1.Morphometric Analysis of the Skull by Moire Contourography.
Duk Soo KIM ; Jeong Sik KO ; Kyung Ho PARK ; Dae Kyoon PARK
Korean Journal of Physical Anthropology 2014;27(3):165-171
The non-metric analysis of the skulls is very useful for estimating sex and determination of ancestry, the accuracy tends to depend on the amount of experiences of the observers, and so inter-observer errors might be happened. Many researchers are trying to find out more objective methods for determination of ancestry. The purpose of this presentation is to show the usefulness of moire contourography for analyzing the skull. The master screen that is similar to the gratings was made by steel rods, which were arranged as equally spaced parallel lines. Halogen light source was illuminated by lantern slide projector. The skeletal materials were documented crania, composed of 87 male and 47 female, from William M. Bass Donated Skeletal Collection housed at the Department of Anthropology, University of Tennessee. The skulls were placed just behind the master screen as anatomical position using cubic craniophore. The angle between the light source and camera was 65degrees, the distance between camera and the master screen was 1.2 m. Frontal view, left lateral and right lateral view were taken. From the frontal view, fringe patterns were analyzed for first five contour lines which were mainly located around the Glabella. The results were as followed; Type I for male was 53% and female was 4%; Type II for male was 29% and female was 2%; Type III for male was 2% and female was 15%; Type IV for male was 6% and female was 55%. From the lateral view, fringe patterns were analyzed for first four contour lines. However, first and second contour lines were critical to determine the shape and the results were as followed; Type I for male was 52% and female was 22%; Type II for male was 38% and female was 26%; Type III for male was 8% and female was 17%; Type IV for male was 2% and female was 35%. According to this study, different fringe patterns might be dependent on the degree of development of bone marker such as Glabella, Supercillary arch, Euryon and Mastoid process. For example, Supercillary arches were very well developed and slope of forehead above the Glabella was declined, fringe pattern showed reverse triangle shape. If Supercillary arches were poorly developed and slope of forehead above the Glabella was flat, fringe pattern showed home plate shape. The present research shows that moire contourography might be used as more objective methods for estimating sex. And it would be helpful to determine the ancestry when the lateral aspects were analyzed. In the future, continuing study need to be performed with same master screen for different ancestry.
Anthropology
;
Bass
;
Female
;
Forehead
;
Humans
;
Male
;
Mastoid
;
Skull*
;
Steel
;
Tennessee
2.Concept Analysis of Volition for Health Behavior Practice in Patients with Chronic Disease.
Journal of Korean Academy of Nursing 2016;46(5):687-696
PURPOSE: The purpose of this study was to define and clarify the concept of volition for health behavior. METHODS: For this study the process of Walker & Avant’s concept analysis was used. Seventeen studies from electronic data basses met criteria for selection. RESULTS: Volition can be defined by the following attributes: 1) planning, 2) maintenance of self-efficacy, and 3) self regulation. The antecedents of volition consisted of: 1) risk awareness, 2) outcome expectation, 3) perceived self-efficacy, and 4) social support. The consequences occurring as a result of volition were: 1) prevention of disability and complications, 2) improvement of functional ability, and 3) enhencement of quailty of life. CONCLUSION: Definition and attributes of volition identified by this results can be applied to develop measurements and intervention programs for chronic patients health behavior.
Bass
;
Chronic Disease*
;
Health Behavior*
;
Humans
;
Self-Control
;
Volition*
;
Walkers
3.Effect of the education interval and method on improving Patients' plaque control ability.
