AIM: To study the effects of arsenic trioxide (As_2O_3) on mouse airway fibroblast (FB) proliferation and expression of c-myc and c-sis in cultured FB. METHODS: The cultured FB was divided into seven groups. NS, dexamethasone (1.0 ?mol/L) and As_2O_3 at concentrations of 0.25, 0.5, 1.0, 2.0, 4.0 ?mol/L were instilled respectively into cultured FB. The effects of different concentration of As_2O_3 and dexamethasone on cell growth were observed at 1, 3, 5 and 7 day. The changes of cell cycle and the positive expression rate of c-myc and c-sis were examined by flow cytometry (FCM). RESULTS: Various concentrations of As_2O_3 significantly inhibited the proliferation of FB in vitro and showed a dose-dependent and time-dependent tendency. Data from FCM indicated that G_1-phase cell percentage increased and G_2/M-phase cell percentage decreased with the increase in As_2O_3 concentrations. The expression of c-myc and c-sis was significantly inhibited by As_2O_3 (2.0, 4.0 ?mol/L). CONCLUSION: As_2O_3 inhibits FB proliferation by downregulating the expression of c-myc and c-sis.

AIM: To investigate the changes of T cell cycle, the expression of bcl-2 in allergic asthmatic mice and the effects of dexamethasone on them. METHODS: An animal model with asthma was established by means of ovalbumin sensitizing-challenging. CD3 expression in spleen and lymphocytes in bronchoalveolar lavage fluid (BALF), T cell cycle and Bcl-2 expression in spleen were detected by flow cytometry. RESULTS: In BALF lymphocytes and spleen lymphocytes, CD3 expression rate in the asthmatic group was significantly higher than that of control group. In BALF lymphocytes, CD3 expression rate in the asthma plus dexamethasone group was significantly lower than that of the asthmatic group. However, in spleen lymphocytes, CD3 expression rate in the asthma plus dexamethasone group was significantly higher than that of the asthmatic group. In spleen lymphocytes, the cell count in S phase, G 2+M phase and apoptosis rate of T cell from the asthmatic group were significantly higher than that from the control group. Cell count in S phase, G 2+M phase and apoptosis rate of T cell from the asthmaplus dexamethasone group was significantly lower than that from the asthmatic group. The Bcl-2 expression rate of T cell from the asthmatic group was significantly higher than that from the control group. CONCLUSIONS: In the allergic asthmatic mice model, T cell count, proliferation and activation of T cells, apoptosis rate of T cells in spleen lymphocytes increase, meanwhile bcl-2 expression also increases significantly. There was no significant effect of dexamethasone on the bcl-2 expression. The therapeutic effects of dexamethasone on asthma may be not due to the inhibition of the bcl-2 expression in T cells.

Objective To study the feasibility of cerebral perfusion weighted imaging(PWI)using superparamagnetic iron oxide particles(SPIO)Resovist in normal rabbits,and investigate the methodology and the proper contrast agent dose of SPIO in PWI.Methods All 30 rabbits were randomly divide into 5 groups(n=6 for each),group A,B,C,D were divided according to the different dose of Resovist(4,8,16 and 32 μmol Fe/kg)and group E was operated as control study with 0.2 mmol/kg Gd-DTPA.The dynamic MR perfusion imaging series were acquired in each group.The signal intensity time curves were analyzed in gray matter and white matter,and the parameters(rCBV,SRRmax,QrCBV,QSRR max)were calculated and compared in all groups and correlated with control one.Results a bolus injection was succeed for all studies.and a dose-dependent strong reduction in signal intensity of gray and white matter in all groups was demonstrated.The rCBV values of gray and white matter for group A,B,C,D and E were(50.48±3.84),(25.57±2.10);(94.69±2.60),(45.33±3.14);(141.13±6.26),(67.67±4.65);(243.75±5.90),(162.06±5.14);and(84.60±3.60),(41.36±2.18)ml/100 g,respectively.The SRRmax values of gray and white matter for group A,B,C,D and E were(13.70±1.50)%,(7.38±0.41)%;(31.01±4.06)%,(16.49±2.35)%;(43.81±3.42)%,(21.64±4.14)%;(64.49±5.35)%,(43.61±5.78)%;and(27.78±2.98)%,(14.42±2.25)%;respectively.The QrCBV values for group A,B,C,D and E were 1.98±0.07,2.09±0.11,2.09±0.07,1.50±0.01,and 2.05±0.03,respectively,and the QSRR max values for group A,B,C,D and E were 1.85±0.11,1.88±0.06,2.06±0.25,1.49±0.09,and 1.94±0.12,respectively.All above values among group were significantly different(P＜0.01).For the QrCBV and QSRR max values between group,there were no significant difference between group A and B,B and C,A and C;while the values of group A was significantly larger than those of group D(P＜0.01).Condusion Resovist(SPIO)can be used in the study of MR perfusion weighted imaging in the brain,and the proper contrast agent dose is 4-16 μmol Fe/kg.

