Objective The aim of this study was to explore the establishment method of an animal model of irritable bowel syndrome ( IBS) and the evaluation of this animal model.Methods 30 adult SD rats were randomly divided into two groups: acetic acid irritation and bondage stress group ( n=10 ) , bondage stress group ( n =10 ) , and normal control group ( n=10 ) .The rats of the intervention group received an intra-colonic infusion of 0.4% acetic acid irritation combined with bondage stress to establish an animal model of IBS.The colonic sensitivity of the intervention group rats was assessed by stool test and colorectal distension ( CRD) test.Hydrochloric acid toluidine blue staining was used to observe the number degranulation phenomenon of mast cells in the ileocecum.Results On the 7th day, the number of soft feces was 8 and loose stool was 4 in the model group, significantly higher than that in the bondage stress group(0 and 0) (P<0.05),and normal control group (1 and 0) (P<0.05).On the 10th day, when the AWR=2, the average rectal distension volume was 1.2 mL, significantly lower than that in the bondage stress group(1.37mL) (P <0.05),also significantly lower than in the normal control group (1.49 mL) (P<0.05), and when the AWR=4, the average rectal distension volume was 1.49 mL, significantly lower than that in the bondage stress group(1.74mL) (P<0.05),and the normal control group (1.77 mL) (P<0.05).These results indicated that the visceral sensitivity of the model group was significantly higher than that in the bondage stress group and normal control group.Histological analysis showed that the rats of all groups had no obvious inflammatory changes.Conclusions Chronic bondage stress combined with intra-colonic infusion of 0.4%acetic acid irritation can be used to increase the visceral sensitivity and amount and degranulation of mast cells in the intestinal tissue in rats.This established rat model shows pathogenetic changes resembling the pathogenesis of human irritable bowel syndrome, and provides a useful animal model for further studies of the pathogenesis of this disease.

[Objective] Probing into the effect of Changyanning syrup about colonic electrophysiology and motility of visceral hypersensitivity rat.[Methods] The test consists of the model series and the control series and the Changyanning syrup series,and the two latter rats were sensitized by intraperitoneal injection of chicken egg albumen.After two weeks,the stomach is poured 0.7ml's normal saline by blank space series,0.7ml's normal saline by model series,0.7ml's Changyanning syrup by Changyanning series.Continue to pour stomach in a month,writing colon fast wave,slow wave and the colon contraction,the observation of interdigestive myoelectric complex IMC cycle,Ⅲ times duration,fast wave and slow wave propagation rate,the largesttest amplitude,the amount of the colon contraction,the index of the colon contraction.[Result] The model series' interdigestive myoelectric complex IMC cycle prolongs,Ⅲ times duration prolongs,fast wave and slow wave propagation rate speeds up,the largesttest amplitude broadens.The slow wave propagation rate speeds up.The amount of the colon contraction speeds up,the index of the colon contraction speeds up.After Changyanning series treated,interdigestive myoelectric complex IMC cycle cuts down,Ⅲ times the duration cuts down,the largesttest amplitude decreases.[Conclusion] There are quite differencs between model group and control group in electricity and motion of visceral hypersensitivity rat.Changyanning syrup treating visceral hypersensitivity is by means of partly recovering the colon fast and slow wave and contraction wave rhythm and amplitude.

Objectire To investigate female urogenital fistula on the cause,diagnosis and treatment.Methods Thirty nine cases of patients were diagnosed ashaving genitourinary fistula by cystoscopy,intravenous urography,and blue methvlenelest.In 51%of cases were uretero-vaginal fistulas(20/39),one of them were trated byrenal ostomy.17 cases by early emergency ureteroneocystomy,2 cases by ureteralcatheter.In two complex patients with vesico.uretero-vaginal fistula(2/39),the repairwas associated with badder approach vesicovaginal fistula and early emergencyureteroneocystomy.In 44%of cases with vesicovaginal fistula(17/39)were trated bybladder approach after 3～27 months.Results 36 cases of patients were cured after surgery in one time,2 cases of ureteral intubation,1 case of nephrostomy and nephrectomy later with renal infection,1 case of uroclepsia complication.Conclusion Surgical repair of genitourinary fistula will have high rates of success if performed in proper time and appropriate approaches on the basis of patientinf eirmation and urinary fistula pathological type by experienced hands.