Do Young PARK ; Byoung Jin LEE ; Byung Ock KIM ; Sang Joun YU
Journal of Korean Academy of Oral Health 2015;39(2):145-151
OBJECTIVES: This study aimed to evaluate the effect of oral health education interval and toothbrushing instruction, the difference between the demonstration-only method and the demonstration with chairside practice toothbrushing instruction method, and the effect of initial patient plaque control ability on the plaque index. METHODS: Patients (n=60) were randomly assigned to receive a 1-week, 2-week, or 3-week interval of oral health education and toothbrushing instruction. Each group was further subdivided into the demonstration-only group and the demonstration with chairside practice group. Patients were categorized as having "good", "fair", or "poor" initial plaque control ability, based on the Turesky modification of the Quigley-Hein plaque index (TQHI). Patients attended five sessions during which they received oral health education and toothbrushing instruction of the modified Bass technique. Plaque evaluation was performed at each visit using the TQHI. RESULTS: The plaque index tended to improve from the first visit to the fifth visit, but there were no significant differences between the 1-week, 2-week, and 3-week education interval. The demonstration with the chairside practice group showed significantly greater improvements in the plaque index, compared to the demonstration-only group. In the good, fair, and poor plaque control ability groups, the plaque index improved gradually from the first to the fifth visit. When plaque control was poor, the improvement in the plaque index increased to a greater degree after oral health education and toothbrushing instruction. CONCLUSIONS: The results of this study may be helpful for suggesting appropriate individualized oral hygiene management methods to improve plaque control ability.
Bass
;
Dental Plaque
;
Dental Plaque Index
;
Education*
;
Humans
;
Oral Health
;
Oral Hygiene
;
Toothbrushing
4.Infections with Digenean Trematode Metacercariae in Two Invasive Alien Fish, Micropterus salmoides and Lepomis macrochirus, in Two Rivers in Chungcheongbuk-do, Republic of Korea
Seongjun CHOE ; Hansol PARK ; Dongmin LEE ; Yeseul KANG ; Hyeong Kyu JEON ; Keeseon S EOM
The Korean Journal of Parasitology 2018;56(5):509-513
Present study was performed to survey infection status of digenetic trematode metacercariae in 2 alien fish species, Micropterus salmoides (largemouth bass) and Lepomis macrochirus (bluegill), in 2 rivers draining Chungcheongbuk-do, Republic of Korea. A total of 107 largemouth bass and 244 bluegills were caught in Daecheong-ho (ho=lake) and Musim-cheon (a branch of Geum-gang), in Chungcheongbuk-do April–July 2015. Additionally, 68 native fish of 5 species, i.e., Zacco platypus, Hemibarbus longirostris, Carassius auratus, Pseudogobio esocinus and Puntungia herzi, were caught from the same water bodies. All of the fish collected were examined by artificial digestion method. The metacercariae of Centrocestus armatus, Clinostomum complanatum, Metagonimus sp. and Diplostomum spp. were detected from 4 out of 5 native fish species in Daecheong-ho. However, any metacercariae were not found from 87 M. salmoides and 177 L. macrochirus in Daecheong-ho. In Musim-cheon, metacercariae of Exorchis oviformis and Metacercaria hasegawai were detected from 78% Z. platypus and 34% L. macrochirus, but any metacercariae not found in M. salmoides. We report here that the 2 alien fish species were less infected with the metacercariae than the native ones. Surveys on the metacercariae in the alien fish species in geographically various rivers should be undertaken for better understanding on the role of alien fish species in the trematode infections in Republic of Korea.
Bass
;
Chungcheongbuk-do
;
Digestion
;
Emigrants and Immigrants
;
Goldfish
;
Heterophyidae
;
Humans
;
Metacercariae
;
Methods
;
Perciformes
;
Platypus
;
Republic of Korea
;
Rivers
;
Trematode Infections
;
Water
5.Musculoskeletal Disorders and Related Factors of Symphony Orchestra Players.
