on the proliferation of HUVEC;Corneal neovascularization(CNV)was induced by sutures on Wistar rats,and CNV area was calculated to evaluate the effects of different concentration of RCG on CNV in Wistar rats.The model of mice with Lewis lung carcinoma was made to observe the effects of different concentration of RCG on tumor growth,with inhibition ratio of primary tumor measured and microvessel density(MVD)quantitated by immunohistochemical staining.RESULTS:RCG obviously inhibited the proliferation of HUVEC in vitro,with IC50 value at 62.93 mg?mL-1.As compared with normal saline group,RCG groups showed smaller areas of new blood vessels(P

Objective To investigate the relationship between the content of ET-1, CGRP, NO in myocardium and cardiac function in chronic renal ischemia of rats caused by abdominal aortic banding. Methods Male wistar rats (weight 180 - 200g) were randomly divid-ed into 2 groups, operation group (n=30) and sham operation group (n=10). Abdominal aorta ligation between right and left renal ar-tery was made with silk suture in operation group, and the narrow degree of aorta was about 50% which was controlled by ligateing with a syr-inge needle (7#). The aorta was not ligated in sham operation group. After 16 weeks of operation, invasive measurement of blood pressure and cardiac function were performed, and content of ET-1, CGRP and NO in myocardium were determined. Results Compared with sham operation group, the blood pressures of rats in operation group were significantly elevated, with cardiac systolic and diastolic function de-creased and left ventricular mass index increased. After 16 weeks, compared with sham operation group, the content of ET-1 in cardiac tissue were significantly elevated in operation group (P<0.01), while the content of CGRP (P<0.01)and NO (P>0.05)were decreased. There were negative correlation between the content of ET-1 in cardiac tissue and LV +dp/dt max(r = -0.37, P<0. 05). Conclusions In the state of chronic kidney iachemia caused by abdominal aorta ligation, content of ET-1 in cardiac tissues were increased while CGRP and NO were decreased. There were negative correlation between the content of ET-1 in cardiac tissues and LV systolic function.

Objective To investigate the expression changes of acetylcholinesterase (AChE) related microRNAs derived from peripheral blood mononuclear cells (PBMCs) in patients with stroke. Methods The microRNAs for targeting AChE mRNA were selected via prediction software and previous studies. PBMCs were extracted from venous blood samples of acute ischemic stroke patients (onset<24 h) and healthy controls. The expressions of microRNAs and AChE mRNA were quantified using real-time PCR (RT-PCR). The protein level of AChE was detected by Western blot assay. Results Thepredicted microRNAs included microRNA (miR)-24,-28,-124,-132,-182*,-194 and-484. The expression levels of miR-24,-124,-132 and-194 were significantly elevated in stroke patients compared with those of controls (P<0.05). There were no significant changes in expression levels of miR-28,-182*and-484. Additionally, the relative expression levels of intracellular AChE mRNA and protein decreased significantly in stroke patients (P<0.05). Conclusion MiRNAs can enhance cholinergic anti-inflammatory pathway by targeting AChE in patients with acute ischemic stroke.

