ObjectiveTo explore the relations between R peak time and cardiac function in pa- tients with mitral incompetence. Methods Left ventricular ejection fraction of 33 patients (subject group) less 50％ and 22 patients (control group) over 50％ was given ECG to determine R peak time. ResultsThe max average of R peak time was higher in subject group than in control group (P ＜ 0. 0001). ConclusionThe results indicate that R peak time has negative correlation to cardiac function.

To investigate whether accelerated blood clearance (ABC) phenomenon could be induced after repeated injection of mitoxantrone thermosensitive liposomes, LC-MS/MS and enzyme linked immunosorbent assay (ELISA) were used to measure the concentration of mitoxantrone and the anti-polyethylene glycol (PEG) IgM levels in rat plasma, separately. The drug was rapidly cleared away after the second administration. The anti-PEG IgM was detected after the first dose which was neutralized quickly after the second dose. It is proved that repeated administration of mitoxantrone thermosensitive liposomes in rat caused the ABC phenomenon.
Animals ; Antineoplastic Agents ; administration & dosage ; blood ; pharmacokinetics ; Chromatography, High Pressure Liquid ; Immunoglobulin M ; blood ; Liposomes ; administration & dosage ; blood ; pharmacokinetics ; Male ; Metabolic Clearance Rate ; Mitoxantrone ; administration & dosage ; blood ; pharmacokinetics ; Polyethylene Glycols ; administration & dosage ; chemistry ; pharmacokinetics ; Rats ; Rats, Wistar ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry

Objective To investigate the etiology,clinical features and outcome of hospitalized patients with bloodstream infections (BSIs) in a tertiary hospital.Methods Positive blood cultures were obtained from the microbiological laboratory in Fuxing Hospital,Capital Medical University from January 1,2012 to December 31,2012.BSIS events were identified and the epidemiology data were collected.Results A total of 149 patients and 154 BSIs events were confirmed by pathogenic and clinical evidence.The inpatients' BSIs rate was 0.8％ in our hospital in 2012.According to the disease entities of the first BSIs onset,15 patients (10.1％) were from surgical departments,83 patients (55.7％) from the medical departments,and 51 patients (34.2％) from ICU.Thirty-three patients (22.1％) were diagnosed as septic shock.Sixty-eight patients died during hospital stay.The in-hospital mortality rate was 45.6％.Among the 154 BSIs events,125 (81.2％) were nosocomial and 29 (18.8％) were community-acquired.A total of 188 strains were isolated from all BSIs,including 106 strains of (56.4％) gram-negative bacilli,67 (35.6％) strains of gram-positive bacteria,and 15 (8.0％) strains of fungi.One hundred and fifty-nine strains of bacteria (84.6％) were isolated from 125 events of hospital-acquired BSIs.Twenty-six strains of bacteria were from catheter related bloodstream infections (CRBSIs).In gram-negative BSIs,there were more enterobacteriaceae in community-acquired BSIs.More non-fermentative bacteria were found in hospitalacquired BSIs than in community-acquired ones.The distribution of gram-negative bacilli was quite different between surgical departments,non-surgical departments and ICU (P =0.049).Conclusions Pathogens of BSIs are quite different according to disease entities and where the patients are from.Local epidemiology of BSIs and distribution of related pathogens are helpful to physicians searching the optimal empirical antibiotics and improving the outcome.

Objective To investigate the efficacy of twice irradiating with focused ultrasound on recurrent and metastasized U14 cervical cancer implanted in the legs of mice. Methods Seventy-two mice with U14 cervical cancer cells implanted in their legs were divided into three groups randomly,with 24 rats in each group.One group received a single dose of focused ultrasound,while mice in the second group were irradiated twice and surgery resection was administered to the third group 7 or 8 days after the tumor was implanted.After 23 days post implantation of the tumor,local tumor recurrence and metastasis to the lungs and lymph nodes were evaluated. Results The inhibition rate after double irradiation was 61.70％ for local recurrence and 68.18％ for metastasis,significantly higher than in the other two groups. Conclusions Both single and double irradiation with focused ultrasound are effective for inhibiting local recurrence and metastasis,but double irradiation is more effective.

