1.Effect of Green Tea Polyphenols on N-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Induced Dopaminergic Neurodegeneration in Mice
Chinese Journal of Rehabilitation Theory and Practice 2008;14(3):239-240
Objective To investigate the protective effects of green tea polyphenols(GTPs)on dopamine neuron loss in substantia nigra concomitant with a depletion of dopamine in corpus striatum induced by the N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)in mice as a model of Parkinson disease.Methods Male C57BL/6 mice were intraperitoneally injected with saline + saline(group A),saline + GTPs(group B),saline+MPTP(group C)and GTP+MPTP(group D)at 2-hour intervals for a total of 3 doses for MPTP and 5 doses for GTPs(10 or 50 mg/kg delivered).The animals were sacrificed 7 d after the last injection.Levels of dopamine and its metabolites in the corpus striatum were measured with high-performance liquid chromatography with an electrochemical detector(HPLC-ECD).Results Level of dopamine and its metabolites in the corpus striatum in group C decreased significantly compared with group A or B;however,those in group D(both 10 and 50 mg/kg)prevented these effects.Conclusion GTPs can protect the dopamine neurons from loss in MPTP-induced mice.
2.The effects of high-fat diet during pregnancy and lactation on the behaviors and learning memory in adult male offspring
Yuanshao LIN ; Bei SHAO ; Yulei ZHOU
Chinese Journal of Behavioral Medicine and Brain Science 2015;24(2):101-104
Objective To investigate the effects of high-fat feeding during pregnancy and lactation on the behaviors and learning memory in adult male offspring,as well as explore its possible mechanisms.Methods Female SD rats were exposed to either high fat diet (HF) or normal diet (ND) during pregnancy and lactation period.From weaning,all male offspring were fed with ND until 120-day.The offspring whose mothers were fed with HF or ND received 14 d chronic unpredictable mild stress (CUMS) or normal circumstance,and being divided into ND group,ND+CUMS group,HF group and HF+CUMS group(9 rats in each group).The Open-field test,sucrose preference test and forced swimming test were used to evaluate the depressive-like behaviors,and Morris water maze test was employed to assess the learning and memory ability.Moreover,blood samples were taken via chronically implanted cardiac catheters for measurement of adrenocorticotropic hormone (ACTH) and corticosterone (CORT) levels in another set of DN group and HF group with normal circumstance during baseline and restraint stress (1 h).Results (1) Compared with the ND group,the sucrose preference in the HF group were decreased (P<0.05).Compared with ND group,ND +CUMS group showed more serious depressive-like behavior.Vertical score in open-field test (11.36±8.25) and sucrose preference(0.63±0.04) of HF+CUMS group,were lower than those of the ND+CUMS group ((17.12±7.54),(0.73±0.05),respectively).The immobility time in forced swimming test of HF+CUMS group ((33.16±6.35)s) were longer than that of ND+CUMS group ((25.74±7.31) s).(2) In Morris water maze test,the crossing platform times of the HF group was less than those of the HF group.Compared with ND+CUMS group,the target quadrant time,effective area residence time and crossing platform times of HF+CUMS group were decreased significantly (P<0.05).(3)There was no differences in basal ACTH and CORT between ND group and HF group (P>0.05).Both ND group and HF group exhibited significantly elevated levels of plasma ACHT and CORT during restraint,but without significant difference between these two groups (P> 0.05).Within 3 h after restraint,the HF group showed significant increase of ACTH and CORT compared with ND group(P<0.05).Conclusion As one kind of distress in early life,high-fat geeding through pregnancy and lactation increase the susceptibility and severity of depressive-like behaviors in adult offspring,as well as reducing the learning and memory ability,and the activity of hypothalamic-pituitary-adrenal axis activity during stress may contribute to the changes.
