Adult ; Aged ; Female ; Fistula ; surgery ; Humans ; Male ; Middle Aged ; Pectoralis Muscles ; transplantation ; Pharyngeal Diseases ; surgery ; Reconstructive Surgical Procedures ; methods ; Surgical Flaps

Objective To observe the relationship between homocysteine (Hcy),C-reactive protein (CRP) and type 2 diabetes peripheral vascular disease (PVD).Methods Fifty-eight patients with type 2 diabetes according to the condition of PVD were divided into simple type 2 diabetes mellitus group (DM group,30 cases) and PVD group (28 cases).Twenty-five healthy people were chosen at the same period as control group.The fasting blood glucose (FBG),glycosylated hemoglobin (HbA1c),total cholesterol(TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C),Hcy and CRP levels were detected among 3 groups,and the body mass index (BMI),ankle brachial index(ABI) was calculated.Results The BMI,TG,TC and HDL-C levels among 3 groups had no statistical significance (P ＞0.05).The FBG,HbA1c and LDL-C in DM group and PVD group were significantly higher than those in control group [(7.54 ± 1.25),(8.12 ± 1.57) mmol/L vs.(4.68 ±0.54)mmol/L; (6.92 ± 0.89)％,(7.54 ± 0.97)％ vs.(4.80 ± 0.54)％ ; (3.79 ± 0.73),(4.41 ± 0.93) mmol/L vs.(2.68 ± 0.54) mmol/L],and the difference was statistically significant (P ＜ 0.05).But the difference between PVD group and DM group had no statistical significance (P ＞ 0.05).Hcy and CRP in DM group and PVD group were higher than those in control group [(10.26 ± 3.62),(19.83 ± 5.54) μ mol/L vs.(8.92 ± 2.41)μ mol/L; (4.12 ±0.57),(5.47 ±0.85) mg/L vs.(2.73 ±0.54) mg/L],ABI was lower than that in control group(0.92 ± 0.18,0.81 ± 0.16 vs.1.02 ± 0.23),and the difference was statistically significant (P ＜ 0.05);Hcy,CRP in PVD group were obviously higher than those in DM group,ABI was lower than that in DM group,and the differences were statistically significant (P＜ 0.05).Spearman analysis showed that Hcy was negatively correlated with ABI (r =-0.635,P ＜ 0.05),CRP was negatively correlated with ABI (r =-0.529,P ＜ 0.05),and Hcy was positively correlated with CRP (r =0.528,P ＜ 0.05).The partial correlation analysis results showed that Hcy and ABI still showed a negative correlation (r =-0.315,P ＜ 0.05),CRP and ABI still showed a negative correlation (r =-0.243,P ＜ 0.05).Conclusion Hcy and CRP participating in the occurrence and development of type 2 diabetes PVD,can be used as a predictor of type 2 diabetes PVD.

Air ; analysis ; Air Pollutants, Occupational ; analysis ; Chromatography, Gas ; methods ; Hydrocarbons, Chlorinated ; analysis

Objective To improve the understanding of tuberculosis X-ray performance at Linzhi region in the east of Tibet,in order to better guide clinical early diagnosis.Methods The X -ray image characteristics of 30 patients with pulmonary tuberculosis from Linzhi region in the east of Tibet were retrospectively analyed,Original they all took chest plain films at initial diagnosis.Results The original hairstyle tuberculosis was not found;Blood type disseminated tuberculosis in 5 cases (16%).Composition of mixed density shadow image performance,calcification and satellite stove were founded;Invasion tuberculosis were in 8 cases (26%),images showed two pulmonary diffuse distribution of nodular shadows;Hollow fiber tuberculosis were in 17 cases (58%),images showed empty,fibrosis, endobronchial spread of three characteristics,and lesions was wide,especially with effusion infiltration priority,which were distributed in the lung base and at the front.Conclusion Plateau pulmonary tuberculosis with invasion and fiber cavity is given priority to,and have a specific image characteristics,it can be used for diagnosis.And its X -ray per-formance are significantly different from that of mainland cities.

Objective To explore the application value in primary hospitals using conventional ultrasound and ultrasonic elastography in the diagnosis and surgery of breast cancer both in the diagnosis of joint.Methods One hundred and sixteen patients with breast disease confirmed by surgical pathology were studied retrospectively .All the patients were examined by acoustic elastogra-phy and conventional ultrasonography before surgery.Results Pathological results of 116cases were:74 malignanies and 42 benigni-ties.①Preoperative routine ultrasonography in diagnosis of benign breast lesions in 41 cases, 65 cases of malignant lesions.Ultra-sonography showed that benign lesions were round or oval, smooth edge, no obvious signs of malignant microcalcifications, breast cancer spiculation (87.84%, 65/74), intralesional calcification (62.16%, 46/74).With the pathology of routine preoperative ul-trasound diagnosis coincidence rate was 91.38%(106/116).②Ultrasonic elastography in diagnosis of breast benign lesions in 39 ca-ses, 66 cases of malignant lesions, benign lesions of ultrasound elastography score of 1-2 (92.86%, 39/42); malignant lesions of ultrasound elastography score of 3-5 (89.19%, 66/74);and the pathology of preoperative ultrasound elastography diagnosis coinci-dence rate was 90.51% (105/116).③Preoperative ultrasound elastography and conventional ultrasound diagnosis of 42 cases of be-nign lesions, 68 cases of malignant lesions, and pathological diagnosis coincidence rate was 94.82% (110/116).Conclusion Ul-trasound elastography and conventional ultrasound examination can improve the resection of benign and malignant breast lesions diag-nosis in primary hospital diagnosis, as an effective method for the detection of breast cancer before operation .

