Objective To investigate the drug resistance and distribution of Staphylococcus aureus isolated from infected pa‐tients in our hospital from 2009 to 2013 ,and provide basis for clinical treatment .Methods Retrospective review was adopted to an‐alyze the antibiotic resistance and the specimen source of 562 SA strains .ATB Expression and ID 32 STAPH were used to identify SA .Antibiotic susceptibility testing was performed by K‐B method .Results 562 SA strains ,including 218 MRSA ,are mainly from secretions ,sputum and pus .Resistance was most frequently observed on penicillin ,followed by erythromycinand clindamycin .None of the isolates was resistant to vancomycin ,amikacin ,nitrofurantoin and linezolid .The resistance rates of MRSA to penicillin ,eryth‐romycin ,cotrimoxazole ,clindamycin ,gentamicin ,cefoxitin ,tetracycline and rifampicin were obviously higher than that of MSSA ,and there are very significant differences between them (P<0 .05) .Conclusion There is a declining trend in the isolation rates of SA and MRSA in our hospital ,but the drug resistance situation remains serious .Vancomycin is still the first option for the cure of MR‐SA infections .Therefore ,strengthening SA resistance monitoring and avoiding misuse of antimicrobial drugs is an effective way to prevent SA infection .