Nak Jung SUNG ; Joon SAKONG ; Jong Hak CHUNG
Korean Journal of Occupational and Environmental Medicine 2000;12(1):48-58
OBJECTIVES: Several studies have shown that performance related musculoskeletal disorders present a significant and common health problem for instrument players. This study was conducted to investigate the symptom prevalence rate of performance-related musculoskeletal disorders and risk factors on symphony orchestra players. METHODS: The symptom prevalence rate of musculoskeletal disorders and related factors including demographic factors, occupational factors, psychosocial factors, practice and performance-related behaviors of on 156 symphony orchestra players in Taegu city were surveyed with a self-administered questionnaire. RESULTS: Instrument players have performed for 14. 6 years in average and worked 4. 9 for years in average as a member of symphony orchestra. The subjects consisted of 106 string players, 48 wind players, and 8 percussion players. The symptom prevalence rate of musculoskeletal disorders according to the NIOSH surveillance criteria was 79. 6 % and operational criteria by authors was 45. 9 %. The prevalence rate of viola players was higher than violin players, and the prevalence rate of the bass players was higher than that of the cello players. In univariate analysis, job insecurity, irregular performance, stress from family problems, and performance related psychosomatic strains had a significant association with the prevalence rate of musculoskeletal disorders. In multiple logistic regression, job insecurity, irregular performance, and stress from family problems were significant variables affecting the prevalence rate of musculoskeletal disorders of instrument players. CONCLUSIONS: This study suggest that symphony orchestra players are high risk group of musculoskeletal disorders. Irregular performance schedule and psychosocial stress including job insecurity, and psychosomatic strain were important risk factors of players musculoskeletal disorders. An exercise program such as stretching before and after performance, regular performance schedule and reduction of psychosocial stress might be helpful for prevention of musculoskeletal disorders of instrument players.
Appointments and Schedules
;
Bass
;
Daegu
;
Demography
;
Humans
;
Logistic Models
;
National Institute for Occupational Safety and Health (U.S.)
;
Percussion
;
Prevalence
;
Psychology
;
Questionnaires
;
Risk Factors
;
Viola
;
Wind
6.Plasma Paraquat Concentration and Early Prognostic Factors in Paraquat Poisoning.
WonSuk CHOI ; Ja Hyun KIM ; Hyun A LEE ; KyungHong JUN ; MyungSoo KANG ; Hyang KIM
Korean Journal of Nephrology 2010;29(5):554-561
PURPOSE: Paraquat is a lethal herbicide and induces acute renal failure, hepatic dysfunction, and progressive respiratory failure. The aims of this study are to investigate the correlation between plasma paraquat concentrations and initial laboratory data at Emergency Medical Center and to investigate whether initial laboratory data is useful for predicting outcomes of paraquat-poisoned patients. METHODS: A retrospective analysis by chart review was done on 83 patients who ingested paraquat and who had presented to Emergency center of within 24 hours. Plasma paraquat concentrations, urine dithionite test and initial laboratory parameters including white blood cell count, urine pH, and AST, ALT, BUN, Creatinine, Amylase, Glucose, pH, PaCO2, PaO2, HCO3. Base Excess, Na, K, Cl were obtained at the time of Emergency Center visit. We compared urine dithionite test, plasma paraquat concentrations and Severity Index of Paraquat Pisoning (SSPI) of the survival group to those of the dead group. The patients were divided into four subgroups based on the level of plasma paraquat concentration, their initial laboratory data was compared and analyzed. RESULTS: The mean plasma paraquat concentration in the mortality group was higher than that in the survival group (88.44+/-81.56 vs. 1.32+/-1.72 microgram/mL). Among the initial laboratory data of four subgroups, WBC, Glucose, Cr, pH, HCO3, Bass excess were significantly different between the group of low level of plasma paraquat concentration and higher group. ANCOVA analysis revealed that WBC, HCO3, Bass excess correlated with the level of plasma paraquat concentration significantly. CONCLUSION: The plasma paraquat concentration and SIPP were higher in the mortality group significantly. Initial laboratory data including WBC, Glucose, Cr, pH, HCO3, Bass excess were proven to be significant prognostic factors. Especially WBC, HCO3, Bass excess can be used to predict the outcome of paraquat poisoning.
Acute Kidney Injury
;
Amylases
;
Bass
;
Creatinine
;
Dithionite
;
Emergencies
;
Glucose
;
Humans
;
Hydrogen-Ion Concentration
;
Leukocyte Count
;
Paraquat
;
Plasma
;
Prognosis
;
Respiratory Insufficiency
;
Retrospective Studies
7.Dental Hygienist-Led Dental Hygiene Process of Care for Self-Support Program Participants in Gangneung.
