Objective To evaluate the precision of GFR using Gates method and compared with the results from renal pathological changes. Methods Twenty-seven patients whose 99Tcm-DTPA renograms had no obvious uptake phase were enrolled in Group A, and 27 patients whose 99Tcm-DTPA renograms had obvious uptake phase were enrolled in Group B. The measurement of GFR by Gates method was compared to the creatinine clearance measured and predicted by Cockroft-Gault (C-G), modification of diet in renal disease (MDRD) and SCr level. Renal pathological changes in two groups were compared using Pearson correlation and t test analysis. Results In Group A, GFR determined by Gates method did not show correlation with that estimated by C-G or 1/SCr (r = 0. 357,0. 376, both P ＞0.05), but was significantly correlated with GFR estimated by MDRD(r = 0. 440, P ＜ 0.05). In Group B, GFR determined by Gates method showed significantly correlation among GFR estimated by MDRD, C-G, and 1/SCr (r =0. 471, 0. 527,0. 452, all P ＜ 0.05). Renal tubulointerstitial damage score in Group A was higher than that in Group B (7.15±2.32, 3.70±3.06, t=4.66, P ＜0.001). Conclusions GFR determined by Gates method is less precise when 99Tcm-DTPA renogram has no obvious uptake phase than that when 99Tcm-DTPA renogram has obvious uptake phase. Renal tubulointerstitial damage is a strong indicator of no obvious uptake phase in 99Tcm-DTPA renogram.

OBJECTIVETo explore the clinical effect of low dose pituitrin in children with septic shock.

METHODSA total of 48 pediatric cases with septic shock, in whom 6 hours, conventional treatment could not reverse shock from January 2008 to December 2010, were selected for this study. The patients were divided into two groups randomly (completely random design) (control group 24, remedial group 24). The conventional treatment included antibiotics/fluid resuscitation/correcting acid-base imbalance, glucocorticoid, organ (heart/lung) support, dopamine 1 - 15 µg/(kg·min) and norepinephrine 0.5 - 2 µg/(kg·min) pumped in continuously in the control group. In initial 6 hours the same treatment was given to the remedial group, while low dose pituitrin (0.01 - 0.03 U/min) was pumped additionally during the rest of time. The therapeutic effect on correcting shock was evaluated in both groups.

RESULTSThe total effective rate was 76.2% in the remedial group and 40.0% in the control group; the mortality was 33.3% and 60% respectively. The difference between both groups was significant (P = 0.025).

CONCLUSIONLow dose pituitrin could improve the clinical effect significantly in children with septic shock in whom 6 hours conventional treatment failed to correct shock, shorten the total periods of treatment, and decrease mortality.

Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Norepinephrine ; therapeutic use ; Pituitary Hormones, Posterior ; administration & dosage ; therapeutic use ; Shock, Septic ; drug therapy ; Treatment Outcome ; Vasoconstrictor Agents ; therapeutic use

BACKGROUNDRopivacaine and levobupivacaine have been introduced into obstetric analgesic practice with the proposed advantages of causing less motor block and toxicity compared with bupivacaine. However, it is still controversial whether both anesthetics are associated with any clinical benefit relative to bupivacaine for labor analgesia. This study aimed to compare the analgesic efficacy, motor block and side effects of bupivacaine, ropivacaine and levobupivacaine at lower concentrations for patient-controlled epidural labor analgesia.

METHODSFour hundred and fifty nulliparous parturients were enrolled in this randomized clinical trial. A concentration of 0.05%, 0.075%, 0.1%, 0.125% or 0.15% of either bupivacaine (Group B), ropivacaine (Group R) or levobupivacaine (Group L) with sufentanil 0.5 microg/ml was epidurally administered by patient-controlled analgesia mode. Effective analgesia was defined as a visual analogue scale score was

RESULTSThere were no significant differences among groups in the numbers of effective analgesia, pain scores, hourly local anesthetic amount used, sensory and motor blockade, labor duration and mode of delivery, side effects and maternal satisfaction (P>0.05). The relative median potency was bupivacaine/ropivacaine: 0.828 (0.602-1.091), bupivacaine/levobupivacaine: 0.845 (0.617-1.12), ropivacaine/levobupivacaine: 1.021 (0.774-1.354), respectively. However, a significantly less number of effective analgesia and higher hourly local anesthetic use were observed in the concentration of 0.05% than those of >or=0.1% within each group (P<0.05).

