1.The clinical significance of combined detection of combined detection of serum sTWEAK and cystatin C in early diabetic nephropathy
Bei CHAO ; Weifeng SHI ; Zhijian ZHANG
International Journal of Laboratory Medicine 2015;(8):1082-1083
Objective To investigate the clinical significance of combined detection of serum sTWEAK and cystatin C in early di‐abetic nephropathy (DN) .Methods 35 patients with DN(DN group) ,56 patients with diabetes mellitus (DM ,DM group) and 40 healthy cases conducted physical examination (healthy control group) were selected and serum levels of sTWEAK and cystatin C were detected .And levels of glycosylated hemoglobin(HbA1c) and qualitative urine protein test were conducted in DM group .Re‐sults The serum sTWEAK level in DN group was lower than that in DM group ,and the serum cystatin C level was higher than that in DM group(P<0 .05) .The serum levels of sTWEAK in DM group and DM patients with negative qualitative urine protein were lower than that in healthy control group ,while the serum cystatin C levels were higher than that of control group (P<0 .05) . Compared with DM patients with HbA1C< 10% ,the serum cystatin C level was higher and serum sTWEAK level was lower of those with HbA1c>10% (P<0 .05) .Conclusion The combined detection of serum sTWEAK and cystatin C could be a sensitive index for early diagnosis of DN ,with important significance in the occurrence and development of DN .
2.Short-term intensive insulin therapy in newly diagnosed type 2 diabetic patients
Qian WANG ; Jiaqing FENG ; Bei SHEN ; Chao LIU
Journal of Medical Postgraduates 2003;0(10):-
Objective:To investigate the effect of short-term intensive treatment with insulin on metabolic control,?-cell function and insulin resistance in newly diagnosed type 2 diabetic patients. Methods:Thirty-eight newly diagnosed type 2 diabetic patients were randomly divided into two groups and treated with continuous subcutaneous insulin infusion(CSII) or multiple daily insulin injection(MDI) for 1 month.Fasting blood glucose(FBG) and lipid profiles were measured,and oral glucose tolerance test(OGTT)was performed before and after the treatment.The second phase insulin and C peptide secretions were measured by calculating area under curve(AUC) in OGTT.HOMA model was used to evaluate ?-cell function((HOMA-?)) and insulin resistance. Results:Excellent control of FBG was achieved after 1 month intensive treatment.(HOMA-?) was improved significantly. Conclusion:The excellent metabolic control and ?-cell function improvement can be achieved by short-term intensive insulin therapies like CSII or MDI.
3.The correction of lingually inclined mandibular second molars
Min BEI ; Huang LI ; Qiaoling MA ; Lei HAN ; Chao LIU
Journal of Practical Stomatology 2016;32(2):283-285
6 cases of lingually inclined mandibular second molar were treated by ring uprightor,the malposition was corrected in all the ca-ses in 3 months on average.
5.Clear cell meningioma: report of a case.
Zhi-yi ZHOU ; Rong-chao SUN ; Shu-dong YANG ; Jia-bei LIANG ; Jun RUI
Chinese Journal of Pathology 2009;38(8):562-563
Diagnosis, Differential
;
Ependymoma
;
metabolism
;
pathology
;
Female
;
Hemangioblastoma
;
metabolism
;
pathology
;
Humans
;
Ki-67 Antigen
;
metabolism
;
Meningeal Neoplasms
;
metabolism
;
pathology
;
surgery
;
Meningioma
;
metabolism
;
pathology
;
surgery
;
Middle Aged
;
Mucin-1
;
metabolism
;
Neoplasm Recurrence, Local
;
Vimentin
;
metabolism
6.Surveillance of bacterial resistance from a hospital in Chongqing from 2004 to 2005
Chao ZHUO ; Bei JIA ; Wen-Xiang HUANG ; Xing-Ping ZHENG ; Chong-Zhi LI ;
Chinese Journal of Infection and Chemotherapy 2007;0(05):-
Objective To investigate the antimicrobial resistance of clinical isolates from a hospital in Chongqing during one year according to CHINET project.Methods Disc diffusion test (K-B method) was employed to study the antimicrobial resistance. WHONET5 was used for data analysis.Results In one year period from 2004 to 2005,690 non-duplicate isolates were collect- ed.Enterobacter isolates showed the lowest resistance rate to imipenem and meropenem.About 37.5% of E.coli and 31.4% of K.pneumoniae isolates produced ESBLs,respectively.All ESBLs-producing strains were susceptible to imipenem and mer- openem.About 37.2%,39.4% and 48.9% of P.aeruginosa isolates were resistant to imipenem,meropenem and ceftazidime, respectively.Pandrug-resistant (PDR) P.aeruginosa was isolated from our hospital.All strains of A.baumannii were sus- ceptible to imipenem and meropenem.About 37.7% of A.baumannii were resistant to cefoperazone-sulbactam.Twenty-nine strains showed the same resistant pattern among non-susceptible strains of A.baumannii,mainly derived from 2 clones by PFGE analysis.Conclusions The surveillance results suggest that prevalent strain resistant to cefoperazone-sulbactam may pres- ent in some ICUs.Resistance rate to cefoperazone-sulbactam increased significantly.
7.Not Available.
