1.Early enteral nutrition by transcutaneous catheter jejunostomy in gastric cancer patients after total gastrectomy
Zhaoxu ZHENG ; Qiang FENG ; Qian LIU ; Jianjun BI ; Weiqi RONG ; Xinghua YUAN
Chinese Journal of General Surgery 2012;27(6):495-498
Objective To investigate the effects on the condition of nutrition and immunologic function of gastric cancer treated with the insertion of jejunal nutrient canal after total gastrectomy.Methods In this study 113 gastric cancer patients were randomly divided into enteral nutrition group (the group of the fine-needle/catheter jejunostomy during operation,FCJ group) and parenteral nutrition group (PN group) after total gastrectomy.Evacuating time and postoperative complications were observed and relative laboratory parameters were measured prior to surgery (preoperative) and on days 3 and 7 postoperatively.Results The evacuating time in enteral nutrition group was shorter than that in parenteral nutrition group significantly[(4.1±2.2) d vs.(5.1 ±2.0) d,t =2.156,P =0.037];Serum level of prealbumin[( 18 ± 7 ) mg/dl vs.( 14 ± 7 ) mg/dl,t =2.370,P =0.022]and transferring[(205 ±45 ) mg/dl vs.( 186 ± 39 ) mg/dl,t =3.665,P =0.001]in enteral nutrition group on postoperative day 7 was higher than that in parenteral nutrition group;Serum IgA[( 2.3 ± 1.0 ) g/L vs.( 1.9 + 0.7 ) g/L,t =2.178,P=0.034],lgM[(1.4 ±0.4) g/L vs.(1.0 ±0.4) g/L,t=2.124,P=0.039]and IgG[(9.5 ±1.9) g/L vs.(9.0 ± 2.3 ) g/L,t =2.189,P =0.033]were higher in enteral nutrition group than that in parenteral nutrition group;The incidence of postoperative alimentary dysfunction in enteral nutrition group was lower than that in parenteral nutrition group( 3% vs.13%,x2 =3.962,P =0.048).Conclusions It is safe and convenient to use early postoperative enteral nutrition support by fine-needle/catheter jejunostomy (FCJ) in gastric cancer patients immediately after total gastrectomy.
2.Right upper abdominal wall malignant fibrous xanthomas complicated with ileocecal adenocarcinoma and uterine leiomyoma: a case report.
Yu-feng YUAN ; Zi-su LIU ; Qun QIAN ; Bi-cheng WANG ; Yue-ming HE ; Yun-hua WU ; Ke-yan ZHENG ; Cong-qian JIANG ; Zhong-li AI
Chinese Journal of Oncology 2005;27(12):716-716
Abdominal Wall
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Adenocarcinoma
;
diagnosis
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Colonic Neoplasms
;
diagnosis
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Female
;
Histiocytoma, Malignant Fibrous
;
diagnosis
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Humans
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Ileal Neoplasms
;
diagnosis
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Ileocecal Valve
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Leiomyoma
;
diagnosis
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Middle Aged
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Neoplasms, Multiple Primary
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diagnosis
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Soft Tissue Neoplasms
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diagnosis
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Uterine Neoplasms
;
diagnosis
3.An efficient tool for the construction of multiple-cistronic vectors: FMDV 2A.
Bi-Sheng LIU ; Xin-Yuan LIU ; Cheng QIAN
Chinese Journal of Biotechnology 2007;23(5):765-769
Recently, cancer therapy with mutiple genes has been attached with great attention. However, at present there is no efficient tool to construct multiple-cistrons. The large sizes and the imbalance in expression of most traditional tools, such as ribosome entry sites (IRESes),greatly block their wide employment in the construction of multiple cistronic gene therapy vectors. The self-cleaving peptide 2A from foot-and-mouth disease virus (FMDV) has a very small size, and more importantly, high cleavage activity in artifical bicistron, which bring new hope for mutiple genes therapy stategy. In this article, the characteristics and cleavage activities of FMDV 2A will be elucidated,and we further outline its applications in cancer gene therapy.
Amino Acid Sequence
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Artificial Gene Fusion
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DNA, Recombinant
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Foot-and-Mouth Disease Virus
;
genetics
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Genetic Therapy
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methods
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Genetic Vectors
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genetics
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Molecular Sequence Data
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Neoplasms
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therapy
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Promoter Regions, Genetic
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RNA Splicing
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Transcription Factors
4.Analysis on causes of death of childhood leukemia from 1981 to 2000 in the city of Tianjin.
