Adult ; Aortic Stenosis, Supravalvular ; Child ; Female ; Humans ; Mothers

ObjectiveTo investigate the complication and nursing of intestines nutrition in critical patients after operation. MethodsThe clinical states of 40 patients after operation and being brought to ICU were analyzed.ResultsPatients accepting intestines nutrition presented high incidence of gastrointestinal complications. ConclusionIt is important to pay attention to prevent gastrointestinal complications in patients accepting intestines nutrition early postoperation.

Adult ; Ear Canal ; Ear Neoplasms ; Humans ; Male ; Sarcoma, Myeloid

Adolescent ; Altitude ; Anaerobic Threshold ; physiology ; Drugs, Chinese Herbal ; therapeutic use ; Emigrants and Immigrants ; Humans ; Hypoxia ; physiopathology ; prevention & control ; Male ; Oxygen Consumption ; physiology ; Physical Endurance ; physiology ; Phytotherapy ; Young Adult

Objective To observe the effeet of cotransduction of wt-p53,B7-1 and GM-CSF genes mediated by recombinant adenovirus on cell growth, apoptosis and immunogenicity of neuroblastoma cell line SH-SY5Y, Methods We transfected SH-SYSY cells separately with recombinant adenovirus, recombinant adenovirus mediating human wild type p53(wt-p53) gene and BB-102.We separately named them with SH/Ad,SH/p53 and SH/BB-102 group.The protein expression of p53, B7-1 and GM-CSF were measured by Western blot, FCM and enzyme linked immunosorbent assay. Cell number was counted and growth curves were drawed. Cell apoptosis was tested by FCM. Lymphocyte proliferation and cytokine secretory was evaluated by mixed lymphocyte culturing. Results After transection with BB- 102, p53, B7-1, and GM-CSF, high-efficiency expression of target genes were found. Growth of the cells was inhibited, apoptosis was induced in the SH/p53 and SH/BB-102 groups. Proliferation of lymphocytes was stimulated and cytokine increased visorously in the SH/BB-102 group. Condusions After transfection with BB-102, growth of the SH-SY5Y cells was inhibited,apoptosis was induced,and immunogenicity was greatly enhanced.

The nafion/carbon nanoparticles (CNPs) composite film modified glassy carbon electrode was pre pared by dropwise method, and the electrocatalyst of H_2O_2 at Nafion/CNPs modified electrode was investiga ted.The results show that electrocataytic activitie for detection of H_2O_2 at the modified electrode is very good.The biosensing application of CNPs was demonstrated through fabrication of an electrochemical biosensor.The biosensor was constructed by encapsulating glucose oxidase in the Nafion/CNPs composite film.The biosensor had good electrocatalytic activity toward oxidation of glucose.The glucose biosensor shows a linear range from 2.0 × 10 ~(-6) to 6.0×10~(-3) mol/L with a detection limit of 1.6×10 ~(-6) mol/L.The biosensor shows high stabili ty, good reproducibility and can avoid the commonly coexisted interference.In addition, real rat serum samples were analyzed by this biosensor with satisfactory results.

【Objective】In order to establish the foundation for transgenic mouse model,the human thalassemic gene(β654) was cloned and sequenced.【Methods】The human β654 gene was amplified by PCR,and cloned into the plasmid BGT51 in which the human β gene was cut out aforehand.The recombinant plasmid was certified by enzyme-digestion,reverse dot hybridization and sequencing.【Results】A recombinant plasmid was obtained,which contained the human β654 gene in the correct recombinant direction.Sequencing showed that the cloned insert was correct.【Conclusions】The recombinant plasmid constructed is useful for establishing a transgenic mouse.

Objective To evaluate the feasibility of laparoscopic hepatectomy.Methods A total of 27 patients received laparoscopic hepatectomy by using laparoscopic Peng's multifunctional operative dissector(LPMOD).Among the patients,13 underwent anatomic hepatectomy(resection of the Ⅱ and Ⅲ segments in 3,Ⅴ segment in 1,Ⅵ in 7,Ⅴ and Ⅵ in 1,and Ⅴ,Ⅵ,Ⅶ,and Ⅷ segments in 1),and 14 received partial hepatectomy.Results The laparoscopic hepatectomy was accomplished successfully in all the patients without converting to open surgery.The mean operation time was 35-360 min [mean,(178.0?78.4) min];intraoperative blood loss was 35-1200 ml [mean,(451.7?332.6) ml];and the postoperative hospital stay was 2-14 d [mean,(8.4?3.0) d].No severe complication occurred after the operation.The postoperative pathological examinations showed hepatic hemangioma in 15,focal hyperplasia of the liver in 2,hepatocelluar carcinoma complicated with liver cirrhosis in 6,intrahepatic bile duct stone complicated with hyperplasia of the bile duct in 2,liver(hematoma) fibrosis in 1,and hepatic angiomyolipoma in 1.The patients were followed up for 1-26 months [mean,(7.9?7.4) months] by using B-ultrasonography or CT.No recurrence of hemangioma or carcinoma,tumor implantation at the puncture site,or residual stones in the intrahepatic bile duct was found in this series.Conclusion By using LPMOD,laparoscopic hepatectomy is safe and feasible for selected patients.

Objective: To investigate the association between the single nucleotide polymorphism (SNP) of mitogen-activated protein kinase kinase 4 (MKK 4) promoter and the risk of lung cancer development in Southern Chinese population. Methods: A hospital-based case-control study including 800 cases of lung cancer and 900 healthy controls was conducted. TaqMan assay was used to test the SNP of rs3826392 (-1304T>G) in MKK 4 promoter, and the software SAS 9.13 was used to analyze the association of MKK4 polymorphism and the susceptibility of lung cancer. Results: The observed genotype frequency of -1304T>G in MKK4 promoter was appropriate for Hardy-Weinberg equilibrium in the healthy controls (P = 0.149). The distribution of genotypes was significantly different between the cases of lung cancer and the healthy controls (P=0.001). Compared with homozygous genotype (TT), the risk for lung cancer was decreased by 25% in the carriers of heterozygous genotype (TG) [adjusted odd ratio (OR ) = 0.75, 95% confidence interval (CI) = 0.58-0.97)], and the risk for lung cancer in carriers of GG homozygote was decreased by 45% (adjusted OR = 0.55, 95% CI = 0.33-0.94). There was a significantly decreased trend in the risk for lung cancer along with the increased number of mutation-typic G allele (Ptrend<0. 001). Conclusion: These findings demonstrate that -1304T>G genotype in promoter region of MKK4 gene may contribute to the decreased risk of lung cancer. Copyright© 2011 by TUMOR.

Objective To establish the relationship of serum TNF-? and IL-6 contents with myocardial cell apoptosis in the chronic heart failure (CHF)rats,and to explore the therapeutic mechanism of Anxin Granules (AXG)in treating CHF.Methods Fifty female SD rats were randomly divided into five groups:normal control group (A),model group (B),captopril group(C),low-dosage AXG group (D)and high-dosage AXG group (E).CHF models were established by intraperitoneal injection of small-dose of adriamycin,once per week for continuous 6 weeks.Meanwhile,group C was given captopril injection (6.25mgkg-1d-1),and groups D and E received gastric gavage of low-dosage AXG (0.8 gkg-1d-1)and high-dosage AXG (1.6gkg-1d-1)respectively.After 6 weeks of modeling,serum levels of TNF-? and IL-6 of different groups were tested by ELISA,the apoptosis of myocardial cells was tested by TUNEL,and then the apoptosis index(AI)was counted.Results Serum levels of TNF-? and IL-6 were increased in the model group (P