1.Changes of corneal endothelial cell after phacoemulsification for patients with different preoperative level of HbA1 c
International Eye Science 2015;(7):1158-1160
AlM: To observe the influence ofphacoemulsification on corneal endothelial cell in patients with the different level of HbA1c.METHODS:With case-control study, 164 eyes from 115 cataract patients were divided into four groups according to the preoperative HbA1c level. Control group A: 43 eyes from 30 cases without diabetes ( HbA1c≤6. 5%). Diabetes groups:group B was consisted of 38 eyes from 26 cases(HbA1c≤6. 5%), 40 eyes of 28 case was in group C (6. 5%
2.Clinical value analysis of atorvastatin in treatment of patients with arteriosclerotic cerebral infarction
Chinese Journal of Biochemical Pharmaceutics 2014;37(7):144-146
Objective To evaluate the clinical value analysis of atorvastatin in the treatment of patients with arteriosclerotic cerebral infarction. Methods 100 patients with atherosclerotic cerebral infarction from January 2012 to October 2013 in the first people's hospital of lianyungang were researched.They were divided randomly into observation and control groups.Observation group had 50 patients,they were treated by using of conventional treatment with atorvastatin therapy.Control group had 50 patients,using conventional therapy.Two groups'Clinical outcomes,inflammatory cytokines and lipids were compared.Results There was no significant difference in the control group and the observation group in the clinical data ;Total efficiency rate in the observation group and the control group was 90.00% and 70.00%,respectly.Total efficiency rate of observation group was higher than the control group,there was a significant difference (u=2.4696,P=0.0135 );before treatment,there was no significant difference of patients in the control group and the observation group in IL-6,hs-CRP,TG,TC,LDL-C and HDL-C;after treatment,IL-6 of observation group was lower than the control group,there was a significant difference (P<0.0001);hs-CRP of observation group was lower than the control group,there was a significant difference (P<0.0001);TG,TC,LDL-C of observation group was lower than the control group,there was a significant difference (P<0.05);HDL-C of observation group was higher than the control group,there was a significant difference (P<0.05).Conclusion The clinical value analysis of atorvastatin in the treatment of patients with arteriosclerotic cerebral infarction is well.It is worthy of promotion in clinic.
3.Changes and their relationship between cognitive function and event-related potentials in patients with carotid atherosclerosis
Journal of Clinical Neurology 2001;0(05):-
Objective To investigate the changes and their relationship between cognitive function and event-related potentials(ERP)in patients with carotid atherosclerosis.Methods 65 patients with varying degrees of carotid atherosclerosis were carried out Mini-Mental State Examination(MMSE)and Frederick ERP P300 investigation and correlation analysis was done,compared with the control group.Results Compared with the control group,account ability,short-term memory and graphics portrayed score decreased in mild and moderate carotid atherosclerosis group(all P
4.Effect of sulfonylurea compounds on expression of sulfonylurea receptor 2 in myocardium of diabetic Goto-Kakizaki rats
Academic Journal of Second Military Medical University 2000;0(07):-
0.05).Conclusion:The diabetes itself does not affect the sulfonylurea receptor(SUR2)expression in myocardial tissues.Sulfonylureas at treatment dosage have no effect on receptor expression of SUR2.
5.The effect of colchicine on the proliferation of human orbital fibroblast in thyroid associated ophthalmopathy in vitro
Yan, LIU ; Bei, XU ; Xue-liang, XU
Chinese Journal of Experimental Ophthalmology 2013;(7):630-635
Background The orbital fibroblasts (OFs) in thyroid associated ophthalmopathy (TAO) play important roles in the proliferative and inflammatory response.Seeking the drug which inhibit OFs growth is of a vital significance for the prevention and treatment of TAO.Research documented that colchicine has an anti-fibrosis effect.But its influence on OFs of TAO patient is few known.Objective This study was to investigate the effect of colchicine on growth and apoptosis of OFs in vitro.Methods The retroobital connective tissue was obtained form 3 TAO patients and cultured using explant method.OFs were passaged and identified by immunochemistry,and 3-8 genetaions of cells were used in