AIM: To investigate the chemical constituents of Shuanghuanglian Injection(Flos lonicerae japonicae, Radix scutellariae, Fructus forsythiae). METHODS: Chromatography and spectral analysis were used respectively to isolate and identify the constituents. RESULTS: Six compounds were isolated from Shuanghuanglian Injection which included baicalin(Ⅰ)、quercitrin(Ⅱ)、hyperoside(Ⅲ)、rutin(Ⅳ)、quercetin(Ⅴ) and luteolin(Ⅵ). CONCLUSION: The other five compounds were first isolated and identified in Shuanghuanglian Injection except baicalin(Ⅰ).