Objective To investigate the pregnancy outcome of gestational intrahepatic cholestasis.Methods 70 pregnant women with intrahepatic cholestasis were selected as the study group.70 healthy pregnant women during the same period were selected as the control group.The pregnancy outcomes of two groups were compaind.Results (1) The maternal serum levels of bile acid,alanine aminotransferase,aspartate aminotransferase in study group were (65.61 ± 13.5) μmol/L,(134.31 ± 24.7) U/L,(97.35 ± 21.54) U/L,which were higher than those of the control group (3.34 ± 0.41) μ mol/L,(36.16 ± 4.15) U/L,(23.34 ± 4.45) U/L,and the differences were statistically significant(P ＜0.01).(2) In study group,the incidence rate of maternal gestational hypertension was 21.42％,premature rupture of membranes was 17.14％,incidence rate of postpartum hemorrhage was 15.71％,which were significantly higher than 7.14％,5.71％,4.29％ in the control group,the differences were statistically significant(P ＜ 0.05).(3) In study group,the incidence rate of neonatal asphyxia was 27.14％,therate of amniotic fluid contamination was 35.71％,fetal distress was 22.86％ and 30.00％ of the childrenwith low birth weight,whichwere higher than those of the control group,the differences were statistically significant(P ＜0.01).Conclusion Pre gnancy intrahepatic cholestasis can increase the incidence rate of perinatal complications,has serious impact on the prognosis of the fetus,and to strengthen the monitoring of pregnancy intrahepatic cholestasis has important clinical significance.

Objective To establish the relationship of serum TNF-? and IL-6 contents with myocardial cell apoptosis in the chronic heart failure (CHF)rats,and to explore the therapeutic mechanism of Anxin Granules (AXG)in treating CHF.Methods Fifty female SD rats were randomly divided into five groups:normal control group (A),model group (B),captopril group(C),low-dosage AXG group (D)and high-dosage AXG group (E).CHF models were established by intraperitoneal injection of small-dose of adriamycin,once per week for continuous 6 weeks.Meanwhile,group C was given captopril injection (6.25mgkg-1d-1),and groups D and E received gastric gavage of low-dosage AXG (0.8 gkg-1d-1)and high-dosage AXG (1.6gkg-1d-1)respectively.After 6 weeks of modeling,serum levels of TNF-? and IL-6 of different groups were tested by ELISA,the apoptosis of myocardial cells was tested by TUNEL,and then the apoptosis index(AI)was counted.Results Serum levels of TNF-? and IL-6 were increased in the model group (P

Objective To explore the pathogenic course,clinical features and prognosis on hallervorden spatz disease(HSD).Methods To review and sum up clinical data of three patients of 1985 with HSD in one family,and followed up survey in 2001.Results The same parents had three(2 males,1 female) of six children who were suffered from HSD.Clinical features include bilateral pigmentary degeneration of the retina,optic atrophy and progressive dementia,and lay in bed after several years with convulsive seizure,opisthotonus,limb rigidity,hyperreflexia, pyramidal sign positive;they were suited therapy to the illness.2 cases for death,another case for progressive deterioration,the patient lost labour ability and couldn't take care of himself.Conclusion HSD is kindred hereditary disorder,major cases occur the symptoms before 20 years old (progressive deterioration).After attacking about 20 years the patients died.The HSD gene located on chromosome 20p12.3 p13.

Objective To study the diagnostic evaluation of spiral CT for acute pulmonary embolism(PE).Methods 24 patients with PE were scanned with SCT(Philips AVEL.)pre-and post-injection of 100 ml contrast medium(Ultravist or Omnipaque 300 mgI/ml)from elbow vein.Enhanced CT scans were started after injection of 15 and 30 seconds respecting.Results The indirect signs on plain CT image were:lung-making sparse in 12 cases;lesion of pulmonary infarction in 11 cases;pulmonary hypertension signs in 3 cases;pleural thickening in 4 cases;pleural effusions in 8 cases.The direct signs on enhanced CT images were:Intraluminal filling defect (mural filling defect in 32 vessels;partial filling defect in 30 vessels;total occlusion in 92 vessels and central filling defect that was railway-track sign in 15 vessels)and smaller caliber of pulmonary artery in 14 vessels.Conclusion Enhanced pulmonary SCT angiography is the safe,quick and effective imagilogic examination for acute PE.

AIM: To investigate the effects of fluvastatin on the airway remodeling in a guinea pig model of asthma. METHODS: Asthmatic guinea pig model was established by intraabdominal injection of ovalbumin and aluminium hydroxide and challenged with ovalbumin once every other day for 60 days. 30 guinea pigs were randomly divided into three groups: control group ( n= 10), asthma group ( n= 10) and fluvastatin plus asthma group ( n= 10) in which fluvastatin was inhaled at concentration of 0.5 g/L 30 min before every challenging. The thickness of airway smooth muscle layers of every three groups were compared after Haematin-Eosin staining by image analysis system. The level of ras mRNA in airway were examined by Dot-blot molecular hybridization. The expression levels of ras p21 were also examined by immunohistochemical technique. RESULTS: The mean thickness of airway smooth muscle in asthma group was (74 27?3 30) micrometer, greater than that of control group [(38 57?3 37) micrometer ( P

Objective: To develop a way of high-quality real-time three dimension surface reconstruction for high-resolution volume data.Method 3D surface point sets of single organ were using a method of binding the threshold and morphological operations.The normal vector of every surface point was calculated.According to the gray gradients of volume data,the triangle face was replaced by surface points to describe the organ surface,and the surface was displayed with OpenGL interface of display card after defining the color and transparent of the organ surface.Result Based on hardware platform of personal computer,the reconstruction of skeleton and skin for the digitized virtual Chinese man No.1(VCH-M1) from CT database was constructed,the rendering speed was faster than 25 F/s.Conclusion The algorithm is capable of realizing a real-time rendering for 512?512?1720 high resolution volume data.