the study.Colchicine at the concentrations of 1 × 10-8,1 × 10-7,1 × 10-6,1 × 10-5,1 ×10-4 mol/L was added into the RPMI 1640 with 10% fetal bovine serum(FBS) to incubated the cells for 24,48 and 72 hours respectively,and only RPMI 1640 was used to culture the cells as the control group.Cell counting kit-8 (CCK-8)was used to detect the absorbance value (A450) of OFs for the evaluatuion of OFs and the inhibitory rate of colchicine to OFs.The colchicine of 1 ×10-6,1 ×10-5,1 × 10-4 mol/L was added into the culture medium for 48 hours,and then the apoptotic rate of the cells and the cell percentage in various cellular cycle was assayed by flow cytometry(FCM).The expression of transforming growth factor-β (TGF-β)in the cells was detected by immunochemistry to assess the influence of colchicine on the serection of the cells.Results Cultured cells showed the spindle-like in shape and the cell number was significantly increased with the incubation time.After incubated with 1 × 10-4,1 × 10 5,1 × 10-6,1 ×10-7,1 × 10-8 mol/L colchicines,the A450 values were gradually reduced with the increase of the concentrations of colchicine(F ion =62.004,P<0.05),and significant differences were found between different contrations of colchicine groups(all P<0.05).Aslo,gradually declined A450 values of the cells were seen with the lapse of culture time among the groups(Ftime =459.582,P<0.05).The inbitory rate of colchicine to the cells was elevated with the increase of concentrations.The apoptotic rates of the cells were (1.73 ± 0.15) %,(21.04 ± 4.56) %,(31.84 ±6.21)%and(35.32±5.56)% in the control group and 1 × 10-6,1 × 10-5,1 × 10 4 mol/L colchicine groups respectively,with statistically significant difference among the 4 groups (F =83.905,P<0.05).With the increase of concentrations of colchicines,the cell percentage in G2 +M phase lessened gradually,showing significant difference among the control group and the 1 × 10-6,1 × 10-5,1 × 10-4 mol/L colchicine groups (F =20.443,P<0.05).The expression of the TGF-β in the cells was (97.60± 2.09) % in the control group,and that in the 1 × 10-4 mol/L colchicine group was (44.43 ± 3.96) %,presenting a significant difference between them (t =65.330,P < 0.05).Conclusions Colchicine can induce apoptosis of OFs and inhibit the prolilferation of OFs in a time-and dose-dependent manner probably by decreasing the TGF-β secretion
6.Gasless laparoscopic surgery using abdominal wall lifting technique in the field of general surgery
Binnan WANG ; Dongsheng XU ; Bei SUN
International Journal of Surgery 2009;36(6):401-404
Complications related to CO2 pneumoperitoneum in laparoscopy surgery have led to the devel-opment of gasless laparoscopic surgery using abdominal wall lifting technique.The technique uses facilitate laparoseopic surgery without conventional pneumoperitoneum and markly reduced in the risks of anesthesiolo-gy,especially in old patients with cardiopulmonary insufficiency.The review summarizes the application of gasless laparoscopie surgery using abdominal wall lifting technique in the field of general surgery.
7.Differential diagnosis of gallbladder adenomatous lesions with MRI
Bin SONG ; Jingjing XU ; Bei ZHANG
Journal of Practical Radiology 2015;(5):772-776
Objective To investigate the value of MR imaging in distinguishing adenomas from malignant tumors in gallbladder. Methods Clinical data and MR imaging features of 40 lesions in 37 patients with gallbladder adenomas (GA)confirmed by pathology were collected and reviewed retrospectively.Results The univariate analysis demonstrated that age (P =0.044),size (P =0.001), ratio of high-intensity signals on T2 WI (P =0.013),lobular margin (P <0.000 1),and tumor shapes (P =0.001)were statistically significantly different between adenomas and the malignancy.The Logistic regression analysis showed that only two parameters were significantly different for the prediction of malignant tumors of gallbladder,including lobular margin (P=0.002)and size (P=0.046).Further analysis with ROC curve showed that a threshold value of lesion diameter to distinguish the benign and malignancy could be at 1.59 cm. Conclusion Both lobular margin of GA and maximal diameter are independent factors in predicting the nature of GA,and the former exhibits more significant.Low ratio of high signal intensity in T2 WI and type circular shape are the contradictory factor for predic-ting adenocarcinoma of gallbladder.MRI is useful in the identification of patients with GA and gallbladder carcinoma developed from GA.