Ke-Xin CHEN ; Guang-Lin WU ; Min HE ; Shu-Fen DONG ; Ji-Fang WANG ; Bi-Yuan QIAN
Chinese Journal of Pediatrics 2004;42(8):617-620
OBJECTIVELeukemia is a major cause of death of children in China, which accounts for 50 % of all cancers of children. Data from Tianjin Cancer Hospital was analyzed for mortality of leukemia in children under 20 years from 1981 to 2000 in the city of Tianjin.
METHODSAll physicians and medical staff of the hospitals and clinics in the registry area were responsible for filling out the report forms for every new case diagnosed as malignant tumors. Death certificates for malignant tumors have been registered at the local police station and the residential files were checked. All cancer cases with insufficient information were traced to his/her family and relevant persons worked in the clinic. Tianjin Cancer Registry Center periodically conducted an active re-checking program to review all patient records on cancers that was not registered in this period. Tumors diagnosed in this study were coded according to the International Classification of Diseases for Oncology (ICD-O). Mortality rates were calculated by age, sex and date of death.
RESULTSThe types of acute lymphoid leukemia, acute myeloid leukemia and chronic myeloid leukemia were the most common types of childhood leukemia in Tianjin, comprised 69.3%, 20.9 % and 8.0%, respectively. The mortality for childhood leukemia decreased slowly during the period of 1981 to 2000 in Tianjin. Mortality and morbidity ratios were 0.51.
CONCLUSIONCombined with characteristics of individual forms of childhood leukemia mortality, further epidemiological research is needed to prevent childhood leukemia.
Adolescent ; Cause of Death ; Child ; Child, Preschool ; China ; epidemiology ; Humans ; Infant ; Leukemia ; mortality ; Young Adult
5.Improved anti-tumor efficacy and pharmacokinetics of bufalin via PEGylated liposomes
YUAN JIA-NI ; ZHOU XUAN-XUAN ; CAO WEI ; BI LIN-LIN ; ZHANG YI-FANG ; YANG QIAN ; WANG SI-WANG
Chinese Journal of Pharmacology and Toxicology 2017;31(10):978-979
OBJECTIVE To determine the characterization, anti-tumor efficacy and pharmacokinetics of bufalin- loaded PEGylated liposomes compared with bufalin entity. METHODS Bufalin- loaded PEGylated liposomes and bufalin- loaded liposomes were prepared reproducibly with homogeneous particle size by the combination of thin film evaporation method and high pressure homogenization method. The particle size and zeta potential of the liposomes were determined by dynamic light scattering technique. The direct imaging of morphology of liposomes was charactered by transmission electron microscope. The content of bufalin in liposomes was analysed by HPLC method. The entrapment efficiency and the particle size was applied to assess the stability profile, after storage at 4℃ on day 0, 7, 15, 30 and 90. The in-vitro release behaviours of bufalin from liposomes were conducted using dialysis bag technique at 37℃. In-vitro cytotoxicity studies were carried out using MTT〔3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide〕assay on several kinds of tumor cell lines including SW620, PC-3, MDA-MB-231, A549, U251, U87 and HepG2. In-vivo pharmacokinetic study of bufalin liposomes was evaluated by HPLC method. RESULTS Their mean particle sizes were 127.6 nm and 155.0 nm, mean zeta potentials were 2.24 mV and - 18.5 mV, entrapment efficiencies were 76.31% and 78.40% , respectively. In- vitro release profile revealed that the release of bufalin in bufalin- loaded PEGylated liposomes was slower than that of bufalin-loaded liposomes. The cytotoxicity of blank liposomes has been found within acceptable range, whereas bufalin-loaded PEGylated liposomes showed enhanced cytotoxicity to U251 cells compared with bufalin entity. In-vivo pharmacokinetics indicated that bufalin-loaded PEGylated liposomes could extend eliminate half-life time of bufalin in plasma in rats. CONCLUSION The results suggested that bufalin-loaded PEGylated liposomes improved the solubility and increased the drug concentration in plasma.
6.Analysis of variation trends of thyroid cancer treated in Tianjin Cancer Hospital form 1954 to 2009.
