Carotid Stenosis ; surgery ; Endarterectomy, Carotid ; Humans

Diagnosis, Differential ; Humans ; Medicine, Chinese Traditional

Objective To explore the quality of life (QoL) among the female condyloma acuminatum patients.Methods 118 female condyloma acuminatum patients were interviewed face-to-face by the well-trained nurses with questionnaire including SF-36 Chinese version,the t-test and x2 test were used to compare the scale scores of SF36 between the patient and control groups.Results There were 101 eligible questionnaire,the scale scores of physical function(PF) and bodily pain(BP) were highest in the patient group,the scale score was (92.9 ± 10.5)points and (75.0 ± 1 1.2) points,respectively.Role limitations due to emotional problems (RE) was the lowest with the scale score only(50.8 ± 13.7)points.All scale scores of SF-36 in patient group,except PF(no statistical significant),were lower than that in the control group (all P ＜ 0.01).Conclusion In the female condyloma acuminatum patients,the QoL is lower significantly than that in the control group,especially in emotional scales.

Paralytic shellfish poisoning contamination is a threat to human health. Based on review of research articles pertaining to paralytic shellfish poisoning contamination in shellfishes and assessment of dietary exposure to paralytic shellfish poisoning in coastal cities of China from 2007 to 2022, the article describes the status of paralytic shellfish poisoning contamination, influencing factors and dietary exposure assessment in different sea areas of China (Bohai Sea, Huanghai Sea, East China Sea and South China Sea), and the relatively high-level contamination of paralytic shellfish poisoning in shellfishes is found to strongly correlate with red tides caused by season and water temperature in the Bohai Sea and South China Sea areas. Acute exposure assessment based on point estimate model is commonly used for assessment of dietary exposure to paralytic shellfish poisoning, and the risk of human dietary exposure to paralytic shellfish poisoning is within the acceptable range in most areas. Intensified monitoring of paralytic shellfish poisoning contamination in shellfishes and full consideration of parameters like processing factor during dietary exposure assessment are required in the future to allow more precise results.

Peanuts, corn and other food products are prone to aflatoxins (AF). AF was listed as a human carcinogen by the International Agency for Research on Cancer (IARC), especially aflatoxin B1 (AFB1), as it is considered the most prevalent and toxic. China is a big peanut producer, so carrying out pollution investigation and risk assessment in peanuts and their products is crucial to formulate prevention measures, protect export trade and maintain health. This paper summarizes the AF pollution of peanuts and their products in the Huang-Huai-Hai Basin, Yangtze River Basin, Southeast Coast and Northeast region of China from 2015 to 2021, and the application of dietary AF exposure risk assessment methods, providing a basis for strengthening the supervision of AF pollution in peanuts and their products, and ensuring food safety.

Abstract Zearalenone (ZEN), a nonsteroidal mycotoxin produced by fusarium with estrogen-like effects, and it contaminates various types of cereals and their products to varying degrees. Wheat and rice are the staple foods of Chinese residents, and understanding the contamination status and dietary exposure risk of ZEN in grains and their products is of great significance for maintaining food safety and promoting population health. This article reviewed the publications between 2012 and 2023 on ZEN contamination and dietary exposure assessment in cereals and their products, and found that the dietary exposure risk of ZEN contamination in cereals and their products was low. Continued efforts are needed to strengthen food safety monitoring and regulatory oversight to ensure food safety.

