Objective To investigate whether hepatocyte growth factor ( HGF ) and insulin-like growth factor (IGF1) induce cardiac stem cells (CSCs) to proliferate and directly differentiate into cardiomyocytes in vitro.Methods The myocardial tissues were dissected for primary culture of CSCs with the method of explants .The expressions of c-kit and CD34 were examined with immunofluorescence .Primary cells were purified with c-kit by flow cytometry.CFDA SE fluorescent probe was used to detect the proliferation of c-kit+CSCs.C-kit +CSCs were divided into two groups , and cardiac stem cells group and co-cultured with cardiomyocytes group , both group were cultured with HGF and IGF 1.An inverted microscope was used to observe changes in cell number and morphology in different periods .Living cells workstation was used to observe CFDA SE fluorescence intensity , to acquire images and do statistical analysis .Immunofluorescence technique was used to detect the expression of Nkx 2.5 and cardiac troponin T .Results In cardiac stem cells group ,CSCs had no obvious changes in cell number .In co-cultured with cardiomyocytes group , CSCs proliferated and had changes in morphology .Nkx2.5 and cTnT were positively expressed . Several CSCs differentiated into beating cardiomyocytes . Conclusion In co-cultured with cardiomyocytes condition , HGF and IGF1 may promote CSCs to proliferate and differentiate into beating cardiomyocytes .

AIM: To study the changes of bacterial ultrastructure, membrance potential and membrance patency of Escherichia coli during the postantibiotic effect after exposure to gatifloxacin and ciprofloxacin in order to investigate the mechanisms of PAE. METHODS: During the Postantibiotic effect after exposure to gatifloxacin and ciprofloxacin, the aliquots were taken from the bacterial culture at regular intervals. Then the fluorescence microscope was used to observe changes in bacterial ultrastructure and at the same time we studied the changes of membrance potential and membrance patency in Escherichia coli during the postantibiotic effect by flow cytometry in conjunction with fluorescent probes. RESULTS: The PAE phase characterized by filament formation after exposure to gatifloxacin by fluorescence microscopy,yet no significant changes in membrance potential and membrance patency of Escherichia coli were observed. CONCLUSION: Gatifloxacin and ciprofloxacin can induce filamentation, and this change is indifferent with membrance potential and membrance patency of Escherichia coli.

OBJECTIVE To control abuse use of antibiotics.METHODS Various methods were used to strengthen the management of antibacterials,for example,fully exert the rational use antibiotics,enforce the training of the doctors and nurses for the rational use of the antibiotics,and stipulate the rules and principles for the use of antibiotics.RESULTS The management of antibacterials was strengthened,and the rational use of antibiotics was improved.CONCLUSIONS To enhance surveillance of antibacterial use and prevent antibacterial unrational use are propitious to control occurrence of nosocomial infections.

OBJECTIVE To analyze the adverse drug reactions of 6 kinds of quinolones in order to provide information for clinically rational use of drugs.METHODS The adverse drug reactions of quinolones such as norfloxacin,ofloxacin,levofloxacin,ciprofloxacin,fleroxacin and rufloxacin used in outpatients during Feb to May 2007 were collected and retrospectively analyzed.RESULTS The main adverse drug reactions were seen in gastrointestinal system,nervous system,immune system,respiratory system,urinary system etc.From them the symptoms of gastrointestinal system and nervous system were prominent.CONCLUSIONS In use of quinolones it should think highly to their adverse drug reactions.

Objective To investigate the current state of nurse leaders′leadership and nurse physician cooperation, to explore the relationship between them, and to provide reference for improving nurse physician cooperation situation. Methods Totally 193 nurse leaders were recruited who were participated in Anhui nursing management training courses. A series of questionnaires were used to collect data, including general information, competency inventory for registered nurse, nurse physician cooperation scale. Results One hundred and eighty- two questionnaires were valid. The score of nurse managers′leadership was (27.85±6.27) points,while the score of nurse physician cooperation scale was (68.31±11.67) points. Hierarchical regression analysis showed that the control of general information, leadership for the interpretation of the nurse physician cooperation level increased by 27.3% (△R2 = 0.273, P = 0.000). Conclusions Hospital managers can adopt effective strategies to raise the level of nurses' leadership, which further increases the nurse physician cooperation level.

