1.Effect of xingding injection on platelet coagulation and fibrinolysis activity in patients with advanced stage chronic pulmonary heart disease.
Bi-yun SUN ; Jing-lin YANG ; Xue-ying CHEN
Chinese Journal of Integrated Traditional and Western Medicine 2004;24(2):154-155
Aged
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Aged, 80 and over
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Drugs, Chinese Herbal
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therapeutic use
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Female
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Fibrin Fibrinogen Degradation Products
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metabolism
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Fibrinolysis
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drug effects
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Glycoproteins
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blood
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Humans
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Injections
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Male
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Middle Aged
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Phytotherapy
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Plasminogen Activator Inhibitor 1
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blood
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Platelet Aggregation
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drug effects
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Pulmonary Heart Disease
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blood
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drug therapy
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Tissue Plasminogen Activator
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blood
2.Morphological changes of apoptotic brain neuroglioma cells induced with methyl-mercuric chloride and significances
Ying LIU ; Xuan CHEN ; Xiaoying BI ; Zhichao LI ; Changji YUAN
Journal of Jilin University(Medicine Edition) 2006;0(04):-
Objective To study the anti-neuroglioma effect of methyl-mercuric chloride(MMC) by observing the morphological changes of apoptotic neuroglioma cells induced with MMC in rats with brain neuroglioma.Methods The rat models of neuroglioma were established,and divided into two groups.The rats in experimental group were lavaged with MMC 1 week after injected with C6 glioma cells,10 mg?kg-1every day,the rats in control group were treated with sodium chloride at the same dose.24 d after inoculation all rats were sacrificed except natural death,the brain tissues were obtained,and the pathohistological changes were observed under light microscope and transmission electron microscope.Results The macropathological result showed that the tumor volume in experimental group was smaller than that in control group.Under light microscope,in experimental group the growth density of C6 ghioma cells was lower than that in control group,and the apoptotic cells with smaller volume and karyopyknosis were found.The result of transmission electron microscope showed that in experimental group,the glioma cells had some changes such as karyopyknosis,chromoplasm margination,nuclear fragmentation and vacuolar degeneration and so on.Conclusion MMC has inhibitory effect on the proliferation of C6 glioma in rats in vivo,its mechanism may be related to inducing the apoptosis of neuroglioma cells.
3.Primary hepatocyte culture and its application in drug metabolism and toxicology research and evaluation
Ying DENG ; Jie CHEN ; Huichang BI ; Min HUANG
Chinese Pharmacological Bulletin 2003;0(08):-
For more than 40 years primary hepatocytes culture technique has been utilized extensively for assessing effects of drug on metabolizing enzymes (especially cytochromes P450), drug metabolism, drug-drug interactions, and the mechanisms of cytotoxicity and genotoxicity. Human derived primary hepatocytes reserve the metabolism function and enzyme activity of liver. Therefore, this technique has been widely used as a reliable and efficient tool in the drug and xenobiotics evaluation and screen in vitro. This review focuses on primary culture technique of hepatocytes and its application in drug metabolism and toxicology research and evaluation.
4.High Expression of Long Non-coding RNA HOTTIP Indicates Poor Prognosis in Cancers: Evidence from Six Studies
Ying CHEN ; Guoqing WEI ; Hongwei XIA ; Feng BI ; Ming LIU
Progress in Modern Biomedicine 2017;17(22):4376-4381
Objective:The significant role of long non-coding RNAs (lncRNAs) in early diagnosis and predicting prognosis has been recognized in various cancers recently.However,the prognostic value of HOXA transcript at the distal tip (HOTTIP),a vital lncRNA in tumorigenesis,remains unclear.In this study,we evaluated its prognostic value by analyzing the correlation of HOTTIP expression with overall survival (OS),lymph node metastasis (LNM) and distant metastasis (DM) in different cancer types by meta-analysis.Methods:We performed a systematic search in PUBMED,MEDLINE,Web of Science and Cochrane Library update to November of 2016.A total of 604 patients from 6 studies were included in final analysis and went through a quantitative meta-analysis by Review manager 5.3.Results:We demonstrated that high expression of HOTTIP had a significant correlation with poor OS (hazard ratio [HR] =2.37,95% confidence interval [CI] =1.81-3.10,p<0.001),high LNM rate (odds ratio [OR]=2.29,95%CI=1.54-3.40,p<0.001) as well as more DM occurrence (OR=3.30,95%CI=1.78-6.12,p<0.001).Conclusion:Our results indicated that long non-coding RNA HOTTIP may serve as a potential prognostic biomarker in cancer progression.
