AIM: To investigate the effects of c-fos antisense oligoneuleotide and p21 genetic transfection on the intimal proliferation of venous autografts. METHODS: The external jugule veins were autografted into common carotid arteries in the same side in 20 New Zealand rabbits, which were divided evenly into experimental and control group randomly. The transplanted veins of experimental group were immersed in the adenovirus - mediated p21 gene solution for 15 minutes just before anastomosis and coated with c-fos antisense oligoneucleotide glue gel just after anastomosis, while the control was only treated with empty vector. The transplanted vascular sample were taken at 2 weeks after operation. The intimal thickness (IT), degree of restenosis (DR), expression of proliferating cell nuclear antigen (PCNA), quantity of VSMC were determined by immunohischemistry. RESULTS: The IT , DR and expression of PCNA, VSMC were decreased, compared to control group. CONCLUSION: Transfection of c-fos antisense oligoneuleotide and p21 gene inhibits the intimal proliferation of venous antografs. [

Acupuncture Points ; Acupuncture Therapy ; Adult ; Female ; Humans ; Male ; Middle Aged ; Shoulder Pain ; therapy ; Young Adult

The eukaryotic expression vector pREP-8-IL-2 or and pREP-8-IFN-? was injected i.p. into mice by calcium phosphate coprecipitation method and IL-2 gene or IFN-? gene was successfully transfected into peritoneal M?s whose expression products could enhance the cytotoxicity of M?s, which secrete some TNF, IL-1 and NO, activate the nonspecific immunity and inhibit the growth of tumor effectively. In particular, IL-2 gene in combination with IFN-? gene transfection were successfully transfected into peritoneal M?s whose high expression products not only significantly enhance the M?s cytotoxicity and made it secrete high level TNF, IL-1 and NO, but also activated the CTLs of spleen and initiated specific immunity and nonspecific immunity . This would produce synergic antitumor effects. The results showed that IL-2 and IFN-? gene transfection produced more antitumor effects than IL-2 or IFN-? gene transfection alone.

The studies of nutritional status and biochemical indices of obese children were taken in three elementary schools with different socioeconomic levels. Subjects including obese and overweight children were matched with normal weight children by the ratio of 1:1. The caloric intake of the obese children was obviously higher than that of the normal children. There were significant differences between the obese and the normal children in concentrations of Hb and serum total protein (p

Lycobetaine ( AT-1840 ) is an effective antitumor substance. In the present work it was demonstrated that in a dose of 40 pig/kg AT-1840 could decrease the percentage of G, cells, meanwhile it increased G2 + M cells remarkably by means of flow fluocytometry method, AT-1840 could also inhibit the progression of cells from G2 to G1 phase. 8 to 72 h after, the injection the mitotic index was decreased significantly and recovered gradually in 6 d. It could inhibit the prophase and metaphase greatly, but had no marked influence on anaphase & telophase.

Objective In order to optimize patient's medical treatment processes, and to improve the working efficiency of health care professionals and the quality of medical services. Methods The design and application of the electronic prescription module were introduced, and its advantages and existing problems were discussed. Results The electronic prescription module were applied generally. Orthodox patient's medical treatment processes were changed and the prescription management was normalized. Conclusion The electronic prescription module is one of the core components of the workstation system for the out-patient physicians. It optimizes the patient's medical treatment processes and improves the efficiency and the quality of medical services.

Neovascularization plays an important role in embyonic development and many diseases. VEGFs and angiopoietins are two known growth factor families that are specific to vascular endothelium. The action of angiopoietins is associated not only with angiogenesis but also with postnatal vasulogenesis. Thus there is a good prospective use for angiopoietins or their antagonists to promote or inhibit angiogenesis in clinical therapy.

Objective To explore the diagnosis and treatment of closed abdominal injuries in infants.Methods A retrospectively analysis of the clinical data of 84 infants with closed abdominal injury was made.Results Among the 84 cases, 60 cases had closed parenchymal visceral injury (32 cases of liver and 28 cases of spleen), 10 cases had small intestine perforation, and 14 cases had soft tissue contusion of abdominal wall. Non-operative treatment was used in 27 cases of liver injury and 22 cases of splenic injury, operative treatment in 5 cases of liver injury and 6 cases of splenic injury, and operative treatment for the 10 cases of other types of injury. All the 84 infant patients were cured.Conclusions In diagnosis and treatment of closed abdominal injuries in infants, abdominal centesis, type-B ultrasound and CT should be rationally chosen and applied in order to quickly determine location and extent of visceral injury; indications for operation should be under strict control. Non-operative treatment of parenchymal visceral injury of infants is proved to be safe and with a high cure rate.

The short stature homeobox-containing(SHOX) gene,located in the short-arm pseudoautosomal region (PAR1) of the sex chromosomes,is one of the recently discovered genes,which is related to short stature.Its encoded protein,as a transcription activator,plays an important role in the regulation of growth.It has now been confirmed that the human SHOX gene mutation can cause L?ri-Weill syndrome,Turner syndrome,idiopathic short stature growth and its related characteristic skeletal deformities.This review makes a summary about SHOX gene defects,its clinical phenotype and treatment.

BACKGROUND: The incidence of deep venous thrombosis of the lower extremity after spinal surgery remains controversial, and its prevention is still under discussion.OBJECTIVE: To summarize the progress of the prevention of the deep venous thrombosis after spinal surgery.METHODS: The domestic and foreign related literature about the deep vein thrombosis after the spinal surgery, which was filtrated from CNKI and PubMed databases, were searched for the articles concerning the prevention of the deep venous thrombosis after spinal surgery published from January 1993 to January 2016.Finally, 41 eligible articles were included, and the prevention methods, risk factors and incidence were summarized.RESULTS AND CONCLUSION: (1) With the technology and medical equipment development, more novel technologies and biomaterials are extensively applied in spinal surgery; meanwhile, complex and difficult surgeries,big surgical trauma and prolonged operation time all increase the risk of deep venous thrombosis of the lower extremity;thereafter, the incidence is on a rise. (2) The prevention methods of deep venous thrombosis have been improved, and the grading of prevention becomes more sufficient. (3) During venipuncture and injection, upper extremity should be selected firstly, so as to avoid damage to the lower extremity veins. (4) The following studies should concentrate on understanding the epidemic characteristics of deep venous thrombosis of the lower extremity after spinal surgery, and confirming the best prevention time, as well as effective drugs and physical measurements.