OBJECTIVE:To develop a new way for the extration and determination of naringin in Davallia mariesii. METH-ODS:Naringin in D. mariesii was extracted by ASE350 accelerated solvent extraction system;HPLC was performed for the con-tent determination of naringin in D. mariesii,the column was Kinetex XB-C18 with mobile phase of methanol-5%acetic acid(25∶75, V/V)at a flow rate of 0.8 ml/min,detection wavelength was 283 nm,column temperature was 40℃,and injection volume was 10 μl. RESULTS:The linear range of naringin was 0.073 0-0.730 0 μg(r=0.999 9);the limit of quantitation was 0.73 μg,the limit of detection was 0.022 μ g;RSDs of precision, stability and reproducibility tests were lower than 2% ;recovery was 98.92%-100.85%(RSD=0.72%,n=6). CONCLUSIONS:The method is simple,accurate and specific,and can be used for the content determination of naringin in D. Mariesii.