Objective: To study the long-term effect of Tangshan earthquake on psychosomatic health of paraplegic suffers. Method: 64 paraplegic suffers of Tangshan earthquake and 64 normal controls were interviewed and assessed with self made questionnaire for psychosomatic health, SCL-90, SAS, SDS, CMI (Cornell Medical Index) and SSRS (Social Support Rating Scale). Results: 6 patients (9%) were diagnosed as PTSD according to CCMD-2-R, this rate was higher than that of normal citizen experienced the earthquake. 32 patients had Acute Stress Reaction. At present, patients' group had poorer mental health than control reflected by SCL-90, SAS, SDS and CMI. Conclusion: The severity of trauma both mentally and physically has great influence on mental health of suffers even after 25 years.

Objective:To investigate if a plant-derived polysaccharide sulfate(M33A) binding to HIV1 gp120 may induce the exposure of neutralization epitopes of gp120,and if M33A-bound HIV-1ⅢB antigens may be used as a AIDS vaccine for inducing neutralizing antibodies against HIV-1.Methods:Whole-inactivated M33A-bound HIV-1ⅢB antigens were prepared and used to immunize mice after mixing with FCA or FIA.The titers of anti-HIV-1 IgG antibodies in immunized mouse plasma were detected by ELISA,and the HIV-1 neutralization by those plasma was detected by the improved microtiter neutralization assay.Results:M33A-bound HIV-1 antigens induced higher titers of anti-HIV-1 IgG antibodies(group C:1.5?10~6;group D:1.5?10~6) than HIV-1 antigens alone(4.9?10~5),and female mice produced 3 times higher titers of anti-HIV-1 IgG antibodies than male mice after immunized with various HIV-1 antigens.All three immunization schemes did not induce the production of anti-HIV-1 neutralizing antibodies.Conclusion:M33A binding does not induce gp120 to expose neutralization epitopes.However,M33A may improve the level of mice immune responses to HIV-1 antigens,suggesting M33A may enhance immune response to HIV-1 antigens.

Silent information regulator 1 (SIRT1) is a NAD+-dependent histone deacetylase,which plays an important role in cerebral ischemia.It mainly stabilizes cerebral vascular endothelium,prevents vascular stenosis and the anti-inflammatory and anti-oxidative stress effects after cerebral ischemia.This article reviews the protective effects of STRT1 and its related mechanisms in cerebral ischemia.

Objective To explore whether the inhibited expression of fibrocystin by RNA interference can increase epidermal growth factor (EGF)-induced cell proliferation and its possible mechanism . Methods A stable PKHD1-silenced HEK 293 cell line was established . Cell proliferation rate, intracellular Ca2+ concentration and extracellular signal-reguhted kinase 1/2(ERK1/2) activity were assessed after treatment with EGF, verapamil and Bay K8644 . Results The proliferation rate of PKHD1-silenced HEK-293 cells was found to be significantly higher after EGF stimulation compared to the control HEK 293 cell (231 .5% vs 152 .8%, P＜0 .01) . PKHD1-silencing lowered the intracellular Ca2+ concentration and caused EGF-induced ERK1/2 overactivation in the cells(P＜0 .01 ) . When cells were treated with verapamil for 4 hours to lower the intracellular Ca2+ concentration, the cell proliferation rate was significantly increased after 20 ng EGF for 24 hours . The verapamil treatment increased the level of activated ERK1/2 in EGF-treated cells . An increase of intracellular Ca2 + in PKHD1-silenced ceils repressed the EGF-dependent ERK1/2 activation and the hyperproliferative response to EGF stimulation . Conclusions Inhibition of fibrocystin can cause EGF-induced excessive proliferation through decreasing intracellular Ca2+ resulting in EGF-induced ERK1/2 activation . The loss of fibrocystin may lead to abnormal proliferation in kidney epithelial cells and cyst formation in ARPKD through modulation of intracellular Ca2+ concentration .

Conopeptides (Conotoxin) derived from tropical marine gastropod, cone snail ( Conus ) venoms have been an useful tool agent in neuro investigation and new drug source. Recently, new method of Conotoxin genes cloning from Conus genomic DNA has been established. So to get Conus genomic DNAs rapidly is the basis for diverse Conotoxin gene isolation, gene bank construction and the medical source exploitation. In this report, different DNA extractions from different tissue and organs of six cone snail species were performed in order to optimize the DNA isolation methods. The optimized brief phenol SDS method was established which fit for cone snails genomic DNA extraction.