AIM: To examine T-lymphocyte rDNA transcription activity in peripheral blood of patients with gastrointestinal maliganant tumor and to clarify its clinical significance.METHODS: T-lymphocyte rDNA transcription activity in peripheral blood of 48 cases of patients with stomach-intestine tract malignant tumor were measured.RESULTS: Before surgery, the T-lymphocyte rDNA transcviption activity was obviously lower than that after surgery, also lower than that of the normal control( P

Objective To study the inhibitory effect of pterin acid (PTA) against ricin and recombinant ricin A chain protein. Methods Luciferase protein synthesis inhibition assay in a cell-free system and in vitro cytotoxicity experiments were performed to assess the biological activity of ricin and rRTA treated with PTA.Results The result showed that PTA could significantly inhibit the activity of ricin and rRTA in a dose-dependent manner.Conclusion PTA might be used as a small molecular probe to develop an evaluating system for ricin/RTA small molecular inhibitor in vitro. The cell-free system adopted in the current study could also serve as a necessary basis for screening some novel small molecular compounds against ricin and RTA in the future.

Brain Diseases ; diagnosis ; Child ; Child, Preschool ; Humans ; Magnetic Resonance Imaging ; methods ; Male ; Paragonimiasis ; diagnosis ; Tomography, X-Ray Computed ; methods

Objective To investigate the effect of sirolimus on tumor necrosis factor alpha (TNF-o)-induced lipid accumulation in HepG2 cells.Methods Palmitate acid (PA)-loaded HepG2 cells were divided into three groups:Control group(0.2％ BSA + 0.16 mmol · mL-1 PA + 25 ng· mL-1 TNF-o),model group(0.2％ BSA +0.16 mmol · mL-1 PA +25 ng · mL-1TNF-α),experimental group(0.2％ BSA + 0.16 mmol · mL-1 PA +25 ng · mL-1 TNF-α + 10 ng · mL-1 sirolimus).After 24 h treatment,lipid accumulation in the HepG2 cells was visualized using oil red O staining.The phosphorylation of mammalian target of rapamycin (mTOR)and its downstream translation regulator including P70 ribosomal protein S6 kinase (P70S6K) in HepG2 cells were detected by Western blotting.The mRNA expression of sterol regulatory element binding protein 1 (SREBP1),acetyl CoA carboxylase(ACC),fatty acid synthetase(FAS) were detected by real-time PCR.Results TNF-o-induced lipid accumulation was significantly inhibited by sirolimus in HepG2 cells.The TNF-α-induced phosphorylation of mTOR and its downstream translation regulator P70S6K were also reduced by sirolimus:the relative levels of p-mTOR protein in control group,model group and experimental group were 1.00 ± 0.20,3.11 ± 0.60,2.38 ± 0.50,respectively;the relative levels of p-P70S6K protein in that three groups were 1.00 ±0.30,3.67 ±0.60,1.62 ±0.50,respectively.The differences between model group and control group or experimental group and model group were statistically significant (all P ＜ 0.05).Additionally,the mRNA expression of SREBP1,ACC and FAS were downregulated by sirolimus:the mRNA levels of FAS in control group,model group andexperimental group were 1.04 ± 0.32,2.85 ±0.90,1.68 ± 0.38,respectively;the mRNA levels of ACC in that three groups were 1.04 ± 0.30,3.23-±1.33,2.07 ± 0.52,respectively;the mRNA levels of SREBP1 in that three groups were 1.01 ± 0.16,3.85 ± 1.30,2.82 ±0.57,respectively.The differences between model group and control group or experimental group and model group were statistically significant (all P ＜ 0.05).Conclusion Sirolimus ameolirates TNF-α-induced lipid accumulation in HepG2 cells through inhibiting the mTOR signaling pathway.