Objective　To study the clinical application of MRI in neonatal asphyxia.Methods　There were twenty-two cases of full term infants(≤one year old),they all had asphyxia during delivery.Transverse section on TSE T2-weighted,TSE T1-weighted and FLAIR sequences,and sagittal section on TSE T2-weighted were obtained on MRI.Results　The abnormal signal intensity in white matter on MRI were found in 15 cases.The volume of white matter was decreased in three cases.Subarachnoid spaces of frontal and temporal lobe were enlarged in two cases.Two cases were normal on MRI.Conclusion　We believed that the changes of the white matter of brain in neonatal asphyxia can be showed on MRI,it is of important significance for clinical diagnosis and treatment.

Purpose:To investigate effect on bcl-2 and bax expression in apoptic PGCl_(3) cells and to study the mechanism of apoptosis of PGCl_(3) cells induced by death associated protein kinase(DAPK).Methods:Eukaryotic express vector pcDNA3.1-DAPK was tranfected into High-metastasis Non-small Lung Cancer Cell PGCl_(3).Changes of bcl2 and bax expression were detected with RT-PCR.Phospharation of Bcl-2 was examined with Westernblotting.Results:It was demonstrated that bcl-2 expression was down regulated,but bax expression did not change in apoptosis of PGCl_(3) cells induced by DAPK.Level of Phospharation of Bcl-2 increased.Conclusions:Apoptosis of PGCl_(3) cells induced by over-expression of DAPK may be associated with down regulating of bcl-2 expressio and increase of phospharation of Bcl-2.

AIM: To investigate the effects of oral administration of Astragalus membranceus, mung bean and arsenolite on the toxic of the arsenolite induced rats and the possible mechanisms with metallothionein (MT). METHODS: All the rats were oral administration with arsenolite. The Astragalus membranceus and mung bean were compared with the cadmium chloride which induced MT synthesis. The contents of MT were determined by cadmium saturation method, the liver mRNA levels for MT 1, MT 2 were detected by RT PCR. The protective effects of renal and liver were observed by testing alanine aminotransferase (ALT), blood urea nitrogen (BUN) and serum creatinine (SCR). RESULTS: The contents of MT were accorded with the mRNA expression of MT 1, MT 2. Arsenolite, Astragalus membranceus and mung bean could induce the synthesis of MT, but the contents of MT which arsenolite induced were trace. The contents of MT significantly increased after oral administration of Astragalus membranceus and mung bean, especially in the Astragalus membranceus group (P

As inhibitors of osteoclasts, Bisphosphonates are a group of drugs used in the treatment of oncology patients with bone metastases. However, some reports have described osteonecrosis of the jaws occurring after taking drugs containing bisphosphonates. The advances in clinical manifestation, imaging findings as well as the mechanisms of bisphosphonate-associated osteonecrosis of the jaws were reviewed in this article.

Objective To study the epidemiological features of patients for emergency transport so as to enhance the control level of pre hospital emergency treatment. Methods Investigations were made of 956 cases of patients for emergency transport in the authors hospital in 2000～2001 and their epidemiological features analyzed. Results Among the 956 cases, males outnumbered females, patients within the age group of 20 to 39 numbered 391, accounting for 40.9%, and patients within the age group of 50 to 69 numbered 400, accounting for 41.8%. Of all the cases, ordinary ones numbered 671(70.2%),critical ones numbered 241(25.2%), and cases that died numbered 44(4.6%). The first three diseases demanding emergency transport were successively craniocerebral wound(17.4%), cerebrovascular disease(16.0%), and cardiovascular disease(13.5%). The periods for emergency transport ranged mainly from 9:00 to 12:00 and from 15:00 to 18:00. The sources of emergency cases were successively township hospitals(53.8%), hospitals at the county level(26.0%), and hospitals at the city level(20.2%). Conclusion Control of pre hospital emergency treatment needs to be strengthened according to the epidemiological features of patients for emergency transport.

Objective To study the clinical characteristics in young women with breast cancer.Methods Clinical data on 71 young breast cancer cases (under 35 years) out of a total 810 female cases during a period of 20 years were retrospectively analyzed. Result Delayed medical consultation was more often seen in young patients(56?4)?d vs (35?3)?d; u=2.33; P

Objective To evaluate the value of Feridex in demonstrating small focal lesions of the liver on MRI and to discuss the optimal scan time after intravenous administration of Feridex. Methods Small focal lesions in the liver were found on ultrasound or CT in 17 patients. Conventional MR plain scan and Gd-DTPA enhanced scan were performed. One to three days later, T 2 weighted imaging was repeated at 0.5 h, 3 h, and 6 h after Feridex was administered intravenously over 30-40 min at a dose of 0.05 ml/kg. The pre- and post-contrast T 2WI were compared and analyzed in terms of lesion detectability and signal change. Results Twenty-one lesions with diameter smaller than 1 cm were found on the Feridex enhanced images, and only 8 lesions were found on the conventional MR images. The small hepatic cancers, focal nodular hyperplasia (FNH), and regenerated nodules had different signal changes on the Feridex images, which made the differential diagnoses easier. The signal of the liver decreased significantly (P0.05). Conclusion Fridex enhanced MR images could not only improve the detection of small focal lesions, but also provide evidence for differential diagnoses.