Objective To investigate the characteristics of growth, accumulation of indole alkaloid, and the optical concentration of the nutrition components in the Catharanthus roseus mutant cells induced by the colchicine, looking forward to getting the ideal material producing alkaloids which was suited for industrialized cell cultivation. Methods Mutant cells cultivated on the MS medium were harvested according to experiment designs,and the fresh weight was got.The indole alkaloid as extracted by organic solvent. The ajmalicine and catharanthine extracted from the samples were analyzed by RP-HPLC. Results The growth rate was faster and the indole alkaloid accumulation was more when the mutant cells were cultivated generations 30 and the both were sharply decreased at generations 45, while the contents of medical compounds amounted to the peak at the generations 20. The content of medical components and alkaloid accumulation were evidently prompted by the tryptophan added in the media, especially at the concentration of Ca2+ 1 760 and Zn2+ 12.6 mg/L, respectively. Conclusion C.roseus mutant cells probably is an ideal material for industrialized cell cultivation.

Objective:Exploring the " bottle neck" factors in the scientific research management of recipient hospitals, making good use of counterpart support resources to help identifying appropriate, tailored strategies of scientific research management that might improve the research capacity of recipient hospitals.Methods:Data were collected according to questionnaire survey and on-site interview, ABC classification method were used to perform statistical analysis, and " bottleneck" factors that constraint the scientific research work of the recipient hospital were summarized.Results:" Insufficient scientific research skills and lacking of talents" and " lacking of scientific research environment and recognition" are the two most prominent factors that negatively affect the scientific research capacity building of the recipient hospitals, followed closely by " the out-dated scientific research policies and lacking support from the hospital leadership" , insufficient of research platform or resources including research funding, as well as other factors. Based on such findings, this article took the First People's Hospital of Kashgar (Guangdong counterpart support) as an example, and tried to discuss some corresponding measures on how to make good use of counterpart support resources.Conclusions:The ABC classification method were used to identify the main " bottleneck" factors, and a series of effective measures that help to making good use of counterpart support resources were explored. As a result, the efficiency of the scientific research management of the recipient hospitals, which in terms of management methods, management concepts and management models, were improved.

?? [Abstract]? Objective? To explore the correlation between walking gait characteristics and fall fear in hemiplegic patients with acute ischemic stroke. Methods? 189 patients with hemiplegia after acute ischemic stroke treated in the First Affiliated Hospital of Zhengzhou University from March 2016 to March 2018 were selected as the research subjects. The patients were investigated by General Data Questionnaire, Single Item Fall Fear Assessment and Modified Fall Efficacy Scale(MFES). The gait parameters of patients were measured by gait analyzer to detect and analyze the correlation between gait characteristics and fall fear in hemiplegic patients with acute ischemic stroke. Results? Among 189 patients with hemiplegia after acute ischemic stroke, 123 (65.08%) were fearful of falling and 66 (34.92%) were not fearful of falling. The average score of items in Single Item Fall Fear Assessment in the patients with fear of falling were (7.64±1.63) and those without fear of falling were (9.01±1.33), the difference between the two groups was statistically significan(t P＜0.05). The step speed, step frequency, step length and standing phase in the fear of falling group were lower than those in the non-fear of falling group, and the step time and swing phase were higher than those in the non-fear of falling group with statistical difference(P＜0.05). Pearson correlation analysis showed that the step speed, step frequency, step length and standing phase were positively correlated with the MFES score(P＜0.05), and the step time and swing phase were negatively correlated with the MFES score (P＜0.05). Conclusions? Most of the hemiplegic patients with acute stroke have the fear of falling, and those who are afraid of falling are more likely to have abnormal gait, which indicates that those who are afraid of falling are more likely to fall, and need the attention of family members and medical staff.

Objective:To explore the effects of postoperative rehabilitation nursing under the guidance of hierarchy of needs theory in patients with acute ischemic stroke.Methods:From February 2019 to February 2022, 120 patients with acute ischemic stroke who underwent interventional surgery in the Department of Neurology of the First Affiliated Hospital of Zhengzhou University were selected by convenient sampling. The patients were randomly divided into observation group and control group, with 60 cases each. The control group was given routine nursing after operation, and the observation group carried out postoperative rehabilitation nursing under the guidance of hierarchy of needs theory. The scores of the National Institute of Health Stroke Scale (NIHSS), Barthel Index, Pittsburgh Sleep Quality Index (PSQI), Hamilton Depression Rating Scale 17 (HAMD-17) and General Well-Being Schedule (GWB) were compared before and after the intervention.Results:After intervention, the NIHSS score of the observation group was lower than that of the control group, the Barthel index of the observation group was higher than that of the control group, the PSQI and HAMD-17 scores of the observation group were lower than those of the control group, and the GWB score of the observation group was higher than that of the control group, with statistically significant differences ( P<0.05) . Conclusions:The postoperative rehabilitation nursing under the guidance of hierarchy of needs theory is conducive to improving the neurological function, negative emotion and sleep quality of patients with acute ischemic stroke, and boosting their ability of daily life and well-being.