Sang Hee YOO ; Seon Hui KWAK ; Sue Hyang LEE ; Ga In SONG ; Soo Myoung BAE ; Sun Jung SHIN ; Bo Mi SHIN
Journal of Dental Hygiene Science 2018;18(6):327-339
This study aimed to provide basic data for establishing the clinical basis for dental hygienist-led dental hygiene process of care by identifying multiple risk factors for self-support program participants in Gangneung city; we also compared oral health status and behavioral changes through customized oral health care. Four dental hygienists who were evaluated for degree of conformity provided dental hygiene process of care to eight self-support program participants who were selected as having an oral health risk among people in the self-support center. The clinical indicators measured during dental hygiene assessment and evaluation and behavioral changes due to dental hygiene intervention were compared and analyzed. With respect to clinical indicators, at the time of probe, the retention rate of patients with gingival bleeding decreased from 61.4% to 14.7% after intervention (p=0.004). Furthermore, the retention rate of patients with a periodontal pocket >4 mm decreased from 15.6% to 5.8% (p=0.001). The average modified O'Leary index of the patients improved from 23 to 40 (p=0.002). Previously, all eight subjects used the vertical or horizontal method of brushing; after dental hygiene care interventions regarding method and frequency of toothbrushing, use of oral care products, and individual interventions, they started using the rolling or Bass method of toothbrushing. Four of eight subjects reported using interdental toothbrushes after intervention. As a result of applying the change model to the transtheoretical behavior change of the subject, the result of strengthening the health behavior was confirmed. For promotion of oral health by the prevention-centered incremental oral health care system, dental hygienist-led dental hygiene management and maintenance is essential. It is thought that continuous research, such as for feasibility evaluation, cost benefit analysis, and preparation of legal systems, is needed to establish and activate dental hygiene management.
Bass
;
Cost-Benefit Analysis
;
Dental Hygienists
;
Dental Prophylaxis
;
Gangwon-do*
;
Health Behavior
;
Hemorrhage
;
Humans
;
Methods
;
Oral Health
;
Oral Hygiene*
;
Periodontal Pocket
;
Risk Factors
;
Toothbrushing
8.Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system.
Sun-Jian LYU ; Xue-Mei YUAN ; Hai-Qi ZHANG ; Wei-da SHI ; Xiao-Ying HANG ; Li LIU ; Ying-Lei WU
Journal of Zhejiang University. Science. B 2019;20(9):728-739
As one of the most important aquatic fish, Micropterus salmoides suffers lethal and epidemic disease caused by rhabdovirus at the juvenile stage. In this study, a new strain of M. salmoides rhabdovirus (MSRV) was isolated from Yuhang, Zhejiang Province, China, and named MSRV-YH01. The virus infected the grass carp ovary (GCO) cell line and displayed virion particles with atypical bullet shape, 300-500 nm in length and 100-200 nm in diameter under transmission electron microscopy. The complete genome sequence of this isolate was determined to include 11 526 nucleotides and to encode five classical structural proteins. The construction of the phylogenetic tree indicated that this new isolate is clustered into the Vesiculovirus genus and most closely related to the Siniperca chuatsi rhabdovirus. To explore the potential for a vaccine against MSRV, a glycoprotein (1-458 amino acid residues) of MSRV-YH01 was successfully amplified and cloned into the plasmid pFastBac1. The high-purity recombinant bacmid-glycoprotein was obtained from DH10Bac through screening and identification. Based on polymerase chain reaction (PCR), western blot, and immunofluorescence assay, recombinant virus, including the MSRV-YH01 glycoprotein gene, was produced by transfection of SF9 cells using the pFastBac1-gE2, and then repeatedly amplified to express the glycoprotein protein. We anticipate that this recombinant bacmid system could be used to challenge the silkworm and develop a corresponding oral vaccine for fish.
Animals
;
Baculoviridae/metabolism*
;
Bass/metabolism*
;
Carps/virology*
;
Cell Line
;
Female
;
Genetic Techniques
;
Genome, Viral
;
Glycoproteins/biosynthesis*
;
Insecta
;
Ovary/virology*
;
Phylogeny
;
Plasmids/metabolism*
;
Recombinant Proteins/biosynthesis*
;
Rhabdoviridae/metabolism*
9.In vivo morphological and antigenic characteristics of Photobacterium damselae subsp. piscicida.
