Objective To study the effects of the IL-1 receptor antagonist (IL-1Ra) on matrix metalloproteinase 1 (MMP-1) expression in UV-irradiated fibroblasts. Methods Simulating the impact of environmental ultraviolet (UV) light on human skin, UVA-irradiated human fibroblasts were cultured in medium obtained from UVB-irradiated HaCaT cells. MMP-1 was detected by ELISA in the culture medium of fibroblasts. After treatment with IL-1Ra, the mRNA expression levels of C-Jun, C-Fos and GAPDH (internal control) of fibroblasts were measured by real-time fluorescent quantitative RT-PCR. Results Production of MMP-1 by UVA (10 J/cm2)-irradiated fibroblasts was increased in culture medium from UVB-irradiated HaCaT cells. The fibroblasts produced significantly higher levels of MMP-1 in culture medium from HaCaT cells treated without UVB than those with 15 mJ/cm2 UVB (t = 8.413,P= 0.014). However, IL-1Ra inhibited MMP-1 production of fibroblasts in a dose-dependent manner. Standard curves of real-time fluorescent quantitative RT-PCR showed a linear correlation between the copy number and the threshold cycle (Tc). Melting curves confirmed the specificity of PCR products. The original copy numbers of C-Jun and C-Fos as well as the ratios of the numbers to the GAPDH copy number showed that IL-1Ra inhibited the C-Jun mRNA expression of fibroblasts in a dose-dependent manner but had no significant effects on C-Fos mRNA expression. Conclusions The culture medium from UVB-irradiated HaCaT cells can promote MMP-1 production by UVA-irradiated fibroblasts. IL-1Ra reduces MMP-1 production via inhibition of C-Jun mRNA expression.

AIM: To investigate the effect of puerarin on expression of the glucogen synthase kinase-3 bate (GSK-3?) in adipocyte of rats with insulin resistance induced by diets. METHODS: 30 Wister rats were randomized into two groups: control group (n=10) and model group (n=20).Control group were fed with general diets, while model group were fed with the high fat diets to induce insulin resistance. After 4 weeks, the models have formed then were randomized into two groups: model group (n=10) and puerarin group (n=10). Puerarin group were treated with puerarin (100 mg?kg -1 ?d -1 , ip) for 6 weeks.GSK-3? protein was detected by Western-blot analysis. Body weight, serum triglyceride and cholesterol, fasting plasma glucose and serum insulin concentration were detected termly and the insulin sensitivity index was calculated. RESULTS: The model group showed hyperglycemia, hyperinsulinism, obviously obesity and the insulin resistance index (ISI) decrease by high fat diets, insulin resistance was induced by this way. Compared with model group, GSK-3? protein in adipocyte of puerarin group rats decreased (P

Objective To investigate the diagnosis of mesenteric vascular diseases by two-dimensional ultrasonography and color Doppler flow imaging (CDFI) and to summarize ultrasonographic imaging features.Methods Eighteen patients were examined by ultrasonography in emergency.The thickness,scope and echo of the wall of the small intestine,the condition of intestinal peristalsis,the blood flow of the wall of the bowel and the mesentery were measured by ultrasonography.Results The diagnosis of all the cases was confirmed by operation.The accuracy rate of ultrasonographic examination was 83.3% (15/18).Two patients were pseudo-positive( 11.1% ) and one patient was pseudo-negative( 5.6% ).Conclusions The ultrasonographic examination with directimaging features can determine the diagnosis of the disease and assess the classification and scope of mesenteric vascular diseases,hence is the first choice and an indispensable method.

