Objective To study the integration of traditional medical journals and new media in the Internet +era. Methods The papers published in journals were integrated and a characteristic single disease misdiagnosis database was developed for providing precise and accurate information by making full use of the contributing, revie-wing, editing and proofreading platform into which the short message service, alipay or third party payment service and WeChat service were linked. Results The platform makes convenient for the authors, editors and reviewers of manuscripts, the database contributes to the deep development of journal contents and activates the journal vitality. Conclusion The traditional media can improve the work efficiency, integrate the journal contents and provide the characteristic service by making full use of modern technologies, thus contributing to the further development of journals.

Objective:To observe expressions of vascular endothelial growth factor (VEGF ) and placental growth factor (PLGF) in patients with hypertensive disorder complicating pregnancy (HDCP) during early and mid pregnancy ,and pro‐vide basis for diagnosing HDCP .Methods:A total of 46 normal pregnant women (normal pregnancy group ) and 39 HDCP patients (HDCP group) were enrolled .The HDCP group was further divided into gestational hypertension group (n=26) , mild preeclampsia group (n=8) and severe preeclampsia group (n=5) .Enzyme linked immunosorbent assay (ELISA) was used to measure serum VEGF and PLGF levels during early ,mid and late pregnancy ,and those were compared among a‐bove groups .Results:Compared with normal pregnancy group ,there were significant reductions in serum levels of VEGF [ (187.54 ± 17.36) ng/L vs .(123.35 ± 16.46) ng/L] and PLGF [ (217.49 ± 26.32) ng/L vs .(128.32 ± 21.23) ng/L] in HDCP group during mid period (24th week) , P<0. 01 all ,and serum VEGF and PLGF levels more significantly reduced during late pregnancy (34th week) (P<0.01);in HDCP group ,compared with gestational hypertension group and severe preeclampsia group ,PLGF level [(127.46 ± 16.55) ng/L ,(158.40 ± 26.33) ng/L vs .(99.02 ± 27.26) ng/L] significantly reduced in mild preeclampsia group on 24th week;the serum VEGF and PLGF levels more significantly reduced on 34th week , P<0. 05 or <0. 01 . Conclusion:Reduced serum levels of VEGF and PLGF during mid pregnancy in HDCP patients are help to early diagnosing HDCP .

Objective To study clinical characteristics and diagnostic methods of lymphoma with chest invovement. Methods Twenty-five lymphoma patients with chest involvement were retrospectively analysed, they were all diagnosed in Peking University Third Hospital during 2000 to 2007. The data were collected including clinical manifestations, blood examinations, chest X-ray and CT scan, diagnostic methods and pathologic diagnosis. Results The median age of the 25 patients was 46 years old. Pyrexia(13 cases), weight loss over 10 percent in 6 months(11 cases), cough(10 cases), shortness of breath(9 cases) and painless enlargement of the peripheral lymph nodes(16 cases) were common manifestations. Erythrocyte sedimentation rate and serum lactate dehydrogenase(LDH) level were increased in 72.7% and 81% patients, respectively. The enlargement of mediastinum lymph nodes(16 cases, 64%) was the most common presentation of chest radiography, followed by pulmonary involvement(15 cases, 60%) including infiltration or pulmonary consolidation, mass, multiple nodules, diffuse ground-glass shadow, miliary lesion. There were also presentations of pleural effusion(10 cases, 40%), pericardial effusion(4 cases, 16%), chest wall mass(2 cases, 8%). Eighteen patients(72%) had at least two kinds of these presentations. The appearance of pleural effusion were yellow turbid, bloody or chyliform. Rivaha tests were all positive. The median value of plearal effusion examinations were listed as follows: specific gravity 1.031, total cells 9800×10~6/L, WBC 6.72×10~9/L, lymphocyte 86%, neutrophil 14%, protein 31.4 g/L, LDH 296 U/L,adenosine deaminase (ADA) 67.4 U/L Most patients(16 cases) were diagnosed by surgical biopsy,especialy peripheral lymph nodes biopsy (12 cases). Other patients were diagnosed by ultrasound or CT-guided biopsy (5 cases), video-assisted thoracoscopic pleural biopsy (1 case), video-mediastinoscopic mediastinum lesion biopsy(1 case), bronchial mucosa biopsy through bronchoscope(1 case), bone marrow examination(1 case). All the cases were non-Hodgkin lymphoma except one. Conclusions There was no specific clinical manifestation for lymphoma with chest involvement, but in almost half of patients there were enlargement of not only peripheral but also mediastinum lymph nodes. And there were some characteristics in serum, pleural effusion, chest X-ray and CT scan. Surgical biopsy of peripheral lymph nodes was a simple and convenient method for diagnosis. Micro-invasive biopsy had good diagnostic value for lymphoma with chest involvement, including ultrasound-or CT-guided biopsy for superficial mass, pleura, lung, liver, spleen and deep lymph nodes, video-assisted thoracoseopic and video-mediastinoscopic biopsy for pleura, lung and mediastinum lesions. But bronchial mueosa and lung biopsy during bronchoscopy had a low diagnostic rate for lymphoma.

