IL-8 was radiolabeled with 125I via Bolten-Hunter agent and the distribution of 125I-IL-8 in mice was determined in order to understand IL-8 behaviors in vivo. The percentage of 125I-IL-8 in blood, heart, liver, lung, kidneys, bone and spleen was obtained. The half-clearance of fast phase T1/2 alpha and slow phase T1/2 beta were 0.32 h and 8.01 h respectively. Most of 125I-IL-8 was excluded by kidneys.
Animals ; Interleukin-8 ; pharmacokinetics ; Iodine Radioisotopes ; Male ; Mice ; Recombinant Proteins ; pharmacokinetics ; Tissue Distribution

Objective: To investigate the situation and influencing factors of bowel cleansing in patients before colonoscopy, and to provide reference for guiding patients′ bowel preparation. Methods: The clinical data of 421 patients undergoing electronic colonoscopy in the endoscopy center of the second people′s hospital of Lu′an city in Anhui Province from April to September 2018, Prospective collection by systematic sampling, including general data of patients, clinical data of bowel preparation and score of Boston bowel preparation scale. Univariate analysis and logistic regression analysis were performed on the relevant factors of bowel preparation. Results: Among the 421 patients, 52 cases were not eligible for intestinal cleaning, and the unqualified rate was 12.35%(52/421). Logistic regression analysis showed that: Total amount of drinking water prepared for intestinal tract before colonoscopy (OR=1.674, P=0.018),The total times of defecations after taking the drug (OR=1.270, P=0.014), the characteristics of last stool before colonoscopy (OR=2.211, P=0.042), the diet the day before colonoscopy (OR=0.553, P=0.006) , body mass index (OR=1.111, P=0.042), and intestinal diseases (P=0.022), were all intestinal clearance independent influencing factors of cleaning effect; While took laxative type (mannitol/PEG), drunk water speed (within 3 hours), first defecation time, the site of drinking bowel-clearing drugs, daily activity, none of the above factors affected the bowel preparation effect (P>0.05) . Conclusion Before colonoscopy, there were many factors affecting the cleaning effect of the intestine. The total water intake of intestinal preparation was less than 2 000 ml, the total number of defecation after taking medicine was less than 5 times, the last defecation contained residue, the day before the examination was strictly fasting, the body mass index was less than 18.5 kg/m², and colitis, enterelcosis and occupying lesion are independent risk factors for intestinal cleaning. Attention should be paid to the comfort care and guidance of intestinal preparation, and prospective intervention can effectively improve the cleaning effect of intestinal tract.