This essay is to present an improvement on the concentration assay for sodium chloride in bicarbonate dialysate.
Bicarbonates ; analysis ; chemistry ; Dialysis Solutions ; analysis ; chemistry ; Sodium Chloride ; analysis

During the routine tests, it's found that the testing method related to Hemodialysis mentioned in the industry standard YY0598-2006 cannot reflect the real bicarbonate concentration in Hemodialysis. To discuss the reaction principle and its interference factors deeply by studying the assay determination of bicarbonate in Hemodialysis, and it could be cited as reference for amending the quality standard or troubleshooting. The improved way of determining bicarbonate concentration in Hemodialysis is also put up.
Bicarbonates ; analysis ; Dialysis Solutions ; analysis ; standards ; Renal Dialysis ; methods ; standards

Orthogonal combination design was adopted in examining the Spirulina platensis (S. platensis) yield and the influence of four factors (Se content, Se-adding method, S content and NaHCO3 content) on algae growth. The results showed that Se content, Se-adding method and NaHCO3 content were key factors in cultivation conditions of Se-enriched S. platensis with the optimal combination being Se at 300 mg/L, Se-adding amount equally divided into three times and NaHCO3 at 16.8 g/L. Algae yield had a remarkable correlation with OD560 and floating rate by linear regression analysis. There was a corresponding relationship between effects of the four factors on algae yield and on OD560, floating rate too. In conclusion, OD560 and floating rate could be served as yield-forming factors.
Bicarbonates ; analysis ; Culture Media ; Cyanobacteria ; growth & development ; Selenium ; analysis ; pharmacology

BACKGROUND: The objective of this study was to examine the correlation between arterial blood gas (ABG) and peripheral venous blood gas (VBG) samples for all commonly used parameters in patients admitted to a medical intensive care unit (ICU). METHODS: A single-center, prospective trial was carried out in a medical ICU in order to determine the level of correlation of ABG and peripheral VBG measurements. A maximum of five paired ABG-VBG samples were obtained per patient to prevent a single patient from dominating the data set. RESULTS: Regression equations were derived to predict arterial values from venous values as follows: arterial pH=-1.108+1.145xvenous pH+0.008xPCO2-0.012xvenous HCO3+0.002xvenous total CO2 (R2=0.655), arterial PCO2=88.6-10.888xvenous pH+0.150xPCO2+0.812xvenous HCO3+0.124xvenous total CO2 (R2=0.609), arterial HCO3=-89.266+12.677xvenous pH+0.042xPCO2+0.675xvenous HCO3+0.185xvenous total CO2 (R2=0.782). The mean ABG minus peripheral VBG differences for pH, PCO2, and bicarbonates were not clinically important for between-person heterogeneity. CONCLUSION: Peripheral venous pH, PCO2, bicarbonates, and total CO2 may be used as alternatives to their arterial equivalents in many clinical contexts encountered in the ICU.
Bicarbonates ; Blood Gas Analysis ; Humans ; Hydrogen-Ion Concentration ; Critical Care ; Intensive Care Units ; Prospective Studies

BACKGROUND: The objective of this study was to examine the correlation between arterial blood gas (ABG) and peripheral venous blood gas (VBG) samples for all commonly used parameters in patients admitted to a medical intensive care unit (ICU). METHODS: A single-center, prospective trial was carried out in a medical ICU in order to determine the level of correlation of ABG and peripheral VBG measurements. A maximum of five paired ABG-VBG samples were obtained per patient to prevent a single patient from dominating the data set. RESULTS: Regression equations were derived to predict arterial values from venous values as follows: arterial pH=-1.108+1.145xvenous pH+0.008xPCO2-0.012xvenous HCO3+0.002xvenous total CO2 (R2=0.655), arterial PCO2=88.6-10.888xvenous pH+0.150xPCO2+0.812xvenous HCO3+0.124xvenous total CO2 (R2=0.609), arterial HCO3=-89.266+12.677xvenous pH+0.042xPCO2+0.675xvenous HCO3+0.185xvenous total CO2 (R2=0.782). The mean ABG minus peripheral VBG differences for pH, PCO2, and bicarbonates were not clinically important for between-person heterogeneity. CONCLUSION: Peripheral venous pH, PCO2, bicarbonates, and total CO2 may be used as alternatives to their arterial equivalents in many clinical contexts encountered in the ICU.
Bicarbonates ; Blood Gas Analysis ; Humans ; Hydrogen-Ion Concentration ; Critical Care ; Intensive Care Units ; Prospective Studies

