With the development of genomics , bioinformation , engineering , computer science and other fields, it has witnessed explosive growth of molecular diagnostic technologies.More and more technologies are used in desease diagnosis , therapy and prevention , which have presented a huge opportunity and challenge for clinical laboratory.Each technology has corresponding field of application , so it is a crucial problem for clinical laboratory to select appropriate molecular diagnostic technology for personalized medicine.

Lung cancer is one of the malignant tumors with a high morbitity and mortality in the world.During its neoplastic development, a set of changes in protein expression occurs.The changed proteomic profile of cancers—tumor biomarkers could guide clinical decision-making.Proteomic methods are widely used in identification of tumor biomarkers.To reduce the mortality of lung cancer and improve its therapy,many studies on the identification of this tumor biomarker are being carried out.This review summarizes the main strategies and technology in proteomics and its application in identification of lung cancer biomarkers.

Objective To explore the roles of executive function deficits in adolescent violent behaviors. MethodsAccording to the findings of general state questionnaire,modified overt aggression scale and interview,93 male juvenile delinquents were randomly divided into violent antisocial group(n=47) and nonviolent antisocial group(n=46).Besides,50 male adolescents in vocational schools were served as normal controls.All the participants were subjected to Wechsler adult intelligence scale(WAIS) test,Wisconsin card sorting test(WCST),Stroop color-word test(CWT) and Tower of Hanoi(TOH) test. Results There was no significant difference in IQ between violent antisocial group and nonviolent antisocial group(P0.05).In CWT,violent antisocial group and nonviolent antisocial group had significant difference with normal control group in number of correct responses of Stroop interference effects(SIE)(P0.05).In TOH,the total time spent in violent antisocial group and nonviolent antisocial group was significantly longer than normal control group(P0.05). Conclusion There exists executive function deficits in male adolescents with unlawful acts,and violent behaviors may have a closer relationship with executive function deficits.

Catheter Ablation ; adverse effects ; Heart Septum ; surgery ; Humans

Objective To survey the value of quantitative detection of urinary endotoxin for the diagnosis of gram-negative bacteria infection in urinary system,in order to provide evidences for early diagnosis and treatment.Methods The levels of urinary endotoxin were detected by kinetic turbidimetric technique,at the same time urine was cultured and WBC,CRP were detected.Results In 379 patients with gram-negative bacteria infection in urinary system,132 patients were upper urinary tract infection and 247 were lower urinary tract infection.The level of WBC,CRP and endotoxin were 11.25(7.98,13.99)× 109/L,81.36(76.64,83.50)mg/L and 49.57(17.28,78.37)pg/ml in upper urinary tract infection patients and in lower uri-nary tract infection patients were 10.24(7.94,12.24)×109/L,70.46(70.46,77.32)mg/L and 21.18(21.18,37.31)pg/ml. The group of upper urinary tract infection was higher than lower urinary tract infection on CRP and endotoxin,and have sig-nificant difference between the two groups of patients (P<0.05).Analysis of ROC,the diagnostic Cut-off value of 39.7pg/ml,the sensibility and specificity of endotoxin for the diagnosis of gram-negative bacteria infection on upper or lower urinary system were 84.1% and 90.3%.Conclusion The levels of urinary endotoxin detected by kinetic turbidimetric technique is simple and efficient,and have significant diagnosis value of gram-negative bacteria infection on upper or lower urinary sys-tem.

