Objective To study the processing method,extraction technology and quality evaluation analysis of Paeonialactiflora Pall, so as to provide reference for selecting the best method and improving its quality .Methods One year and 3 years Chinese herbal medicine Paeonialactiflora Pall was used.The use of raw materials and cooked products for processing methods to paeoniflorinas the representative , and the content was determined by high performance liquid chromatography.With the optimization of orthogonal test, the methods for the determination of effective components of radix paeoniaealba were summarized and the quality control methods were analyzed .Results The content of paeoniflorin in raw paeonialactiflora pall was (6.33 ±0.52)%,and the content of paeoniflorinin cooked Paeonialactiflora Pall was (5.74 ±0.45)%, which was with statistical significance(P <0.05).The content of paeoniflorin in raw paeonialactiflora pall was (5.90 ±0.38)%,and 4.85 ±0.45% in three years cooked paeonialactiflora pall, and the differenceswere with statistical significance(P<0.05).Extracted three times with 70% ethanol, time 65 min, 6 times the amount of solvent,the paeoniflorin high cream rate was highest.By means of induction, several active ingredients should be screened out to control the content of paeonialactiflora pall to publish multiple indicatorsquality control methods .Conclusion The preparation method of Chinese herb paeonialactiflora pall has showed that the active ingredient content is morein raw herb.To establish quality control method of multiple indicators of paeonialactiflora pall based on raw herb,gradually improve the quality of more comprehensive paeonialactiflora pall quality control model.To optimize the extraction of Chinese herbal medicine,provide scientific reference for the sustainable utilization and industrial production of paeonialactiflorapall.

Objective To understand the clinical characteristics during clinical diagnose and treatment of young women patients under 35 years with cervical cancer. Methods The clinical data of 95 cervical cancer patients under 35 years was reviewed and analysed retrospectively. Results The percentage of the patients at this age set was 18. 59% ,accounting for almost one fifth of all the same period patients with cervical cancer. The most common primary clinical representation was sexual bleeding of vigina (90.53% ,86/95) ;28 cases (29. 47%, 28/95) were misdiagnosed by naked eye examination and local treated as cervicitis; 13 cases (13. 68%, 13/95) with severe pathological changes were missed diagnosed by biopsy under colposcopy ;53 cases (55. 79% ,53/95) had their ovary preserved by one or two and had no metastasis or recurrence with carcinoma. Conclusions Sexual intercourse bleeding is the most common manifestation of young women with cervical cancer,which demands more attention. The biopsy under colopscopy has certain limitations,and should be performed along with conization while necessary. It is safe and feasible to retain ovary in cervical cancer patients before Ⅱ stage.

Objective To study on algicidal bioactive substances from mangrove bacteria and its algicidal effect on red tide algae Alexandriam tamarense.Methods A red algicidal bioactive substance in Guangxi mangrove bacteria was isolated and purified by Silica-gel Column Chromatography,and its structure was determined on the basis of UV,IR,1H NMR,13C NMR and HREIMS spectroscopic analyses and comparison with the literatures.Its algicidal affect was primarily studied in this paper.Results and Conelusion The results showed that the red algicidal bioactive substance was prodigiosin,which had an inhibitory effect on the cells growth of Alexandrium tamarense,and the inhibitory effect increased with the increase of prodigiosin concentration.The prodigiosin stimulated the production of MDA and reduced the content of sulfhydryl group.In addition,plasma membrane permeability of leaked cell solution was increased.These indicated the extinguishing mechanism: the cell membrane was damaged followed by the inhibition of the cell growth.

Objective To investigate the effects of dapagliflozin on inflammatory cytokines,blood glucose control and cardiac function in elderly with preserved ejection fraction heart failure(HFpEF)combined with type 2 diabetes.Methods A total of 80 elderly HFpEF patients with type 2 diabetes mellitus were randomly divided into control group(40 cases)and study group(40 cases).The control group received hypoglycemic and anti-heart failure standard therapy,and the study group received hypoglycemic and anti-heart failure standard therapy and dapagliflozin therapy.Both groups were treated for 6 months.The levels of inflammatory factors,blood glucose control,myocardial markers,exercise endurance[6 min walking distance(6MWD)],cardiac ultrasound related indexes and adverse drug reactions were compared between the two groups.Results After 6 months of treat-ment,serum interleukin-6(IL-6),hypersensitive C-reactive protein(hs-CRP)and tumor necrosis fac-tor(TNF)-α in the study group were significantly lower than those in the control group(P＜0.05);the glycated hemoglobin(HbA1c),fasting blood glucose(FPG),amino terminal B-type brain natriuretic peptide precursor(NT-proBNP)and hypersensitive troponin(hs-TNT)of the study group were significantly lower than those of the control group(P＜0.05);the 6MWD and left ven-tricular ejection fraction(LVEF)of the study group were higher than those of the control group,and the left ventricular end-diastolic diameter(LVEDD),right atrial and right ventricular diameter,left atrial and left ventricular diameter,right ventricular wall thickness and pulmonary artery pressure of the study group were significantly lower than those of the control group(P＜0.05).There was no significant difference in the incidence of adverse reactions between the two groups(P＞0.05).Conclusion The use of dapagliflozin in the treatment of elderly HFpE patients complicated with type 2diabetes can effectively control their blood sugar level,reduce inflammation,improve exercise endurance,and improve patients'heart function without increasing adverse drug reactions.