3.Influence of sequential enteral nutrition on nutritional status and prognosis in patients with acute cerebral infarction
Beilei ZHU ; Bei SHAO ; Zhen WANG
Journal of Clinical Neurology 2001;0(05):-
Objective To explore the influence of the sequential enteral nutrition(SEN) on nutritional status and prognosis in patients with acute cerebral infarction(ACI).Methods 80 ACI patients complicated with dysphagia were divided into SEN group and control group(40 cases in each group).In the first three days patients in SEN group were treated with nasal feeding short-peptide enteral nutrition[84~126(kJ/kg.d) ],than,which would be replaced with nasal feeding integral-protein enteral nutrition from the forth day.Patients in the control group were provided with ordinary fluid food by nasal feeding.Nutrition support for both groups was at last more than 10 d.In the 1 d,7 d and 14 d after treatment,data of both groups concerned with triceps skin fold(TSF),mean upper arm muscle circumference of triceps(MAMC),serum albumen(Alb),serum prealbum(PAB),heamoglobin(Hb),incidence of infectious complications,National Institutes of Health Stroke Scale(NIHSS) score and Barthel index(BI) were examined.Results Nutritional indexes(TSF,MAMC,Alb,PAB,Hb) in the SEN group were significantly higher than those in the control group(P
4.Correlation between variants of leukotriene A4 hydrolase gene and risk of ischemic stroke in population of southern Zhejiang Province
Kaikai HU ; Lifen CHI ; Xingyang YI ; Bei SHAO
Chinese Journal of Neurology 2015;48(5):368-372
Objective To investigate the correlation between two single nucleotide polymorphisms of the leukotriene A4 hydrolase (LTA4H) gene (rs2660845 and rs2540493) and risk of ischemic stroke in population of southern Zhejiang Province.Methods A total of 300 ischemic stroke patients and 300 healthy controls,recruited from the Department of Neurology,Third Affiliated Hospital of Wenzhou Medical University between September 2010 and June 2013,were enrolled in this study.Two single nucleotide polymorphisms of the LTA4H gene (rs2660845 and rs2540493) were analyzed by polymerase chain reaction and matrix-assisted laser desorption/ionization time of flight,respectively.Sixty-seven patients and thirty controls were randomly selected (complete randomization) and detected the serum leukotriene B4 (LTB4)concentration by ELISA method.Results There was no evidence of association between the two variants of LTA4H gene and the risk of ischemic stroke or its TOAST (Trial of Org 10 172 in acute stroke treatment)subtypes (P > 0.05).Analysis of LTB4 levels revealed that there was no statistically significant difference in serum LTB4 concentration between patients (n =67) and controls (n =30; 0.991 ± 0.305 vs 1.035 ± 0.498 ; P =0.692),and no statistically significant difference in LTB4 concentration was found among the three genotypes of rs2660845 as well (AG genotype vs AA genotype vs GG genotype:0.938 ± 0.269 vs 1.038 ± 0.268 vs 1.043 ± 0.383 ; P =0.401).Conclusion The present study suggests that there is no association between the two polymorphisms in the LTA4H gene and risk of ischemic stroke in population of southern Zhejiang Province.
5.Recent progress of migraine and pregnancy
Liuqing WANG ; Xuran XU ; Shoucheng ZHANG ; Hong WANG ; Bei SHAO
Clinical Medicine of China 2017;33(6):551-554
As a kind of nervous system disease,migraine is more common in female,and has the clinical characteristics of repeated attacks,it is of great significance with standardized treatment in the control of the attacks.Yong female patients with migraine during pregnancy and lactation stage will face lots of special problems because they must first consider the impact of treatment on the mother and fetus.Generally,non-drug therapy is recommended as a first-line treatment,if it is not sufficiently effective,paracetamol is recommended during the pregnancy or sporadic use of sumatriptan,NSAIDs is not recommended during the first or third trimester of pregnancy.Preventive therapy should only be considered in the most severe cases.This review summarized recent documents of the safety of the most used antimigraine medications during pregnancy and breastfeeding,in order to provide treatment recommendations in clinical practice.
6.Cinepazide Maleate Combined with Venlafaxine for Post-stroke Depression
Huanjie HUANG ; Bei SHAO ; Weiyong YIN ; Dalou HE
Chinese Journal of Rehabilitation Theory and Practice 2011;17(4):368-369
ObjectiveTo investigate the effect of cinepazide maleate combined with venlafaxine on post-stroke depression.Methods60 stroke patients with post-stroke depression were divided into control group (n=30) and observation group (n=30). On the basis treatment, the control group was given venlafaxine, the observation group was given venlafaxine and maleate cinepazide injection. The course was 4 weeks. The two groups were assessed with Hamilton Depression Scale(HAMD) and modified Barthel Index(MBI) before and after treatment.ResultsThe observation group improved better than the control group (P<0 05) even in HAMD and MBI 4 weeks after treatment(P<0.01).ConclusionCinepazide maleate combined with venlafaxine may be more effective on post-stroke depression to improve depressive symptoms, and the activities of daily living.
7.Clinical and Imaging Features of Hypertrophic Olivary Degeneration Secondary to Brainstem Hemorrhage: 1 Case Report
Dalou HE ; Bei SHAO ; Anbin BO ; Huanjie HUANG
Chinese Journal of Rehabilitation Theory and Practice 2013;19(12):1191-1193
Objective To investigate the clinical and imaging features of hypertrophic olivary degeneration (HOD) secondary to brainstem hemorrhage. Methods The clinical data of one patient with HOD secondary to brainstem hemorrhage was retrospectively analyzed. Results The patient was hospitalized with paroxysmal and body involuntary jitter and other extrapyramidal symptoms. After admission, MRI scan showed bilateral inferior olive nucleus of medulla oblongata were localized hypertrophy. Conclusion The main clinical manifestation of HOD secondary to brainstem hemorrhage is extrapyramidal symptom. The imaging features are abnormal signals and localized hypertrophy at inferior olive nucleus.