OBJECTIVETo propose a clinically practical and simple fiber tracking method for language pathways, and to explore its feasibility in preoperative planning for brain tumors adjacent to the language cortex.

METHODSDiffusion tensor imaging was examined in 18 healthy subjects and 13 patients with brain tumors adjacent to the language cortex between December 2013 and June 2014. The associated fibers of language pathways were reconstructed using a commercial software (Syngo workstation). Firstly, the feasibility of fiber tracking method for language pathways in healthy subjects were studied, and then its application was assessed in patients with brain tumors. The anatomic relationship between tumors and the associated fibers was analyzed.

RESULTSBy selecting appropriate regions of interest, the associated fibers in the dorsal pathways (superior longitudinal fasciculus/arcuate fasciculus, including both direct and indirect pathways) and ventral pathways (uncinate fasciculus, middle longitudinal fasciculus, inferior longitudinal fasciculus and inferiorfronto-occipital fasciculus) were reconstructed in all 18 healthy subjects. In patients with brain tumors, the relationship between the tumors and adjacent associated fibers were divided into two types: adjacent associated fibers could be displaced or separated, and involved the superior longitudinal fasciculus/arcuate fasciculus (n=6), middle longitudinal fasciculus (n=4), uncinate fasciculus (n=3), inferior longitudinal fasciculus (n=3) and inferiorfronto-occipital fasciculus (n=2); alternatively, the adjacent associated fibers were infiltrated or destroyed, and involved the inferiorfronto-occipital fasciculus (n=10), uncinate fasciculus (n=8), middle longitudinal fasciculus (n=5), inferior longitudinal fasciculus (n=4) and superior longitudinal fasciculus/arcuate fasciculus (n=3).

CONCLUSIONSThe associated fibers of language pathways could be visualized rapidly and in real-time by fiber tracking technology based on diffusion tensor imaging. This is feasible for preoperative planning regarding brain tumors adjacent to the language cortex.

Brain Neoplasms ; surgery ; Cerebral Cortex ; surgery ; Diffusion Tensor Imaging ; Humans ; Language ; Neural Pathways ; Postoperative Complications ; prevention & control

Objective:To construct a core competency evaluation index system for ophthalmic specialist nurses so as to provide theoretical reference for cultivation and evaluation of ophthalmic specialized nurses in China.Methods:A research group was set up in October 2020, and the core competency evaluation index system for ophthalmic specialist nurses was initially formed by means of literature review, semi-structured interview and group discussion. From December 2020 to January 2021, an online questionnaire was used to conduct Delphi letter consultation among 23 experts in related fields nationwide, and the items were screened and modified. The weight of indicators was determined by the proportional distribution method.Results:Two rounds of consultation were conducted. The positive coefficients of experts were respectively 91.30% and 100.00%, Kendall's concordance coefficients were respectively 0.172 and 0. 192 ( P<0.05) , and the expert authority coefficient was 0.90. Finally, an index system consisting of 5 first-level indicators, 12 second-level indicators and 58 third-level indicators was formed. Conclusions:The core competence evaluation index system of ophthalmic specialist nurses constructed in this study has a high degree of enthusiasm and authority of experts, which reflects the characteristics of the ophthalmic nursing specialty and provides theoretical reference for cultivation and ability evaluation of ophthalmic specialist nurses in China.

Patients with severe trauma require an extremely timely treatment and transfusion plays an irreplaceable role in the emergency treatment of such patients. An increasing number of evidence-based medicinal evidences and clinical practices suggest that patients with severe traumatic bleeding benefit from early transfusion of low-titer group O whole blood or hemostatic resuscitation with red blood cells, plasma and platelet of a balanced ratio. However, the current domestic mode of blood supply cannot fully meet the requirements of timely and effective blood transfusion for emergency treatment of patients with severe trauma in clinical practice. In order to solve the key problems in blood supply and blood transfusion strategies for emergency treatment of severe trauma, Branch of Clinical Transfusion Medicine of Chinese Medical Association, Group for Trauma Emergency Care and Multiple Injuries of Trauma Branch of Chinese Medical Association, Young Scholar Group of Disaster Medicine Branch of Chinese Medical Association organized domestic experts of blood transfusion medicine and trauma treatment to jointly formulate Chinese expert consensus on blood support mode and blood transfusion strategies for emergency treatment of severe trauma patients ( version 2024). Based on the evidence-based medical evidence and Delphi method of expert consultation and voting, 10 recommendations were put forward from two aspects of blood support mode and transfusion strategies, aiming to provide a reference for transfusion resuscitation in the emergency treatment of severe trauma and further improve the success rate of treatment of patients with severe trauma.