Acute Disease ; Adolescent ; Adult ; Aluminum Compounds ; poisoning ; Child ; Female ; Humans ; Male ; Middle Aged ; Pesticides ; poisoning ; Phosphines ; poisoning ; Poisoning ; diagnosis ; therapy

History, 20th Century ; History, 21st Century ; Humans ; Minimally Invasive Surgical Procedures ; history ; trends ; Neurosurgery ; history ; trends

Objective To explore the therapeutic effects of human glial cell line-derived neurotrophic factor (GDNF)-engineered rat neural stem cell (NSC) transplantation in rat model of Parkinson's disease ( PD) . Methods SD rats received a single injection of 24 μg of 6-hydroxydopamine (6-OHDA) at two sites in right striatum. Then 10 days after surgery, the successful animal models of PD were divided into 3 groups: PD model group ( 2 μl transplantation media was injected in right striatum), NSC group (transplanted were 2×10~5 NSCs infected by bare lentivirus) and GDNF group (transplanted were 2×10~5 GDNF-engineered NSCs). The rotation scores were assessed 5 weeks, 7 weeks and 9 weeks after transplantation. The dopaminergic neurons in substantia nigra ( SN ) were analyzed quantitatively using immunohistochemistry for tyrosine hydroxylase (TH), and the dopamine and its metabolites, dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) were analyzed 9 weeks after transplantation by high performance liquid chromatography ( HPLC) . Results GDNF-engineered NSC transplantation could effectively improve the behavioral performance in rats. At the 5th week after cell transplantation, the rotation turns within 90 min were (993. 9±159. 1) turns, (956. 7±136. 3) turns and (433. 6±100. 9) turns in PD model group, NSC group and GDNF group respectively (F=95. 694, P = 0. 000). At the 7th week, the rotation turns within 90 min were (964. 2 ± 152.0) turns, (909. 2 ± 136. 3) turns and (399. 4±84. 4) turns in PD model group, NSC group and GDNF group respectively (F = 106. 134, P=0. 000). At the 9th week, the rotation turns within 90 min were (909. 5±152. 2) turns, (865. 5± 129. 1) turns and (312. 2±63. 7) turns in PD model group, NSC group and GDNF group respectively (F= 151. 100, P = 0.000). GDNF-engineered NSC transplantation could significantly increase the levels of dopamine and its metabolites in injured striatum. The concentrations of dopamine in injured striatum was higher in GDNF group than that in PD model group and NSC group C(7. 5±0. 8) ng/mg vs. (3.3±0.3) ng/mg and (3. 7±1. 3) ng/mg, F=59. 543, P = 0. 0003. The level of DOPAC was higher in GDNF group than that in PD model group and NSC group C(0. 9±0. 1) ng/mg vs. (0. 5± 0. 1) ng/mg and (0. 6±0. 2) ng/mg, F= 17. 293, P=0. 000]. The concentration of HVA in injured striatum was higher in GDNF group than that in PD model group and NSC group [(0. 9±0. 1) ng/mg vs. (0.5±0. 1) ng/mg and (0. 6±0. 2) ng/mg, F=35.175, P = 0.000]. Conclusions engineered NSC transplantation improves the function of dopamine system in SN and striatum, and GDNF gene therapy has potential clinical value.

Objective To knock down hemolysin hlyA gene and insert green fluorescence protein gene of vibrio cholerae vaccine candidate NFYY101. Methods Amplified fragments of hlyA gene upstream (hlyAup) and downstream (hlyAdown),lacz-GFPuv,and hlyAup-laczGFPuv-hlyAdown, and plasmids treated with specific enzymes were utilized to construct recombinant plasmids pUC18-hlyAup-laczGFPuv-hlyAdown and pCVD442-hlyAup-laczGFPuv-hlyAdown. Following the construction of the recombinant suicide plasmids ,a vaccine candidate was constructed by homologous recombination ,while SM10λpir carrying pCVD442- hlyAup-laczGFPuv-hlyAdown was utilized as the donor strain to transfect NFYY101. Results Construction of recombinant suicide plasmids pCVD442- hlyAup-laczGFPuv-hlyAdown was satisfactory that hemolysin hlyA gene was knocked out and green fluorescence protein gene was successfully inserted of vibrio cholerae vaccine candidate NFYY101. Conclusion Construction of the recombinant suicide plasmid pCVD442-hlyAup-laczGFPuv-hlyAdown successfully can be used for knocked out the hlyA gene and added green fluorescence protein gene as genetic marker into the chromosomal DNA of vibrio cholerae vaccine candidate.