In 2002-2003 year period at HCM City Blood Transfusion and Hematology 8 cases of umbilical cord blood transplantation were performed.including 2 cases of lymphoid acute leukemia, 3 cases of myeloid acute leukemia, 3 cases of beta thalassemia. Among them, there were a case of HLA- identical blood and 7 cases of HLA-dismatched blood. After 36 months, 61,90% of cases were recovered survivals and after 24 months 71,43%
Stem Cell Transplantation ; Epidemiology ; Vietnam ; Cord Blood Stem Cell Transplantation

Blood screening voluntary donor in HCMC. We investigated 47,621 samples of blood. Results: HbsAg (+): 7.15%, antiHCV: 3.11%, anti HIV: 0.29; VDRL (+) 1.08%, Malaria (+): 0.27%; abnormal antibodies: 0.20%.
Blood Donors ; Hepatitis C Antibodies ; Hepatitis B Surface Antigens ; Anti-HIV Agents

203 products of total blood and 203 products of the erythroprecipitin undergone the quality control during 1/200-27/9/2000. The results have shown that the blood volume of 4.92% products was lower than 250 ml and level of hemoglobin of 1% products was lower than 22g among 203 controlled products of the total blood; volume of 2.11% products was less than 125ml and of 12.67% products was more than 125 ml; the level of hemoglobin of 100% products was higher than 22 g and of 80% products was higher than 25g, the hematocrite of 4.22% products was higher than 0.75 l/l among 142 controlled products of the erythroprecipitin. The conclusion: products, in generally obtained the ISO 9002.
Quality Control ; blood ; Blood Platelets ; Erythrocytes

We performed 10 cases of autologous peripheral blood stem cell transplantation without cryoprevervation in 11/1996 and 12/1997 12. Patients were diagnosed of AML, ALL, CML and non Hodgkin’s lymphoma. The median age of the patients was 34 years (range 18-43). Peripheral blood stem cell mobilizing regiments consisted of Daunorubicine (50 mg/ m2/d, d1-d5) cytarabine and etoposide in 3 CML patients and high dose cyclophosphamide in the seven other patients, followed by G-CSF starting when an ANC< 0.5x 109/l until the day before the last leukapheresis. When a WBC count reached 4x109/l leukapheresis was started and continued for 3 consecutive days, as soon as the third collection was finished Melphalan was given as a conditioning regimen, 42 hours after giving Melphalan, the collected PSCs which were preserved at +40C (90 hours after giving Melphalan, the collected PSCs which were preserved at + 40C were reinfused through a central Hickman’s catheter. GM-CSF was given during the nadir period until the an ANC > 1x109/l for 3 consecutive days. The median number of mononucleated cells and CD34+ cell were 5x108/kg respectively. All platelet count > 20 x 109/l was 13 days and 20 days following transplantation, all patients are still in CR and alive with the median follow-up of 8 months.
Transplantation ; blood ; Hematologic Diseases

The study was carried out on 98 patients who needed to be transfused regularly. Phenotyped red blood cells of groups were used for this purpose. Only immunogenic antigens of the common blood groups were included in this study, such as Rh, MNSs, Duffy and Kidd systems. The highest quantity of units transfused depassed the number 44. After a period of thirteen months, there was no antibody detected. But in the other hand, we found 4 cases of immune antibodies in patients who previously received multiply transfusions of non phenotyped blood. These antibodies belong to Rh, Duffy and Kidd systems which are highly immunogenic. These results showed the need and importance of performing adequate compatibility testing for multiply transfused patients and the benefit of using phenotyped blood for those who need regular transfusions.
Phenotype ; Blood transfusion

The blood transfusion is necessary while the donor’s marrow has not been growed yet. The transfused blood products must assure completely the safety principles for disease transmission, the blood products is not infected the MCV and removed the leucocyte usually transfused after 4- 12 weeks of transplantation with the suitable dosage for individuals. Condensed platalet is transfused after 6-12 weeks of transplantation. It should maintain the amount of thrombocyte in the peripheral blood within 50-100x109/lit, the dose is different from one patient to another.
Blood transfusion ; Bone marrow ; Transplantation

The bone marrow transplantation is a most modern technique and applied in Vietnam to treat the malignant hematological diseases. This paper introduced the bone marrow heterotransplantation and the autologous peripheral blood stem cell transplantation. 3 patients (1 male; 2 female) received transplantation (bone marrow fluid was extracted from patient’s brothers) for the treatment of the chronic myeloid leukemia and severe thalassemia and 17 patients received the autologous peripheral blood stem cell transplantation for treatment of acute myeloid leukemia, chronic myeloid leukemia, acute lyphoid leukemia and lymphoma. The results have shown that the longest survival duration was 42 months after the autologous peripheral blood stem cell transplantation. The patients had a normal life such as learning, working and social activities. After 2 months of transplantation, the average disease free duration of 10 patients with AML and 6 patients with ALL were 27 months, and 30-40 months, respectively. This was an great encouragement.
Bone Marrow Transplantation ; Bone marrow ; Bone and bones

In this paper, a total of 467 species of medicinal plant, belonging to 109 families have been reported used by Yao (Dao) ethnic minority living in Bavi National Park’s buffer zone. Forty-four species have been identified as frequently used and twenty-nine species of high priority for conservation. Principal Components Analysis revealed that abundance of medicinal plants and number of medicinal plants listed in the Vietnamese Essential Drug List are negatively, significant correlated to PCA1 which dominated by altitude, slope, vegetation disturbance, number of tree species, canopy height of trees and grass cover. While number of high priority conservation and number of frequently used medicinal plants are positively correlated with PCA1.
In this paper, a total of 467 species of medicinal plant, belonging to 109 families have been reported used by Yao (Dao) ethnic minority living in Bavi National Park’s buffer zone. Forty-four species have been identified as frequently used and twenty-nine

Induction chemotherapy of 7-3-5 protocol (cytarabine, daunorubicin, etoposide) in acute myeloid leukemia. Between October 1998 and October 1999, 10 patients with AML (4M, 6F, median age 31) were treated with induction chemotherapy of 7-3-5 protocol at the blood transfusion and hematology center, HCM city. A complete remission was achieved in 9 of 10 patients. 1 patient died from transfusion- associated graft- versus- host disease. The median time to recover ANC> 05x 109/1 and platelets> 20x109/1 were 16 days. Oral mucositis was mild in 2 patients. 7-3-5 protocol may be more effective than 7-3 protocol in response rate and prolonged remission but toxicity was similar.
Leukemia, Myelocytic, Acute ; therapy ; therapeutics

From 11/1997 to 8/2000, 11 patients with acute myeloid leukemia (AML) were performed autologous peripheral blood stem cell (PBSC) transplantation without cryopreservation. PBSC were mobilized by high-dose cyclophosphamide plus granulocyte colony-stimulating factor.When WBC count reached>5x109/l, leukapheresis was started and continued for 3 consecutive days. As soon as the third collection was finished, mephalan was given as a conditioning regimen. 24-42 hours after giving melphalan, the collected PBSC which were preserved at +40C were reinfused. Granulocyte macrophage colony-stimulating factor was given during the nardir period until the an ANC> 1x 109/l for 3 consecutive days. All the patients were engrafted with the mean no time to recover ANC> 0.5x109/l and platelet count > 20x109/l was 14 days and 33.8 days. Overall survival and disease free survival at 2 years were 77.7% and 76.1% respectively. In conclusion, autologous PBSC transplantation without cryopreservation in AML is simple and economic method which may be applied in developing countries.
Leukemia, Myelocytic, Acute ; Peripheral Blood Stem Cell Transplantation