Song-Feng WEI ; Ming GAO ; Bi-Yun QIAN ; Yi-Gong LI ; Yan ZHANG ; Wen-Yuan CHEN ; Xiang-Qian ZHENG ; Xiao-Long LI ; Yang YU ; Yong CUI
Chinese Journal of Oncology 2011;33(8):613-615
OBJECTIVETo investigate and analyze the variation trends in the pathological composition of thyroid cancer patients treated in Tianjin Cancer Hospital from 1954 to 2009.
METHODSTo retrospectively analyze the incidence and clinical features of different pathological types of thyroid cancers in 4342 patients between different time periods from 1954 to 2009.
RESULTSIn the four main pathological types of thyroid cancers, the component ratio of papillary thyroid cancer in every period was 68.1%, 78.3%, 81.3%, 82.1%, 85.8%, respectively, while the morbidity of patients with papillary thyroid carcinoma concurrent with Hashimoto's thyroiditis was increased, so was the proportion of tumors in diameter < or = 2 cm. The proportion of follicular thyroid carcinoma and anaplastic thyroid carcinoma was decreasing accordingly; however, the proportion of medullary thyroid carcinoma did not change significantly.
CONCLUSIONSThe pathological classification of the thyroid carcinoma patients has significant changes in the 4342 cases treated in our Hospital from 1954 to 2009. The proportion of papillary carcinoma is increased, while that of follicular carcinoma and anaplastic carcinoma is decreased. The reasons might attribute to the improved level of consultations and iodized diet or other factors.
Adenocarcinoma, Follicular ; epidemiology ; pathology ; Carcinoma ; epidemiology ; pathology ; Carcinoma, Medullary ; epidemiology ; pathology ; Carcinoma, Papillary ; epidemiology ; pathology ; China ; epidemiology ; Female ; Hashimoto Disease ; complications ; epidemiology ; pathology ; Humans ; Incidence ; Male ; Retrospective Studies ; Thyroid Neoplasms ; complications ; epidemiology ; pathology ; Tumor Burden
7.Baculovirus expression of two human recombinant neutralizing IgG monoclonal antibodies to hepatitis A virus.
Jing-yuan CAO ; Mi-fang LIANG ; Qing-ling MENG ; Xiao-fang WANG ; Yong-gang XU ; Ke-qian GUO ; Mei-yun ZHAN ; Sheng-li BI ; De-xin LI
Chinese Journal of Experimental and Clinical Virology 2004;18(1):20-23
OBJECTIVETo develop human recombinant neutralizing IgG monoclonal antibodies to hepatitis A virus (HAV) by baculovirus expression system.
METHODSThe heavy and light chain genes of two human-derived neutralizing Fab antibodies to HAV were cloned into baculovirus expression vector Pac-kappa-Fc and Pac-L-Fc, and further expressed in insect cells as IgG antibodies. The IgG products were purified and well characterized.
RESULTSThe baculovirus expressed McAb HAFc16 fully retained the specificity of binding to hepatitis A virus and the competition with mouse anti-hepatitis A virus McAb using ELISA. The viral neutralization assay in vitro demonstrated the retention of antibody function after expression of the human antibody in insect cells. The other expressed antibody HAFc78 also has the neutralizing activity but it is directed against different epitopes of HAV when compared with HAFc16.
CONCLUSIONThe recombinant baculovirus/insect cells expressed human neutralizing IgG antibodies to hepatitis A virus retained all biological functions specific for hepatitis A virus. The results provided the possibility of using these antibodies to rapidly protect high risk or early exposure populations from hepatitis A virus infection.
Antibodies, Monoclonal ; biosynthesis ; immunology ; Baculoviridae ; genetics ; Hepatitis A virus ; immunology ; Hepatitis Antibodies ; biosynthesis ; immunology ; Humans ; Immunoglobulin Fab Fragments ; biosynthesis ; immunology ; Immunoglobulin G ; biosynthesis ; immunology ; Immunoglobulin Heavy Chains ; genetics ; Immunoglobulin Light Chains ; genetics ; Recombinant Proteins ; biosynthesis ; immunology
8.Analyses of Streptomyces coelicolor inner membrane proteome by multidimentional protein identification technology.