Objective To investigate the effect of implanting uncovered self-expandable metal stent for treatment of distal malignant biliary obstruction through endoscope.Methods The effect of therapy about implanting uncovered self-expandable metal stents to 16 patients who had unsectable malignant tumors companing with obstructive jaundice through endoscope was reviewed. Results Fifteen of the studied patients were implanted uncovered self-expandable metal stents successfully (94%), for their internal drainage were patent. At the seventh and fourteenth day after implantation, liver function and B-ultrasound were rechecked. Compared to the data before operation, total bilirubin, direct bilirubin and transaminase declined respectively (P

[Objective] To observe the curative effect of integrated Traditional Chinese Medicine and western medicine on the disease of cystic hyperplasia of breast.[Method] The 214 cystic hyperplasia of breast cases were randomly divided into two groups.125 cases in the integrated Traditional Chinese Medicine and western medicine group(observed group).Control group(89 cases)were treated in common practiced western medicine.[Result]In observed group,70 cases fully recovered from illness,40 cases were excellent,6 cases were effective,9case were ineffective.Total effective rate was 92.8%.In control group,27 cases were fully recovered from illness,22 cases were excellent,20 cases were effective,9 cases were ineffective.Total effective rate was 77.4%.The differences between two groups were significant(P

OBJECTIVE:To study the effects of carbamylated erythropoietin(CEPO)on cardiovascular microcirculation in rats with diabetes mellitus. METHODS:Rats were randomly divided into blank control group,model group,CEPO low-dose,medi-um-dose and high-dose groups(500,1 000,2 000 u/kg)with 12 in each group. The rats in the last 4 groups were reduced diabetes mellitus model. All rats were given relevant medicine intragastrically twice a week,coronary microcirculation endothelial cells were separated after consecutive 4 weeks. Enzyme-linked immunosorbent assay was conducted to detect levels of peripheral serum prosta-cyclin (PGI2),vasoconstrictor endothelin-1 (ET-1),angiotensin Ⅱ(AngⅡ) and von Willebrand factor (vWF) of rats in each group;in vitro CCK 8 test was used to detect endothelial cell activity(OD value);real-time quantitative polymerase chain reaction was adopted to detect proliferation-related genes(Ki67,p16),poptosis-related genes(Bad,Bax),and expressions of protein vas-cular endothelial growth factor(VEGF)and AngⅠ. RESULTS:Compared with blank control group,levels of PGI2,ET-1,AngⅡand vWF in serum in model group increased;OD value deceased;Ki67,p16,Bax and VEGF expression decreased;the difference was statistically significant (P<0.05). Compared with model group,levels of PGI2,ET-1,AngⅡ and vWF in serum in CEPO low-dose,medium-dose and high-dose groups increased;OD value increased;Ki67,p16 and VEGF expression increased;expres-sions of Bad and Bax decreased;the difference was statistically significant(P<0.05). The others had no significant difference(P>0.05). CONCLUSIONS:CEPO maybe improve the coronary microcirculation function by upregulating VEGF expression in coro-nary microcirculation endothelial cells and promoting endothelial cells’regeneration.

Objective To establish a method for determination of total hardness in drinking water by using automatic potentiometric titrator.Methods Dynamic equivalence point titration(DET)and monotonic equivalence point titration(MET)mode was used to determine the high total hardness and low total hardness of drinking water samples respectively.Results Used DET mode to determine the high total hardness,the relative standard deviation(RSD)was 0.69%～1.72% and the recovery rate was 101.5%～102.2%.Used MET mode to determine the low total hardness,RSD was 3.49%～4.00% and the recovery rate was 95.8%- 103.6%.Conclusion This method is rapid,simple,accurate,precise and applicable to the determination of total hardness in drinking water in low and high levels.

Bacterial Proteins ; pharmacology ; Blotting, Western ; Humans ; Immunohistochemistry ; methods ; Interleukin-8 ; genetics ; metabolism ; Internal Fixators ; Lung ; cytology ; drug effects ; Lung Diseases ; microbiology ; Pharmaceutical Solutions ; pharmacology ; Porins ; pharmacology ; RNA, Messenger ; analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Solutions ; chemistry