Objective To study the dynamic changes of DNA content of the fibroblasts in narrow pedicle subdermal vascular network flap (SVNP) during their surviving. Methods Eighteen pieces of surviving skin in pig back with size of 16 cm?8 cm and pedicle wideness of 4 cm were stained with acridine orange, and their DNA contents were measured with the adherent cell analysis and sorting cytometer 570. The tissue samples were taken at 3, 4 ,5 ,and 7 days after operation from different parts of the flap.Results The DNA content of fibroblast in the proximal part of the flap was slightly changed, those in the middle portion increased from 3rd to 5th day and became normal at 7th day, and in the distal portion progressively increased after operation. The results suggested that the transitional part of the flap and skin graft in the middle portion was most active in function and metabolism, and well survived, but the distal portion of the graft was poor in surviving and had a long repairing period. These indicated that local tissue hypoxia stimulated proliferation of fibroblasts and synthesis of the DNA in a short time, but as time wert by, the proliferative speed and DNA synthesis in the individual cell were heterogeneous, probably because of the degeneration or necrosis of some epidermal cells in the graft. Conclusion The wideness and length of the pedicle as well as its vascular anastomoses should be readjusted to promote the blood circulation of the distal skin graft tissues.

The present study was performed to determine whether or not that cardiacrenin-angiotensin system (RAS) plays an important role in volume-overload myocardialhypertrophy. In order to control the increase of circulating RAS, aortacava fistula withnephrectomy (ACF+NT) rats were used in the experiment. The ventricular angiotensin Ⅰ,Ⅱ (Ang Ⅰ,Ⅱ) contents and angiotensin-converting enzyme (ACE) activities were measuredby means of radioimmunoassay and biochemical method. It was shown that in ACF+NTrats, the Ang Ⅱ contents and ACE activities in both left and right ventricles increasedsignificantly despite the plasma Ang Ⅰ, Ⅱ and renin activity remained at a lower level.These results indicate that: (1) Cardiac RAS as a relatively independent system, plays aregulatory role in the development of volume-overload cardiac hypertrophy; (2) The in-crease of Ang Ⅱ content was partly caused by the enhancement of ACE activities; (3) Atleast at the early stage of volume-overload cardiac hypertrophy, the circulating RAS maynot be a necessary promoter.

Objective To investigate the myocardial remodeling inhibition effect of bisoprolol combined with trimetazidine in the treatment of hypertensive myocardial hypertrophy.Methods 86 cases with hypertension and cardiac hypertrophy in Lingwu People’s Hospital of Ningxia from June 2015 to June 2016 were selected and randomly divided into two groups,43 cases in the control group received routine clinical treatment,43 cases in the experiment group received more with bisoprolol combined with trimetazidine treatment.Blood pressure,ultrasonic cardiogram,levels of serum TNF-α, MMP3 and CRP were compared before and after the treatment.Results Compared with before treatment,levels of blood pressure,IVST,LVPWT,LVMI, MMP3,TNF-αand serum CRP significantly decreased after treatment,and compared with the control group,levels of blood pressure,IVST,LVPWT, LVMI,MMP3,TNF-αand serum CRP in the experiment group were lower,the differences were statistically significant (P<0.05),and the effective rate in the experiment group was higher than the control group ( P<0.05 ) .Conclusion Bisoprolol combined with trimetazidine can effectively inhibit the remodeling of hypertension in patients with myocardial hypertrophy,has good clinical efficacy.

Thyroid cancer is one of common malignant tumor in neck, which can occur in any age. Females are more likely to have this disease than males. Most patients get a nodule on their thyroid, which typically does not cause any symptoms. Surgical resection is an effective method to cure thyroid cancer. In recent years, more and more evidence support that traditional Chinese medicine plays an important role in the recovery of postoperative thyroid carcinoma.

Objective To observe the postoperative analgesia effect of ultrasound-guided trans-versus abdominis plane block (TAP)in patients received lower abdominal surgery.Methods Ninety patients undergoing lower abdominal surgery were randomly divided into group A (received TAP), group B (received patient controlled intravenous analgesia with sulfentanil),and group C (received patient controlled epidural analgesia with sulfentanil and ropivacaine)for postoperative analgeda.All patients received combined spinal and epidural anesthesia.VAS score at postoperative 2,4,8,16 and 24 h,as well as the side effects related to postoperative analgesia,were observed and recorded for each patient.Results Comparing with group A,VAS scores of group B at postoperative 2,4,8,16 and 24 h were obviously higher (P< 0.05);Comparing with group A,VAS score of group C at post-operative 24 h was obviously lower (P< 0.05).Comparing with group A,nausea and vomiting,pru-ritus,and somnolence were more obvious in group B (P < 0.05);nausea and vomiting,sensory and motor abnormality of lower limbs and urinary retention occurred more frequently in group C (P <0.05).Conclusion Ultrasound-guided TAP can effectively relieve postoperative pain 24 hours,and has no obvious side effect in patients received lower abdominal surgery.