Objective To investigate the effects of dapagliflozin on inflammatory cytokines,blood glucose control and cardiac function in elderly with preserved ejection fraction heart failure(HFpEF)combined with type 2 diabetes.Methods A total of 80 elderly HFpEF patients with type 2 diabetes mellitus were randomly divided into control group(40 cases)and study group(40 cases).The control group received hypoglycemic and anti-heart failure standard therapy,and the study group received hypoglycemic and anti-heart failure standard therapy and dapagliflozin therapy.Both groups were treated for 6 months.The levels of inflammatory factors,blood glucose control,myocardial markers,exercise endurance[6 min walking distance(6MWD)],cardiac ultrasound related indexes and adverse drug reactions were compared between the two groups.Results After 6 months of treat-ment,serum interleukin-6(IL-6),hypersensitive C-reactive protein(hs-CRP)and tumor necrosis fac-tor(TNF)-α in the study group were significantly lower than those in the control group(P＜0.05);the glycated hemoglobin(HbA1c),fasting blood glucose(FPG),amino terminal B-type brain natriuretic peptide precursor(NT-proBNP)and hypersensitive troponin(hs-TNT)of the study group were significantly lower than those of the control group(P＜0.05);the 6MWD and left ven-tricular ejection fraction(LVEF)of the study group were higher than those of the control group,and the left ventricular end-diastolic diameter(LVEDD),right atrial and right ventricular diameter,left atrial and left ventricular diameter,right ventricular wall thickness and pulmonary artery pressure of the study group were significantly lower than those of the control group(P＜0.05).There was no significant difference in the incidence of adverse reactions between the two groups(P＞0.05).Conclusion The use of dapagliflozin in the treatment of elderly HFpE patients complicated with type 2diabetes can effectively control their blood sugar level,reduce inflammation,improve exercise endurance,and improve patients'heart function without increasing adverse drug reactions.

Objective To evaluate the clinical efficacy of lymphoplasma exchange (LPE) for the treatment of severe refractory immune-related skin diseases.Methods From May 2013 to October 2015,8 patients with toxic epidermal necrolysis,drug-induced hypersensitivity syndrome (DIHS),pemphigus vulgaris,pemphigoid or paraneoplastic pemphigus were enrolled from Department of Dermatology,Xiangya Hospital,Central South University,who showed no response to conventional therapy or presented with multiple organ dysfunction.After the treatment with LPE,the efficacy was evaluated,and adverse reactions were observed.Results After one session of LPE therapy,6 patients received marked improvement,and were cured at last.In 1 patient with pemphigus vulgaris who was resistant to the treatment with high doses of glucocorticoids and immunosuppressive agents,the rashes regressed during the treatment with LPE,but recurred after the end of treatment.One patient with bullous pemphigoid presented with eruptive blisters on the next day after the treatment with LPE,which were considered as allergic reactions to allogeneic plasma.There were no obvious differences in white blood cell count,lymphocyte count,neutrophil count and blood platelet count in the peripheral blood of 8 patients before and after the treatment with LPE.During the follow-up of 3-5 years,all of the patients were recovered without recurrence,except 1 patient with bullous pemphigoid who died of disseminated tuberculosis after 1 year.Conclusion LPE is effective for the treatment of severe immune-related skin diseases,but attention should be paid to potential transfusion reaction and allergic reactions.

Patients with severe trauma require an extremely timely treatment and transfusion plays an irreplaceable role in the emergency treatment of such patients. An increasing number of evidence-based medicinal evidences and clinical practices suggest that patients with severe traumatic bleeding benefit from early transfusion of low-titer group O whole blood or hemostatic resuscitation with red blood cells, plasma and platelet of a balanced ratio. However, the current domestic mode of blood supply cannot fully meet the requirements of timely and effective blood transfusion for emergency treatment of patients with severe trauma in clinical practice. In order to solve the key problems in blood supply and blood transfusion strategies for emergency treatment of severe trauma, Branch of Clinical Transfusion Medicine of Chinese Medical Association, Group for Trauma Emergency Care and Multiple Injuries of Trauma Branch of Chinese Medical Association, Young Scholar Group of Disaster Medicine Branch of Chinese Medical Association organized domestic experts of blood transfusion medicine and trauma treatment to jointly formulate Chinese expert consensus on blood support mode and blood transfusion strategies for emergency treatment of severe trauma patients ( version 2024). Based on the evidence-based medical evidence and Delphi method of expert consultation and voting, 10 recommendations were put forward from two aspects of blood support mode and transfusion strategies, aiming to provide a reference for transfusion resuscitation in the emergency treatment of severe trauma and further improve the success rate of treatment of patients with severe trauma.