1.Detection of Helicobacter DNA in bile from bile duct diseases.
Im Hwan ROE ; Jung Taik KIM ; Hak Sung LEE ; Jong Hwa LEE
Journal of Korean Medical Science 1999;14(2):182-186
Several species of Helicobacter colonize the hepatobiliary tract of animals and cause hepatobiliary diseases. The aim of this study is to investigate Helicobacter found in the biliary tract diseases of humans. Thirty-two bile samples (15 from bile duct cancer, 6 from pancreatic head cancer, and 11 from intrahepatic duct stone) were obtained by percutaneous transhepatic biliary drainage. Polymerase chain reaction analysis using Helicobacter specific urease A gene and 16S rRNA primers, bile pH measurement, and Helicobacter culture were performed. Helicobacter DNA was detected in 37.5%, and 31.3% by PCR with ureA gene, and 16S rRNA, respectively. The bile pH was not related to the presence of Helicobacter. The cultures were not successful. In conclusion, Helicobacter can be detected in the bile of patients with bile duct diseases. The possibility of pathogenesis of biliary tract diseases in humans by these organisms will be further investigated.
Adenocarcinoma/microbiology
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Adult
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Aged
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Aged, 80 and over
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Bile/microbiology*
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Bile Duct Diseases/microbiology*
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Bile Duct Neoplasms/microbiology
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Cholelithiasis/microbiology
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DNA Primers
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DNA, Bacterial*
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Helicobacter/isolation & purification*
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Helicobacter/growth & development
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Helicobacter/genetics
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Human
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Hydrogen-Ion Concentration
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Middle Age
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Pancreatic Neoplasms/microbiology
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Polymerase Chain Reaction
2.Clinical Significance of White Bile (Bilirubin-Free Bile) in Malignant Bile Duct Obstruction.
Jin Tae JUNG ; Ho Gak KIM ; Jimin HAN ; Joong Goo KWON ; Chang Hyeong LEE ; Eun Young KIM
The Korean Journal of Gastroenterology 2008;52(2):91-96
BACKGROUND/AIMS: White bile is colorless, translucent fluid found occasionally in malignant bile duct obstruction (MBO). Little information is available on the cause and effect of white bile. The aim of this study was to determine the frequency and clinical significance of white bile in MBO. METHODS: Bile was aspirated during endoscopic retrograde cholangiopancreatography in consecutive patients with MBO. White bile was defined as bile bilirubin <1.5 mg/dL and yellow bile was defined as bile bilirubin >or=1.5 mg/dL in the bile. Two groups were compared prospectively for the duration of jaundice, itching, cholangitis, level of obstruction, and decremental rate of bilirubin after the insertion of 7 Fr endoscopic nasobiliary drainage until the insertion of metal stent or 10 Fr plastic stent. RESULTS: Among 60 patients with MBO, 16 (26.7%) had white bile. WBC count in blood was higher (9,456/mm3 vs. 7,400/mm3, p=0.029) and cholangitis was more common (11/16 vs. 7/44, p=0.000) in white than yellow bile group. Proximal portion of MBO had no communication with GB in 9/16 patients with white bile group and 17/44 patients with yellow bile group (p>0.05). Mean survival of the inoperable 35 patients was 242 days in yellow bile and 227 days in white bile group (p>0.05). CONCLUSIONS: White bile in MBO was not rare and was associated with cholangitis. Gallbladder did not seem to play a role in the formation of white bile. Further study for the pathogenesis and prognosis of white bile in MBO will be necessary.
Aged
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Bile/*chemistry/microbiology
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Bile Duct Neoplasms/*diagnosis/etiology/mortality
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Bilirubin/analysis
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Cholangiopancreatography, Endoscopic Retrograde
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Cholangitis/diagnosis/etiology/mortality
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Cholestasis/*diagnosis/etiology/mortality
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Data Interpretation, Statistical
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Drainage
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Female
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Humans
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Male
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Middle Aged
;
Stents
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Survival Analysis
3.A case of emphysematous hepatitis with spontaneous pneumoperitoneum in a patient with hilar cholangiocarcinoma.
Jung Ho KIM ; Eul Sik JUNG ; Seok Hoo JEONG ; Ju Seung KIM ; Yang Suh KU ; Ki Baik HAHM ; Ju Hyun KIM ; Yeon Suk KIM
The Korean Journal of Hepatology 2012;18(1):94-97
An 80-year-old woman with hilar cholangiocarcinoma was hospitalized due to sudden-onset abdominal pain. Computed tomography revealed hepatic necrosis accompanied with emphysematous change in the superior segment of the right liver (S7/S8), implying spontaneous rupture, based on the presence of perihepatic free air. Although urgent percutaneous drainage was performed, neither pus nor fluids were drained. These findings suggest emphysematous hepatitis with a hepatic mass. Despite the application of intensive care, the patient's condition deteriorated rapidly, and she died 3 days after admission to hospital. Liver gas has been reported in some clinical diseases (e.g., liver abscess) to be caused by gas-forming organisms; however, emphysematous hepatitis simulating emphysematous pyelonephritis is very rare. The case reported here was of fatal emphysematous hepatitis in a patient with hilar cholangiocarcinoma.
Aged, 80 and over
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Anti-Bacterial Agents/therapeutic use
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Bile Duct Neoplasms/complications/diagnosis
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Bile Ducts, Intrahepatic/pathology
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Cefotaxime/therapeutic use
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*Cholangiocarcinoma/complications/diagnosis
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Clostridium Infections/drug therapy/microbiology
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Clostridium perfringens/isolation & purification
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Emphysema/complications/*diagnosis
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Escherichia coli/isolation & purification
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Escherichia coli Infections/drug therapy/microbiology
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Female
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Hepatitis/complications/*diagnosis
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Humans
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Metronidazole/therapeutic use
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*Pneumoperitoneum/complications/diagnosis
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Tomography, X-Ray Computed