Objective To observe the polysomnographic manifestation of sleep disorder and the characteristics of sleep architecture in patients with PD.Methods 42 patients with PD and 40 normal controls underwent night polysomnography.The parameters of sleep architecture and progress in two groups and video-monitoring features were analyzed.Results According to PSG recordings,the incidence of difficulties in the initiation of sleep(73.8%),fragmented sleep(59.5%),excessive daytime sleepiness (46.1%)were respectively increased in PD patients group than that in controls(all P5)、 ESS were increased(P=0.022,0.000,0.007, 0.001,0.000,respectively).SOREMPs occurred in 6 patients(14.3%)in PD group,but didn't oecure in controls.In PD group REM without atonia(RWA)was demonstrated in 36 patients(85.7%),RBD in 19 patients;in control group,however,REM in 6 and RBD in 2 separately.The statistics analysis showed the incidence of RWA(85.7%)and RBD(45.2%)in PD group was significantly higher than that in control group(P

Objective To detect the relationship between CAG repeat in IT15 gene and clinical manifestations of Huntington's disease (HD).Methods Non-denaturing polyacrylamide gel electro- phoresis method was used to detect CAG repeat.Clinical manifestations were scored by UHDRS and MMSE. Results Genotypes of IT15 were heterozygous in all 29 HD patients.CAG repeat in the HD chromosome, being 42—62,13—28 in normal chromosome,was inversely correlated with age of onset (r=-0.539,P

Objective:To explore the relative factors of hospitalized schizophrenics with metabolic syndrome. Methods: Patients data were collected by the way of face-to-face questionnaire to patients or their guardians, consulting patients medical record, checking physical or laboratory examination. Information include age, duration of the disease, the classification of antipsychotics and dosage, fast plasma glucose, plasma lipids, blood pressure, etc of hospitalized schizophrenics. The relative factors of metabolic syndrome were analyzed. Results; Among 797 schizophrenics, there were 153 cases (19. 2% ) suffered from metabolic syndrome. Single-factor analysis showed that the patients with longer duration of the disease, taking clozapine, etc, were at high risk of metabolic syndrome, and sex didnt relate to metabolic syndrome; Logistic regression analysis appeared that metabolic syndrome correlated with age, clozapine, CRP concentration, etc. Conclusion: The possible risk factors causing metabolic syndrome in schizophrenia include, duration of the disease, etc.

Objective To evaluate the diagnostic and therapeutic status of lower urinary tract symptoms (LUTS) of benign prostatic hyperplasia (BPH) in urological outpatients.Methods The survey was conducted at 57 urological clinics in 14 cities during the period of October-December 2011.All surveyed male outpatients were inquired about whether there was a history of LUTS/BPH or not.Then eligible patients received a more detailed questionnaire for LUTS.Results Among a total of 6 200 male outpatients,47.4％ (n =2 940) had a history of LUTS/BPH.The rate for LUTS was 30.0％ (n =882) in patients aged under 40 years and 60.0％ (n =1 764) in those aged over 60 years.The major complains included frequency (72.0％),urgency (53.0％) and nocturia (48.0％).At a rate of 63.0％,the patients aged 71-80 years came more to a hospital for nocturia than 61-70 years (52.0％) and 51-60 years (49.0％).The average International Prostate Symptom Score (IPSS) was 14.98 (6-35) and 59.0％ patients had moderate symptoms and 26.0％ severe symptoms.The average score of quality-of-life was 4.12 (1-6) points.And the average overactive bladder symptom score (OABSS) was 5.78 (3-15) points and 56.0％ patients had moderate overactive bladder (OAB) and 3.0％ severe OAB.Urinalysis was largely normal.The main causes for LUTS included OAB (25.2％),BPH and OAB (20.4％),BPH (20.2％),prostatis (20.2％) and urinary infection (11.8％).Conclusions LUTS and BPH are common in urological outpatients.And the incidence is rising yearly.Clinical examinations and drug regimens should be timely adjusted according to the etiology.And patient education should be strengthened at the same time.

Objective To evaluate the effect of hydrodistension on interstitial cystitis and the psychological changes after hydrodistension.Methods Thirty two patients with interstitial cystitis underwent hydrodistension from May 2011 to Jun 2012 in Department of Urology,Beijing Hospital.Interstitial cystitis symptom index(ICSI),Visual onalogue Scale (VAS) pain score,quality of life questionnaire(QOL) score,self-rating anxiety scale(SAS) score,self-rating depression scale(SDS) score were recorded and analyzed before and at 1-3 month after hydrodistension surgery.The voiding diary and postoperative complications were cared.Results Symptoms were improved in twenty nine patients,the efficiency rate was 90.6％ (29/32).There were significant differences in ICSI score,VAS pain score,QOL score,SAS score,SDS score between pre and 3 month post-surgery [(26.7± 2.3) vs.(15.2±1.9),(4.6±2.5) vs.(1.8±1.1),(4.3±2.2) vs.(2.2±1.6),(53.8±3.7) vs.(40.8±2.3),(54.8±2.3) vs.(42.4±1.5),t=8.33,7.94,6.54,7.20,8.52,respectively,all P ＜0.05].No complication was found after surgery.Both the clinical symptoms and the anxiety and depression symptoms in patients were relieved,and QOL was improved.Conclusions For the patients with interstitial cystitis,the undergoing of hydrodistension surgery can relieve the anxiety and depression symptoms and improve quality of life.