5.Primary MALT lymphoma of orbit:a clinicopathological study of 77 cases.
Ying-wen BI ; Rong-jia CHEN ; Ying-yong HOU ; Xia-ping LI
Chinese Journal of Pathology 2007;36(6):414-415
Adult
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Aged
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Aged, 80 and over
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Conjunctival Neoplasms
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diagnostic imaging
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pathology
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radiotherapy
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surgery
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Female
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Follow-Up Studies
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Humans
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Lymphoma, B-Cell, Marginal Zone
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diagnostic imaging
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pathology
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radiotherapy
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surgery
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Male
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Middle Aged
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Orbital Neoplasms
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diagnostic imaging
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pathology
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radiotherapy
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surgery
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Prognosis
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Retrospective Studies
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Tomography, X-Ray Computed
6.Assessment of myocardial perfusion and systolic function in patients with coronary artery disease after coronary artery bypass surgery by myocardial contrast echocardiography and two-dimensional strain echocardiography
Rong LIU ; Youbin DENG ; Yani LIU ; Xiaojun BI ; Li XIONG ; Ying ZHU ; Liuping CHEN
Chinese Journal of Ultrasonography 2009;18(8):645-648
two-dimensional strain echocardiography can observe dynamic change of regional systolic function,combined with the two methods can more accurately assess the treatment results of coronary artery bypass surgery.
7.Analysis of monosaccharide compositions in polysaccharides from exopleura of Ginkgo biloba.
Lei-Lei MAO ; Ying CHEN ; Bi-Yuan HU ; Ai-Hua XU
China Journal of Chinese Materia Medica 2014;39(2):262-266
A high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5 -pyrazolone (PMP) has been established for determination of 6 kinds of monosaccharides simultaneously. A special Agilent HC-C18 column (4. 6 mm x 250 mm, 5 microm), optimized for the separation of PMP derivatives, was used at ambient temperature of 40 degrees C. The PMP derivatives elution was performed with a mixture of 0.1 mol x L(-1) phosphate buffer (pH 6. 8) and acetonitrile in a ratio of 84: 16 at a flow rate of 1 mL x min(-1), and UV absorbance of the effluent was monitored at 245 nm. The results showed that the polysaccharides from exopleura of Ginkgo biloba were acidic heteropolysaccharides mainly containing mannose, rhamnose, D-galacturonic acid, glucose, galactose, arabinose, with the molar ratio of 0.032: 0.14: 0.296: 0.403:0.106: 0.046.
Ginkgo biloba
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chemistry
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Hydrolysis
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Monosaccharides
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analysis
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Plant Components, Aerial
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chemistry
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Polysaccharides
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chemistry
8.MRI diagnosis of single lesion in the corpus callosum department
Guoli BI ; Xiarong GONG ; Kunhua WU ; Ying ZHAO ; Maoping RUI ; Lixiang REN ; Yuhui CHEN
Journal of Practical Radiology 2016;(2):185-187
Objective To evaluate MRI diagnostic value for single lesion characteristics in the splenium of corpus callosum.Methods MRI features,clinical data,and parts of follow-up results of 9 cases with single lesion in the splenium of corpus callosum were analyzed retrospectively.Results (1)Clinical manifestations:headache and dizziness occurred in 4 cases,syncope in 3 cases,fever in 2 cases, physical activity barriers in 2 cases.(2)Clinical diagnosis:hypoglycemic encephalopathy were rescaned one month later in 3 cases, in which the previous lesion completely disappeared.Clinical experience of encephalitis were improved after treatment in 2 cases. Cerebral infarction,epilepsy,brain injury and degeneration were diagnosed respectively in each one case,in which lesion still existed after treatmented.(3)Image findings:despite the different clinical manifestations,image features of all cases were quite similar. Round or foliated like lesions of slightly long T1 and long T2 signals in the splenium of corpus callosum were presented in all cases. High signals on diffusion weighted imaging and low signals on the ADC were showed with same lesions,andno obvious enhancement after contract media injected was seen.Conclusion Single lesions in the splenium of corpus callosum are showed in many diseases. The image features of hypoglycemic encephalopathy or encephalitis have certain characteristics (single lesion is reversible).Accurate diagnosis need to combine with clinical data and medical history.