Objective The purpose of the present study was to examine the reliability and validity of the Chinese version of the impulsive-premeditated aggression scale (IPAS) in a sample of Chinese adolescents with aggression. Methods 126 adolescents completed the IPAS,Barratt impulsive scale and self-report questionnaires. In order to assess test-retest reliability, the IPAS was re-administered to 30 participants 3 weeks later.Results Item analysis showed that IPAS had satisfactory item discrimination, 6 items were excluded in the later analysis. The internal consistency of the IPAS Cronbach alpha was 0.77 and the test-retest reliability was 0.74.Exploratory factor analysis demonstrated two stable factors of aggression with good internal consistency and construct validity, the value of KMO was 0.646, the χ2 value of Bartlett test was 691.93 ( P <0.001). The confirmatory factor analysis showed that the value of χ2/df,GFI,AGFI,RMSEA were 1.42,0.874,0.837 and 0.058 respectively.The whole scale and the two secondly scales all correlated with BIS-11 and MOAS.Conclusion The results of the current study indicate that the Chinese version of the IPAS is a useful tool to subtyping aggressive behavior among adolescents, but it still need to be modified to apply in China.

Objective To study the changes rule of serum procalcitonin (PCT) and PCT clearance rate (PCTc) in patients with severe craniocerebral injury complicated with pulmonary infection and analyze the values of PCT and PCTc in the patients' therapeutic effect and prognosis.Methods A retrospective controlled analysis was conducted, the clinical data of 58 patients with severe craniocerebral injury complicated with pulmonary infection admitted into the Department of Neurology of the Forth Hospital of Tianjin from December 2012 to December 2016 were collected, and within 24 hours after definite diagnosis (before treatment) and after treatment for 1, 3, 7 days, the levels of PCT and percentages of PCTc were measured by rapid semi quantitative method. According to the therapeutic results of treatments, including antibiotics, airway management, nutritional support, etc., the patients were divided into an effective group and a deteriorative group. The changes rule of levels of serum PCT and percentages of PCTc before treatment and 1, 3, 7 days after treatment were dynamically evaluated. The receiver operating characteristic curve (ROC curve) was drawn and the area under the ROC curve (AUC) was calculated, and the sensitivity, specificity and diagnostic accordance rate of the combined treatment were assessed.Results Of 58 cases 32 (55.2%) after active treatment had promising therapeutic results or better prognosis (effective group), and the disease situations in 26 patients (44.8%) were deteriorating(deteriorative group). With prolonged treatment in effective group, the serum level of PCT was decreased gradually, while the deteriorative group showed a tendency firstly decreased and then increased, reaching to the valley on the 1st day after treatment, on the 3rd day beginning to increase and reaching the peak on the 7th day; the levels of PCT in effective group were significantly lower than those in deteriorative group at each time point after treatment on 1, 3, 7 days (μg/L: 1 day was 2.67±0.77 vs. 3.24±0.90, 3 days was 1.38±0.63 vs. 3.31±0.85, 7 days was 0.61±0.16 vs. 3.82±1.05, allP < 0.05); The PCTc rates in the effective group were significantly higher than those in the deteriorative group at each time point on 1, 3 and 7 d [1 day was (17.53±9.94)% vs. (12.31±7.55)%, 3 days was (60.34±13.55)% vs. (8.31±4.69)%, 7 days was (82.47±15.11)% vs. (-5.82±9.60)%, allP < 0.05]. After treatment for 3 days, 7 days, the AUC of PCT were 0.532 and 0.688, and the diagnosis cut-off values were 2.42 and 1.86 respectively; on 1, 3, 7 days after treatment, the AUC of PCTc were 0.529, 0.547, 0.775, and the diagnostic cut-off values were 15.67, 35.94 and 70.55 respectively. Further the final outcome was recognized as the gold standard: after treatment for 7 days, the PCT sensitivity was 80.1%, specificity 75%, the coincidence rate 77.6%; 7 days after treatment, the sensitivity of PCTc was 92.3%, the specificity 87.5%, the coincidencerate 89.7%.Conclusion The dynamic observations on serum PCT and PCTc have relatively good evaluation values on the therapeutic effect and prognosis of patients with severe craniocerebral injury complicated with pulmonary infection.