Eight infants or children with congenital heart disease underwent coorrective cardiac surgery suing hypothemia, cardioulmminary bypass, and total circulatory arrest. Threr were no signisifants change in pH, PaiP2, and arterial bicarbonate ion concentraion between pre-arrest and post-arrest times.
Bicarbonates ; Blood Gas Analysis* ; Child ; Heart Defects, Congenital ; Heart* ; Humans ; Hydrogen-Ion Concentration ; Hypothermia* ; Infant ; Thoracic Surgery*

Eight infants or children with congenital heart disease underwent coorrective cardiac surgery suing hypothemia, cardioulmminary bypass, and total circulatory arrest. Threr were no signisifants change in pH, PaiP2, and arterial bicarbonate ion concentraion between pre-arrest and post-arrest times.
Bicarbonates ; Blood Gas Analysis* ; Child ; Heart Defects, Congenital ; Heart* ; Humans ; Hydrogen-Ion Concentration ; Hypothermia* ; Infant ; Thoracic Surgery*

No abstract available.
Amylases/analysis ; Bicarbonates/analysis* ; Chronic Disease ; Endoscopy, Digestive System ; Human ; Pancreas/physiology* ; Pancreatic Juice/secretion ; Pancreatic Juice/enzymology ; Pancreatic Juice/chemistry* ; Pancreatitis/metabolism ; Pancreatitis/diagnosis*

OBJECTIVETo demonstrate the molecular expression of carbonic anhydrase IV (CA IV) in rabbit corneal endothelium.

METHODSReverse transcriptase polymerase chain reaction (RT-PCR) was performed using cultured and fresh rabbit corneal endothelial total RNA and specific primers for CA IV. The RT-PCR product was subcloned and sequenced. Immunoblotting and indirect immunofluorescence staining were performed to detect protein expression and distribution of CA IV using fresh and cultured rabbit corneal endothelium and rat anti-CA IV polyclonal antibody.

RESULTSRT-PCR screening gave positive bands at the predicted size for CA IV from fresh and cultured rabbit corneal endothelium. Sequencing further confirmed the identity of CA IV in corneal endothelium. Immunoblotting analysis showed a single band at 52 kDa for freshly isolated and cultured endothelial cells. Indirect immunofluorescence staining revealed an apparent positive staining in cultured endothelial cells.

CONCLUSIONCarbonic anhydrase IV is expressed in rabbit corneal endothelium, which could contribute to the transendothelial HCO(3)(-) flux that is necessary to maintain corneal hydration and transparency.

Animals ; Base Sequence ; Bicarbonates ; metabolism ; Carbonic Anhydrase IV ; analysis ; genetics ; Cells, Cultured ; Endothelium, Corneal ; cytology ; enzymology ; Fluorescent Antibody Technique, Indirect ; Molecular Sequence Data ; RNA, Messenger ; analysis ; Rabbits

PURPOSE: The aim of this study was to analyze the risk factors of systemic complications by caustic substances according to arterial blood gas analysis (ABGA). METHODS: The medical records of patients who visited our emergency department for caustic ingestion from January 2000 to December 2011 were reviewed. There were 129 patients included in this study, with a mean age of 45.4 years, and 46.9% of the patients were men. We performed a univariate analysis of factors associated with systemic complication and a logistic regression analysis of these predictive factors. RESULTS: The most frequent caustic ingested was base (53.8%). Systemic complications were found in 29 patients (22.5%) and advanced age, a low partial pressure of oxygen (PaO2), low bicarbonate ion (HCO3-), low oxygen saturation (SaO2), high anion gap (AG), acid ingestion, and severe acidosis were associated with systemic complications. Low SaO2, high AG, and severe acidosis were independent predictive factors of systemic complications. ED: HIGHLIGHT: Please spell out HCO3. CONCLUSION: Parameters of ABGA such as SaO2, AG, and pH predict the development of systemic complications by caustic ingestion. Therefore, these data have a role in the prognosis and treatment of caustic ingestion.
Acid-Base Equilibrium ; Acidosis ; Bicarbonates ; Blood Gas Analysis ; Caustics ; Eating ; Emergencies ; Humans ; Hydrogen-Ion Concentration ; Logistic Models ; Male ; Medical Records ; Oxygen ; Partial Pressure ; Prognosis ; Risk Factors