9.Mathematical Cognitive Ability Predicted by Amplitude of Low Frequency Fluctuate in Patients with Brain Dam-age
Ying ZHAO ; Qiang WANG ; Rong SUN ; Xiaoxia DU ; Luping SONG ; Lingjuan CHEN ; Yanchao BI ; Zaizhu HAN
Chinese Journal of Rehabilitation Theory and Practice 2015;21(6):670-676
Objective To find biological markers to predict the mathematical cognitive ability in order to set patients free from the pain and time-consuming behavioral tests. Methods 86 patients with stroke or brain traumatic injuries were recruited and acquired T1 and rest-ing-state functional MRI imaging data. And a mathematical task (7 calculation items, 2 counting items) and a word-reading task (140 items) was also finished. The partial correlative analysis was made between the score of mathematical task and the amplitude of low frequency fluc-tuation of each voxel of the whole brain with the word-reading performance as controlling task, and AlphaSim correction method was used with corrected P<0.05 (single voxel level:P<0.05;cluster size:>110 voxels). Results There were 5 cerebral regions whose amplitude of low frequency fluctuation significantly correlated with mathematical performance:left inferior parietal lobule (161 voxels, rpeak=0.34), left precu-neus/superior parietal lobule (141 voxels, rpeak=0.31), left middle temporal gyrus (359 voxels, rpeak=0.34), left middle frontal gyrus (491 vox-els, rpeak=0.36), and right middle frontal gyrus (156 voxels, rpeak=0.32). Conclusion The amplitude of low frequency fluctuation of left inferior parietal lobule, precuneus/superior parietal lobule, middle temporal gyrus, middle frontal gyrus, and right middle frontal gyrus could be used as predictors of mathematical cognitive ability for brain-damaged patients.
10.Surgical revision of lumbar vertebrae using transforaminal endoscopic spine system.
Hui YAO ; Yi-chun XU ; Bi-ying CHEN ; Gang HOU ; Hui-qing ZHAO
China Journal of Orthopaedics and Traumatology 2015;28(8):712-716
OBJECTIVETo explore the clinical effects of transforaminal endoscopic spine system in surgical revision of lumbar vertebrae.
METHODSFrom January 2012 to October 2013,14 patients who needed reoperations of lumbar vertebrae were treated using transforaminal endoscopic spine system (TESSYS). There were 8 males and 6 males, aged from 27 to 84 years old with an average of (50.4 ± 18.9) years. Visual analogue scale (VAS) and Japanese Orthopaedic Association Scores (JOA) were compared before and after surgical revision. Macnab standard was used to assess the clinical effect.
RESULTSAll the patients were followed up from 6 to 27 months with the mean of 18 months. Preoperative VAS score was 6.79 ± 1.31, and in a week,3 months and 6 months after operation were 2.50 ± 1.29, 2.21 ± 1.53, 1.64 ± 1.08, respectively, which were all much lower (P < 0.01) than preoperative score. Preoperative JOA score was 12.43 ± 1.95, and the above corresponding postoperative JOA scores were 21.50 ± 3.78, 21.93 ± 4.55, 23.36 ± 4.33, respectively, which were all much higher than preoperative score (P < 0.01). According to the modified Macnab criteria, 5 patients got an excellent results, 7 good, 1 fair and 1 poor. The nerve root injury of L5 occurred in 1 case during paracentesis and no other complications were found.
CONCLUSIONSelecting the appropriate indications using TESSYS in surgical revision of lumbar vertebrae can successfully avoid the operation scar, reduce the surgical complications and obtain satisfactory clinical outcomes.
Adult ; Aged ; Aged, 80 and over ; Endoscopy ; methods ; Female ; Humans ; Lumbar Vertebrae ; surgery ; Male ; Middle Aged ; Minimally Invasive Surgical Procedures ; Reoperation ; Spinal Fusion ; methods