Objective To analyze the clinical characteristics, laboratory results, treatment and prognosis of adult onset Still's disease (AOSD). Methods The clinical and laboratory data of 107 AOSD patients were retrospectively analyzed. SPSS 22.0 was used for statistical analysis. Categorical data were described as frequency and percentage, and numerical data were described using x±s deviation. Chi-square test was used to compare categorical data between groups and analysis of variance test was used to compare numerical data between groups. K-W test was used to compare skewed distributed data between groups and J-T test was compared in pairwise comparison. Logistic regression was applied to examine prognostic factors. Results The average age of 107 patients (12 male and 95 female) was(39±16) years and the average disease course was (1.4±2.7) years. The patients were classified into 3 types based on their disease course: monocyclic (38 cases, 35.5%), polycyclic (29 cases, 27.1%) and chronic arthritis (40 cases, 37.4%). The clinical characteristics were high fever (107 cases , 100%), rash (97 cases, 90.7%) and arthralgia/arthritis (86 cases, 80.4%). The most common involved joints were knee (24 cases, 23.3%), wrist (19 cases, 18.4%) and proximal interphalangeal joints (14, 13.7%). The laboratory results were elevated including white blood cells count (88/105, 83.8%) and elevated serum ferritin (107, 100%). Seventy-three out of 84 patients (86.9%) had negative rheumatic factor and 9 out of 91 (9.9%) had positive ANA, which were low titre in general. Eleven patients (10.3%) had joint erosions suggested by X-ray at diagnosis. Intergroup analysis revealed that patients with polycyclic type were less likely to have abnormal liver function tests at disease onset (χ2=9, P<0.05). Twenty-five patients (23.8%) in total had complications, which were mainly pulmonary interstitial fibrosis (9 cases ) and hemophagocytic syndrome (7 cases). Male patients (n=6, χ2=5.93, P=0.015) were more likely to have complications. One hundred and two (95.3%) patients were treated with glucocorticoid and among them, 86 (84.3%) were combined with immunosuppressive agents. After 4 weeks of treatment, 89 patients (89.2%) achieved complete remission. The number of patients with glucocorticoid dependence or treatment failure was less in monocyclic group, although the differences were not significant. The analysis of prognostic factors founded that patients with Tmax>39.5 ℃ (P=0.030) were mostly fell into the monocyclic group. Compared with monocyclic type, patients with polycyclic or chronic arthritis type were less likely to have thrombocytosis (P=0.019, P=0.004). However, when compared with chronic arthritis type, patients with monocyclic or poly - cyclic type were less likely to have arthralgia/arthritis (P=0.030, P=0.000), especially the involvement of large joints (P<0.05). Conclusion AOSD mainly presents as high fever, rash, arthralgia and elevated inflammatory markers. Joint erosion may occur during the course of disease. The treatment of glucocorticoid and immunosup - pressive agent combinations is effective and most patients could achieve remission during 4 weeks of treatment. Patients with thrombocytosis at disease onset might be monocyclic type, while patients with arthritis, especially involvement of large joints at disease onset might be chronic arthritis type.

