Objective To detect the chromosomal aberration in the cells of renal carcinoma and to evaluate the rela-tionship between the chromosomal aberration and development of renal carcinoma.Methods CGH technology was used to analyze the global genomic aberration in cancer cells from 12 patients with renal carcinoma.Results All of 12 cases deeeted by CGH showed chromosomal aberrations.The common extension regions of renal carcinoma were 1p,4p,5q,7p,9p and 16p.The common deletion regions of renal carcinoma were 3q,4q,6q,9q,14q and 18q.Conclusion The hereditary material of renal carcinoma is unbalanced.The extension and/or deletion of chro-mosome may facilitate the occurrence of renal carcinoma.

Objective To study the effect of interleukin 2(IL-2)of clinical dose range,on the proliferation of human peripheral blood T cells,with special emphasis on the number and functional phenotype of γδT cells.Methods Human peripheral blood mononuelear cells(PBMCs)were isolated by density gradient centrifugation and cultured for 2 weeks at different IL-2 concentrations.Ratio and phenotype of different T cell subpopulations before and after in vitro expansion were explored by immunofluorescence staining.Cell number was estimated by trypan blue staining and cell counting.Results Within the four functional phenotypes of Vδ1 as well as Vδ2 γδT cells,CD27~+cells(including CD27~+CD45RA~+and CD27~+CD45RA~-subsets)expressed lymphoid tissue homing receptor CCR7,whereas CD27~-cells(including CD27~-CD45RA~+and CD27~-CD45RA~-subsets)had the peripheral tissue homing potential.All the studied γδT functional subsets showed the expression of activity related receptors,and the ability of a rapid production of various amount of cytotoxicity related effectors following mitogen stimulation.Although IL-2 at high concentration suppressed the proliferation of CD4 T cells,it may promote the proliferation of γδT cells.The proliferated γδT cells were mainly CD27~-CD45RA~-effector cells.Conclusion IL-2 of the clinical dose range may promote proliferation of human peripheral blood γδT cells,which might have important biological significance in IL-2 based anti-tumor therapy.

Objective To observe the changes in neurotrophic factors and their receptors in the lumbar spinal cord of aging rats (1, 3, 6, 12, 18 and 24 months) , and to investigate their correlation with gowth. Methods The expression of neurotrophic factors and their receptors in the lumbar spinal cord of rats were measured by immunohistochemis-try and western blot. Nissl staining was used for quantitative detection of survival neurons. Results The expression of nerve growth factor (NGF) and its receptor TrkA, brain derived neurotrophic factor (BDNF) and its receptor TrkB and gliocyte derived neurotrophic factor (GDNF) in lumbar spinal cord of rats showed changes in relation to the aging process. Their expression was lower at the age of 1 month, reached the peak at 6-month, and decreased significantly at 24 months, in which the decline of GDNF was more obvious. The number of survival neurons in lumbar spinal cord kept at a high level from 1 to 6-month, then decreased gradually, and reached the lowest level at the age of 24 months. Conclusion The expression of neurotrophic factors and their receptors change with aging process, decreases in aged rats especially,which may be one of the mechanisms involved in spinal cord aging.

Objective To eonstruct three eukaryotic expression vectors containing wilde-type hGHR gene and its mutants(hGHR-E42K and hGHR-H56R) related to congenital growth hormone insensitivity, then check their expression in CHO cells. Methods With the available PUC-hGHR vector, two mutate hGHR genes (hGHR-E42K and hGHR-H56R) were obtained through mutagenesis. Then three recombinants were cloned into eukaryotic expression vectors pcDNA3.1/zeo(+) with restriction enzymatic reactions.Then with Lipofectamine2000, we trans-fected expression vectors to CHO cells and screened the stably expressed CHO cells by Zeocin. RT-PCR and/or Western blotting were used to examine hGHR and STAT5-P. Results After sequencing, two mutations were introduced to hGHR, three eukaryotic expression vectors were identified. The transfected CHO cells expressed vd-hGHR or its mutants. Compared with hGH-wt, two mutate cells (E42K and H56R) had decreased phosphorylated STAT5 levels. Conclusion Three CHO cells which stably expresses wide type hGHR and its mutants were successfully established, E42K and H56R partly interfered the phosphorylation of STAT5.

Gossypol is a kind of yellow polyphenolic compounds extracted from root, stem and seed of the cotton plant. It had received significant attention for its potential application as a male antifertility agent. Then it was used to treat female hormone-dependent diseases, for instance, endometriosis, hysteromyoma, uterine bleeding and dys-menorrheal. Current recent researches showed that gossypol has multiple biological activities, such as anti-inflammatory , antimalarial, antiviral, antioxidant activities and so on, especially the activity to induce tumor cell apoptosis.

