OBJECTIVETo analyze the meiotic segregation results of male reciprocal chromosome translocation by fluorescence in situ hybridization (FISH).

METHODSMulti-color FISH using 3 combined probes located in any 3 chromosome segments on both sides of two breakpoints was performed on the de-condensed sperm head to analyze the sperm chromosomal contents and segregation patterns.

RESULTSFour male reciprocal translocation carriers were included in the study, with the karyotypes of 46, XY, t(2;18) (p16; q23); 46, XY, t(4;6) (q34;q21); 46, XY, t(8;13) (q23;q21) and 46, XY, t(4;5) (4q31;5q13), respectively. The results showed that 4 carriers had different proportions of various segregated spermatozoa. The spermatozoa of alternate, adjacent-1, adjacent-2, 3:1, non-disjunction in meiosis II, and 4:0 or diploidy accounted for 27.1%-49.4%, 26.9%-37.6%, 2.7%-15.7%, 8.6%-32.7%, 0.2%-1.9%, and 0.1%-0.4%, respectively.

CONCLUSIONFor each-reciprocal translocation carrier seems to have a particular meiotic segregation results, FISH analysis on sperm head should be done for each carrier in order to provide an accurate genetic counseling.

Chromosome Breakage ; Heterozygote ; Humans ; In Situ Hybridization, Fluorescence ; Karyotyping ; Male ; Meiosis ; genetics ; Spermatozoa ; metabolism ; Translocation, Genetic ; genetics

Eye Diseases ; prevention & control ; Home Care Services ; Humans ; Schools ; Single-Blind Method ; Students ; Urban Population

Objective To study the influence of nasal irrigations on the postoperative curative effect of patients with chronic sinusitis.Methods Total of 357 patients who suffered from chronic sinusitis received transnasal endoscopic surgery from 2008 to 2010 with 184 patients selecting nasal irrigations for the following treatments.Patients were followed up at two weeks,six weeks and four months after the surgeries via telephone.The symptoms of nasal obstruction,nasal discharge and headache were recorded and SNOT-20 was used to measure the quality of life.Results Symptoms of patients were improved,but improvement in nasal irrigations group was better than that in non-nasal irrigations group.Four months later,the average symptom score of nasal obstruction in nasal irrigations group was [0(0,1 )] which was significantly lower than [ 0( 0,1 )] in non-nasal irrigations group ( Z =- 3.176,P ＜ 0.05).The average symptom score of nasal discharge in nasal irrigations group was [0(0,2)] which was significantly lower than [0(0,2)] in non-nasal irrigations group (Z =- 4.403,P ＜ 0.05 ).The average score of headache was [ 0 ( 0,1 ) ] in nasal irrigations group and [ 0 (0,2) ] in non-nasal irrigations group,and the difference was statistically significant ( Z =- 4.539,P ＜ 0.05 ).Conclusions Nasal irrigations can improve symptoms of patients with chronic sinusitis and reduce the rectrrence.