No abstract available.
Base Sequence*

EVB DNA fragments from 3 biopsies obtained from Vietnamese patients with NPC have been examined by PCR detection method, using a mixture of primers TH1,2. PRC products were cloned by using vector PCM TM II and were sequenced on Automated sequencer. The result showed that our PCR products were similar to the specific sequence of EBV have been reported in EMBL data library, confirmed that PCR products are specific fragments of EBV, we can successfully use the mixture of primers TH1,2 in PCR detection of EBV DNA in biopsies of NPC patients
Nasopharyngeal Neoplasms ; Base Sequence

Genomics ; Base Sequence ; China

No abstract available.
Base Sequence* ; DNA* ; Internet* ; Sequence Analysis, DNA*

Two species of cultivated Phellinus sp. were identified as P. baumii by internal transcribed spacer (ITS) sequence analysis. The fruit bodies of the examined strains were similar to those of naturally occurring strains, having a bracket-like form, yellow-to-orange color, and poroid hymenial surfaces. The DNA sequences of ITS region of both strains showed a homology of 99% with ITS1 to ITS2 sequences of P. (Inonotus) baumii strain PB0806.
Base Sequence ; Fruit ; Sequence Analysis ; Sprains and Strains

No Abstract Available.
Animals ; Base Sequence* ; Cattle* ; Serotyping*

No abstract available.
Antibodies, Monoclonal* ; Base Sequence* ; DNA*

OBJECTIVETo search the DNA sequences specific to virulent strain of Streptococcus mutans in the public database and explore new genes or new functions of already known genes from Streptococcus mutans of serotype c and suppose their functions.

METHODSThirty-one DNA fragments unique to virulent strain of Streptococcus mutans were sequenced. The sequences of these presumptive virulence DNA fragments were subjected to search through software BLASTn and BLASTx in public database, and their putative biological functions were analyzed. RESULTS Two clones were picked repeatedly. The size of the remaining DNA fragments ranged from 113 bp to 776 bp. The average G+C content was 38.59%, similar to that of the gene-coding sequences in Streptococcus mutans strain UA159 whose genome sequences were just complete. Of the twenty-nine DNA fragments, five potentially represented new DNA fragments in Streptococcus mutans, thus registered and obtained their gene's accession number in GenBank. The remaining DNA fragments showed high homology to known genes of Streptococcus mutans strain UA159. Their predicted functions of these fragments were associated to bacterial signal transduction, transcriptional regulation, stress-damage repair, biochemical metabolism, outer membrane protein synthesis, adhesion on tooth surface and hypothetical proteins.

CONCLUSIONThe gene analysis, identification and functional forecasting were carried out through bioinformatics associated software and database to find out new genes and new functions of known genes, and to supply the groundwork for researches in gene functions.

An approach for genome analysis based on assembly of fragments of DNA from the whole genome can be applied to obtain the complete nucleotide sequence of the genome of Zymomonas mobilis. However, the problem of fragment assembly raise thorny computational issues. Computer simulation studies of sequence assembly usually show some abnormal assemblage of artificial sequences containing repetitive or duplicated regions, and suggest methods to correct those abnormalities. In this paper, we describe five simulation studies which had been performed previous to the actual genome assembly process of Zymomonas mobilis ZM4.
Base Sequence ; Computer Simulation* ; DNA ; Genome ; Zymomonas*

Panfacial bone fracture is challenging. Even experienced surgeons find restoration of original facial architecture difficult because of the severe degree of fragmentation and loss of reference segments that could guide the start of facial reconstruction. To restore the facial contour, surgeons usually follow a general sequence for panfacial bone reduction. Among the sequences, the bottom-to-top and outside-in sequence is reported to be the most widely used in recent publications. However, a single sequence cannot be applied to all cases of panfacial fractures because of the variations in panfacial bone fracture patterns. In this article, we intend to find the reference and discuss the efficacy of inside-out sequence in facial bone fracture reconstruction.
Base Sequence ; Facial Bones ; Fractures, Bone* ; Surgeons