8.Role of extracellular signal-related kinase 1/2 signal transduction pathway at supraspinal level in maintenance of neuropathic pain in mice
Bei MIAO ; Yue YIN ; Tiantian ZHOU ; Cuijie SHAO ; Junli CAO ; Qiuping CHEN
Chinese Journal of Anesthesiology 2013;33(8):924-927
Objective To evaluate the role of extracellular signal-related kinase (ERK) 1/2 signal transduction pathway at the supraspinal level in maintenance of neuropathic pain in mice.Methods Sixty-four Kunming mice,aged 2 months,weighing 18-20 g,were randomly divided into 4 groups (n =16 each):sham operation group (group S),chronic constrictive injury (CCI) group; CCI + U0126 (MEK inhibitor) group; CCI + dimethyl sulfoxide (DMSO) group.Neuropathic pain was induced by CCI.The sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1mmintervals with 4-0 silk thread in CCI,CCI + U0126 and CCI +DMSO groups.On 5 days after CCI,5 μg U0126 (in 5 μl of 5% DMSO) and 5% DMSO 5 μl were injected into the lateral cerebral ventricle over 10 s in CCI + U0126 and CCI + DMSO groups,respectively,and the time of.needle retaining was 20 s.Paw withdrawal threshold to mechanical stimulation with yon Frey filament (MWT) and paw withdrawal latency to thermal stimulation (TWL) were measured before operation (baseline),before intracerebroventricular injection (T1),and at 30 min and 2,6,12 and 24h after intracerebroventricular injection (T2-6).Resuits Compared with group S,MWT was significantly decreased and TWL was shortened at T1-6 in CCI and CCI +DMSO groups,and at T1 in CCI + U0126 group (P < 0.05),while no significant change in MWT and TWL was found at T2-6 in group CCI + U0126 (P > 0.05).Compared with group CCI,MWT was significantly increased and TWL was prolonged at T2-6 in group CCI + U0126 (P < 0.05),while no significant change in MWT and TWL was found in group CCI + DMSO (P > 0.05).Conclusion ERK1/2 signaling transduction pathway at the supraspinal level is involved in maintenance of neuropathic pain in mice.
9.Application of bulbocavernosus reflex in differential diagnosis of patients with Parkinson's disease and multiple system atrophy
Huijing LI ; Xiaoting NIU ; Xun WANG ; Jie PAN ; Peiqi NI ; Bei SHAO
Chinese Journal of Neurology 2015;48(3):203-206
Objective To assess the value of bulbocavernosus reflex (BCR) in the differential diagnosis of Parkinson' s disease (PD) and multiple system atrophy (MSA).Methods BCR was compared in 55 patients with PD,20 patients with MSA,and 50 healthy controls,who were enrolled from the Department of Neurology,the First Affiliated Hospital of Wenzhou Medical University from May 2013 to April 2014.The clinical features of autonomic nerves dysfunction were also recorded.Results Among all related autonomic symptoms,the occurrence rate of constipation,urinary urgency and frequency in patients with MSA was higher than those with PD.The elicit percent of BCR in patients with PD was 93%,higher than those with MSA (70%).The average latency of BCR in patients with MSA was longer than those with PD (tmale left =16.275,tmale right =14.269,tfemale left =5.954,tfemmale right =5.905,all P < 0.05).The degree of BCR amplitude decreasing in three groups was MSA > PD > healthy controls.There was statistically significant difference among three groups (Fmale left =75.73,Fmale right =73.13,Ffemale left =72.70,Ffemale right =59.44,all P < 0.05).The area under receiver operating characteristic curve (ROC) in differential diagnosis of PD and MSA of the average latency of BCR in male and female was 0.947 and 0.948.The area under ROC curve in differential diagnosis of PD and MSA of the average amplitude of BCR in male and female was 0.886 and 0.920.The ROC curve showed the average latency of BCR in male of 44.80 ms with a sensitivity of 95% and a specificity of 84%,and in female of 61.35 ms with a sensitivity of 86% and a specificity of 88% ; the average amplitude of BCR in male of 0.37 mV with a sensitivity of 96% and a specificity of 68%,and in female of 0.36 mV with a sensitivity of 98% and a specificity of 76%,which were critical cutoff values in differential diagnosis of PD and MSA with the best sensitivity and specificity.Conclusion The latency and amplitude of BCR test helps to increase the accuracy in the differential diagnosis of PD and MSA.
10.Molecular mechanisms of antianxiety drugs on rats with restraint stress
Huanjie HUANG ; Bei SHAO ; Rongyuan ZHENG ; Jianmin LI ; Zongmin WANG ; Zhiqin YAN ; Liwei XIE ;
Chinese Journal of Clinical Pharmacology and Therapeutics 2004;0(10):-
AIM: To investigate the molecular mechanisms of antianxiety drugs on the rats with restraint stress. METHODS: The rat stress model was made by restraint stress. The behaviors of rats were tested in open field conditions, and the expression of c fos positive cells was detected by S P immunohistochemical assay in hypothalamus. RESULTS: The crossing scores, the rearing scores and the expression of c fos positive cells increased more significantly in the other groups than that in the control group, but decreased in the paroxetine group. The paroxetine inhibited the behaviors and the expression of c fos positive cells in the hypothalamic paraventricular nucleus (PVN) of rats after immobilization stress. CONCLUSION: The effects of paroxetine on the anxiety disorders in rats may be related to the downregulation of the expression of the c fos in the hypothalamic paraventricular nucleus (PVN).