Objective To examine the localization of neurokinin B receptor (NK3)\|like immunoreactivity (\|LI) in the central nervous system of the mouse. Methods An immunohistochemcial staining method was used. Results NK3 receptor\|LI was localized in somatic and dendritic profiles in the most parts and in neuropil in a few regions of the mouse central nervous system. A large number of neurons with NK3\|LI was seen in the anterior olfactory nuclei, accumbens nucleus, septal area, ventral pallidum, pallidum, caudate putamen, nucleus of the stria terminalis, anterior hypothalamic area, tuber cincreum area, lateral hypothalamic area, perifornical nucleus, supraoptic nucleus, arcuate nucleus, mammillar nuclei, substatia nigra, ventral tegmental area, retrorubral area, superior and inferior colliculus, periaqueductal gray, nucleus of the solitary tract, and superficial layers of the medullary and spinal dorsal horns. The superfical layers of the cerebral cortex, piriform cortex, dorsal hippocampus, amygdal complex, reticular formation of the brainstem contained some neurons with NK3 receptor\|LI. In the ventral hippocampus, median and intralaminar nuclei of the thalamus and interpeduncular nuclei, NKR\|LI was localized in neuropil. Conclusion\ Neurons with NK3 receptor\|LI were widely distributed in the central nervous system. It may be involved in many physiological functions in the central nervous system of the mouse.\;[

Objective To investigate the effect of notch signaling pathway on drug resistance and invasiveness of bladder cancer .Methods We observed the changes of growth and morphology of bladder cancer T 24 , 5637 and J82 cells which treated for 48 hours using γ-secretase inhibitor by inverted microscope .The mRNA and protein lev-els of the EMT molecular markers , including E-cadherin , N-cadherin , vimentin and Alpha-smooth muscle actin were examined by RT-PCR and Western blot in bladder cancer cells;Detected the changes of drug resistance and invasion respectively by MTT and Transwell in bladder cancer cells .Results After completely blocking the Notch signaling pathway , the inverted microscope showed that bladder cancer cells became smaller and more disperse ;RT-PCR and Western blot showed the mRNA and protein levels of E-cadherin were up-regulated ( P<0.05 ) , contrast , N-cadherin , vimentin and Alpha-smooth muscle actin were down-regulated ( P<0.05 ); The prolifera-tion of bladder cancer cells were significantly inhibited by MTT test;The number of through microporous membrane cells significantly decreased ( P<0.05 ) shown by Transwell test .Conclusions The Notch signaling pathway is completely blocked that nhibites proliferation and EMT of bladder cancer cells , reduces drug resistance and inva-sion in bladder cancer cells .It suggests that drug resistance and invasiveness of bladder cancer can be changed through EMT which is regulated through notch signaling pathway .

Background:Gastroesophageal reflux disease(GERD)is a common disorder of digestive system,however,some of patients are not responding to conventional 4-8 weeks proton pump inhibitor therapy. Aims:To investigate the effect of digital music gastric electrical pacing on clinical symptoms,anxiety and depression and esophageal motility in patients with refractory GERD. Methods:Fifty-three patients with refractory GERD from Jan. 2014 to Oct. 2015 at the Third Affiliated Hospital,the Third Military Medical University were recruited. All of them fulfilled the Montreal definition of GERD. Digital music gastric electrical pacing was performed for 15 days and the efficacy was evaluated by clinical symptom scoring,Hamilton anxiety scale( HAMA),Hamilton depression scale( HAMD)and esophageal motility manometry. Results:After 15-day treatment,the main symptoms,including heartburn,daytime and nocturnal acid reflux,upper abdominal pain,nausea,and sleep disorder were significantly improved(P < 0. 001). A great proportion of patients were complicated with anxiety and/ or depression at recruitment,and after treatment the scores of HAMA and HAMD were decreased significantly(P < 0. 001). Meanwhile,the lower esophageal sphincter resting pressure(LESP),distal wave amplitude,peristaltic wave duration,speed of peristaltic wave and distal contraction integral after treatment were significantly increased as compared with the baseline values(P < 0. 05). Conclusions:Digital music gastric electrical pacing is effective for treatment of refractory GERD with increase of LESP and esophageal body motility. The clinical symptoms and anxiety and depression are improved simultaneously. Digital music gastric electrical pacing is expected to be a new choice of non-medicine treatment for esophageal motility disorders.

ObjectiveTo study the using of cerrobase as the compensation material in the intensitymodulated radiation therapy (IMRT) implementation and impact factors.MethodsWith therapy planning system (TPS) exported the radiation field intensity file (Dicom RT),through measuring the attenuation coefficient of cerrobase,to calculate the processing depth of AUTIMO 3D CNC corresponding for Dicom RT files at each pixel,then using the processed foam casting of Cerrobase,produced the required IMRT compensator.Through the MATRIXX testing the IMRT compensator in clinical implementation.At the same time we compared the MU of using multi-leaf collimator (MLC) and Cerrobase IMRT compensator for 10patients.ResultsWith cerrobase compensation IMRT can get similar dose or dose distribution to dose produced by TPS for point or plane dose,error is within 5％.To comparison with MLC,using cerrobase compensator has fewer treatment times ( (4.44±0.39) min:(5.71±0.57) min (t =10.82,P =0.000) )and fewer MU (462.5 ± 65.8) MU:(524.5±99.6) MU(t=3.14,P=0.012) ).Conclusions Comparison with MLC IMRT,the cerrobase compensation technique has an important application value with its unique advantages.This research provides an implemented method of IMRT radiotherapy for the primaryhospital.