CONCLUSIONSUsing patient-controlled epidural analgesia, lower concentrations of bupivacaine, ropivacaine and levobupivacaine with sufentanil produce similar analgesia and motor block and safety for labor analgesia. The analgesic efficacy mainly depends on the concentration rather than the type of anesthetics.

Adult ; Amides ; therapeutic use ; Analgesia, Epidural ; methods ; Analgesia, Obstetrical ; methods ; Analgesia, Patient-Controlled ; methods ; Anesthetics, Local ; therapeutic use ; Bupivacaine ; analogs & derivatives ; therapeutic use ; Female ; Humans ; Labor Pain ; drug therapy ; Labor, Obstetric ; Pregnancy ; Sufentanil ; therapeutic use ; Young Adult

OBJECTIVETo investigate fluorescence in situ hybridization labeled with quantum dots (QDs) for the detection of human papillomavirus 16/18 (HPV16/18) infection in cervical carcinoma patients.

METHODSA total of 80 biopsy samples of squamous carcinoma of cervix were assayed for HPV 16/18 infection by using quantum dot labeled fluorescent in situ hybridization (QD-FISH) and chromogenic in situ hybridization (CISH) techniques, respectively. The results obtained by using two different methods were statistically analyzed.

RESULTSThe positive rate for HPV16/18 by QD-FISH was 88.8% (71/80), higher than that (80.0%) by CISH, however, the result was statistically not significant (P=0.127). The positive detection rates for HPV16/18 by using both methods increased coincidentally with raising of the tumor grading stage.

CONCLUSIONThe sensitivity and specificity of HPV infection detectable by QD-FISH is higher than that by the CISH technique.

Adult ; Carcinoma, Squamous Cell ; pathology ; virology ; Cervical Intraepithelial Neoplasia ; pathology ; virology ; Chromogenic Compounds ; Female ; Human papillomavirus 16 ; isolation & purification ; Human papillomavirus 18 ; isolation & purification ; Humans ; In Situ Hybridization, Fluorescence ; methods ; Middle Aged ; Papillomavirus Infections ; virology ; Quantum Dots ; Sensitivity and Specificity ; Uterine Cervical Neoplasms ; pathology ; virology

OBJECTIVE To study the differences of gastric receptivity and emptying function of functional dyspepsia(FD) patients with different syndromes by liquid nutrient load test combined with B ultrasound examination.METHODS Seventy four subjects were included,which included 14 healthy cases(HS group),60 FD patients(33 cases of spleen deficiency and qi stagnation,16 cases of spleen deficiency and dampness accumulation,11 cases of spleen yang deficiency).Liquid nutrient load-ing test was performed for all subjects,and the threshold and maximum intake were recorded combined with VAS visual score. Gastric emptying rate and half emptying time of patients with different syndromes were compared by ultrasonic measurement. RESULTS The maximum intakes of three syndromes in FD group were lower than that in the HS group(P<0.05~0.01), and the maximum intake of spleen deficiency and dampness accumulation syndrome was lower than those of spleen deficiency and qi stagnation syndrome and spleen yang syndrome(P<0.05).Compared three syndromes in FD group with HS group, distal gastric emptying rate of postprandial 90min of spleen yang deficiency syndrome was lower than that of HS group(P<0.05).distal gastric emptying time of spleen deficiency and dampness accumulation syndrome was longer than that of spleen deficiency and qi stagnation syndrome and spleen yang deficiency syndrome in HS group(P<0.05).CONCLUSIONS There is difference in gastrointestinal motility between FD patients with the same syndrome,and that of the patients with spleen defi-ciency and dampness accumulation syndrome decreased more significant,which provides theoretical basis for TCM syndrome differentiation and treatment.

