Drug Delivery Systems ; Humans ; Neoplasms ; drug therapy ; etiology ; pathology ; Neoplastic Stem Cells ; pathology ; physiology ; Neovascularization, Pathologic

Animals ; Apoptosis ; Cell Cycle ; Cell Differentiation ; Cell Proliferation ; Connexin 43 ; metabolism ; Humans ; Neoplasm Invasiveness ; pathology ; physiopathology ; Neoplasms ; metabolism ; pathology ; physiopathology ; Neoplastic Stem Cells ; metabolism ; pathology ; physiology ; Neovascularization, Pathologic ; metabolism ; pathology ; physiopathology ; Receptors, CXCR4 ; metabolism ; Receptors, Formyl Peptide ; metabolism ; Vascular Endothelial Growth Factor A ; metabolism

Animals ; Cell Proliferation ; Disease Progression ; Endothelial Cells ; pathology ; Humans ; Indoleamine-Pyrrole 2,3,-Dioxygenase ; metabolism ; Mesenchymal Stromal Cells ; immunology ; metabolism ; pathology ; Neoplasms ; immunology ; pathology ; Neoplastic Stem Cells ; pathology ; Neovascularization, Pathologic ; pathology ; Stem Cells ; pathology ; T-Lymphocytes ; immunology ; pathology

Antigens, CD34 ; genetics ; metabolism ; Capillaries ; metabolism ; pathology ; Genetic Heterogeneity ; Microcirculation ; Neoplasms ; blood supply ; Neovascularization, Pathologic ; genetics ; metabolism ; pathology ; Phenotype ; Platelet Endothelial Cell Adhesion Molecule-1 ; genetics ; metabolism

Objective To analyze the clinicopathologic factors related to the prognosis of gallbladder carcinoma.Methods A retrospective analysis was conducted on 61 cases of advanced gallbladder carcinoma who were operated on from 2001 to 2006 in Nanjing First Hospital.Results According to the 6th UICC/AJCC TNM classification and staging of gallbladder carcinoma,of the 61 patients,8 patients were stage IB,22 were IIA,11 were IIB,14 were III and 6 were IV.Palliative cholecystectomy and/or bile duct drainage procedures were performed in 24 patients;removal of gallbladder plus regional lymph nodes and fatty tissue dissections were performed in 14 cases;radical resection was performed in 7 patients;removal of gallbladder and part of liver in combination with resection of bile duct(radical operatian) was performed in 13 patients;1 patient had extensive radical operation including pancreaticoduodenectomy.In 37 patients,18 had positive lymph node metastasis.Fifty-two patients were followed up,41(67.2%) patients were alive at 6 months after operation,26(42.6%)were alive at 12 months,10(16.4%) were alive at 24 months,and 7 cases were alive at 36 months,1 patient is still alive 5 years after operation;51 patients died within 2 years after operation.The symptoms of recurrence in 31 patients included progressing jaundice(21 cases),metastasis in liver(14 cases),and refractory ascites(12 cases).Five patients died of non-bile duct disease.Conclusions The prognosis of gallbladder cancer has a close relationship with the clinical stage and method of resection.

Objective To compare the results of different operative methods for old acromioclavicular dislocation. Methods Forty three patients with old acromioclavicular dislocation were treated with three different operative methods: 1)open reduction plus Kirschner wire fixation (9 cases); 2)open reduction plus reconstruction of acromioclavicular ligament (14 cases) and 3)open reduction plus modified Dewar s operation (20 cases). After surgery, the subjective symptoms of the arm, muscular contraction power, function of the shoulder joint and space of acromioclavicular joint in 43 cases were followed up for an average 4.8 years. Results The excellent results following three operative methods were seen in 33％ , 50％ and 74％ respectively. There was no significant difference between Kirschner wire fixation and reconstruction of acromioclavicular ligament (P > 0.05). Modified Dewar's operation was better compared with Kirschner wire fixation and reconstruction of acromioclavicular ligament (P< 0.05). Conclusion A satisfactory surgical procedure for treatment of old acromioclavicular dislocation should accomplish the following points: removal of scar tissue and intra articular cartilaginous fragments, reconstruction of joint stability and effective internal fixation until complete healing of the ligament structures.

To investigate the mechanism of Herba Epimedii alcoho l extract for imp otence, Giess reagent was used to observe the effect of the alcohol extract of H erba Epimedii in mice and its effect on release of NO fro m human umbilical vein endothelial cells in vitro was also studied. The results showed that alcoh ol extract of Herba Epimdii had no effect on the release of NO. But the serum co llected from the mice after the medication of alcohol extract of Herba Epimedii for 120min and 180min promoted the production of NO, and the reaction reached th e peak at 120 min. It is indicated that the promotion of NO release from endothe lial cells may be one of the mechanisms of Herba Epimedii for impotence.

To explore the antihepatotoxic mechanism of alcohol_extract of Xiao Chaihu Decoction (XCD),Giress reagent was used to observe the effect of the sero_alcohol_extract of XCD and alcohol_extract of XCD on the release of NO from mouse peritoneal macrophage in vitro.The results showed that alcohol_extract of XCD had no effect on the release of NO while the production of NO was improved in the cultured peritoneal macrophage after the medication of sero_alcohol_extract of XCD for 120min,180min and 240min.It is indicated that improving the release of NO from macrophage was one of the mechanisms of XCD in counteracting hepatic damage.

To investigate the mechanism of Herba Epimedii alcohol extract for impotence, Giess reagent was used to observe the effect of the alcohol extract of Herba Epimedii in mice and its effect on release of NO from human umbilical vein endothelial cells in vitro was also studied. The results showed that alcohol extract of Herba Epimdii had no effect on the release of NO. But the serum collected from the mice after the medication of alcohol extract of Herba Epimedii for 120min and 180min promoted the production of NO, and the reaction reached the peak at 120 min. It is indicated that the promotion of NO release from endothelial cells may be one of the mechanisms of Herba Epimedii for impotence.

Objective:This paper intends to provide reference for consummation of biosimilar registration poli-cies in China. Methods:This paper evaluates the performance of Japanese registration regulation policies on biosimi-lars by analyzing the procedure and reviewing the features of biosimilars. Results and Conclusions:According to the findings of the present investigation, the availability and affordability of biosimilars have been improved, and the bio-medicine industry has achieved great development. On the other hand, it's recommended that the guideline for the de-velopment and evaluation of biosimilars should be amended on the principle of appropriate simplification, which means to simplify the research and development procedure and exempt partial application dossier. Besides, the regis-tration procedures should be adopted continuously to ensure the stability of biosimilars' R&D and registration.