Objective To investigate the role of lncSIL in transforming growth factor-β1(TGF-β1)-induced alveo-lar epithelial interstitial transformation(EMT)and its related signaling pathways.Methods Western blot was used to detect the effect of lncSIL silencing on the expression of E-cadherin(E-cad),alpha-smooth muscle actin(α-SMA)and Collagen I(Col I)in the process of EMT induced by TGF-β1.LncSIL interacting proteins were ana-lyzed by RNA pulldown.Western blot was used to detect the effect of overexpression or silencing of lncSIL on the expression of its target gene enhancer of zeste homolog 2(EZH2)and its downstream factors P21 and cyclin-de-pendent kinase 6(CDK6).Flow cytometry was used to analyze the effect of lncSIL on cell cycle progression.Re-sults After lncSIL silencing,the expression of α-SMA and Col I increased,the expression of E-cad decreased.RNA pulldown assay showed that EZH2 was the target protein that interacted with lncSIL,and the expression of EZH2 increased after silencing lncSIL,the expression of EZH2 downstream gene P21 decreased,CDK6 increased.Flow cytometry showed that the number of cells in S phase significantly increased.When lncSIL was overexpressed,the expression of EZH2 and CDK6 was down-regulated,the expression of P21 was up-regulated,and the number of S phase cells significantly decreased.Conclusion LncSIL inhibits TGF-β1-induced alveolar epithelial cell mesen-chymal transition by negatively regulating EZH2/P21/CDK6 signaling pathway to inhibit cell cycle progression.

Objective To investigate the value of preoperative intravoxel incoherent motiondiffusion weighted imaging (IVIM-DWI) in predicting early recurrence of hepatocellular carcinoma (HCC)after curative hepatectomy.Methods The clinical data of 51 HCC patients who underwent curative hepatectomy at Beijing Tsinghua Changgung Hospital,Tsinghua University from December 2014 to March 2017 were retrospectively analyzed.The study included 45 males and 6 females,aged 56.4 ± 10.1.The patients were divided into the early-recurrence group (21 patients) and the non-recurrence group (30 patients) according to whether there was HCC recurrence within 1 year after curative hepatectomy.The parameters of the lesions were measured and calculated:the apparent diffusion coefficient (ADC) value,true diffusion coefficient (D) value,perfusion-related diffusion coefficient (D *) value and perfusion fraction (f) value.Receiver operating characteristic curves (ROC) were used to evaluate the prediction efficiency of the parameters.Results The ADC and D values of the early-recurrence group were significantly lower than the non-recurrence group.The differences were statistically significant (P ＜ 0.05).In predicting early recurrence of HCC after curative hepatectomy,the ADC values showed the area under ROC was 0.713 (95％ CI:0.572 ～0.855),the sensitivity was 0.857 and specificity was 0.567 when the optimal threshold value was 1.24 × 10-3mm2/s.The D values in predicting early recurrence demonstrated the area under ROC was 0.740 (95％ CI:0.602 ～ 0.877),the sensitivity was 0.905 and specificity was 0.600 when the optimal threshold value was 1.03 × 10-3 mm2/s.Conclusions The ADC and D values of IVIMDWI could provide evidence in predicting early recurrence of HCC after curative hepatectomy.The D values had a higher prediction efficiency.