Xuan-Ming SHI ; Yuan-Ming LUO ; Gui-Feng ZHANG ; Zhi-Guo SU ; Yu-Bi HUANG ; Ke-Qian YANG
Chinese Journal of Biotechnology 2005;21(5):814-819
Streptomyces coelicolor is the model species among streptomycetes. Until now, proteomic analyses of S. coelicolor have been conducted using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry method, few integral membrane proteins were identified due to the hydrophobic and low-abundance nature of these proteins. In this work, 154 possible inner membrane proteins from S. coelicolor were identified using high pH-proteinase K sample preparation method and multidimensional protein identification technology, among them 44 are integral membrane proteins containing at least one transmembrane domain, most peptides and their corresponding proteins were identified experimentally for the first time.
Bacterial Proteins
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analysis
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Cell Membrane
;
chemistry
;
Genome, Bacterial
;
genetics
;
Mass Spectrometry
;
methods
;
Membrane Proteins
;
analysis
;
Proteome
;
analysis
;
genetics
;
Streptomyces coelicolor
;
chemistry
;
genetics
9.Genotyping of hepatitis A virus prevalent strains in Xinjiang Hetian of China in 2006.
Ayiguli YIERHALI ; Jing-yuan CAO ; Aideer AILI ; Qian WEN ; Shi-ping YANG ; Re-xi KU ; Qing-ling MENG ; Xin-lan LI ; Sheng-li BI
Chinese Journal of Experimental and Clinical Virology 2009;23(5):358-360
OBJECTIVETo analysis the genotypes of wild type hepatitis A virus circulated in Xinjiang Hetian of China in 2006.
METHODSThe Vp1-2A region of HAV genome was amplified and sequenced from serum samples collected in Xinjiang Hetian of China in 2006, and subjected to phylogenetic analysis by Neighbor Joining (NJ) method.
RESULTSThe nucleotide sequence differences in the VP1-2A region among Xinjiang Hetian HAV strains ranged from 0%-3.9%, all belonged to sub-genotype 1A. Genetically similar strains were identified among Xinjiang Hetian 2006 and Xinjiang Yili 2005 of China isolates. Only 0-2 amino acid differences were found among the Xinjiang Hetian HAV isolates in the VP1-2A region.
CONCLUSIONThere were different HAV strains existing in the investigated areas, these strains may have different transmission pathways for the spread of the disease. The results indicate the usefulness of molecular epidemiological methods in studying changes in the circulating HAV strains and in tracing transmission routes, and also for effectively control measures to prevent the spread of the disease.
China ; epidemiology ; Genotype ; Hepatitis A ; epidemiology ; immunology ; virology ; Hepatitis A Antibodies ; blood ; Hepatitis A Virus, Human ; classification ; genetics ; immunology ; isolation & purification ; Humans ; Molecular Sequence Data ; Phylogeny ; RNA, Viral ; blood ; genetics ; Viral Structural Proteins ; genetics
10.Prokaryotic soluble expression of protein D of Haemophilus influenzae type b.
Meng-Meng YIN ; Qiu-Dong SU ; Min-Zhuo GYO ; Yi-Na CUN ; Yuan-Qian PU ; Zhi-Yuan JIA ; Jing-Ran YANG ; Yang TANG ; Guo-Yang LIAO ; Yao YI ; Sheng-Li BI ; Wei-Dong LI
Chinese Journal of Experimental and Clinical Virology 2013;27(2):89-91
OBJECTIVETo express the recombinant D protein in prokaryotic expression system solubly and make preparation for producing D-carrier conjugate vaccine next step.
METHODSThe hpd gene fragment removed of signal peptide from genomic DNA of Hib CMCC was inserted into pET43. 1a. The recombinant plasmid was transformed to competent E. coli BL21 (DE3) for expression under induction of IPTG. The expressed recombination protein was precipitated with ammonium sulfate, purified by DEAE anion exchange column chromatography and identified for reactogenicity by Western Blot.
RESULTSThe expressed recombination protein, in a soluble form, constained about 50% of total somatic protein and showed specific reaction with the HIB antisera after preliminary purification.
CONCLUSIONThe D protein recombined expression plasmid was constructed successfully and expressed D protein in prokaryotic cells in a solube form.
Bacterial Proteins ; genetics ; Blotting, Western ; Carrier Proteins ; genetics ; Escherichia coli ; genetics ; Haemophilus influenzae type b ; genetics ; Immunoglobulin D ; genetics ; Lipoproteins ; genetics ; Plasmids ; Recombinant Proteins ; biosynthesis ; Solubility