OBJECTIVE: Screen seventeen benzodizepines and their metabolitesin urine by GC/ECD and GC/MS. METHODS: They were GC (GC/ECD, GC/MS) assay of benzodizepines and GC (GC/ECD, GC/MS) assay of benzophenones of acid-hydrolytic products of 1,4-benzodizepines. RESULTS: The methods were simple and sensitive. The recoverys were 60% to 90% of most benzodizepines, linear calibration curves were 20 ng/ml-200 ng/ml (r > 0.99), and detection limits were 0.5 ng/ml-10 ng/ml. CONCLUSION They methods were evaluated with human urine samples.
Anti-Anxiety Agents/urine* ; Benzodiazepines/urine* ; Benzophenones/urine* ; Chromatography, Gas ; Gas Chromatography-Mass Spectrometry ; Humans

AIMTo investigate the metabolic pathways of dipfluzine in rats.

METHODSAfter an oral dose of dipfluzine (80 mg x kg(-1)) to rats, urine was collected for 12 h. The metabolites of dipfluzine in urine were chromatographed and identified by LC/DAD/MS methods.

RESULTSIn the rat urine, there were 1-(4-fluorophenyl)-4-piperazinylbutanone and its glucuronide, 4-hydroxybenzophenone and its glucuronide, 4-fluoro-gamma-hydroxybenzenebutanoic acid and its glucuronide and sulfate, diphenylmethanol and its glucuronide, dipfluzine, and benzophenone.

CONCLUSIONIn rats, dipfluzine was mainly metabolized in the pathways of N-desalkylation at 1- and 4-positions of piperazine ring. Some of metabolites were further conjugated with glucuronic acid and/or sulfuric acid.

Animals ; Benzophenones ; urine ; Chromatography, Liquid ; Cinnarizine ; analogs & derivatives ; metabolism ; urine ; Female ; Gas Chromatography-Mass Spectrometry ; Glucuronides ; urine ; Male ; Rats ; Rats, Wistar