Objective To evaluate the frequency of the infections with Ureaplasma urealyticum and/or Mycoplasma honinis in preterm 23 to 32 week births and the association with the outcomes of new born. Methods One hundred and eighty-seven mother/premature infant dyads with deliveries between 23 and 32 weeks gestational age. Ueaplasma urealyticum, Mycoplasma honinis and IL-6 were determined by PCR and radioimmunity. Medical information and outcome of newborns were recorded. Results Compared with control group, the incidence of Ureaplasma urealyticum and/or Mycoplasma honinis, IL-6 and preterm premature rupture of the fetalmembranes ( PPROM ) were higher in spontaneous group. The PPROM were more common among infants with a positive Ureaplasma urealyticum and/or Mycoplasma honinis. Infants with positive cord blood Ureaplasma urealyticum and/or Mycoplasma honinis were likely to have neonatal systemic inflammatory response syndrome (SIRS) and bronchopulmonary dysplasia (BPD), but not significantly different for respiratory distress syndrome (RDS). Conclusion Infection with Ureaplasma urealyticum and/or Mycoplasma honinis may play an important role in the causal pathway for spontaneous preterm labor, and impact the outcome of premature infants.

Objective To investigate the changes in tidal breathing lung function of severe pneumonia in re-covery phase and correlation with the condition and prognosis in infants and the young children.Methods Tidal breathing lung function of 44 cases of severe pneumonia infants and 50 cases of mild pneumonia infants in their recovery phase were measured by infantile spirometer made in Germany.According to ratio of exhaled volume at peak tidal expi-ratory flow to total expiratory volume(VPTEF /VE),severe pneumonia infants were divided into normal,mild,moderate and severe groups.All these children were followed up for the frequency of rehospitalization because of lower respiratory tract infections and the frequency of wheezing within 1 year after discharge.Statistical were analyzed by using t test andχ2 test.Results Compared with the mild pneumonia infants,the respiratory rate(RR)of the severe pneumonia infants was significantly increased [(36.31 ±0.78)times/min vs (30.83 ±0.74)times/min](P <0.01 );inspiratory time to expiratory time ratio(TI /TE)(0.65 ±0.01 vs 0.71 ±0.01 ),exhaled time at peak tidal expiratory flow(TPTEF) [(0.24 ±0.08)s vs (0.31 ±0.04)s]were significantly decreased (all P <0.01 );ratio of TPTEF to expiratory time (TPTEF /TE)[(1 9.1 5 ±0.77)% vs (23.00 ±0.51 )%],VPTEF /VE[(21 .28 ±0.86)vs (25.42 ±0.56)%]were significantly decreased (all P <0.01 ).The frequency of rehospitalization because of lower respiratory tract infections (χ2 =30.50,P <0.01 )and the frequency of wheezing within 1 year after discharge(χ2 =23.43,P <0.01 )in the severe pneumonia group were significantly higher than those of the mild pneumonia group;in the groups with different lung function damage by severe pneumonia,the frequency of rehospitalization because of lower respiratory tract infections (χ2 =1 0.64,P =0.033;χ2 =20.81 ,P =0.001 )and the frequency of wheezing (χ2 =1 0.53,P =0.037;χ2 =21 .25, P =0.001 )within 1 year after discharge in mild and severe group were significantly higher than those of the mild group,while there was no significant difference between normal group and mild group (P >0.05).Thirteen cases of se-vere pneumonia infants were followed up for 1 year.Of all these 1 3 cases,pulmonary function in 1 case restored to nor-mal within 3 months;that of 6 cases restored to normal within 6 months;the conditions of 3 cases restored to normal within 1 year;while the pulmonary function of other 3 cases was still abnormal 1 year after discharge.Conclusions Lung function damage of severe pneumonia infants is more serious than the mild pneumonia in infants in their recovery phase.For severe pneumonia infants,the more serious of lung function damages,the more likely to be re -hospitaliza-tion because of lower respiratory tract infections and wheezing in short time.

