AIM　DDT1 MF-2 hamster smooth muscle cells were used to investigate the role of α1B-adrenoceptor (AR) in the cell proliferation and its signaling pathway. METHODS　DNA synthesis was measured by ［3H］thymidine (TdR) incorporation and the cell cycle was determined by flow cytometry. The actions of several inhibitors and activators of second messenger on NE-induced DNA synthesis were investigated. RESULTS　NE (0.1～1 μmol*L-1) elicited significant concentration-dependent stimulation of DDT1 MF-2 cell proliferation. The proliferative effect caused by α1B-AR was blocked by PLC inhibitor (U73122, 10 μmol*L-1), Ca2+/ATPase inhibitor (cyclopiazonic acid, 10 μmol*L-1), intracellular Ca2+ chelator (BAPTA/AM, 10 μmol*L-1), PKC inhibitors (RO-31-8220, 0.1 μmol*L-1 and calphostin C, 0.1 μmol*L-1), TK inhibitors (tyrphostin A25, 10 μmol*L-1 and genistein, 10 μmol*L-1), and MEK1/2 inhibitor (PD 98059, 10 μmol*L-1). CONCLUSION　α1B-AR subtypes stimulate DDT1 MF-2 cells growth and its signal pathway is related to the PLC activation、Ca2+ release、PKC、TK and ERKs activation.

AIM DDT1 MF 2 hamster smooth muscle cells were used to investigate the role of ? 1B adrenoceptor (AR) in the cell proliferation and its signaling pathway. METHODS DNA synthesis was measured by [ 3H]thymidine (TdR) incorporation and the cell cycle was determined by flow cytometry. The actions of several inhibitors and activators of second messenger on NE induced DNA synthesis were investigated. RESULTS NE (0 1～1 ?mol?L -1 ) elicited significant concentration dependent stimulation of DDT1 MF 2 cell proliferation. The proliferative effect caused by ? 1B AR was blocked by PLC inhibitor (U73122, 10 ?mol?L -1 ), Ca 2+ /ATPase inhibitor (cyclopiazonic acid, 10 ?mol?L -1 ), intracellular Ca 2+ chelator (BAPTA/AM, 10 ?mol?L -1 ), PKC inhibitors (RO 31 8220, 0 1 ?mol?L -1 and calphostin C, 0 1 ?mol?L -1 ), TK inhibitors (tyrphostin A25, 10 ?mol?L -1 and genistein, 10 ?mol?L -1 ), and MEK1/2 inhibitor (PD 98059, 10 ?mol?L -1 ). CONCLUSION ? 1B AR subtypes stimulate DDT1 MF 2 cells growth and its signal pathway is related to the PLC activation、Ca 2+ release、PKC、TK and ERKs activation.

Objective To investigate the intervention effect of motivational interviewing health education based on trans-theoretical model to hospitalized patients with cerebral infarction on their healthy diet cognition and behavior change.Methods 60 patients with cerebral infarction were selected with convenient sampling method,and in accordance with the incorporating order randomly divided them into the intervention group and the control group,each with 30 cases.The intervention group received the motivational interviewing health education based on the trans-theoretical model,which mainly focused on a healthy diet,while the control group received regular health diet education.The intervention effect was compared between two groups.Results The comparative difference of baseline data between the two groups before intervention had no statistical significance,while after intervention the change on knowledge and attitude of these two groups both had statistical significance,but the intervention group improved more obviously than the control group; In regards to behavior change phrase,comparing the healthy eating behavior phase of the intervention group patients before and after the intervention,it was found that before the intervention only 4 cases were on the stage of about-to-change,accounting for 13.3％,but after the intervention the proportion was up to 40.0％ with 12 cases.Using chi-square test to compare the behavior phrase difference of the patients before and after the intervention,the difference was statistically significant.Conclusions Motivational interviewing technique based on the trans-theoretical model is an effective method in terms of cognitive and behavioral change,and the health education will improve the cognition on healthy diet and the behavior of the hospitalized patients with cerebral infarction.