Tae S JUNG ; Kim D THOMPSON ; Donatella VOLPATTI ; Marco GALEOTTI ; A ADAMS
Journal of Veterinary Science 2008;9(2):169-175
The present study was conducted to examine the morphology and antigenicity of Photobacterium damselae subsp. piscicida by culturing the bacterium in vivo in the peritoneal cavity of sea bass (Dicentrarchus labrax) within dialysis bags with either a low molecular weight (LMW) cut-off of 25 kDa or a high molecular weight (HMW) cut-off of 300 kDa. Differences were observed in the growth rate between the bacteria cultured in vivo or in vitro. Bacteria cultured in vivo were smaller and produced a capsular layer, which was more prominent in bacteria cultured in the HMW bag. Antigenicity was examined by Western blot analysis using sera from sea bass injected with live Ph. d. subsp. piscicida. The sera recognised bands at 45 and 20 kDa in bacteria cultured in vivo in the LMW bag. Bacteria cultured in vivo in the HMW bag did not express the 45 kDa band when whole cell extracts were examined, although the antigen was present in their extracellular products. In addition, these bacteria had a band at 18 kDa rather than 20 kDa. Differences in glycoprotein were also evident between bacteria cultured in vitro and in vivo. Bacteria cultured in vitro in LMW and HMW bags displayed a single 26 kDa band. Bacteria cultured in the LMW bag in vivo displayed bands at 26 and 27 kDa, while bacteria cultured in vivo in the HMW bag possessed only the 27 kDa band. These bands may represent sialic acid. The significance of the changes observed in the bacterium's structure and antigenicity when cultured in vivo is discussed.
Animals
;
Antigenic Variation/*genetics
;
Antigens, Bacterial/genetics/*immunology
;
Bass/*immunology/microbiology
;
Blotting, Western
;
Carbohydrates/analysis
;
Electrophoresis, Polyacrylamide Gel
;
Membranes, Artificial
;
Microscopy, Electron, Transmission
;
N-Acetylneuraminic Acid/genetics/*immunology
;
Photobacterium/genetics/*immunology/ultrastructure
10.Variation in the molecular weight of Photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro.
Tae S JUNG ; Kim D THOMPSON ; Donatella VOLPATTI ; Marco GALEOTTI ; A ADAMS
Journal of Veterinary Science 2007;8(3):255-261
The antigenicity of Photobacterium damselae (Ph. d.)subsp. piscicida, cultured in four different growth media[tryptone soya broth (TSB), glucose-rich medium (GRM),iron-depleted TSB (TSB+IR-), and iron-depleted GRM(GRM+IR-)] was compared by enzyme-linked immuno-sorbent assay (ELISA) and Western blot analysis usingsera obtained from sea bass (Dicentrarchus labrax) raisedagainst live or heat-killed Ph. d. subsp. piscicida. Theantigenic expression of Ph. d. subsp. piscicida was found todiffer depending on the culture medium used. A significantlyhigher antibody response was obtained with iron-depletedbacteria by ELISA compared with non-iron depletedbacteria obtained from the sera of sea bass raised againstlive Ph. d. subsp. piscicida. The sera from sea bass raisedagainst live bacteria showed a band at 22kDa in bacteriacultured in TSB+IR- or GRM+IR- when bacteria thathad been freshly isolated from fish were used for thescreening, while bands at 24 and 47kDa were observedwith bacteria cultured in TSB or GRM. When bacteriawere passaged several times on tryptic soya agar prior toculturing in the four different media, only bands at 24 and47kDa were recognized, regardless of the medium used toculture the bacteria. It would appear that the molecularweight of Ph. d. subsp. piscicida antigens change in thepresence of iron restriction, and sera from sea bassinfected with live bacteria are able to detect epitopes onthe antigens after this shift in molecular weight.
Animals
;
Antibodies, Bacterial/blood
;
Antigens, Bacterial/immunology/*metabolism
;
Bass/blood/*immunology
;
Blotting, Western/veterinary
;
Cell Count/methods
;
Culture Media
;
Enzyme-Linked Immunosorbent Assay/veterinary
;
Fish Diseases/immunology/*microbiology
;
Molecular Weight
;
Pasteurella Infections/immunology/microbiology/*veterinary
;
Photobacterium/*immunology