Objective To observe the clinical efficacy of hydroxyfasudil in senile heart failure caused by coronary heart disease (CHD). Methods 110 Patients with heart failure caused by CHD were randomly divided into two groups. 55 patients in control group were given conventional treatment, whereas the remaining patients were assigned into hydroxyfasudil group in the base of conventional treatment. After 6 months, we observed and compared the changes in cardiac function of the two groups. Results The effective rate of treatment group was 96.36% and the control group was 85.45%. There was statistical difference between the two groups (P < 0.05). Then we compared the degree of improvement of left ventricular diastolic function using ultrasonic cardiogram and Tei index. Left ventricular ejection fraction of treatment group improved significantly but biochemical indicators of two groups had no statistical difference. Conclusion In patients with heart failure caused by CHD, supplementation of hydroxyfasudil based on conventional therapy could improve diastolic function , potentiate left ventricular remodeling and enhance the life quality. Furthermore, it′s more sensitivity and reasonable to assess cardiac function using Tei index as compared with using ultrasonic cardiogram.

Both the concept and characteristics of materials circulation management in hospital are expounded.In order to implement materials circulation management in hospital,it is proposed that not only the outside resources should be utilized rationally,but the control of internal materials circulation links should be strengthened,and specially the purchasing and storage management should be highlighted.According to the experience of materials circulation management,it is very necessary to carry on internal materials circulation management in hospital.Any improvement in any link will play a driving role in the improvement of the management level of the hospital.At meantime,the scientific system of internal logistics management should be set up and improved constantly in hospital.

Objective The present study was undertaken to examine the regulatory effect of endogeous sulfur dioxide (SO2) on interleukins in rats with acute lung injury (ALI) induced by sepsis.Methods Twenty Two male Sprague Dawley (SD) rats were randomly (random number) divided into sham group (Control group),sham + SO2 donor (Na2SO3/NaHSO3,0.54 mmol/kg:0.18 mmol/kg) group (SO2 group),sepsis induced ALI group (sepsis group) and sepsis induced ALI with sulfur dioxide pretreatment group (sepsis + SO2 group).Index of quantitative assessment of histological lung injury (IQA),wet/dry weight ratio (W/D) and the level of SO2 in plasma were measured.IL 6,IL 8,IL 10,MCP-1 and TNF-α were measured by ELISA.Results The IQA score and W/D ratio of lung tissues significantly increased in sepsis rats compared with control group (all P ＜ 0.01),but significantly decreased in sulfur dioxide pretreatment rats compared with in sepsis rats (all P ＜0.01).The level of SO2 in plasma decreased in sepsis rats (3.17 ± 3.44) μmol/L compared with control group (5.87 ± 1.96) μmol/L,but significantly increased in sulfur dioxide pretreatment rats (9.78 ± 3.26) μmol/L compared with in sepsis rats (P ＜0.01).The level of IL 6,IL 8,IL 10,MCP-1 and TNF-α significantly increased in sepsis rats [(87.08 ± 22.03) pg/mg,(79.82 ± 19.69) pg/mg,(66.38 ± 21.77) pg/mg,(157.58 ± 42.36) pg/mg and (65.04 ± 19.42) pg/mg,respectively] compared with control group [(47.41±9.64) pg/mg,(42.25±8.16) pg/mg,(31.96±4.63) pg/mg,(67.65±10.18) pg/mg and (33.83 ± 5.75) pg/mg] (all P ＜ 0.01).but the level of IL 6,IL 8,IL 10 significantly decreased in sulfur dioxide pretreatment rats [(66.01 ± 16.52) pg/mg,(61.52 ± 18.32) pg/mg and (45.61 ± 16.47) pg/mg,respectively] compared with sepsis group (all P ＜ 0.05).The level of MCP-1 significantly decreased in sulfur dioxide pretreatment rats (117.86 ± 34.20) pg/mgcompared with sepsis group (all P ＜0.01).The level of TNF-α decreased in sulfur dioxide pretreatment rats (61.49 ± 15.33 pg/mg) compared with sepsis group.Conclusions These results suggested that exogenous SO2 could inhibit the pulmonary tissue inflammatory response by inhibiting MCP-1 and TNF-α in rats with sepsis induced ALI.