Triterpenes, which have large application potential in the treatment of cancer, are the main active components of genuine medicinal material Alisma orientale (Sam.) Juzep. Farnesyl pyrophosphate synthase (FPPS) is one of the important rate-limiting enzymes in the synthetic pathway of triterpenes. In this study the FPPS full length cDNA of the A. orientale, was cloned via homology-based cloning approach and rapid amplification of cDNA ends (RACE). The full length of the FPPS cDNA was 1 531 bp (accession no. HQ724508), which contained a full 1 032 bp ORF that encoded 343 amino acids. The deduced protein sequence exhibited five conserved motifs, two of which is riched of Asp (DDXXD). The result of real-time quantitative PCR (QRT-PCR) showed that FPPS gene was expressed in different organs of A. orientale. The expression increased from October to the first ten-day period of December, and then decreased. The FPPS gene expression was higher in leaves but lower in leafstalk, tuber and root. HPLC analysis of active components 23-acetyl-alismol B of A. orientale. during different periods indicated that its change trend should be consistent with FPPS gene expression. It can be primarily deduced that FPPS gene should be an important control point in the synthetic pathway of Alisma terpenes. This study may facilitate the quality of medicinal plants through gene engineering in the future.
Alisma ; enzymology ; genetics ; Amino Acid Sequence ; Base Sequence ; Cloning, Molecular ; Computational Biology ; Conserved Sequence ; DNA, Complementary ; genetics ; DNA, Plant ; genetics ; Gene Amplification ; Geranyltranstransferase ; genetics ; isolation & purification ; metabolism ; Molecular Sequence Data ; Phylogeny ; Plant Leaves ; enzymology ; genetics ; Plant Roots ; enzymology ; genetics ; Plants, Medicinal ; enzymology ; genetics ; RNA, Messenger ; metabolism ; Real-Time Polymerase Chain Reaction ; methods

Coptidis Rhizoma and Aconiti Kusnezoffii Radix represent hot Chinese medicine and cold Chinese medicine respectively. The purpose of this study is to observe the differentiation effect of Coptidis Rhizoma and Aconiti Kusnezoffii Radix on lewis lung cancer and compare effect of hot Chinese medicine and cold Chinese medicine on tumor progression. In this study, the rat serum containing Coptidis Rhizoma or Aconiti Kusnezoffii Radix was prepared to treat lewis lung cancer cells in vitro, and effects of the serum containing Coptidis Rhizoma or Aconiti Kusnezoffii Radix on cell differentiation, proliferation, adhesion, succinic dehydrogenase (SDH) activity and gap-junction intercellular communication (GJIC) were investigated. In vivo, the subcutaneous implant model and pulmonary metastasis model of lewis lung cancer were established. Tumor bearing mice were taken water decoction of coptis chinensis or aconite by intragastric administration bid for four weeks, and the influences of coptis chinensis and aconite on tumor progression were evaluated by body temperature, blood oxygen saturation, red cell ATPase, blood rheology, intratumor hypoxia, capillary permeability and GJIC. The results showed that the serum containing aconite could induce cell differentiation, inhibit cell proliferation and migration, promote SDH activity and GJIC in lewis lung cancer cells. The serum containing Coptidis Rhizoma increased cell adhesion and decreased SDH activity and GJIC without cell differentiation although it also suppressed cell proliferation. Aconiti Kusnezoffii Radix water decoction could keep body temperature, blood oxygen saturation, red cell ATPase and blood rheology, and improve intratumor hypoxia, capillary permeability and GJIC in tumor bearing mice, which led to slower tumor growth and less metastasis. Coptidis Rhizoma water decoction decreased body temperature, blood oxygen saturation, red cell ATPase, blood rheology and GJIC, and promoted intratumor hypoxia and capillary permeability, which resulted to more tumor metastasis although it also prevented tumor growth. These results suggested that the hot Chinese medicine could induce tumor cell differentiation and prevent tumor poison invagination, which is better for tumor treatment than cold Chinese medicine.
Aconitum ; chemistry ; Animals ; Antineoplastic Agents ; pharmacology ; Carcinoma, Lewis Lung ; pathology ; Cell Differentiation ; drug effects ; Cell Line, Tumor ; Curcuma ; chemistry ; Drugs, Chinese Herbal ; pharmacology ; Mice ; Neoplasm Metastasis ; Rats ; Xenograft Model Antitumor Assays