1.Expression of Human Interferon-λ1 and Interferon-ε Gene in WI-38 Cells and Comparison of Their Biological Activity
Bhushan SARODE ; Weihua SHENG ; Yufeng XIE ; Yaodong ZHAO ; Jingcheng MIAO ; Jicheng YANG
China Biotechnology 2007;27(3):24-33
The biological activities i. e. antineoplastic activities and antiviral activity of the two novel kinds of interferons: hIFN-λ1 and hIFN-ε were studied and compared. First the fusion expression vector: pcDNA3.1A-hIFN-λ1-His and pcDNA3.1A-hIFN-ε-His by PCR was constructed, then the two kinds of plasmids were transfected into WI-38 (human embryonic lung cells ) with liposome. And cytopathic effect (CPE) suppression test was used to study and compare the antiviral activities of rhIFNλ1-His and rhIFN-ε-His, meanwhile MTT assay was used to detect their antineoplastic activities. It was found that, antiproliferative activity and MxA protein induction shown by rhIFN-λ1-His is more powerful than of rhIFN-ε -His. The antiviral molecular mechanisms of both hIFN-λ1 and hIFN-ε are related to MxA. The foundation for further study on the bioactivities and mechanism of action of hIFN-λ1 and hIFN-ε was established.
2.Siderophoregenic Acinetobacter calcoaceticus isolated from wheat rhizosphere with strong PGPR activity
Sarode Prashant D ; Rane Makarand R ; Chaudhari Bhushan L ; Chincholkar Sudhir B
Malaysian Journal of Microbiology 2009;5(1):6-12
Thirty-two bacterial isolates were obtained from wheat rhizosphere in black cotton soils of North Maharashtra region and subsequently tested for in-vitro siderophore production. Wheat isolate SCW1, being a strong siderophore producer, was selected, identified and confirmed as Acinetobacter calcoaceticus. The strain produced catechol type of siderophores during exponential phase which was influenced by iron content of medium. Seed bacterization with siderophoregenic A.calcoaceticus improved plant growth in pot and field studies. Such PGPR activity was attributed to the ability of strain to
solubilise phosphates and produce IAA. Siderophore mediated antagonism was observed against common
phytopathogens viz., Aspergillus flavus, A. niger, Colletotrichum capsicum and Fusarium oxysporum
3.Expression of Human Interferon-?1 and Interferon-? Gene in WI-38 Cells and Comparison of Their Biological Activity
Bhushan SARODE ; Wei-Hua SHENG ; Yu-Feng XIE ; Yao-Dong ZHAO ; Jing-Cheng MIAO ; Ji-Cheng YANG ;
China Biotechnology 2006;0(03):-
The biological!activities i.e. antineoplastic activities and antiviral activity of the two novel kinds of interferons: hIFN-?1 and hIFN-? were studied and compared. First the fusion expression vector: pcDNA3.1A-hIFN-?1-His and pcDNA3.1A-hIFN-?-His by PCR was constructed,then the two kinds of plasmids were transfected into WI-38 (human embryonic lung cells) with liposome. And cytopathic effect (CPE) suppression test was used to study and compare the antiviral activities of rhIFN-?1-His and rhIFN-?-His, meanwhile MTT assay was used to detect their antineoplastic activities.It was found that, antiproliferative activity and MxA protein induction shown by rhIFN-?1-His is more powerful than of rhIFN-?-His. The antiviral molecular mechanisms of both hIFN-?1 and hIFN-? are related to MxA.The foundation for further study on the bioactivities and mechanism of action of hIFN-?1 and hIFN-? was established.
4.Expression of Human Interferon-λ1 and Interferon-ε Gene in WI-38 Cells and Comparison of Their Biological Activity
Bhushan SARODE ; Wei-Hua SHENG ; Yu-Feng XIE ; Yao-Dong ZHAO ; Jing-Cheng MIAO ; Ji-Cheng YANG
China Biotechnology 2007;27(3):24-33
The biological activities i. e. antineoplastic activities and antiviral activity of the two novel kinds of interferons: hIFN-λ1 and hIFN-ε were studied and compared. First the fusion expression vector: pcDNA3.1A-hIFN-λ1-His and pcDNA3.1A-hIFN-ε-His by PCR was constructed, then the two kinds of plasmids were transfected into WI-38 (human embryonic lung cells ) with liposome. And cytopathic effect (CPE) suppression test was used to study and compare the antiviral activities of rhIFNλ1-His and rhIFN-ε-His, meanwhile MTT assay was used to detect their antineoplastic activities. It was found that, antiproliferative activity and MxA protein induction shown by rhIFN-λ1-His is more powerful than of rhIFN-ε -His. The antiviral molecular mechanisms of both hIFN-λ1 and hIFN-ε are related to MxA. The foundation for further study on the bioactivities and mechanism of action of hIFN-λ1 and hIFN-ε was established.