Objective To evaluate the feasibility of combined application of helicobacter pylori stool antigen(HpSA)-and traditional serology in epidemiologic study of helicobacter pylori(Hp) infection in children. Methods Hp serum IgG antibodies were examined among 480 healthy children. Heticobacter pylori stool antigen(HpSA) were measured among children who were seropositive. Hp status was defined as positive when stool antigen test was positive, Hp status was defined as negative when seronegative. A case - control study was conducted to identify risk factors of Hp infection in preschool children. Results Among 480 preschool children, 172 (35 8 %) were sero- positive, 64(13.3 %) with positive result of HpSA test were diagnosed as current Hp infection. Univariate factor analysis demonstrated that Hp infection clustered in families and Hp infection was often acquired in childhood by oral-oral and fecal - oral person to person transmission. Conclusions HpSA and serology screening are two nomnvasive tests, simple not costly, effective and accurate. The combination of these 2 noninvasive methods can be applied in epidemiologic study of Hp infection in childrenJAppl Clin Pediatr,2004,19(11):948-950

Based on the standard strain-Vibrio parahaemolyticus BJ1.1997, temperature (7?C～43?C) and salinity (0.5%～9.5% NaCl) which affected its growth status was studied with uniform design. The results showed that Logistic equation was optimal in primary models, the second was Gompertz equation, the last was Linear equation, so growth parameters can calculated from Logistic equation. The secondary model was developed by square root models, its r value was 0.9863, the lowest growth temperature was 9.0506?C and the highest growth salinity was 5.93% [the corresponding lowest growth water activity (Aw min) was 0.9227]. Through F test, residual analysis and evaluation by bias factor and accuracy factor, the model can exactly describe the relationship between the growth rate and combined effect of temperature and salinity (water activity).

Animals ; Humans ; Ischemic Preconditioning, Myocardial ; MicroRNAs ; Myocardial Ischemia ; genetics ; prevention & control

Objective To study the sleeping quality and correlative factors of key senior middle school students of Beijing. Methods With pittsburg sleeping quality index (PSQI) scale, 344 key senior middle school students participated and completed the questionnaire. Results Percentage of sleep disorder accounted for 24.4% in the senior middle school students;Sleeping time was shorter than 7 hours in total of 61% students. Compared with key class students, sleeping time was shorter(P

Child ; China ; Evidence-Based Medicine ; Humans ; Pediatrics

Adolescent ; Child ; Child, Preschool ; Female ; Heart Rate ; Humans ; Male ; Sleep Apnea Syndromes ; diagnosis ; physiopathology ; Sleep Arousal Disorders ; diagnosis ; physiopathology

Objective To investigate the application of delayed sternal closure in the process of open heart surgery.Methods Delayed sternal closure was performed from Mar.2000 to Jun.2005 in 11 patients(4 males and 7 females,aged 16-67 years with an average of 42 years) after cardiac surgery.The indication included: cardiac dilatation(4 cases),intractable arrhythmia(4 cases),continuous bleeding(2 cases) and severe pulmonary edema(1 case).During the open chest period,the wound was covered with 3-layers of latex temporarily,and the delayed sternal closure was performed when bleeding was controlled,heart size reduced,and hemodynamic condition became stable.Results Except two patients died of acute kidney failure during open chest period,delayed sternal closure was successfully carried out in 9 patients 8-43 hours(23.7?11.0) after cardiac surgery.Except one patient died of cardiac arrest 21 days after operation,the 8 others survived and were discharged from ICU 1-8 days(3.9?2.4) after sternal closure,and the wounds healed well.Follow-up for the 8 survivors revealed an improvement of NYHA class(class Ⅰfor 4 cases,class Ⅱfor 2 cases,and class Ⅲ for 2 cases).Conclusion The delayed sternal closure is an effective method in the treatment of postoperative cardiac compression,severe bleeding and arrhythmia,and it does not increase the incidence of complications such as sternal infection.

Objective: To explore the myocardial protection of induction and reperfusion with warm blood cardioplegia for hypoxic immature myocardium. Methods: A hypoxic model of neonatal rabbits was established in this study. Some indexes,such as myocardial energy metabolism, water content, SOD, MDA and myocardial ultrastucture, were observed after induction and reperfusion with warm blood cardioplegia, comparing to cold crystalloid cardioplegia. Results: Hypoxic neonatal rabbits in this study presented cyanotis, hypoxemia and higher ratio of RV/(LV+S) simulating the pathophysiological changes in cyanotic congenital heart disease. It could be built and repeated easily in experimental researches of immature myocardial protection. Myocardial ATP content was (13.09?1.50) ?mol/g in studying group versus (11.53?5.40)?mol/g in control group (P

Objective To evaluate the results of Four-Step methods in traditional Chinese medicine Course.Methods 40 five-academic-year clinical medical students and 40 seven-academicyear undergraduate students were randomized assigned into experimental group (n=40, 20 five-academicyear clinical medical students and 20 seven-academic-year students) and control group (n=40, 20 five-academic-year students and 20 seven-academic-year students).The control group received conventional teaching, while four-step teaching break the gap between Chinese medicine and western medicine, build the base, form the framework of Chinese medicine and extend knowledge was implemented in the experimental group.Through questionnaire score, group discussion, the final evaluation of the theory and clinical training evaluation, the learning of the experimental group students and the control group students was compared.SPSS 18.0 statistical software was used and t test was employed to make analysis of the relevant data.Results The questionnaire at the end of the semester showed that the average score of students in the experimental group (36.18 ± 0.96) was higher than that in the control group (26.62 ± 1.00), and the difference was statistically significant (P=0.017).In the discussion at the end of the semester, the experimental group paid more attention to the theory and technology of traditional Chinese medicine's learning persistence in future and its application in their professional practice.Scores in theory test were higher in seven-academic-year students in experimental group (88.40 ± 8.79) than in control group (82.15 ± 6.83) and the difference was statistically significant (P=0.017), but there was no difference in five-academic-year students between experimental group (75A0 ± 12.09) and control group (77.85 ± 11.99), which was not considered statistically significant different (P=0.524).In clinical practice, the rate of outstanding students in the experimental group of seven-academic-year was five percentage points higher than the control group, while the rate of outstanding students in the experimental group of five-academic-year was 10 percentage points higher than the control group.Conclusions Four step teaching is helpful to stimulating the learning enthusiasm of the students of traditional Chinese medicine, expanding students' vision of medical field, and promoting their construction of a more comprehensive medical knowledge framework.

Objective To investigate the catalysis of Co - Cu /SiO2 in oxidation of sodium percarbonate (SPC ) for o-nitrochlorobenzene. Methods The solution of o-nitrochlorobenzene was prepared, acetic acid was used to adjust acidity, sodium percarbonate was applied as the oxidant, the self-prepared Co-Cu/SiO2 was used as the catalyzer to catalyze the degradation reaction of o-nitrochlorobenzene and the effect of reaction condition on degradation was researched. Results In the condition of loading amount of metal was 5%, nCo:nCu= 4:1,nCo-Cu=1.90?10-3mol,nSPC=6.36?10-3 mol,cOCNB=50 mg/L and t=80 ℃,pH=4,the degradation efficiency of o-nitrochlorobenzen could reach 92% . Conclusion Co-Cu/SiO2 is efficient in catalyzing the oxidative degradation of sodium percarbonate for o-nitrochlorobenzene and is applicable to the waste water treatment for eliminating o-nitrochlorobenzene