[Objective]Through the anatomical observation and measurement on middle and distal humerus and radial nerve,To investigete a new radial nerve transfer operation.[Method]By means of anatomical methods,radial nerve's stretching branch,the relationship between radial nerve and deep brachial vessel in 30 upper climbs were observed and measured on 15 adults fixed in 10% formalin.The anatomical structures of humerus of one hundred specimens were also observed.This data were made by designing and performing the radial transfer operation after statistical analyzing.[Result](1)Length of humerus: The left side was (30.60?1.46)cm and the right side(31.37?1.23)cm.The length of sulcus for radial nerve: the left side was(5.65?1.01)cm and the right side(6.57?0.58)cm.The stretch angle of radial nerve: the left was 13.00??1.08?,the right13.86??0.97?.(2)The stretch angle of radial nerve: the radial nerve in the sulcus for radial nerve went from superomidial region to lateral lower region and the angle open to the inner part was formed.The angle is obvious in 29 sides,that was 96.67%,and not obvious only in 1 side,that was 3.33%.(3)The position of the radial nerve superficial and deep branches: The radial nerve in 17 sides branches were above interepicondylar line,which was 76.67%.Below the line were 10 sides,cover 33.33% and on the line 3 sides,cover 10.00%.(4)The curve distance between the exit of radial nerve and the brarnches was(14.26?1.01)cm.(5)The relationship between radial nerve and deep brachial vessel: the radial nerve lies inside of the deep brachial vessel were 12 sides,lies the outside of the deep brachial vessel were 10 sides and cross the deep brachial vessel were 8 sides.[Conclusion]The design of radial nerve transfer operation is reasonable,it conforms to the radial nerve's anatomy and physiology and provides much wider operation space for the performing of all kinds of internal fixation on fractures of middle and distal humerus.It reduces the injury of the radial nerve in the course of the operation and alter the operation while taking the internal fixation substance out.It doesn't increase the difficulty and injury.

To introduce the implication,research overview and appraisal method of patient reported outcome being used in the clinical research of stroke.The basic process and principle of stroke-associated scale research was described in this article,the author think that PRO can be the important supplement of clinical therapeutic e ect evaluation of stroke.The study method of PRO is worth to extension and application in clinical research of other chronic disease.

BACKGROUND:Deproteinized cancellous bone can be used as a new kind of bone graft material due to its wide resources andbiological characteristics if the problem of immunogen can be solved. OBJECTIVE:To study the properties of deproteinized cancellous bone following preparation with physico-chemical methods,in addition,to explore the feasibility of xenogeneic deproteinized cancellous bone used as scaffolds in bone tissue engineering. DESIGN,TIME AND SETTING:A comparison observation was performed at the Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy between February 2008 and January 2009. MATERIALS:Deproteinized bone was derived from pigs and goals femoral cancellous bone followed by treating with 1:1 methanol extract and 30% H2O2. METHODS:The mechanical properties of vector were examined by scanning electron-microscopy,X-rays diffraction analysis,differential scanning calorimetry,and mechanical experimental instrument. The density of third passage of human bone mesenchymal stem cells (MSCs) of iliac bone was regulated to 2?108/L,vaccinated to vector,and the cell adhesion and proliferation was observed by inverted microscope and scanning electron microscope. Forty BALB/C mice were randomly dividedinto the experimental and control groups,with 20 animals in each group. Muscle behind femur was made muscle pouch,which was implanted pig deproteinized bone in the experimental group,and no other treatment in the control group. The level of serumcarnine,glutamic-oxal(o)acetic transaminase (GOT),as well as glutamic-pyruvic transaminase (GPT) were measured at weeks 12,4,8 after operation. Meanwhile,the immunogenicity of pig deproteinized bone and specimen of deproteinized bone in mice were detected at the 4 weeks after implantation. MAIN OUTCOME MEASURES:Physicochemical properties and immunogenicity of deproteinized bone were analyzed by in vitroexperiment. And the bio-safety was evaluated by animal experiment. RESULTS:The inorganic composition of deproteinized cancellous bone was hydroxyapatite ceramic,which is identical with nature composition of human bone. It showed that deproteinized cancellous bone maintained natural network pore system and kept good mechanical property. The MSCs grew well on deproteinized cancellous bone due to its low immunogenicity and toxicity. CONCLUSION:The xenogeneic deproteinized cancellous bone is an excellent vector in bone tissue engineering.

Objective To compare the demographic and anthropometric characteristics of IFH,IPH and CH in a Chinese newly diagnosed diabetes population and to evaluate the metabolic profiles of insulin secretion and insulin sensitivity associated with subcategories of newly diagnosed type 2 diabetes.Methods From Mar.2005 to June 2002,in Qingdao 335 patients with newly diagnosed diabetes were classified into three groups:IFH(n=67),IPH(n=92),and CH(n=176).Meanwhile,411(191males)subjects with age-sex matched were selected as control group. Homeostasis model assessment(HOMA-IR)and LIGUANGWEI index(IAI)were applied to assess the status of insulin resistance.Homeostasis model assessment(HOMA-B)and I_0/G_0 were applied to assess the basic function of islet B cell,and I_ 120 /G_ 120 was applied to assess the postload function of islet B cell. Results HOMA-IR increased and IAI decreased significantly in CH group than in IFH and IPH group(P

Objective To study the tactics, methods and relevant factors of transcatheter embolization for acute gastrointestinal hemorrhage.Methods Fifteen patients with acute gastrointestinal hemorrhage were embolized by one of the methods of Polyvinyl Alcohol (PVA), gelfoam or metal coils. Four of the fifteen patients were upper gastrointestinal hemorrhage, the other cases were lower gastrointestinal hemorrhage which were embolized using coaxial microcatheter. Results Fourteen of the fifteen patients were treated successfully by these methods. There were total 17 times of embolization for 16 parts, the success rate reached 94.1%. The other one reveaded an infarction of intestine after the embolization and was cured by resection. One of the fifteen patients appeared a recurrent hemorrhage 3 months later, and confirmed to be a hemangiolymphangioma. Two patients with malignant tumor were operated upon selectively. The other patient of intestinal hemorrhage was embolized successfully by using a metal coil after shock. Leiomyoma complicated with large area of bleeding was finally proven by operation.Conclusions Transcatheter embolization for acute massive gastrointestinal hemorrhage is safe and efficient under different choice of methods. The key of success is the right selection of embolized target artery and dosage of emboli.