1.Effect of Rapamycin on Proliferation and Cell Cycle of Human Bladder Carcinoma Cell Line 5637
Guangyi SHAN ; Cheng FU ; Ang CHEN ; Bin HU ; Huan BI
Journal of China Medical University 2015;(3):230-233
Objective To evaluate the effect of rapamycin on proliferation and cell cycle of human bladder cancer cell line 5637. Methods Total?ly 5637 cells were maintained in RPMI 1640 containing 10%fetal bovine serum,100 U/mL penicillin and 100μg/mL streptomycin for adherent cul?ture. Cells were grown at 37℃in a 5%CO2 incubator. The 5637 cells were treated with various concentrations of rapamycin solution(50 nmol/L, 100 nmol/L,200 nmol/L,and 400 nmol/L). The control group was daily treated with single RPMI 1640 without medication. The growth repression rate for 5637 cells was evaluated by MTT. Flow cytometry was used to investigate the effect of rapamycin of different concentrations on cell cycle of 5637 cells. Results Compared to the control group,rapamycin can inhibit the proliferation of 5637 cells in a concentration and time dependent manner. Rapamycin inhibited 5637 cells at G0/G1 thus inhibiting cell proliferation but not inducing apoptosis. Conclusion Rapamycin inhibited growth of 5637 bladder carcinoma cells and arrested cell cycle at G0/G1,indicating that mammalian target of rapamycin may play an important role in proliferation of 5637 cells and act as a new tumor therapeutic target in patients with bladder cancer.
2.Risk factors of nosocomial infection of patients with spinal cord injury
Ying HUAN ; Zhao-kong LIU ; Bin HUAN ; Guiling CHENG ; Yanping BI ; Min LIU
Chinese Journal of Rehabilitation Theory and Practice 2004;10(2):90-91
ObjectiveTo analyze the risk factors of nosocomial infection of patients with spinal cord injury.MethodsRetrospective surveys of nosocomial infection including risk factors, pathogen analysis and drug sensitivity tests were carried out for 88 cases with spinal cord injury in our hospital from 1996 to 2002.ResultsIt showed that the rate of nosocomial infection was 90.91% with 46.59% of urinary tract infection and 32.95% of respiratory tract infection. Nosocomial infection was closely related with such factors as the disease of spinal cord, the use of antibiotics, invasive operation, age and disturbance of disease.ConclusionReducing risk factors and using antibiotics reasonably are effective measures to reduce nosocomial infection of patients with spinal cord injury.
3.A comparative study of the bracket bond failure between adults and adolescents receiving fixed orthodontic therapy.
Li-wei XIAO ; Yang-xi CHEN ; Ding BAI ; Jian ZHANG ; Bi-huan CHENG
Chinese Journal of Stomatology 2003;38(3):233-235
OBJECTIVETo evaluate the bracket bond failure and its causes between adult and adolescent patients during fixed orthodontic therapy.
METHODSBracket bond failure data of 30 adults and 30 adolescents, receiving fixed orthodontic therapy, have been collected within the first 12 visits, respectively. The compliance has been analyzed with survival analyse between the two groups.
RESULTThe general bracket bond failure rate in the adult group is lower than that of the adolescent group and the difference is significant (p < 0.05). In the adolescent group, the failure rate for mandibular anterior teeth is highest and different from that of the adult group (p < 0.05). The failure rate resulted from biting hard food is ranked No. 1.
CONCLUSIONSThe compliance of the adults receiving fixed orthodontic therapy is better than that of the adolescents. The investigation of bracket bond failure causes is beneficial in helping orthodontists improve orthodontic practice and raise clinical efficiency. The survival analysis is effective in evaluating the bond failure.
Adolescent ; Adult ; Equipment Failure ; Female ; Humans ; Male ; Orthodontic Brackets ; Orthodontics, Corrective ; Survival Analysis
4.Resection of pancreatic capsule and anterior layer of transverse mesocolon in radical gastrectomy and its clinical significance.
Jian-wei BI ; Cheng-hui DU ; Guo WEI ; Kang-kang ZHI ; Ting HAN ; Guang-huan XU ; Ji-de HUA
Chinese Journal of Gastrointestinal Surgery 2009;12(5):467-470
OBJECTIVETo investigate the clinical significance of resection of the pancreatic capsule and anterior layer of transverse mesocolon in radical gastrectomy.
METHODSBetween January 2007 and July 2008, a total of 213 gastric cancer patients enrolled in the study. These patients were randomly assigned into two groups: 105 in group R and 108 in group N. Only in group R were the pancreatic capsule and anterior layer of transverse mesocolon resected during radical gastrectomy. The pancreatic capsule and anterior layer of transverse mesocolon were histologically analyzed for metastasis. The data including blood loss during operations, number of dissected lymph nodes and postoperative complications were analyzed in both groups.
RESULTSThere were no significant differences between the two groups in blood loss during operation and postoperative complications, but the differences in operation time and number of dissected lymph nodes between the two groups were significant. Metastases to the pancreatic capsule and/or anterior layer of transverse mesocolon were diagnosed in nine (8.6%) patients of group R. The metastases to the pancreatic capsule and/or anterior layer of transverse mesocolon were found to be associated with tumor invasion depth, anterior or posterior gastric wall, clinical staging and perigastric lymph node metastasis extent (P<0.05), but not with age, gender, tumor location, size, Borrmann type and pathological classification (P>0.05).
CONCLUSIONSResection of pancreatic capsule and anterior layer of transverse mesocolon in group R does not increase postoperative complications in comparison with group N. The resection is beneficial to the patients with advanced gastric cancer staging relatively late because of potential metastasis to pancreatic capsule and anterior layer of transverse mesocolon.
Adult ; Aged ; Aged, 80 and over ; Colon, Transverse ; Female ; Gastrectomy ; methods ; Humans ; Male ; Mesocolon ; surgery ; Middle Aged ; Pancreas ; surgery ; Stomach Neoplasms ; surgery
5.Dynamic changes of cyclophosphamide-induced liver injury in mice
Can HUANG ; Fa-jing HE ; Xiao YANG ; Li-huan GUAN ; Si-min ZHANG ; Yan-ying ZHOU ; Shi-cheng FAN ; Xin-peng YAO ; Min HUANG ; Hui-chang BI
Acta Pharmaceutica Sinica 2019;54(6):1062-1068
Cyclophosphamide (CPA) is one of the most commonly used alkylating agents in the treatment of malignant cancer. CPA is metabolized by cytochrome P450 enzymes into 4-hydroxycyclophosphamide