To evaluate the effects of rhG-CSF and rhSCF on mobilization of the peripheral blood stem cells, 15 monkeys were divided into control, rhG-CSF 10 micro g/(kg x day) and rhG-CSF 10 micro g/(kg x day) + rhSCF 50 micro g/(kg x day) treated groups. Monkeys were administered with vehicle, rhG-CSF and rhG-CSF + rhSCF subcutaneously once daily for 14 days, respectively. The results showed that the highest counts of leukocyte of rhG-CSF treated group were 411% of baseline value on day 7 after administration, compared with that of rhG-CSF + rhSCF treated group which were 538% on day 9. The highest counts of leukocytes lasted for 3 days in combined treated group. CFU-GM from peripheral blood in the two groups were 8.37 and 11.75 times higher at 5 and 9 days respectively after the mobilization. It is concluded that rhG-CSF significantly increases the number of peripheral blood leukocytes and CFU-GM, and a better effect can be obtained by rhSCF + rhG-CSF combined administration.
Animals ; Drug Therapy, Combination ; Female ; Granulocyte Colony-Stimulating Factor ; administration & dosage ; pharmacology ; Hematopoietic Stem Cells ; drug effects ; Leukocyte Count ; Leukocytes ; drug effects ; Macaca mulatta ; Male ; Recombinant Proteins ; pharmacology ; Stem Cell Factor ; administration & dosage ; pharmacology

Adolescent ; Adult ; Aged ; Female ; Humans ; Kidney Failure, Chronic ; mortality ; Male ; Middle Aged ; Prognosis ; Renal Replacement Therapy ; Retrospective Studies ; Survival Rate ; Vasculitis ; complications ; mortality

Thirteen isolates of Class I Newcastle disease virus obtained from healthy poultry in China during 2008 were characterized genotypically in this study. All the isolates were proved to be lentogenic strains based on the deduced amino acid sequence of the Fusion protein gene. Molecular epidemiological analysis showed that 13 isolates could be subdivided into 2 distinct genotypes, 11 isolates belonged to genotype 2, and other 2 isolates belonged to genotype 3. Results indicated two genotypes of Class I Newcastle disease virus might widely exist in domestic poultry in China.
Animals ; Birds ; China ; epidemiology ; Genotype ; Humans ; Molecular Epidemiology ; methods ; Newcastle Disease ; epidemiology ; virology ; Newcastle disease virus ; classification ; genetics ; pathogenicity ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; Viral Fusion Proteins ; genetics

Objective To observe the clinical efficacy and safety of ozagrel sodium injection combined with mild hypothermia in the treatment of craniocerebral injury acute cerebral infarction.Methods Sixty-two patients of craniocerebral injury acute cerebral infarction were randomly divided into control group (n =31 cases) and treatment group (n =31 cases).Control group was given Xueshuantong injection 10 mL qd,intravenous drip.Treatment group was taken with ozagrel sodium 100 mL bid,intravenous drip,and mild hypothermia treatment.Two groups were treated for 14 d.The clinical efficacy,national institutes of health stroke scale (NIHSS) scores,Barthel index (BI),changes of hemodynamic indexes and adverse drug reactions were compared between two groups.Results After treatment,the total effective rates in treatment and control groups were 90.32％ (28 cases/31 cases) and 61.29％ (19 cases/ 31 cases) with significant difference (P ＜0.05).After treatment,the main indexes in treatment and control groups were compared:NIHSS scores were (8.24 ± 0.76),(15.42 ± 1.65) points;BI were (75.23 ± 3.48),(52.32 ± 3.33) points;whole blood high shear viscosity respectively were (3.46 ±0.21),(4.63 ±0.26) mPa · s;whole blood low shear viscosity were (7.69 ±0.32),(8.38 ±0.31) mPa · s;plasma viscosity were (1.24 ±0.11),(1.76 ±0.16) mPa · s;fibrinogen were (3.07 ±0.41),(4.16 ±0.18)g · L-1,there were statistically significant differences (all P ＜0.05).No adverse drug reactions were observed during the treatment in two groups.Conclusion Ozagrel sodium injection combined with mild hypothermia has a definitive clinical efficacy and safety in the treatment of craniocerebral injury acute cerebral infarction,which can improve the patient's hemodynamic parameters,neurological function and activities of daily living.