This essay is to present an improvement on the concentration assay for sodium chloride in bicarbonate dialysate.
Bicarbonates ; analysis ; chemistry ; Dialysis Solutions ; analysis ; chemistry ; Sodium Chloride ; analysis

Orthogonal combination design was adopted in examining the Spirulina platensis (S. platensis) yield and the influence of four factors (Se content, Se-adding method, S content and NaHCO3 content) on algae growth. The results showed that Se content, Se-adding method and NaHCO3 content were key factors in cultivation conditions of Se-enriched S. platensis with the optimal combination being Se at 300 mg/L, Se-adding amount equally divided into three times and NaHCO3 at 16.8 g/L. Algae yield had a remarkable correlation with OD560 and floating rate by linear regression analysis. There was a corresponding relationship between effects of the four factors on algae yield and on OD560, floating rate too. In conclusion, OD560 and floating rate could be served as yield-forming factors.
Bicarbonates ; analysis ; Culture Media ; Cyanobacteria ; growth & development ; Selenium ; analysis ; pharmacology

During the routine tests, it's found that the testing method related to Hemodialysis mentioned in the industry standard YY0598-2006 cannot reflect the real bicarbonate concentration in Hemodialysis. To discuss the reaction principle and its interference factors deeply by studying the assay determination of bicarbonate in Hemodialysis, and it could be cited as reference for amending the quality standard or troubleshooting. The improved way of determining bicarbonate concentration in Hemodialysis is also put up.
Bicarbonates ; analysis ; Dialysis Solutions ; analysis ; standards ; Renal Dialysis ; methods ; standards

BACKGROUND: The objective of this study was to examine the correlation between arterial blood gas (ABG) and peripheral venous blood gas (VBG) samples for all commonly used parameters in patients admitted to a medical intensive care unit (ICU). METHODS: A single-center, prospective trial was carried out in a medical ICU in order to determine the level of correlation of ABG and peripheral VBG measurements. A maximum of five paired ABG-VBG samples were obtained per patient to prevent a single patient from dominating the data set. RESULTS: Regression equations were derived to predict arterial values from venous values as follows: arterial pH=-1.108+1.145xvenous pH+0.008xPCO2-0.012xvenous HCO3+0.002xvenous total CO2 (R2=0.655), arterial PCO2=88.6-10.888xvenous pH+0.150xPCO2+0.812xvenous HCO3+0.124xvenous total CO2 (R2=0.609), arterial HCO3=-89.266+12.677xvenous pH+0.042xPCO2+0.675xvenous HCO3+0.185xvenous total CO2 (R2=0.782). The mean ABG minus peripheral VBG differences for pH, PCO2, and bicarbonates were not clinically important for between-person heterogeneity. CONCLUSION: Peripheral venous pH, PCO2, bicarbonates, and total CO2 may be used as alternatives to their arterial equivalents in many clinical contexts encountered in the ICU.
Bicarbonates ; Blood Gas Analysis ; Humans ; Hydrogen-Ion Concentration ; Critical Care ; Intensive Care Units ; Prospective Studies

BACKGROUND: The objective of this study was to examine the correlation between arterial blood gas (ABG) and peripheral venous blood gas (VBG) samples for all commonly used parameters in patients admitted to a medical intensive care unit (ICU). METHODS: A single-center, prospective trial was carried out in a medical ICU in order to determine the level of correlation of ABG and peripheral VBG measurements. A maximum of five paired ABG-VBG samples were obtained per patient to prevent a single patient from dominating the data set. RESULTS: Regression equations were derived to predict arterial values from venous values as follows: arterial pH=-1.108+1.145xvenous pH+0.008xPCO2-0.012xvenous HCO3+0.002xvenous total CO2 (R2=0.655), arterial PCO2=88.6-10.888xvenous pH+0.150xPCO2+0.812xvenous HCO3+0.124xvenous total CO2 (R2=0.609), arterial HCO3=-89.266+12.677xvenous pH+0.042xPCO2+0.675xvenous HCO3+0.185xvenous total CO2 (R2=0.782). The mean ABG minus peripheral VBG differences for pH, PCO2, and bicarbonates were not clinically important for between-person heterogeneity. CONCLUSION: Peripheral venous pH, PCO2, bicarbonates, and total CO2 may be used as alternatives to their arterial equivalents in many clinical contexts encountered in the ICU.
Bicarbonates ; Blood Gas Analysis ; Humans ; Hydrogen-Ion Concentration ; Critical Care ; Intensive Care Units ; Prospective Studies