After the concept and characteristics of public health emergencies were described , the advantages and possibility of medical library to provide service for public health emergencies were analyzed with suggestions put for-ward for medical library to provide service for public health emergencies,such as perfecting emergency measures and service mechanisms ,cultivating active and advance service awareness ,developing special database ,establishing close communication with related institutions, enforcing network construction and maintenance,and improving librarians'literacy.

Objective To discuss the correlation factors of locally recurrent gastric carcinoma. Methods Clinical, pathological and periodical follow-up datas of 50 cases with locally recurrent gastric carcinoma after radical resection from 1991 to 2005 were studied retrospectively, logistic regression analysis was used to disscuse the possible correlation factors of locally recurrent gastric carcinoma. Results 66.0% of 50 cases had recurrenced within 2 years after operations; Tumor pathological stages, positive lymph nodes and radical resection extent were independent correlation factors of locally recurrent gastric carcinoma. Conclusions Most recurrences would happen within 2 years after radical resection for gastric carcinoma; Early diagnosis of reccurrent gastric carcinoma depends on periodic follow-up observation especially gastroscope; Tumor pathological stages, positive lymph nodes and radical resection extent are independent correlation factors of locally recurrent gastric carcinoma.

Objective To explore the influence of the sequential enteral nutrition(SEN) on nutritional status and prognosis in patients with acute cerebral infarction(ACI).Methods 80 ACI patients complicated with dysphagia were divided into SEN group and control group(40 cases in each group).In the first three days patients in SEN group were treated with nasal feeding short-peptide enteral nutrition[84~126(kJ/kg.d) ],than,which would be replaced with nasal feeding integral-protein enteral nutrition from the forth day.Patients in the control group were provided with ordinary fluid food by nasal feeding.Nutrition support for both groups was at last more than 10 d.In the 1 d,7 d and 14 d after treatment,data of both groups concerned with triceps skin fold(TSF),mean upper arm muscle circumference of triceps(MAMC),serum albumen(Alb),serum prealbum(PAB),heamoglobin(Hb),incidence of infectious complications,National Institutes of Health Stroke Scale(NIHSS) score and Barthel index(BI) were examined.Results Nutritional indexes(TSF,MAMC,Alb,PAB,Hb) in the SEN group were significantly higher than those in the control group(P

Humans ; Pancreatic Neoplasms ; prevention & control ; therapy

To use Agrobacterium rhizogenes-induced hairy roots to create new germplasm of Dianthus caryophyllus, we transformed D. caryophyllus with A. rhizogenes by leaf disc for plant regeneration from hairy roots. The white hairy roots could be induced from the basal surface of leaf explants of D. caryophyllus 12 days after inoculation with A. rhizogenes ATCC15834. The percentage of the rooting leaf explants was about 90% 21 days after inoculation. The hairy roots could grow rapidly and autonomously in liquid or solid phytohormone-free MS medium. The transformation was confirmed by PCR amplification of rol gene of Ri plasmid and silica gel thin-layer chromatography of opines from D. caryophyllus hairy roots. Hairy roots could form light green callus after cultured on MS+6-BA 1.0-3.0 mg/L + NAA 0.1-0.2 mg/L for 15 days. The optimum medium for adventitious shoots formation was MS + 6-BA 2.0 mg/L + NAA 0.02 mg/L, where the rate of adventitious shoot induction was 100% after cultured for 6 weeks. The mean number of adventitious shoot per callus was 30-40. The adventitious shoots can form roots when cultured on phytohormone-free 1/2 MS or 1/2 MS +0.5 mg/L NAA for 10 days. When the rooted plantlets transplanted in the substrate mixed with perlite sand and peat (volume ratio of 1:2), the survival rate was above 95%.
Agrobacterium ; Chromatography, Thin Layer ; Culture Media ; Dianthus ; growth & development ; Plant Growth Regulators ; Plant Leaves ; Plant Roots ; growth & development ; Plants, Genetically Modified ; Rhizobium ; Tissue Culture Techniques ; Transformation, Genetic

Objective To evaluate and monitor the efifcacy of GnRH analogs (GnRHa) therapy. Methods Thirty girls with central precocious puberty diagnosied by LHRH stimulation test were treated with GnRHa for 6-24 months. The LHRH stimula-tion test were performed again at 3 months after initiation of therapy and then every 6 months during treatment. The relationship of peark LH and clinical suppressing pubertal (including Turner stage, bone age, grwoth speed) were compared. The monitor effect of peak LH to efifcacy of GnRHa were eveluated. Results Ninety LHRH stimulation tests were performed, only 7 cases were found to have clinical pubertal development. After 6 months treatment, the base LH level of thirty girls (0.48±0.20) IU/L was signiifcantly lower than that before the treatment (0.75±0.35 IU/L) (P=0.000). The correlation coefifcient between base LH and peak LH was 0.62. The best correlation between clinical suppressing pubertal and LHRH stimulation test was achieved when peak LH was less than 2 IU/L (85.7%sensitivity, 100%speciifcity). Conclusions Base LH value can be used in preliminary as-sessment of the efifcacy of GnRHa therapy for girls with central precocious puberty. The peak LH less than 2 IU/L can be as the indicator of treatment efifcacy.

To observe the transcriptional regulation of the two isoflavones genistein and daidzein on target genes.