8.The change of plasma matrix metalloproteinases and its effects on post-stenting restenosis in the patients with acute coronary syndrome
Guanxue XU ; Ranzun ZHAO ; Bei SHI
Chinese Journal of Geriatrics 2009;28(10):808-811
Objective To investigate the change of matrix metalloproteinase 3(MMP-3), matrix metalloproteinase 9 (MMP-9) and tissue inhibitors of metalloproteinase 1 (TIMP 1) in arterial plasma and their effects on post-stenting restenosis in the patients with acute coronary syndrome(ACS). Methods MMP-3, MMP-9 and TIMP-1 in arterial plasma were detected by enzyme-linked immunosorbent assay (ELISA) in 132 patients with ACS. The 132 patients were divided into two groups: restenosis group (n = 21) and non-restenosis group (n = 111), while 50 persons were as a normal control group. Results The levels of MMP-3[(15.99±4.30) vs. (4.86±2.98) ng/L, P=0.022] and MMP-9 [( 1.41±3.06) vs. (3.79±1.46) ng/L, P=0.041] in arterial plasma were higher in ACS group than in the control group. And the level of TIMP-1 tended to increase. The levels of MMP-3 and MMP-9 in arterial plasma were higher after stenting than before stenting. It reached a peak in the first 48 hours, and was still significantly increased in one week. The level of TIMP-1 was similar, but it reached the peak slowly. Before stenting, the level of MMP-9 was higher in restenosis group than in non-restenosis group [( 17.94±6.44) vs. (9.93±2.19) ng/L, P =0.003], but there were no differences between the two groups in the levels of TIMP-1 and MMP-3. During follow-up, the levels of MMP-3[(21.66±2.72) vs. (14.27±1.28) ng/L, P=0.033] and MMP-9[(22.81±5.31) vs. (12.10±2.76) ng/L, P = 0.039] were higher in the restenosis group than in the non restenosis group, but there was no difference between the two groups in the level of TIMP-1. Conclusions The increase of MMP-3 and MMP-9 in arterial plasma may contribute to the pathophysiological progress after stenting in patients with ACS, which may be a prediction for restenosis.
9.Effects of sevoflurane preconditioning and postconditioning on activity of NF-κB during lung ischemia-reperfusion in rats
Guiping XU ; Bei LIU ; Ning DU
Chinese Journal of Anesthesiology 2014;34(10):1241-1243
Objective To evaluate the effects of sevoflurane preconditioning and postconditioning on the activity of NF-κB during lung ischemia-reperfusion (I/R) in rats.Methods Fifty-four adult male Sprague-Dawley rats,weighing 300-350 g,were randomly divided into 3 groups (n =18 each) using a random number table:sham operation group (S group),I/R group and sevoflurane preconditioning and postconditioning group (SPP group).Lung I/R was induced by clamping the left pulmonary hilum for 45 min followed by 120 min reperfusion in I/R and SP groups.The left pulmonary hilum was only isolated but not ligated in group S.In group SP,2.1% sevoflurane was inhaled for 30 min followed by 10 min washout,the left pulmonary hilum was then ligated for 45 min,and 2.1% sevoflurane was inhaled for 30 min again starting from onset of reperfusion.Six rats were chosen at 30,60 and 120 min of reperfusion and sacrificed in each group,lungs were removed for determination of wet/dry lung weight (W/D) ratio and content of tumor necrosis factor-α (TNF-α) (by ELISA) and expression of nuclear transcription factor κB (NF-κB) p65 of nuclear protein (by Western blot) and for microscopic examination of the pathologic changes of lungs (with light microscope).Results Compared with S group,W/D ratio,TNF-α content and nuclear protein NF-κB p65 expression in lung tissues were significantly increased at each time point of reperfusion in I/R and SPP groups.Compared with I/R group,W/D ratio,TNF-α content and nuclear protein NF-κB p65 expression in lung tissues were significantly increased at each time point of reperfusion,and the pathological changes of lungs were attenuated in SPP group.Conclusion Sevoflurane preconditioning combined with postconditioning can reduce the lung I/R injury in rats through inhibiting NF-κB activity in lung tissues.
10.Inhibition of LPS-induced expression of myeloid differentiation factor 88 by fenoterol is associated with its anti-inflammatory effect
The Journal of Practical Medicine 2014;(18):2890-2893
Objective To explore the molecular mechanism of inhibition of LPS-induced inflammation by fenoterol, a β2 adrenoceptor agonist in monocyte. Methods Concentrations of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α) and MCP-1 from cell supernatants from THP-1 cells and wild type or MyD88- / - mice peritoneal macrophages stimulated by LPS in the presence or absence of fenoterol were determined by use of an ELISA system. Expression of MyD88 (myeloid differentiation factor 88) stimulated by LPS in the presence or absence of fenoterol were determined by Western blot. Results Fenoterol inhibited LPS-induced activation of MyD88 and secretion of inflammatory cytokines (TNF-α, MCP-1, and IL-1β). The reaction of MyD88- / - mice peritoneal macrophages to LPS was much lower than that of the wild type mice peritoneal macrophages. Conclusions MyD88 plays an important role in inflammation induced by LPS. The inhibition of LPS-induced expression of MyD88 by fenoterol is associated with its anti-inflammatory effect.