BACKGROUND:Spine infection models are rarely reported in the literature. There are fewer reports on the imaging and histological observations of the animal model of spinal infection.
OBJECTIVE:To develop a canine model of pyogenic spinal infection.
METHODS:Twelve Chinese dogs were adopted in the study. The dogs were in the right arm recumbent. The surgery was conducted by retroperitoneal approach to expose T12/L1 intervertebral space. The intervertebral discs that exposed the intervertebral space were partial y removed, and the end plate of the adjacent two vertebrae was deliberately curetted out. Suspension with different concentrations of Staphylococcus aureus and sodium morrhuate were injected into the dog intervertebral space. The aim of removal of partial intervertebral discs and destruction of endplate was to make the vertebral body bone better in contact with the bacteria. The same operation was conducted in L5/6 clearance of the animal spine. At 14 days after surgery, animals were sacrificed. The infected intervertebral disc and adjacent vertebral bodies were taken out. Bacterial contents of specimens were quantitatively determined. During sacrificing, partial liver tissues and blood samples were col ected for bacterial culture. The occurrence of systemic infection was observed.
RESULTS AND CONCLUSION:(1) Spondylodiscitis of the lumbar spinal column was consistently produced in 90%(9/10) the sites chal enged with 102 colony-forming units (CFU) Staphylococcus aureus. Liver biopsy and blood culture did not show any signs of systematic infections. (2) Two out of four animals implanted the suspension with a concentration higher than 103 CFUs died within 3 days post-implantation. (3) These results suggested that a new canine model of pyogenic spinal infection was developed. This model could be used to test the efficacy of different anti-infection strategies.

BACKGROUND:Mesenchymal stem cells are a kind of adult stem cells with the potentials of self-replicating and multi-directional differentiation. Under certain conditions, the cells can develop into bone, cartilage and other types of cells. Granulocyte colony-stimulating factors can promote regeneration of mesenchymal stem cells.
OBJECTIVE:To observe the osteogenic induction effect of granulocyte colony-stimulating factors by animal experiment, and provide a better treatment method for clinical treatment of bone defect.
METHODS:Forty New Zealand rabbits were randomly divided into treatment group and model group. Al animals were removed 2 cm length of the right radial bone and the surrounding periosteum, and then implanted with demineralized bone matrix into the defect. The treatment group was subcutaneously injected with granulocyte colony-stimulating factor 30μg/kg per day, the injection was started at preoperative 3 days and given for 7 continuous days. In control group, the same dose of physiological saline was injected.
RESULTS AND CONCLUSION:In the treatment group, the number of mesenchymal stem cells, the quantity and quality of bone formation at bone defects were better than those in the model group. The experimental findings indicate that granulocyte colony-stimulating factor can obviously increase the number of mesenchymal stem cells and promote bone formation at the area of bone defect, which can contribute to early repair of bone defects.

Objective To investigate the prevalence of cervical spondylosis among adults in Beijing.Methods The prevalence of cervical spondyiosis among people older than 18 years who had lived in Beijing for more than six months was investigated in December 2010.The multi-stage sampling was used in this cross-sectional study.The related information was obtained by a self-designed questionnaire.Single and muhivariable Logistic regression models were applied to analyze high risk districts and populations.Results A total of 3859 people were enrolled in this study.Among them there were 531 people with cervical spondylosis,and the prevalence was 13.76％.The prevalence of cervical spondylosis in suburb (15.97％) was the highest compared with city center and countrysides (x2=8.257,P=0.016).The prevalence in females (10.49％) was higher than that (16.51％) in males (x2=29.432,P＜0.001).The distribution of prevalence among different age populations was inverted U shape,and the prevalence was higher in 45 years group (20.43％) and 60 years group (18.91％) compared with other two groups.The prevalence in employed group was 1.303 times higher than that in unemployed group (95％CI:1.008,1.684).According to the muhivariable logistic regression result,suburb (OR=1.306,P=0.026),females (OR=1.81 1,P＜0.001),people who were 45years or 60 years (OR=2.171,P＜0.001; OR=2.430,P＜0.001,respectively) and employed and retired people (OR=1.664,P=0.001; OR=1.303,P=0.043,respectively) were high risk population of cervical spondylosis.Conclusion The prevalence of cervical spondylosis among adults in Beijing was much higher and vastly distributed.It's necessary to reinforce the prevention,diagnosis and treatment study.Young females,people aged 45 years or 60 years,and employed people were high risk populations.

Animals ; Colon ; physiology ; Female ; Indoles ; pharmacology ; Male ; Myoelectric Complex, Migrating ; Rats ; Rats, Wistar ; Viscera ; physiology