Objective To investigate the changes in tidal breathing lung function of severe pneumonia in re-covery phase and correlation with the condition and prognosis in infants and the young children.Methods Tidal breathing lung function of 44 cases of severe pneumonia infants and 50 cases of mild pneumonia infants in their recovery phase were measured by infantile spirometer made in Germany.According to ratio of exhaled volume at peak tidal expi-ratory flow to total expiratory volume(VPTEF /VE),severe pneumonia infants were divided into normal,mild,moderate and severe groups.All these children were followed up for the frequency of rehospitalization because of lower respiratory tract infections and the frequency of wheezing within 1 year after discharge.Statistical were analyzed by using t test andχ2 test.Results Compared with the mild pneumonia infants,the respiratory rate(RR)of the severe pneumonia infants was significantly increased [(36.31 ±0.78)times/min vs (30.83 ±0.74)times/min](P <0.01 );inspiratory time to expiratory time ratio(TI /TE)(0.65 ±0.01 vs 0.71 ±0.01 ),exhaled time at peak tidal expiratory flow(TPTEF) [(0.24 ±0.08)s vs (0.31 ±0.04)s]were significantly decreased (all P <0.01 );ratio of TPTEF to expiratory time (TPTEF /TE)[(1 9.1 5 ±0.77)% vs (23.00 ±0.51 )%],VPTEF /VE[(21 .28 ±0.86)vs (25.42 ±0.56)%]were significantly decreased (all P <0.01 ).The frequency of rehospitalization because of lower respiratory tract infections (χ2 =30.50,P <0.01 )and the frequency of wheezing within 1 year after discharge(χ2 =23.43,P <0.01 )in the severe pneumonia group were significantly higher than those of the mild pneumonia group;in the groups with different lung function damage by severe pneumonia,the frequency of rehospitalization because of lower respiratory tract infections (χ2 =1 0.64,P =0.033;χ2 =20.81 ,P =0.001 )and the frequency of wheezing (χ2 =1 0.53,P =0.037;χ2 =21 .25, P =0.001 )within 1 year after discharge in mild and severe group were significantly higher than those of the mild group,while there was no significant difference between normal group and mild group (P >0.05).Thirteen cases of se-vere pneumonia infants were followed up for 1 year.Of all these 1 3 cases,pulmonary function in 1 case restored to nor-mal within 3 months;that of 6 cases restored to normal within 6 months;the conditions of 3 cases restored to normal within 1 year;while the pulmonary function of other 3 cases was still abnormal 1 year after discharge.Conclusions Lung function damage of severe pneumonia infants is more serious than the mild pneumonia in infants in their recovery phase.For severe pneumonia infants,the more serious of lung function damages,the more likely to be re -hospitaliza-tion because of lower respiratory tract infections and wheezing in short time.

Objective To investigate the protective effect of hydrogen-rich saline on lung injury associated with severe acute pancreatitis and its impact on P38MAPK and NF-κB expressions.Methods Fifty-four male Wistar rats were randomly (random number) divided into three groups:(1) hydrogen-rich saline treatment group (HRS group,n =18),in which the rats were treated with hydrogen-rich saline (6 mL/kg) administered intravenously via tail vein and HRS (20 mL/kg) administered subcutaneously at 5 min after successful modeling.(2) Severe acute pancreatitis model group (SAP group,n =18),in which rats received equivalent volume of normal saline instead of hydrogen-rich saline both intravenously and subcutaneously as in HRS group.(3) Sham operation group (SO group,n =18),in which rats were treated with sham surgery,and received equivalent volume of normal saline as in SAP group.The model of severe acute pancreatitis (SAP) was made by retrograde injection of 5％ sodium taurocholate (1 mL/kg) into cholepancreatic duct.All rats were sacrificed at 3 h,12 h,and 24 h separately after the operation (n =6 at a time).The levels of serum amylase,lipase were measured.The ratio of wet and dry lung tissues was measured.The histopathological changes of lung tissues were observed under optic microscope.The expressions of P38MAPK,p-P38MAPK and NF-κB were measured by using immunohistochemistry method.Results Compared with SAP group,there were no significant differences in levels of serum amylase [12 h (5306.7±909) vs.(5435.0 ±441.2)] and lipase [12 h (1897.8 ±149.4) vs.(1917.9± 106.8)] in HRS group (P ＞0.05),but there were significant differences in the ratio of wet and dry lung tissues [12 h (3.12 ± 0.58) vs.(1.87 ± 0.25)] and histopathology scores [12 h (2.14 ± 0.38) vs.(3.58 ±0.32)] (P ＜0.05).There was no significant difference in expression of P38MAPK in lung tissues among three groups at 12 h.Compared with SO group,the expressions of p-P38MAPK and NF-κB were significant increased in SAP group at 12 h,however,they were lower significantly in HRS group than those in SAP group.Conclusions Hydrogen-rich saline has a protective effect on lung injury associated with severe acute pancreatitis,and its mechanism may be likely related to the antioxidant effect and inhibiting the activation of P38MAPK and NF-κB.