Objective To compare the continuous subarachnoid block with ropivacaine or sufen?tanil either alone or in combination for labor analgesia. Methods Ninety nulliparous parturients who re?quired labor analgesia voluntarily, of American Society of Anesthesiologists physical status Ⅰ or Ⅱ, aged 23-35 yr, with a body height of 155-170 cm, were included in this study. When regular uterine contrac?tion appeared, labor analgesia was performed in the first stage of labor. The parturients were divided into 3 groups ( n=30 each ) using a random number table: ropivacaine group ( group R ) , sufentanil group (group S), and combination of ropivacaine and sufentanil group ( group RS). The spinal catheter was placed at L3,4 interspace. In group R, ropivacaine was given as an initial bolus of (0.6 mg∕ml) 5 ml fol?lowed by an infusion of 0.2 mg∕ml after the analgesia pump was connected. In group S, sufentanil was given as an initial bolus of (1.6 μg∕ml) 5 ml followed by an infusion of 0.2μg∕ml after the analgesia pump was connected. In group RS, the mixture of ropivacaine 0. 3 mg∕ml plus sufentanil 0. 8 μg∕ml was given as an initial bolus of 5 ml, followed by an infusion of the mixture of ropivacaine 0. 1 mg∕ml plus sufentanil 0.1 μg∕ml after the analgesia pump was connected. The analgesia pump was programmed to deliver a 5 ml bolus dose with a 15 min lockout interval, background infusion at a rate of 5 ml∕h, and the total volume of 100 ml in the three groups. The analgesia pump was connected at 30 min after the initial bolus was given, and the infusion was stopped at 2 h after delivery in the three groups. Visual analog scale ( VAS) scores were maintained ≤3. VAS scores were recorded before analgesia and at 5, 10 and 30 min after the initial bolus was given. The interval and duration of uterine contraction were recorded before analgesia, in 0-30 min, 30-60 min and 90-120 min of analgesia periods, and in the second stage of labor. The development of nausea and vomiting, pruritus, lateral episiotomy, assisted vaginal delivery, cesarean section, and post?dural puncture headache and requirement for oxytocin were recorded. Apgar scores at 1, 5 and 10 min after birth were recorded. Results The Apgar score of the newborn was more than or equal to 7 at 1, 5 and 10 min after birth in the three groups. Compared with the value before analgesia, the interval of uterine contraction was significantly prolonged, and the duration of uterine contraction was significantly shortened in the 0-30 min of analgesia period in group R ( P<0.05) , and no significant change was found in the inter?val and duration of uterine contraction in each analgesia period in S and RS groups ( P>0.05) . Compared with group R, the VAS scores were significantly increased at 5 and 10 min after the initial bolus was given, the interval of uterine contraction was significantly shortened, and the duration of uterine contraction was significantly prolonged in S and RS groups, and the incidence of pruritus was significantly decreased in group S ( P<0.05 or 0.01) . Compared with group S, the incidence of pruritus was significantly decreased ( P<0.01) , and no significant change was found in the VAS scores at each time point and interval and du?ration of uterine contraction in each analgesia period in group RS ( P>0.05) . Conclusion Continuous sub?arachnoid block with combination of ropivacaine and sufentanil provides better efficacy for labor analgesia than ei?ther alone.

STMN1 is a microtubule-destabilizing protein that regulates cell cycle by phosphorylation and dephosphorylation. It plays an important role in the proliferation and differentiation of cells, in addition to the tumorigenesis. This protein is highly expressed in a wide variety of human cancers, including leukemia and multiple types of solid tumors. The relationship between STMN1 and gastric cancer has recently been investigated. Studying STMN1 in gastric cancer is important. A number of studies have suggested that overex-pression of STMN1 can affect the therapeutic response of docetaxel, an anti-microtubule drug. This review summarizes the role of ST-MN1 in gastric carcinogenesis, development, prognosis, and treatment. The relationship between STMN1 and clinical pathology and its regulation pathways is also investigated.

Objective To study the mental hea lth of drug addicts and its correlation with addictive behavior. Method s 143 drug addicts in treatment were investigated and analyzed with The Symptom Checklist 90 and Addictive Behavior Questionnaire. Results The mental health of drug addicts was worse than that of normal peo ple, and somatization, depression, anxiety and psychopathy in the factor of SCL -90 had high proportion in high score field. Sex, having occupation or not, and living alone had no significant differences in SCL-90 score. The results of co rrelation analysis of mental health and addictive behavior were that the quantit y and frequency of drug use had positive correlation with mental health. The con trol power of drug use desire had negative correlation with mental health. Conclusion The mental health of drug addicts is worse, and addi ctive behavior is correlated with mental health.

Objective To compare the different effects of different titanic parameters on the expression of long-term potentiation (LTP) and to determine the optimal patterns of tetanizing stimulation in hippocampal CA1 area in adult male rats. Methods Four tetanic parameters of 100Hz were used in the experiment. Tetanus 1 was a short burst of stimulus composed of 60 pulses, which were divided into 5 sets, each set including 4-pulses-bursts. The inter-burst intervals are lengthened gradually, 1s, 5s, 30s, 10min in sequence. Tetanus 2 was a long burst of stimulus composed of 300 pulses in all, each burst including 20 pulses. The inter-burst interval is 2s, and tetanus 3 is also a short burst of stimulus composed of 15 bursts, each burst including 4 pulses. The inter-burst interval is 2s and tetanus 4 is a single train of burst composed of 60 pulses. The field excitatory post-synaptic potentials (fEPSPs) in hippocampal CA1 area were evoked by stimulating the Schaffer collateral fibers in the stratum radiatum and the expressions of LTP induced by the four parameters were evaluated. Results The amplitude and incidence of LTP induced by tetanus 1 were higher than those induced by the other three parameters. There was no statistical significance between the responses induced by the other three parameters. Both tetanus 1 and tetanus 3 could lead to long-lasting-LTP (LL-LTP, over 3h). Conclusion A high frequency stimulus with 200ms-inter-burst interval (theta frequency) is better in the induction of LTP in hippocampal CA1 area. A short burst of stimulus with 100Hz can evoke the expression of LL-LTP in hippocampal CA1 area.