Chao ying FENG ; Meng HE ; Jun yi LIN ; Yi wen SHEN ; Bei xu LI
Journal of Forensic Medicine 2021;37(5):745-748
8.Expression of P504S,CK34?E12,p63 and PSA immunohistochemistry in pathological diagnosis of prostatic adenoearcinoma
Rong-Chao SUN ; Li-Hua ZHANG ; Shu-Dong YANG ; Ying CHEN ; Guang-Bin WU ; Jia-Bei LIANG
Chinese Journal of Urology 2000;0(12):-
Objective To investigate the utility of P504S(?-methylacyl-COA racemase), CK34?E12,p63 and PSA immunohistochemistry in the pathological diagnosis of prostatic adenocarcinoma (PAC).Methods The specimens of 46 cases of PAC,8 cases of prostatic high-grade intraepithelial neo- plasia (HGP1N) and 35 cases of benign prostatic hyperplasia (BPH) tissues were immunohistochemically stained with P504S,CK3413E12,p63 and PSA antibody,respectively.Results Of the 46 PAC cases,42 (91.3%) cases showed positive for P504S,including 25 cases (54.3%) who showed strongly and diffusely positive (+++) for cytoplasmic staining.In 7 (87.5%) of the 8 HGPIN cases,the specimens were also positive for P504S,and only 1(2.9%)of the 35 BPH cases showed focally weakly positive (+) for P504S.All the 8 HGPIN cases (100%) and 33 (94.3%) of the 35 BPH cases showed positive for CK34?E12 and p63,while all the 46 PAC cases showed negative for CK34?E12 and p63.In 44 (95.7%) of the 46 PAC cases,the specimens were positive staining for PSA.Conclusions P504S has high sensitivity and good specificity in the diagnosis of PAC.P504S staining in combination with HE,CK34?E12,p63 and PSA staining can improve the accurate diagnosis of PAC.
9.The impact on perioperative patients with non-small cell lung cancer by neoadjuvant chemotherapy.
Jia'an DING ; Xing'an WANG ; Bei YANG ; Chao LI ; Jun WANG
Chinese Journal of Lung Cancer 2003;6(6):488-491
BACKGROUNDTo investigate whether neoadjuvant chemotherapy (MVP) could influence the safety of perioperative patients with non-small cell lung cancer (NSCLC).
METHODSThe regimen of chemotherapy was MVP (mitomycin+vindesine+cisplatin) for all patients. The patients undergoing 2 cycles of neoadjuvant chemotherapy, radical resection and 2 cycles of postoperative chemotherapy were compared with those undergoing similar resections and 4 cycles of similar postoperative chemotherapy.
RESULTSOf the 107 eligible patients, 66 patients were in the neoadjuvant-chemotherapy group and 41 in control group. There was no statistical difference between these two groups in the distributions of gender, age, tumor staging and pathology. The neoadjuvant-chemotherapy group had longer operative duration (P=0.262), more operative blood loss (P=0.704), more amount of operative transfusion (P=0.811) and total amount of perioperative transfusion (P=0.074), and less amount of post-operative drainage (P=0.061) than those of the control group, but no statistical difference was found among them. No statistical difference was detected between two groups in the mortality (P=0.674) and the morbidity such as arrhythmia (P=0.608), bronchial parietal fistula (P= 0.378 ), pneumonia (P=0.622) and respiratory failure (P=0.285).
CONCLUSIONSNeoadjuvant chemotherapy does not exert significant influence on the safety of perioperative patients with NSCLC.
10.N6-Methyladenosine Methyltransferase METTL3 Alleviates Diabetes-Induced Testicular Damage through Modulating TUG1/Clusterin Axis
Yuan TIAN ; Yue-Hai XIAO ; Chao SUN ; Bei LIU ; Fa SUN
Diabetes & Metabolism Journal 2023;47(2):287-300
Background:
The present study investigated the regulatory effects of N6-methyladenosine (m6A) methyltransferase like-3 (METTL3) in diabetes-induced testicular damage.
Methods:
In vivo diabetic mice and high glucose (HG) treated GC-1 spg cells were established. The mRNA and protein expressions were determined by real-time quantitative polymerase chain reaction, Western blot, immunofluorescence and immunohistochemistry staining. Levels of testosterone, blood glucose, cell viability, and apoptosis were detected by enzyme-linked immunosorbent assay, MTT, and flow cytometry, respectively. Molecular interactions were verified by RNA immunoprecipitation and RNA pull-down assay. Histopathological staining was performed to evaluate testicular injury.
Results:
METTL3 and long non-coding RNA taurine up-regulated 1 (lncRNA TUG1) were downregulated in testicular tissues of diabetic mice and HG-treated GC-1 spg cells. METTL3 overexpression could reduce the blood glucose level, oxidative stress and testicular damage but enhance testosterone secretion in diabetic mouse model and HG-stimulated GC-1 spg cells. Mechanically, METTL3-mediated m6A methylation enhanced the stability of TUG1, then stabilizing the clusterin mRNA via recruiting serine and arginine rich splicing factor 1. Moreover, inhibition of TUG1/clusterin signaling markedly reversed the protective impacts of METTL3 overexpression on HG-stimulated GC-1 spg cells.
Conclusion
This study demonstrated that METTL3 ameliorated diabetes-induced testicular damage by upregulating the TUG1/clusterin signaling. These data further elucidate the potential regulatory mechanisms of m6A modification on diabetes-induced testicular injury.