Objective To investigate the application of radial endobronchial ultrasound (R-EBUS)-guided bronchoscopy in the diagnosis of peripheral pulmonary lesions (PPLs),assess its safety and diagnostic value,and explore the influencing factors.Methods Clinical data of 140 patients who underwent R-EBUS transbronchial biopsy (TBB) and brush biopsy in our department of respiratory endoscopy from January 2015 to March 2017 were collected and retrospectively analyzed in the study.Eighty-three cases were diagnosed as PPLs.The detection rate,ability to locate the peripheral lesions,and influencing factors of EBUS were analyzed.The incidence rate of complications was observed to assess its safety.Results Of the 83 PPLs patients,they were 55 males and 28 females,and at a mean age of 59.81 ± 11.85 years.The total success rate of EBUS-guided bronchoscopic diagnosis was 59.04％ (49/83),and the diagnostic rate was 50.94％ (27/53) for malignant lesions,and 73.33％ (22/30) for benign diseases.The rate of EBUS-TBB (56.92％,37/65) was significantly higher than that of brush biopsy (30.12％,25/83,Chi square =10.76,P =0.001).EBUS-guided diagnostic methods had an accuracy of 60.00％ (45/75),while the EBUS-guided bronchoscopy had a rate of 50.0％ (4/8),but no significant difference was seen between the 2 methods (Chi square =0.03,P =0.87).The sizes of the lesions ranged from 10.0 to 52.4 mm,and the diagnostic yield for PPLs ≤20 mm in diameter (36.84％,7/19) was lower than that for those ＞20 mm (65.62％,42/64,Chi square =5.02,P =0.003).There was no significant difference (Chi square =10.05,P =0.07)in the diagnostic yield for different sites,with that of right upper lobe of 46.15％ (12/26),right middle lobe 100.00％ (8/8),right lower lobe 53.85％ (7/13),left upper lobe 37.50％ (3/8),left lingula lobe 63.64％ (7/11),left lower lobe 70.59％ (12/17).But the detection rate was obviously lower in the upper lobe (22/45,48.89％) than the middle/lower lobe (27/38,71.05％,Chi square =5.02,P =0.003).Of all the 83 patients,74 ones (89.16％) were successfully identified using radial probe EBUS,but the lesions were not found in 9 patients.When the radial probe position was within the target lesion,the diagnostic yield was 81.58％ (31/38),notably higher than that when the probe was positioned adjacent to the lesion (50.00％,18/36,Chi square =8.24,P =0.004).Mild bleeding was observed when performing biopsy under bronchoscope,and no pneumothorax,or other serious complications were observed.Conclusion Radial EBUS is a safe and feasible nethod to accurately identify PPLs and improve its diagnostic rate.EBUS-guided bronchoscopy has higher positive detection rate for PPLs,and is cost saving.The relationship of the probe and the site of lesion is the main factor influencing the diagnostic rate.

This paper reviewed the present nursing manager succession planning at home and abroad, covering the succession standard of nursing managers, the content and form of pre-job training, and the evaluation tool of management ability. These efforts aim to provide references for the construction of nursing management talents teams in China.

Objective To analyze the status and influencing factors of job satisfaction of nurses at public hospitals, providing references for the teambuilding strategy of nurses. Methods Questionnaire survey method was used, in October 2017, to study job satisfaction of 1,047 nurses at 13 public general hospitals, for a countermeasure study. Results Job satisfaction of the nurses was found at a medium level. Specifically, their overall job satisfaction score was 3. 68, and the satisfaction scores of the following dimensions from high to low were: on-the-job training (3. 91), hierarchical nurse use (3. 79), performance appraisal (3. 68), promotion evaluation (3. 67), and remuneration (3. 62) respectively. Contents of the five dimensions are strongly related to the overall job satisfaction (r＞0. 6). Job satisfaction scores of nurses with their differences in positions, annual income, and hospital level were significantly different (P＜ 0. 05). The three main influencing factors for talent turnover rate are remuneration, workplace pressure and work environment. Conclusions Higher remuneration, improved hierarchical management system, and greater attention to nursing care at secondary hospitals are very important for effectively improving the job satisfaction of nurses and ensuring the health care industry.

Objective　To investigate the clinical features and prognosis of anterior circulation cerebral infarction secondary to cervical artery dissection.Methods　Sixty one patients with cervical artery dissection (CAD) secondary to anterior circulation cerebral infarction treated in Department of Neurology of North Jiangsu People’s Hospital from January 2016 to December 2020 were included consecutively.And 67 patients with large artery atherosclerosis (LAA) cerebral infarction complicated with moderate to severe carotid artery stenosis who were hospitalized at the same time were enrolled for comparison.The clinical data and prognosis between the two groups were compared.Results　Unifactorial analysis showed that the average age of CAD cerebral infarction group was younger,the incidence of head and neck pain and monocular amaurosis was higher,the proportion of massage trauma fatigue history was higher,the incidence of hypertension and smoking history was higher in LAA cerebral infarction group.The comparison of the two hematologic examinations showed that the erythrocyte count was higher in the CAD cerebral infarction group,and the D-dimer,cholesterol,glycated hemoglobin,and uric acid values were higher in the LAA cerebral infarction group.Binary logistic regression analysis showed that the history of massage trauma fatigue was more correlated with CAD cerebral infarction,while older age and smoking history were more correlated with LAA cerebral infarction.The improvement of NIHSS scores during hospitalization in the CAD cerebral infarction group was more significant than that in the LAA cerebral infarction group,and the difference was statistically significant.There were no significant differences in 90 day prognosis,mortality,and reinfarction rate within 6 months between the two groups.Conclusion　The correlation between the history of massage trauma and fatigue and CAD cerebral infarction was greater,and the correlation between the older age and smoking history and LAA cerebral infarction was greater.The improvement of NIHSS score in CAD cerebral infarction group during hospitalization was more obvious than that in LAA cerebral infarction group,which was statistically significant.There were no significant differences in 90 days prognosis,mortality and 6 month reinfarct rate between the two groups.