Based on ESI database, the paper carries out bibliometric analysis on international psychiatry and psychology literatures during 2004-2014, through comparison of country/region, institutions and journal distribution, it demonstrates the academic status and weakness of our country in psychiatry and psychology field, providing references psychiatry and psychology scientific research development in our country.

Objective To observe the effect of Xuesaitong injection on the platelet activity in the patient underwent hip orthopedics operation. Methods A total of 80 patients underwent hip orthopedics operation were enrolled from our Hospital. The patients were divided into the control group (n=40) and the observation group (n=40). The control group was treated with the low molecular weight heparin at 12 h before operation and after operation. The observation group was treated with Xuesaitong injection at 12 h before operation and after operation. The deep venous thrombosis (DVT) was diagnosed by color doppler ultrasound. The APTT and PT weredetected by the automated blood coagulation analyzer. The expression of TXB2 was determined by ELISA analysis. The CD62P was detected by the flow cytometry. Results At time of 10 days after operation, the APTT (26.83 ± 3.68 s vs. 32.06 ± 3.65 s, t=2.407), PT (10.84 ± 1.63 s vs. 12.82 ± 1.61 s, t=2.391), TXB2 (159.38 ± 31.75 ng/L vs. 194.23 ± 38.53 ng/L, t=2.388), CD62P (14.28%± 2.52%vs. 29.14%± 6.23%, t=6.415) were significantly lower in the observation group than those in the control group (P<0.01 or P<0.05). The incidence of DVT was 12.5%in the observation group, and 17.5%in the control group. There was no significant difference between the two groups (χ2=0.353, P=0.241). Conclusions The Xuesaitong injection can inhibit platelet activation and has preventive effect of DVT in patient underwent hip orthopedics operation with no bleeding complications.

Objective To review the CT appearances and important differential diagnoses of various primary and secondary mesenteric neoplasms.Methods By describing the mesenteric anatiomy and major routes for the dissemination of metastatic mesenteric tumors, the article presents both the common and rare types of various primary and secondary mesenteric neoplasms, and addresses the characteristic CT appearances and important aspects of the differential diagnosis.Results CT study, especially the multi-slice spiral CT (MSCT), along with the clinical history and other related information, can nicely depict various mesenteric tumors and well differentiate them from infectious, inflammatory or vascular processes affecting the mesentery.Conclusion CT is the imaging method of choice for the evaluation of tumors of small bowel mesentery.

Animals ; Cadherins ; metabolism ; Carcinoma, Hepatocellular ; metabolism ; pathology ; Catenins ; metabolism ; Epithelial-Mesenchymal Transition ; drug effects ; genetics ; Humans ; Liver Neoplasms ; metabolism ; pathology ; MicroRNAs ; genetics ; metabolism ; Transforming Growth Factor beta ; pharmacology

Objective　This study was to establish enzyme-rate method for determining serum beta-hydroxybutyric acid ,and to discuss its meaning in diabetes ketosis acidism. Methods　Enzyme-rate method was used to determine serum beta-hydroxybutyric acid in 60 cases of normals, 30 cases of diabetes ,and 8 cases of ketosis acidism by Hitachi 7170A autochemistry analyzer. Results　The recovery rate is 108%,the linerity extent 0-4.0 mmol.L-1, the coefficient of variation within-run is 2.2%and day-to-day is 2.6%. Conclusion　The enzyme-rate method for determining serum beta-hydroxybutyric acid save time, gives accurate results and has wide linerity,so it can used for diagnosis of diabetes ketosis acidism.

Primer pair was designed as self-template during the amplification by PCR. It’s an easy way to construct a series of multi-copy genes. This method can be available for the amplification of DNA probes and the development of vaccines. It’s considered as a convenient and simple method for the cloning of multi-copy genes.

Purpose　To study the effect of heparin on expression of type collagen Ⅰ,ⅢmRNA in cultured fetal rat lung fibroblast (LFb). Methods　By using Northern blot method and immage analysis,the expression of collagenⅠ, Ⅲ mRNA were quantitatively analysed in the fibroblasts treated with different concentration heparin (0.1 mg/L, 1 mg/L, 10 mg/L, 100 mg/L)separately. Results　Heparin treatment at 0.1 mg/L,1 mg/L, 10 mg/L and did not influence the expression of collagen Ⅰ,Ⅲ mRNA，but the expression of collagen Ⅰ,Ⅲ mRNA were inhibited at 100 mg/L. Conclusion　Expression of collagen Ⅰ, ⅢmRNA is related to the concentration of heparin.

The identification and classification of Thermus to Thermales is revie wed. Up to now, the order Thermales is consist of only one family Thermaceae, w h ich includes 4 genera with 14 valid species about 50 strains, and with 4 unvalid spcecies and more than 50 strains unidentified. The morphology, physiology, G+ C mol%, DND hybridization, fatty acid components, 16S rRNA alignment and secondar y structure analyses have been tools for the taxonomy of Thermales.

To develope a 5 lipoxygenase inducible expression system for further understanding the function and signal transduction pathway of 5 lipoxygenase, providing information for drug screening.The 2 kb 5 LO cDNA sequence containing HindⅢ site was generated by PCR using pEGFP 5LO as a template. Full length 5LO cDNA was subsequently cloned into the corresponding Pst Ⅰ and Sal Ⅰ sites of the plasmid pBI G to generate the mammalian expression vector pBI 5LO. HeLa Tet On cell line was co transfected with pBI 5LO and pTK Hyg plasmids. Inducible expression of target gene was detected using X gal histochemical staining of cell monolayers for ? galactosidase after incubating the transfected cell line with 1 ?g/ml doxycycline for 48 h.The HindⅢ restriction digest site was inserted into polyclonal site of pBI G and recombinant vector pBI 5LO was constructed. The desired recombinant plasmid pBI 5LO was identified by restriction analysis, and orientation and junctions were confirmed by sequencing. Induction of the target gene expression by doxcycline treatment was demonstrated by X gal histochemical staining and nuclei of the cells that have expressed the pBI 5LO expression vector were brilliant blue.The 5LO inducible expression system is established successfully and it works well in cell model.

[Objective] Probing into the effect of Changyanning syrup about colonic electrophysiology and motility of visceral hypersensitivity rat.[Methods] The test consists of the model series and the control series and the Changyanning syrup series,and the two latter rats were sensitized by intraperitoneal injection of chicken egg albumen.After two weeks,the stomach is poured 0.7ml's normal saline by blank space series,0.7ml's normal saline by model series,0.7ml's Changyanning syrup by Changyanning series.Continue to pour stomach in a month,writing colon fast wave,slow wave and the colon contraction,the observation of interdigestive myoelectric complex IMC cycle,Ⅲ times duration,fast wave and slow wave propagation rate,the largesttest amplitude,the amount of the colon contraction,the index of the colon contraction.[Result] The model series' interdigestive myoelectric complex IMC cycle prolongs,Ⅲ times duration prolongs,fast wave and slow wave propagation rate speeds up,the largesttest amplitude broadens.The slow wave propagation rate speeds up.The amount of the colon contraction speeds up,the index of the colon contraction speeds up.After Changyanning series treated,interdigestive myoelectric complex IMC cycle cuts down,Ⅲ times the duration cuts down,the largesttest amplitude decreases.[Conclusion] There are quite differencs between model group and control group in electricity and motion of visceral hypersensitivity rat.Changyanning syrup treating visceral hypersensitivity is by means of partly recovering the colon fast and slow wave and contraction wave rhythm and amplitude.