Objective To observe the antioxidative capability and the mRNA expression of sodium pump αl-subunit in kidney of hypothyroid rats by iodine deficiency and illuminate the pathogenesis of kidney damage.Methods Wistar rats were randomly divided into two groups as control group (NT) and hypothyroid group (HT).The rats were all fed with low-iodine diet derived from an endemic goiter area and drank deionized water containing different potassium iodide to duplicate hypothyriod animal models.We determined the morphometric parameters of kidney by routine histology method.The contents of malondialdehyde and free radical scavengers (GSH-PX and SOD) in kid-ney,as well as the activity of Na~+-K~+ ATPase were measured in two groups.The mRNA expression of sodium pump α1 subunit was determined by reverse transcription-polymerase chain reaction.Results Compared with that in the control group,in hypothyroid group (1) serum free T3,free T4,total T3 and total T4 were markedly lower.(2) mean glomerular area and volum diminished markedly.(3)the content of MDA and activity of PGx increased markedly,but the activity of SOD decreased significantly,as well as the one of Na~+-K~+-ATPase.(4)the mRNA expression level of sodium pump α1 subunit was lower.Conclusion In a hypothyroid state,the decrease of antiox-idative capability of kidney resulted in lipid peroxidative damage,atrophy of kidney,decreased activity of Na~+-K~+ -ATPase and degression of sodium pump α1 subunit mRNA expression.

Objective To construct secretary recombinant adenoviral vector carrying the mouse prostate-specific mem-brane antigen gene through AdEasy vector system and the immunological outcome was assessed. Methods mPSMA was amplified from plasmid pCR-BluntⅡ-TOPO by PCR and subcloned into transfer vector pAdeno Vator CMV5, The signal peptide DNA sequence of hIL-2 was fused to 5'terminal of mPSMA gene to construct a secretary Ad-mPSMA. pAdv-mPSMA was co-transformed with pAdeno Vator △E1/E3 through homologous recombination. The recombinant adenoviruses were packaged, amplified and purified in HEK293 cells. HeLa cell was infected by recombinant ade-novirus Ad-mPSMA and the expression of mouse prostate-specific membrane antigen gene was detected by RT-PCR and Western blot. The recombinant adenovirus had been immuned mice, sera antibody against mPSMA from immu-nized mice was detected by ELISA. Results The secretary pAd-mPSMA was constructed successfully and typical cytopathic effect (CPE) was observed. The titer of the recombinant adenovirus was 1.32 × 10~(11)IU/mL and expres-sion of mPSMA was confirmed by RT-PCR and Western blot. The specific antibody against mPSMA had been found in serum of the immunized mice. Conclusion mPSMA gene recombinant adenovirus was constructed successfully, which provide a basis for further study on the anti-tumor immunotherapy role of PSMA.

Objective To compare the differences of cardiac function and interstitial remodeling between diastolic heart failure(DHF) and systolic heart failure(SHF) rabbit models. Methods To establish DHF model with abdo-mial aorta constriction and SHF model with abdomial aorta constriction plus aortic insufficiency. The cardiac func-tion was examined by UCG parameters and homodynamic parameters. The collagen content was measured through hydroxyproline colorimetric assay and shown as collagen area(CA), collagen volume fraction(CVF) and area ratio of Ⅰ to Ⅲ type collagen with PSR. Results Compared with control group, there were significantly increased thick-ness and stiffness of myocardium, impaired diastolic function but normal ejection fraction (EF), and significantly increased collagen content, CA, CVF and area ratio of Ⅰ to Ⅲ type collagen in DHF group; heart chamber was sig-nificantly enlarged, systolic function decreased, and collagen content, CA, CVF significantly increased, but ratio of Ⅰ to Ⅲ type collagen decreased in SHF group(P <0.05 or P <0.01). Conclusion DHF and SHF rabbit mod-els were established successfully, which can simulate clinical profiles and provide technical support to future re-search.

Objective To explore the effect of gene NDRG2 (N-myc downstream regulated gene 2) transfection on the proliferation and apoptosis of breast cancer cell. Methods Bcap37 cell line expressing low level product encoded by NDRG2 gene is transfected with recombinating adenovirus expressing high level NDRG2 gene product to improve NDRG2 gene expression level. NDRG2 expression was detected by Western blot and the proliferation activity of the cell lines was detected by MTT and flow cytometry. Results High level expression of NDRG2 inhibits cells growth and results in G_1 phase arrest in Bcap37 cell line. Compared with non-transfected Bcap37 cells and Bcap37 cells transfected by empty vector, apoptosis cells obviously increase in Bcap37 cell line transfected by recombinating adenovirus expressing high level NDRG2 gene, 12% after 48 hours and 21. 5% after 72 hours. Conclusion Overexpres-sion of NDRG2 in Bcap37 cells effectively inhibites cell proliferation and induces cell cycle arrest and apoptosis.

Objective To find the features and diagnostic values of CT for adrenal ganglioneuroma.Methods CT examinations were performed in 16 cases(7 with plain scan and 9 with both plain and enhanced scan).All cases were confirmed by pathological evidence.Results 2 cases showed solid -cystic mass,14 were solid mass,and 5 accompanied with calcification.The average CT value was from 20 to 40 HU.In 9 cases with enhanced scan,5 slightly enhanced on delayed phase and no enhancement was seen in the others.Conclusion Adrenal ganglioneuroma showed some characteristic CT appearance and CT scanning plays an important role in diagnosis of adrenal ganglioneuroma.