Objective To observe the expressions of vascular endothelial growth factor(VEGF) and cyclooxygenase-2(COX-2) in the cerebral tissue following hypoxic-ischemic encephalopathy(HIE) in newborn rats and explore the machanism of inosine in protecting against hypoxic-ischemic brain damage(HIBD).Methods Sixty-six newborn rats aged 7 days were divided into sham,control and experimental groups.HIE models were made by clamping the right cervical artery and making hypoxia for 2 hours.The rats in experimental group began injecting inosine,at 1 day before experiment,and the rats in the sham and control groups saline solution with same dose.The samples were made at the given time,and expressions of VEGF and COX-2 were investigated by immunohistochemical technique.Results The cerebral tissue had no expression of VEGF and COX-2 in sham group.From 2 hours on cortex and striatum after HIE in control and experimental groups,expressions of VEGF and COX-2 increased rapidly,peaking at 12-24 hours,and then decreased gradually.Expressions of VEGF and COX-2 were higher in experimental group(All P

Objective To explore the clinical significance and changes of tumor necrosis factor-?(TNF-?)and interleukin-6(IL-6)level in children with acute viral myocarditis(AVM).Methods TNF-? and IL-6 level in serum from 53 AVM children and 20 healthy children were measured by radio-immunoassay and enzyme-linked immunosorbent assay(ELISA).The relation between the levels of TNF-? and IL-6 in serum with the occurrence and development in acute viral myocarditis was analyzed.Results Thirty-two were males and 21 females in the 53 AVM children.The ages ranged from 3-11 years old.There were 12 males and 8 females in control group.The serum level of TNF-? and IL-6 were(526.7?32.9),(3.23?0.53)mg/L in AVM group and(383.1?27.5),(1.63?0.22)mg/L in control group.There were significant difference between the 2 groups(Pa

Objective To discuss the frequency distribution of single nucleotide polymorphisms (SNP) of four asthma-related gene loci in asthmatic children of Henan,and to investigate its association with genetic susceptibility to childhood asthma and some clinical phenotypes of asthma Methods Fluorogenic quantitative PCR and sequencing technique were employed to detect the frequency distributions of the SNP of the four asthma-related gene loci in 135 asthmatic children and 98 healthy controls.Results The genotype DD of angiotensin-converting enzyme (ACE)had a significantly higher frequency in the asthmatic children than in the healthy controls (x2 =26.475,P ＜ 0.01),and the frequency of D allele was also significantly higher in the asthmatic children than in the healthy controls (x2 =24.242,P ＜0.01).The genotype AG of Adrenaline receptor beta 2 subtypes (ADRB2) had a significantly higher frequency in the asthmatic children than in the healthy controls (x2 =22.505,P ＜0.01),and the frequency of allele was also significantly higher in the asthmatic children than in the healthy controls (x2 =6.759,P ＜ 0.01).Conclusions Genotype DD of ACE and genotype AG of ADRB2 are related to genetic susceptibility to childhood asthma and may be the risk factor for childhood asthma of Henan.Another two asthma genes involved in this study are not be able to repeat.

Objective To construct series of reporter plasmids with truncated and deleted hTERT promoter.Methods Gene fragments of hTERT promoter was amplified by PCR and cloned into pGL3-Basic to construct luciferase reporter vectors.Dual luciferase assays were performed with cell lysates of HepG2 and COS-7 cells cotransfected with hTERT promoter reporter plasmids and pRL-TK.Results Series of luciferase reporter plasmids with truncated and deleted hTERT promoter were successfully constructed and respectively named pGL3B-895,pGL3B-371,pGL3B-DELS2,pGL3B-349,pGL3B-329,pGL3B-318,pGL3B-306.Dual luciferase reporter assays showed that all the reporter vectors have promoter activity both in HepG2 and COS-7.Conclusion Series of luciferase reporter plasmids with truncated and deleted hTERT promoter were successfully constructed,and their promoter activity were verified.These plasmids provide necessary experimental naterials for further investigation of regulation of hTERT during hepatocarcinoma development.