Eight infants or children with congenital heart disease underwent coorrective cardiac surgery suing hypothemia, cardioulmminary bypass, and total circulatory arrest. Threr were no signisifants change in pH, PaiP2, and arterial bicarbonate ion concentraion between pre-arrest and post-arrest times.
Bicarbonates ; Blood Gas Analysis* ; Child ; Heart Defects, Congenital ; Heart* ; Humans ; Hydrogen-Ion Concentration ; Hypothermia* ; Infant ; Thoracic Surgery*

Eight infants or children with congenital heart disease underwent coorrective cardiac surgery suing hypothemia, cardioulmminary bypass, and total circulatory arrest. Threr were no signisifants change in pH, PaiP2, and arterial bicarbonate ion concentraion between pre-arrest and post-arrest times.
Bicarbonates ; Blood Gas Analysis* ; Child ; Heart Defects, Congenital ; Heart* ; Humans ; Hydrogen-Ion Concentration ; Hypothermia* ; Infant ; Thoracic Surgery*

No abstract available.
Amylases/analysis ; Bicarbonates/analysis* ; Chronic Disease ; Endoscopy, Digestive System ; Human ; Pancreas/physiology* ; Pancreatic Juice/secretion ; Pancreatic Juice/enzymology ; Pancreatic Juice/chemistry* ; Pancreatitis/metabolism ; Pancreatitis/diagnosis*

OBJECTIVETo demonstrate the molecular expression of carbonic anhydrase IV (CA IV) in rabbit corneal endothelium.

METHODSReverse transcriptase polymerase chain reaction (RT-PCR) was performed using cultured and fresh rabbit corneal endothelial total RNA and specific primers for CA IV. The RT-PCR product was subcloned and sequenced. Immunoblotting and indirect immunofluorescence staining were performed to detect protein expression and distribution of CA IV using fresh and cultured rabbit corneal endothelium and rat anti-CA IV polyclonal antibody.

RESULTSRT-PCR screening gave positive bands at the predicted size for CA IV from fresh and cultured rabbit corneal endothelium. Sequencing further confirmed the identity of CA IV in corneal endothelium. Immunoblotting analysis showed a single band at 52 kDa for freshly isolated and cultured endothelial cells. Indirect immunofluorescence staining revealed an apparent positive staining in cultured endothelial cells.

CONCLUSIONCarbonic anhydrase IV is expressed in rabbit corneal endothelium, which could contribute to the transendothelial HCO(3)(-) flux that is necessary to maintain corneal hydration and transparency.

Animals ; Base Sequence ; Bicarbonates ; metabolism ; Carbonic Anhydrase IV ; analysis ; genetics ; Cells, Cultured ; Endothelium, Corneal ; cytology ; enzymology ; Fluorescent Antibody Technique, Indirect ; Molecular Sequence Data ; RNA, Messenger ; analysis ; Rabbits

The effects of epidural anesthesia for normal vaginal delivery on maternal cardiovascular system and boood gas analysis, and newborn umbilical arterial blood gas analysis and Apgar scores were studied in 24 paturients. The paturients were divided into two groups by random sampling, and one group underwent epidurial anesthesia and the other did not. Maternal systolie pressure, heart rate and cardiac output were measured at the time of 4 cm of cervical dilation (preanesthetic), 8 cm of cervical dilation, and immediate postpartum. PH, Pco, Po and bicarbonate levels of maternal atrerial blood were measured at the time of 4 cm and 8 cm of cervical dilation. PH, Pco, Po, and bicarbonate levels of newborn umbilical arterial blood were measured immediately after delivery. One minute and 5 minute Apgar scores of newborn were measured. Systolic blood presssures did not change in both groups. In non-epidural group diastolic and mean blood pressures decreased after delivery. In epidural group its slightly increased at the time of 8 cm of cervical dilation and returned to preanesthetic level after delivery. Heart rate and cardiac output also slightly increased at the time of 8 cm of cervical dilatior. and returned to preanesthetic level after delivery. Maternal pH slightly decreased at the time of 8 cm of cervical dilation in epidural group. Pco2 decreased in both groups, but the rate of decrease was smaller in epidural group. Po increased at the time of 8 cm of cervical dilation in epidural group. Bicarbonate ion level did not changed in both groups. Newborn umbilical arterial blood gas analysis and l minute and 5 minute Apgar scores were not different in both group.
Anesthesia ; Anesthesia, Epidural* ; Bicarbonates ; Blood Gas Analysis ; Cardiac Output ; Cardiovascular System ; Heart Rate ; Humans ; Hydrogen-Ion Concentration ; Infant, Newborn* ; Mothers* ; Postpartum Period