ObjectiveTo observe the effects of ultrafiltration extract from Angelica Sinensis Radix and Hedysari Radix on PC12 cell apoptosis induced by H2O2; To discuss its mechanism of action.Methods H2O2 was used in the incubation of PC12 cells to establish the oxidative damage nerve cell apoptosis model. The experiment was divided into normal control group, model group, and three different dosages (0.375, 0.75, 1.5 g/L) of ultra-filtration extracted from Angelica Sinensis Radix and Hedysari Radix after modeling for interference. Rate of cell apoptosis was detected by flow cytometry; mitochondrial membrane potential was measured by laser scanning confocal microscopy; the protein expressions of cleaved Caspase-3, Bax and Bcl-2 were detected by Western blot.Results Compared with the model group, rates of cell apoptosis in the different dosage groups decreased significantly; membrane potential of mitochondria increased; the protein expressions of cleaved Caspase-3 and Bax decreased; the expression of Bcl-2 increased; the ratio of Bcl-2 and Bax increased (P<0.05).Conclusion The ultra-filtration extracted from Angelica Sinensis Radix and Hedysari Radix can inhibit PC12 cell apoptosis induced by H2O2.

Objective:To investigate the expression of Survivin and programmed cell death 5(PDCD5)protein in mucoepidermoid carcinoma(MEC).Methods:Survivin and PDCD5 were detected by immunohistochemical staining in 20 cases of normal parotid tis-sue(group A),40 cases of pleomorphic adenoma(group B)and 45 cases of MEC tissues(group C).Results:The positive expres-sion ratio of Survivin in group A,B and C was 10.0%,27.5% and 55.6% respectively(χ2 =14.556,P <0.01),while that of PD-CD5 was 85%,65% and 33.3% respectively (χ2 =17.439,P <0.01).The expression of survivin or PDCD5 was related with dif-ferentiation,lymph node metastasis and TNM staging of MEC.Survivin and PDCD5 showed a negative correlation in MEC(χ2 =4.500,P =0.034,γs =-0.316).Conclusion:Survivin over-expression and PDCD5 down-expression may play a role in the de-velopment and progress of mucoepidermoid carcinoma.

Objective To explore the pain beliefs and perceptions held by patients with digestive system cancer.Methods 10 patients with digestive system cancer were interviewed for exploration of their pain beliefs and perceptions.The data were collected using in-depth interview and analyzed with category approach.Results The beliefs and perceptions held by patients with digestive system cancer were presented as:negative pain beliefs,negative emotional perceptions,view social support as important resources.Conclusions The pain beliefs and perceptions in digestive system cancer pain patients are negative.Clinical medical workers should pay attention to the role of beliefs in pain management,and help patients build positive pain beliefs through utilizing social support resources effectively.

Objective To investigate the characteristics of growth, accumulation of indole alkaloid, and the optical concentration of the nutrition components in the Catharanthus roseus mutant cells induced by the colchicine, looking forward to getting the ideal material producing alkaloids which was suited for industrialized cell cultivation. Methods Mutant cells cultivated on the MS medium were harvested according to experiment designs,and the fresh weight was got.The indole alkaloid as extracted by organic solvent. The ajmalicine and catharanthine extracted from the samples were analyzed by RP-HPLC. Results The growth rate was faster and the indole alkaloid accumulation was more when the mutant cells were cultivated generations 30 and the both were sharply decreased at generations 45, while the contents of medical compounds amounted to the peak at the generations 20. The content of medical components and alkaloid accumulation were evidently prompted by the tryptophan added in the media, especially at the concentration of Ca2+ 1 760 and Zn2+ 12.6 mg/L, respectively. Conclusion C.roseus mutant cells probably is an ideal material for industrialized cell cultivation.