Objective To retrospectively review the efficacy and side effects of intense pulsed light (IPL) and red light emitting diode (LED) in the treatment of steroid-dependent dermatitis.Methods Seventy patients with steroid-dependent dermatitis mainly manifesting as facial telangiectasis were treated with IPL for an average of 3.49 sessions with a 4-week interval.The energy density of IPL varied from 20 to 23 J/cm2,pulse width from 2.6 to 5.0 ms,and delay from 15 to 20 ms.Meantime,197 patients with steroid-dependent dermatitis,who mainly presented with facial skin sensitivity,were treated with red LED (633 ± 3 nm wave length) twice a week for an average of 4.23 sessions.The energy density of red LED was 128 J/cm2,and the irradiation lasted 20 minutes at each treatment.The efficacy and adverse reactions were assessed and recorded for each treatment.Results The total response rate was 88.57％ for IPL,and 83.76％ for red LED.There was a significant difference in the clinical efficacy between triple-pulse and double-pulse IPL (x2 =8.14,P ＜ 0.05).No severe adverse reaction was observed in any of the patients.Conclusion IPL and red LED are both effective in treating steroid-dependent dermatitis.

Objective To study pathogen aetiology and distribution of drug resistance in neonatal septice-mia. Methods A retrospective study of positive organisms of blood cultures and the results of antimicrobial suscep-tibility testing was conducted from January 2000 to December 2006 in 1293 cases of neonatal septicemia;The results were divided into three groups:the earlier group,the middle group and the later group. The strains were identified by VITEK-AMS and antibiotics susceptibility was tested by K-B method, results were interpreted according to NCCLS. inant in three groups(76.3% ,74.1% and 65.7% ) (P <0.05). Among Gram-pesitive coccus,the most prevalent strains was staphylococcus, Gram-negative bacilli increased gradually,accounting for 21.3% ,21.3% and 28.4% in cpidermids to erythromycin and sulfamethoxazole/trimethoprim were rather higher:93.3% ,73.7% vs 87.8% vs 100.0%, respectively, the resistance -rates to penicillin were less,but to susceptible to vancomyein,piperacill/tazo-batam,amikacinand ciprofloxacin;gram-negative bacilli were only sensitible to imipenem, cefepime, and cefopera zone/sulbactam;multiresistances of drug strains was commonly seen. Condusions It is important for using antibi-otics rationally according to drug sensitivity test, because multiresistant strains and opportunistic pathogen are com-monly seen.

Objective To report the clinical, myopathological and genetic features in myofibrillar myopathy (MFM) with numerous cytoplasmatic-spheroid bodies. Methods Ten patients in 5 successive generations began to present progressive proximal limbs weakness at 35 to 40 years old. Additionally, 4 cases manifested diarrhea and 6 cases accompanied with cardiorespiratory symptoms. An open biopsy was performed on the proband. In addition to histological, enzymhistochemical staining and ultrastructural examination, immunohistochemical staining with antibody against tau, desmin, ubiquitin, dysferlin, dystrophin-C', dystrophin-N' and dystrophin-R were done. All the exons of the MYOT, CRYAB, DESMIN, LDB3, LMNA, SEPNI gene and the FLNC exon 48 were analysed. Results Cytoplasmatic bodies and spheroid bodies were found in the fibers. The deposited material were positive for tau, desmin, ubiquitin, dysferlin and dystrophin-R, dystrophin-C'. Electron microscope showed granular dense Z-disc material in the inclusions which were surrounded by thin filament. There was no mutation in the above exons of the 7 candidate genes. Conclusions Myofibrillar myopathy involves multiple system impairment. Cytoplasmatic and spheroid bodies contain microtubule and membrane associated protein. The disease might be induced by some unknown genetic abnormities.

Clinical teaching is an important link in cultivating clinician,Heuristic teaching can stimulate students'activeness of learning,enlighten their thoughts,arouse their positivity and creativity,find out their inner potential,and increase teaching effect.Intractable case discussion is an important method of heuristic clinical teaching,which can help strengthening theory knowledge,exercising correct clinical thinking,creating scrupulous scientism and satisfactory medical ethics.