Fungal keratitis is a corneal disease caused by fungal infection with high possibility of blindness,and the morbidity is being higher year by year.Both native immune and adaptive immune are included during antifungi process in which the fungi is killed by native immune especially by neutrophil.The neutrophil kill the fungi not only by oxygen-dependent and oxygen-independent way but also by the neutrophil extracellular traps which is discovered in recent years.The enzyme and reactive oxygen species(ROS) released by the neutrophil are included in the process of fungi killing.But those materials can cause tissue damage which may cause the scar or even corneal perforation.This review mainly focused on the mechanism how the neutrophil kill fungi and cause tissue damage.

The paper reports a review on the current situation of the hierarchy and quality of the evidence related to both national and international evidence-based nursing research and practice.The limitations and barriers have been analyzed from both research methodology and data acquisition technique aspects.The available evidences provided by past studies are limited in post hoc indicators (e.g.hospital stay,fatigue,stress or sleep quality,etc.) and subjective methodologies (e.g.questionnaire,self-report or interview,etc.),that restrains the further development of evidence-based healthcare,in terms of its reliability,validity and application.The advantages/disadvantages and feasibility of new potential technologies,such as body sensors and smart phones,have also been discussed.

OBJECTIVE:To investigate the correlation of serum resistin and adiponectJn Ievels with bone mineral density(BMD)in 232 healthy male volunteers in Changsha area.METHODS:A totaI of 232 healthy Chinese men of Han population were selected randomly from Changsha area.All subjects gave their informed consent to participate in this study.Subjects were excluded from the study if they had conditions or received treatment that affecting bone metabolism.Serum adiponectin and resistin levels were determined by ELISA.BMD was measured by dual energy X-ray absorptiometry in total body,lumbar spine,and total hip.Body fat mass and lean body mass were measured by the whole body scanning.The correlation of serum adiponectin and resistin levels with fat mass and BMD at various sites were analyzed;the predictors of BMD at various sites was assayed using multiple linear stepwise regression analysis.RESULTS:Adiponectin was negatively correlated with fat mass(r=-0.216.P＜0.05),while resistin had no correlation with fat mass.The correlation disappeared after adjustment for age and fat mass(r=-0.006,P＞0.05).The significant negative correlations between adiponectin and BMD were found,and remained significant after adjustment for age and fat mass.There was no correlation between resistin and BMD at various sites.In the multiple linear stepwise regression analysis.adiponectin was an independent predictor of BMD in men.CONCLUSION:Resistin js not correlated with fat mass and BMD at various sites.Adiponectin is correlated with fat mass,and has a negative correlation with BMD at various sites,it is an independent predictor of BMD in Chinese men.

The activity of eNOS was singificantly depressed by a high concentration of glucose in a concentration-and time-dependent manners after incubation of HUVECs with different concentrations of glucose and with high glucose plus insulin for different times.The physiological concentration of insulin can partially reverse the inhibitions of the activity and expression of eNOS induced by high concentration of glucose.

Objective To evaluate the efficacy of radiofrequeney ablation on tachyrhythmia and cardiac acute injure.Methods Endomyocardin was signified and ablated using routine methods,the effects of radiofrequency ablation on cardiac autonomic nerve function,cardiac systolic function,radiofrequency electric injury and changes of troponin,SOD and endothelin level in pre-operation and post-operation were evaluated to study RFCA procedure caused cardiac acute injury.Results Thirty-eight patients with tachyarrhythmia underwant RFCA.The successful rate was 100%,and none had serious complications.Postoperative LF and HF were reduced significantly as compared with preoperation,Atrium and ventricle premature beats had no significant difference before and after RFCA.Cardiac systolic function had no significant changes before and after procedure,but troponin,SOD and endothelin levels were significantly higher in postoperation than those of preoperation.Conclusions RFCA is a principal therapy of tachyarrhythmia because its excellent therapeutic effect,less side effect and eradication,RFCA has acute cardiac injury,but the injury is slight.

As inhibitors of osteoclasts, Bisphosphonates are a group of drugs used in the treatment of oncology patients with bone metastases. However, some reports have described osteonecrosis of the jaws occurring after taking drugs containing bisphosphonates. The advances in clinical manifestation, imaging findings as well as the mechanisms of bisphosphonate-associated osteonecrosis of the jaws were reviewed in this article.

Adolescent ; Adult ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Cytarabine ; therapeutic use ; Female ; Granulocyte Colony-Stimulating Factor ; therapeutic use ; Humans ; Leukemia, Myeloid, Acute ; drug therapy ; Male ; Middle Aged ; Treatment Outcome ; Vidarabine ; analogs & derivatives ; therapeutic use ; Young Adult

Air ; analysis ; Air Pollutants, Occupational ; analysis ; Chromatography, Gas ; methods ; Hydrocarbons, Chlorinated ; analysis

Objective To explore the clinical effect of autologous platelet-rich plasma ( PRP) combined with closed reduction and hol-low screw internal fixation for femoral neck fracture .Methods Totally 200 cases of femoral neck fracture were collected from May 2010 to August 2014 in our hospital .Randomly divided them into two groups , namely the PRP group and the control group , with 100 patients in each group.The PRP group were given autologous platelet-rich plasma ( PRP) combined with closed reduction and hollow screw internal fixation , while the control group were given closed reduction and hollow screw internal fixation merely .The length of stay , time of fracture healing , wound healing state , postoperative complications rate , Harris score and functional recovery of the two group were recorded and evaluated .Re-sults The average hospitalization time and fracture healing time in the PRP group were shorter than the control group .The A-grade recovery rate of the PRP group was higher than the control group , and the postoperative femoral head necrosis and nonunion rate of the PRP group were lower than that in the control group (P<0.05).The Harris score of PRP group were 87.35 and 90.82 respectively, and the excellent rate of hip function 6 months and 12 months after operation were 86%and 90%respectively .They were both higher than the control group with statistical significance (P<0.05).Conclusion PRP combined with closed reduction and hollow screw internal fixation can significant -ly shorten the healing time , reduce postoperative complications , improve hip function and postoperative quality of life in treating femoral neck fracture, and it is of high safety and efficacy.

Objective To establish a method for determination of total hardness in drinking water by using automatic potentiometric titrator.Methods Dynamic equivalence point titration(DET)and monotonic equivalence point titration(MET)mode was used to determine the high total hardness and low total hardness of drinking water samples respectively.Results Used DET mode to determine the high total hardness,the relative standard deviation(RSD)was 0.69%～1.72% and the recovery rate was 101.5%～102.2%.Used MET mode to determine the low total hardness,RSD was 3.49%～4.00% and the recovery rate was 95.8%- 103.6%.Conclusion This method is rapid,simple,accurate,precise and applicable to the determination of total hardness in drinking water in low and high levels.