Objective To investigate the effects of soybean isoflavones on cognition and expression of NMDA receptor (NR2B) subtype in hippocampus in Alzheimer's disease (AD) rats induced by amyloid ?-peptide 25-35(A? 25-35).Methods 60 female Wistar rats were randomly divided into five groups: model group, low dose of soybean isoflavone treatment group, high dose of soybean isoflavone treatment group, estrogen group and control group. AD models were made by injection A? 25-35 into bilateral hippocampus and normal saline was used in control group. Different dose of soybean isoflavone and estrogen were administered in soybean isoflavone treatment groups and estrogen treatment group for 18 d, respectively. The praxiology of rats was assessed by Morris water maze and the expression of NR2B in hippocampus was detected by immunohistochemistry method.Results Compared with control group,the learning and memory ability of model group obviously damaged and the expression of NR2B in hippocampus decreased (all P

Aorta, Thoracic ; abnormalities ; Humans ; Infant ; Magnetic Resonance Angiography ; Male ; Pulmonary Artery ; abnormalities ; Recurrence ; Respiratory Insufficiency ; etiology ; Respiratory Sounds ; etiology ; Subclavian Artery ; abnormalities ; Vascular Diseases ; complications ; congenital ; diagnosis ; pathology ; physiopathology

Objective:To investigate the value of preoperative slight decline of the pulmonary function in predicting postoperative cardiopulmonary complications (PCC) after left total pneumonectomy of lung cancer patients to guide the clinical surgery of lung can-cer. Methods:Clinical data of 200 lung cancer patients after left total pneumonectomy were retrospectively analyzed. These patients were divided into two groups (PCC and non-PCC) based on the incidence of postoperative cardiopulmonary complications within 1 month after the cancer resection. The relationship between the preoperative slight or moderate to severe reduction of pulmonary func-tion and postoperative complications was explored. The correlation between the preoperative slight decline of the pulmonary function index and PCC was also analyzed. Results:Among the 200 patients, 35, 45, and 120 demonstrated normal, slightly, and moderately to severely reduced pulmonary functions, and 28.6%, 53.3%, and 62.5% showed PCC within 1 month after the surgical resection (χ2=12.611, P=0.002). Significant differences in the slightly reduced pulmonary function parameters MVV% (70% ≤ MVV<80%) and FEV1%(70%≤FEV 1%<80%) were observed between those without and with complications (P=0.028 and 0.014). Further analysis indicated no significant difference between the patients with preoperative slight reduction of FEV1%only or of MVV%only (P>0.05). However, statistically significant differences were observed when both FEV1%and MVV%were slightly reduced (χ2=6.598, P=0.010). Conclusion:The preoperative slight reduction values of FEV1%and MVV%were the risk factors in predicting PCC after left pneumo-nectomy in lung cancer patients. The slightly reduced FEV1%or MVV%alone cannot influence PCC. Both slightly reduced FEV1%and MVV%were the risk factors of PCC. Perioperative management should be strengthened for these patients to lower the incidence of PCC.

Objective To investigate the relationship between COX-2 and Ki-67 proliferation index, bax, microvascular density(MVD)and CD44v6 respectively. Methods The expression of COX-2,Ki-67, bax, MVD (labelled by CD34) and CD44v6 were examined by immunohistochemistry PV method and tissue chip method in 101 cases of cervical carcinomas epithelium. Results The expression of COX-2 in cervical carcinomas has positive correlation with Ki-67, MVD and CD44v6, but has no correlation with bax. Conclusion The probable mechanisms are related with neovascularization, cell proliferation and cell adherence, but not related with the expression of bax.

This paper reviews the basic principles and calculation methods of MR specific absorption rate, discusses the clinical application of MR specific absorption rate.
Humans ; Magnetic Resonance Imaging ; Radio Waves

)0.05).Conclusions There is a predominant expression of Th_2-type cytokines in PBMC in PD patients. Abnormal immune function is one of the mechanisms involved in the pathogenesis of PD.

Objective To investigate the effects of Polysaccharide Sulfate (PSS) on plasma von Willebrand factor (vWF) and thrombomodulin (TM) in patients with acute cerebral infarction.Methods 64 patients with acute cerebral infarction were randomly assigned to receive intravenous drip of PSS (150 mg/d for 14 days, 32 cases, PSS group) or Troxerutin (600 mg/d for 14 days, 32 cases, control group). All of the patients were tested for plasmatic levels of vWF and TM prior to and at 7 d, 14 d after treatment.Results The levels of vWF in PSS group at 7 d, 14 d after treatment were obviously decreased compared with control group (all P